FastQCFastQC Report
Fri 27 May 2016
ERR765324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7019674
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA520290.7411882660077947No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT470550.6703302745967975No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG295830.4214298270831381No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA270750.38570167218591633No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC267860.38158467188077394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258490.368236473659603No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG232960.331867263351546No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG199120.28365989645673No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT192650.2744429442164978No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT179340.25548194973156874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172370.24555271370151946No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC171590.24444155098940495No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC156740.22328672243183942No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC144620.2060209633666749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128690.1833276018231046No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA118950.16945231359746907No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG109320.15573372780559325No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG95690.13631687169518128No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC88020.1253904383593882No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC84450.12030473210009468No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC78810.1122701709509587No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC72400.1031386927655045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC68950.027.5870951
ATCTACA79650.024.2754382
TCTACAC93200.023.1144243
TACACTC92150.022.9220145
CTACACT108250.020.8604854
AGTACTT294850.020.34782812-13
GAGTACT282300.019.6624412-13
CACTCTT115100.017.9708587
TACTTTT347000.017.48802814-15
GTACTTT324100.017.28841814-15
ACACTCT122650.017.1327976
ACTCTTT136550.016.9036798
AGTACTC24600.016.6374155
CATGGGG146800.016.2797874
CATGGGT66200.015.9184624
CGTTAAC5950.015.8260281
ACTTTTT364400.015.605268516-17
GTACTAG17050.015.46402551
GTATAAA39350.015.315251
GTATAAG22150.014.8793231
Produced by FastQC (version 0.11.5)