##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765324_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7019674 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.407690157691086 34.0 31.0 34.0 31.0 34.0 2 32.46656767821412 34.0 31.0 34.0 31.0 34.0 3 32.49664200930129 34.0 31.0 34.0 31.0 34.0 4 35.950143553675 37.0 37.0 37.0 35.0 37.0 5 35.966193729224464 37.0 37.0 37.0 35.0 37.0 6 36.053340938624785 37.0 37.0 37.0 35.0 37.0 7 36.01360519021254 37.0 37.0 37.0 35.0 37.0 8 36.03830363062444 37.0 37.0 37.0 35.0 37.0 9 37.76997407571918 39.0 38.0 39.0 35.0 39.0 10-11 37.705640888736426 39.0 38.0 39.0 35.0 39.0 12-13 37.68343173771318 39.0 38.0 39.0 35.0 39.0 14-15 39.10616041428705 41.0 39.0 41.0 36.0 41.0 16-17 39.069012606568336 41.0 39.0 41.0 36.0 41.0 18-19 39.06533088288715 41.0 39.0 41.0 36.0 41.0 20-21 39.037792638233626 41.0 39.0 41.0 36.0 41.0 22-23 38.941105812036284 41.0 39.0 41.0 35.5 41.0 24-25 38.86403449789833 41.0 39.0 41.0 35.0 41.0 26-27 38.74333829462736 41.0 39.0 41.0 35.0 41.0 28-29 38.59968903114304 40.0 38.0 41.0 35.0 41.0 30-31 38.38009087031677 40.0 38.0 41.0 34.5 41.0 32-33 38.14966100989875 40.0 38.0 41.0 34.0 41.0 34-35 37.98983706365851 40.0 38.0 41.0 34.0 41.0 36-37 37.824931969775236 40.0 38.0 41.0 33.5 41.0 38-39 37.625849220348414 40.0 37.0 41.0 33.0 41.0 40-41 37.380930937818476 40.0 37.0 41.0 33.0 41.0 42-43 37.10874272793865 40.0 37.0 41.0 32.0 41.0 44-45 36.74331208258389 40.0 36.0 41.0 31.0 41.0 46-47 36.30887759175141 40.0 36.0 41.0 30.5 41.0 48-49 35.91354178270957 39.0 35.0 41.0 29.5 41.0 50-51 34.62967753203354 38.0 34.0 40.0 26.5 40.5 52-53 34.55682322284482 38.0 34.5 39.5 26.5 40.5 54-55 35.35874507847515 39.0 35.0 40.5 28.0 41.0 56-57 35.361502898852564 39.0 35.0 41.0 28.0 41.0 58-59 35.14256630721028 38.5 35.0 41.0 28.0 41.0 60-61 34.8114752195045 38.0 35.0 40.5 27.0 41.0 62-63 34.456812025743645 37.0 35.0 40.0 26.0 41.0 64-65 34.12720526622746 37.0 35.0 40.0 26.0 41.0 66-67 33.79937287686009 36.0 34.5 39.0 26.0 41.0 68-69 33.53455758771703 36.0 34.0 39.0 25.0 41.0 70-71 33.18326370996716 35.0 34.0 39.0 24.0 41.0 72-73 32.804281295684106 35.0 34.0 37.5 23.5 40.0 74-75 32.42140532452077 35.0 34.0 37.0 21.0 39.0 76-77 32.06438724932241 35.0 34.0 36.5 20.0 39.0 78-79 31.74290308923178 35.0 34.0 36.0 18.5 38.0 80-81 31.434440687701453 35.0 33.0 35.5 17.0 37.0 82-83 31.176980227286908 35.0 33.0 35.0 13.0 37.0 84-85 30.92755090905931 35.0 33.0 35.0 7.0 36.0 86-87 30.681677525195614 35.0 33.0 35.0 2.0 36.0 88-89 30.44993499698134 35.0 33.0 35.0 2.0 36.0 90-91 30.21420310971706 35.0 33.0 35.0 2.0 35.5 92-93 29.95284196958434 35.0 32.0 35.0 2.0 35.0 94-95 29.63733059398485 35.0 32.0 35.0 2.0 35.0 96-97 29.33065680827913 35.0 31.5 35.0 2.0 35.0 98-99 29.026426868256276 35.0 31.0 35.0 2.0 35.0 100 28.48721621545388 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4507870258261164 1101 2 0.5227380533530166 1101 3 0.49487649214793805 1101 4 0.42533792068903153 1101 5 0.47871834931916624 1101 6 0.4402348067570472 1101 7 0.4788499697934796 1101 8 0.42044627069100216 1101 9 0.501732107753206 1101 10-11 0.47636577779037026 1101 12-13 0.5274645822285535 1101 14-15 0.6817422688691224 1101 16-17 0.6472026878405401 1101 18-19 0.6590448057844966 1101 20-21 0.6377022838073145 1101 22-23 0.6932399509891312 1101 24-25 0.5710888875278997 1101 26-27 0.7095281965516591 1101 28-29 0.7056503497133306 1101 30-31 0.7446365880647008 1101 32-33 0.7430267787657314 1101 34-35 0.7251125504543268 1101 36-37 0.7191627828635418 1101 38-39 0.7760706129944737 1101 40-41 0.8205877015848841 1101 42-43 0.7407754630527492 1101 44-45 0.7714019562619754 1101 46-47 0.8745156868655712 1101 48-49 0.8345495265271481 1101 50-51 0.8072094732586237 1101 52-53 0.8422488347288777 1101 54-55 0.9204136809102295 1101 56-57 0.9503704687347962 1101 58-59 0.9249357462026282 1101 60-61 0.9600093364249531 1101 62-63 0.973394995694072 1101 64-65 0.8774266110890778 1101 66-67 0.8640879418953702 1101 68-69 0.8795516364719802 1101 70-71 0.7835066923265614 1101 72-73 0.8047672459864046 1101 74-75 0.7948745662780112 1101 76-77 0.7726799132319471 1101 78-79 0.7944579286148645 1101 80-81 0.7407926710851562 1101 82-83 0.7368575107870079 1101 84-85 0.8113293210465073 1101 86-87 0.7371195526150629 1101 88-89 0.8180310183131461 1101 90-91 0.8473437313886762 1101 92-93 0.8525966634887787 1101 94-95 0.889651661112314 1101 96-97 0.7926099097086379 1101 98-99 0.8659012252277698 1101 100 0.9110599997481756 1102 1 0.42885433387019134 1102 2 0.5163999204506453 1102 3 0.5687438713258786 1102 4 0.4547295011892203 1102 5 0.45177418188603724 1102 6 0.44333507036559894 1102 7 0.4862663244825498 1102 8 0.40371003234657366 1102 9 0.4161506890781439 1102 10-11 0.5030453918366717 1102 12-13 0.5161878960349995 1102 14-15 0.6656518847210648 1102 16-17 0.6821412748057156 1102 18-19 0.661297465450005 1102 20-21 0.6567729965540892 1102 22-23 0.6752061848621054 1102 24-25 0.6928678756661171 1102 26-27 0.6931899815893559 1102 28-29 0.6642724280404266 1102 30-31 0.6237959438503964 1102 32-33 0.6268757781527015 1102 34-35 0.6914338940899825 1102 36-37 0.6819394315089511 1102 38-39 0.6891601993303382 1102 40-41 0.7281250832367263 1102 42-43 0.6994591353715407 1102 44-45 0.7210492767157319 1102 46-47 0.6383192551258148 1102 48-49 0.6617666920010734 1102 50-51 0.6101767670778884 1102 52-53 0.6390472483501242 1102 54-55 0.7510310537702125 1102 56-57 0.7281658608830455 1102 58-59 0.7123514888749654 1102 60-61 0.6964347186550768 1102 62-63 0.6373794685157108 1102 64-65 0.6406037449146851 1102 66-67 0.6188085541318031 1102 68-69 0.5797891264265829 1102 70-71 0.5603297723144962 1102 72-73 0.5031611172665365 1102 74-75 0.4687258309769149 1102 76-77 0.4584579030505651 1102 78-79 0.4367511487739115 1102 80-81 0.4630848149840183 1102 82-83 0.45957262298076174 1102 84-85 0.4421841871304153 1102 86-87 0.43766563369897327 1102 88-89 0.45330690430789744 1102 90-91 0.5038862180512389 1102 92-93 0.5660347364677847 1102 94-95 0.49350815443494156 1102 96-97 0.45813034183168355 1102 98-99 0.49223350997025506 1102 100 0.5415352160292315 1103 1 0.42621701597036576 1103 2 0.47595947467875277 1103 3 0.5181570719959296 1103 4 0.4144625522993266 1103 5 0.4831555987721359 1103 6 0.4342490435528603 1103 7 0.4656975467279807 1103 8 0.3805092322039343 1103 9 0.4721914369697018 1103 10-11 0.4969552013130354 1103 12-13 0.5082870690321712 1103 14-15 0.6032594459772014 1103 16-17 0.6077237696192554 1103 18-19 0.6057063003838508 1103 20-21 0.6009140255209999 1103 22-23 0.5916307655256787 1103 24-25 0.5968769912926959 1103 26-27 0.5974980640554719 1103 28-29 0.5883810411290611 1103 30-31 0.6458908544197612 1103 32-33 0.6842164823486314 1103 34-35 0.6493955506056466 1103 36-37 0.6736087191478362 1103 38-39 0.6003795215201393 1103 40-41 0.6139366471957075 1103 42-43 0.650839297251963 1103 44-45 0.6075626490307684 1103 46-47 0.6022178078134814 1103 48-49 0.5835641356157311 1103 50-51 0.534536947584435 1103 52-53 0.5164803944274325 1103 54-55 0.6184807368430114 1103 56-57 0.6623765503173544 1103 58-59 0.7137739765870066 1103 60-61 0.7300593357444214 1103 62-63 0.700563785230166 1103 64-65 0.6452914359873816 1103 66-67 0.607334527009904 1103 68-69 0.5948199314591633 1103 70-71 0.595528610362912 1103 72-73 0.5428982241876668 1103 74-75 0.48888487998871 1103 76-77 0.47020452658789225 1103 78-79 0.4990567931619374 1103 80-81 0.45245566686503835 1103 82-83 0.3963165413686589 1103 84-85 0.4830491615290633 1103 86-87 0.5025394788450512 1103 88-89 0.5197031172517477 1103 90-91 0.5142191542211911 1103 92-93 0.5341088766932423 1103 94-95 0.49833111063141544 1103 96-97 0.6215820053646617 1103 98-99 0.5456217574543096 1103 100 0.537609037911789 1104 1 0.36063792375554726 1104 2 0.45161178833570403 1104 3 0.47086726976413473 1104 4 0.38786237753210173 1104 5 0.36801412771419706 1104 6 0.35659373675602524 1104 7 0.34665796295522 1104 8 0.3311758586942304 1104 9 0.3916167139355764 1104 10-11 0.40955832904376877 1104 12-13 0.46113828587410666 1104 14-15 0.533712032833499 1104 16-17 0.532164263386889 1104 18-19 0.5277601166371966 1104 20-21 0.5521929399553684 1104 22-23 0.556449318975325 1104 24-25 0.4995874903837034 1104 26-27 0.47936767163272975 1104 28-29 0.5136768899051916 1104 30-31 0.5514291676146854 1104 32-33 0.5776283604936836 1104 34-35 0.5448748045303731 1104 36-37 0.482779980987317 1104 38-39 0.5078787431028857 1104 40-41 0.5019544453295239 1104 42-43 0.5204257819196556 1104 44-45 0.5591942115904089 1104 46-47 0.44949425140082866 1104 48-49 0.4316011179682562 1104 50-51 0.3977033480826222 1104 52-53 0.4699216013257086 1104 54-55 0.5252192187787159 1104 56-57 0.44594541764492135 1104 58-59 0.4363849387107237 1104 60-61 0.4925409299429546 1104 62-63 0.49038168581373043 1104 64-65 0.44975292191058713 1104 66-67 0.4621174239273742 1104 68-69 0.5117996725224216 1104 70-71 0.509971112949259 1104 72-73 0.43333102417962266 1104 74-75 0.40826656558849095 1104 76-77 0.3666293314813771 1104 78-79 0.4240607605789002 1104 80-81 0.4358759820486924 1104 82-83 0.42377890112800287 1104 84-85 0.41915114925517827 1104 86-87 0.4295038524451549 1104 88-89 0.42157945437170596 1104 90-91 0.3927492011271063 1104 92-93 0.4719412608597331 1104 94-95 0.40393581528484646 1104 96-97 0.3388189756642781 1104 98-99 0.3323956950403151 1104 100 0.4094293289896207 1105 1 0.32955105897654136 1105 2 0.35968099905601036 1105 3 0.371459179724269 1105 4 0.31571583453622054 1105 5 0.31419668882962526 1105 6 0.34221583086390694 1105 7 0.24416607273749236 1105 8 0.27747325179090865 1105 9 0.31563760828472454 1105 10-11 0.33298387209580227 1105 12-13 0.3030014257137381 1105 14-15 0.4155538182715688 1105 16-17 0.4015510352497955 1105 18-19 0.4215745851045938 1105 20-21 0.39460361462042215 1105 22-23 0.4202163875539284 1105 24-25 0.3997483276066518 1105 26-27 0.3204999801721371 1105 28-29 0.4050853204562941 1105 30-31 0.38280093887082955 1105 32-33 0.46403906413842577 1105 34-35 0.4800576753661332 1105 36-37 0.4937705245555435 1105 38-39 0.472991827247192 1105 40-41 0.5533334252810178 1105 42-43 0.463315345793589 1105 44-45 0.5400543103748916 1105 46-47 0.46698538995811134 1105 48-49 0.517426884888323 1105 50-51 0.47730609100931787 1105 52-53 0.45330247944623636 1105 54-55 0.5514549757188973 1105 56-57 0.5464869754113906 1105 58-59 0.5388836210409096 1105 60-61 0.5204986232328892 1105 62-63 0.5156343976778359 1105 64-65 0.45420066883104226 1105 66-67 0.4552970562839107 1105 68-69 0.4770298526854688 1105 70-71 0.5155030621406311 1105 72-73 0.4660534296379524 1105 74-75 0.5350604530495247 1105 76-77 0.5238912769959541 1105 78-79 0.5497146598853782 1105 80-81 0.5290691080686436 1105 82-83 0.49727318035808565 1105 84-85 0.40328903094926005 1105 86-87 0.5128936055926197 1105 88-89 0.5503652420122336 1105 90-91 0.5727018736875173 1105 92-93 0.624001716094277 1105 94-95 0.5680536285506577 1105 96-97 0.5863950065711059 1105 98-99 0.5982936757000026 1105 100 0.6041479634671063 1106 1 0.2166067567909593 1106 2 0.24586171665691836 1106 3 0.2500329956103329 1106 4 0.2739249157869992 1106 5 0.25632190218851747 1106 6 0.29072140718400874 1106 7 0.25085645731623885 1106 8 0.2955271599425586 1106 9 0.334519056121664 1106 10-11 0.2993911564395617 1106 12-13 0.3286277547600207 1106 14-15 0.40667707685491195 1106 16-17 0.43954838168490085 1106 18-19 0.408480176101385 1106 20-21 0.4505094546976238 1106 22-23 0.4786060579845568 1106 24-25 0.42760169192097663 1106 26-27 0.3724748538620233 1106 28-29 0.39028294659529905 1106 30-31 0.44870522895755016 1106 32-33 0.42715301854519083 1106 34-35 0.37586409260898535 1106 36-37 0.40869080588704776 1106 38-39 0.47224212813116395 1106 40-41 0.41628813521758445 1106 42-43 0.4562347705142571 1106 44-45 0.4634345997174236 1106 46-47 0.5044774724232539 1106 48-49 0.4364103874857861 1106 50-51 0.40621769427572474 1106 52-53 0.34186648970204203 1106 54-55 0.4067504394212449 1106 56-57 0.4592327071162643 1106 58-59 0.4319235522297973 1106 60-61 0.42694140917967616 1106 62-63 0.411535496054249 1106 64-65 0.41314353768703427 1106 66-67 0.44639405440628366 1106 68-69 0.43378798760709003 1106 70-71 0.40738281721862535 1106 72-73 0.3530867360875334 1106 74-75 0.4062006886151579 1106 76-77 0.421988822436667 1106 78-79 0.4111788269983414 1106 80-81 0.45270601609399463 1106 82-83 0.39250870011881744 1106 84-85 0.33116228171208206 1106 86-87 0.3617457541405109 1106 88-89 0.34940628242041427 1106 90-91 0.3671496384201305 1106 92-93 0.3757943188999242 1106 94-95 0.18372061015469043 1106 96-97 0.22302101127188223 1106 98-99 0.26988772448918397 1106 100 0.329548403156366 1107 1 0.1900681923000036 1107 2 0.21282659567906848 1107 3 0.28097818934311647 1107 4 0.23482304765325068 1107 5 0.2567821183019774 1107 6 0.21769938708499126 1107 7 0.25169893640981655 1107 8 0.2311518430860673 1107 9 0.28385970638588987 1107 10-11 0.2721941816987865 1107 12-13 0.2618324584560483 1107 14-15 0.294499063669285 1107 16-17 0.3463716812713926 1107 18-19 0.33888435352685065 1107 20-21 0.30272728844715857 1107 22-23 0.29174469667510294 1107 24-25 0.319210038217534 1107 26-27 0.2658609518370767 1107 28-29 0.2742889592175004 1107 30-31 0.2539355189709269 1107 32-33 0.2926191006154184 1107 34-35 0.32559508059222964 1107 36-37 0.3044764104361519 1107 38-39 0.3598683740336739 1107 40-41 0.33658718394293174 1107 42-43 0.24637726787391045 1107 44-45 0.2415921383574755 1107 46-47 0.23652238838942452 1107 48-49 0.22693872315272756 1107 50-51 0.2949505644432122 1107 52-53 0.32811921371631314 1107 54-55 0.36332331649208527 1107 56-57 0.30214408525065295 1107 58-59 0.20838054425622943 1107 60-61 0.26229651485468963 1107 62-63 0.30464116736801117 1107 64-65 0.32276441527429256 1107 66-67 0.3040642730926564 1107 68-69 0.2470384845335616 1107 70-71 0.23574151366621265 1107 72-73 0.3003608472468926 1107 74-75 0.29222891728630884 1107 76-77 0.32633983278892487 1107 78-79 0.26677687406443695 1107 80-81 0.2715445843737001 1107 82-83 0.26611711127730686 1107 84-85 0.24809240415372713 1107 86-87 0.19501610304359573 1107 88-89 0.20241436248380396 1107 90-91 0.2369494680629387 1107 92-93 0.26499544691674615 1107 94-95 0.24850856377074848 1107 96-97 0.21345272069674692 1107 98-99 0.22135202008394828 1107 100 0.2856249478088131 1108 1 0.13665265752980105 1108 2 0.16290235305916667 1108 3 0.2157907883461334 1108 4 0.16075173373968 1108 5 0.1706127223673093 1108 6 0.1281776458499735 1108 7 0.17758452835256122 1108 8 0.19501191529760575 1108 9 0.21374568040642572 1108 10-11 0.24217419509734128 1108 12-13 0.2524252193397487 1108 14-15 0.2549610928756536 1108 16-17 0.25994762581629516 1108 18-19 0.24819653975087164 1108 20-21 0.27513796568047866 1108 22-23 0.23708061896184063 1108 24-25 0.3036348065901606 1108 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4040.0 8 4987.0 9 6267.0 10 8131.0 11 12108.0 12 19591.0 13 28967.0 14 31053.0 15 38864.0 16 52324.0 17 73339.0 18 74432.0 19 42620.0 20 35226.0 21 31280.0 22 32074.0 23 36461.0 24 41128.0 25 44051.0 26 48448.0 27 56009.0 28 65533.0 29 77938.0 30 96045.0 31 120585.0 32 156881.0 33 213192.0 34 315039.0 35 515145.0 36 958389.0 37 1740129.0 38 1620558.0 39 387574.0 40 1022.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32714619650415 18.37477695582032 13.557393375436375 23.740683472239155 2 15.454956881588725 21.68540516849509 37.42330385629996 25.436334093616225 3 18.493164775120157 26.763206872768187 29.91314467202178 24.830483680089877 4 13.203989163583765 17.844697845317903 35.615631824729164 33.33568116636916 5 13.89820848848996 36.450966266661844 33.42129809521945 16.229527149628744 6 30.788396829272223 35.297213277262024 18.00861046855007 15.905779424915679 7 25.889296811713013 32.37469408166842 22.138225705075033 19.597783401543538 8 27.112805164262316 31.31070863081591 21.187203367510836 20.389282837410942 9 27.321864908899425 14.632261774015303 19.92511941239765 38.12075390468762 10-11 24.833188062211732 25.450686700518048 26.78191984888948 22.93420538838074 12-13 26.643575047085356 23.642085751145565 27.007813181072297 22.706526020696778 14-15 24.829022640279845 23.51475670333918 25.589116238777056 26.06710441760392 16-17 22.816311683293847 26.646956082288785 26.84390260153889 23.692829632878475 18-19 23.611933435993727 25.563608274536215 28.956980956739503 21.867477332730555 20-21 24.326059292864286 24.902511946056052 28.137727111389438 22.633701649690227 22-23 23.37645297441475 25.481801449970646 27.025231361444412 24.11651421417019 24-25 23.049744214066738 26.17432694682666 26.744261810496106 24.0316670286105 26-27 23.54947984648667 25.337557057601185 27.029692004207075 24.08327109170508 28-29 23.028658176349268 24.844672078837114 28.747301267494112 23.379368477319513 30-31 24.076366450936217 24.88854228309421 27.99486223002839 23.04022903594118 32-33 22.329344545162574 25.674232792965768 27.936667865727895 24.059754796143768 34-35 22.41626952799117 25.999117017822932 28.198354691329676 23.386258762856226 36-37 23.29472968422245 26.032244058319982 27.26614990176645 23.40687635569112 38-39 23.634823895584105 25.05951391125197 27.244200709489107 24.06146148367482 40-41 23.069440208909082 25.7400057626256 27.277283468915225 23.91327055955009 42-43 24.240343848360656 25.40940602493401 26.943269741411296 23.406980385294037 44-45 23.87006649050743 25.295813717026487 27.257691655906346 23.576428136559738 46-47 23.189545023115578 25.430817881851304 27.950746623481333 23.428890471551785 48-49 23.595680442010845 26.02789775201811 27.23532386764056 23.14109793833049 50-51 22.87943575055885 26.46684426395081 26.246843855670253 24.406876129820084 52-53 22.70981426503265 26.52195276886335 25.599486905556773 25.168746060547225 54-55 23.280746978126167 25.974777084230364 25.61631540977436 25.128160527869102 56-57 23.576224266348735 25.16736438375234 25.891470203598555 25.364941146300367 58-59 23.361054910692154 25.004527536659854 27.072928825839803 24.56148872680819 60-61 23.298538732297693 25.427753810340054 26.560731360516517 24.712976096845736 62-63 22.130579966756006 25.568274867232297 26.94677223413221 25.35437293187948 64-65 22.30108510024283 25.40931124383597 26.8711577433218 25.418445912599402 66-67 23.305455111537015 25.33814951180606 26.173369446352226 25.18302593030471 68-69 23.49125278058746 24.67085359667803 26.24254237851298 25.595351244221533 70-71 22.884034829354665 24.985944689795755 26.429938370593902 25.70008211025568 72-73 23.813078421204956 24.69736960468061 26.360951310696308 25.128600663418133 74-75 23.905276595355975 24.385206475966896 26.59710439236714 25.112412536309993 76-77 23.11235006660535 25.06907050035711 27.01139961142715 24.807179821610386 78-79 23.27521016319763 24.891328612797288 26.947727828096667 24.885733395908414 80-81 22.94882361048456 24.93070488541342 26.625566241352416 25.494905262749608 82-83 23.12953308595041 25.1634698119292 25.87130937980155 25.83568772231884 84-85 23.37182205808484 24.99135234485593 25.751382768619575 25.885442828439654 86-87 23.482873102280134 24.248162258456123 26.211569272715906 26.057395366547837 88-89 23.728083277676514 23.738485709819376 26.709193445497426 25.82423756700668 90-91 24.149068902638845 24.14063541810926 26.185512828992408 25.524782850259488 92-93 23.701032430580575 24.274876761854067 26.341192733591946 25.68289807397341 94-95 23.44078009459539 24.24940427549206 26.63936465202083 25.67045097789172 96-97 24.299582090986135 24.03568695938597 25.92943725245982 25.73529369716808 98-99 24.50956624197235 23.655200220492627 25.831413534236784 26.003820003298234 100 24.597372172290928 23.74981596793142 26.066094732912777 25.586717126864876 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2747.0 1 3393.0 2 2637.0 3 1106.0 4 916.0 5 982.5 6 1272.5 7 2189.0 8 3397.5 9 3547.0 10 2810.0 11 2463.5 12 2684.5 13 2929.5 14 3303.0 15 3710.0 16 4177.5 17 4915.0 18 5689.0 19 6562.0 20 7664.5 21 9245.0 22 11340.5 23 13662.0 24 16311.0 25 19903.5 26 25090.5 27 31119.0 28 37330.0 29 45412.0 30 54746.0 31 63594.5 32 72878.0 33 83203.5 34 93207.0 35 100924.5 36 108355.5 37 117538.5 38 125340.5 39 132535.0 40 137956.5 41 142806.5 42 150026.5 43 164097.5 44 182001.5 45 199133.5 46 224335.5 47 253619.0 48 325007.0 49 395706.5 50 443943.5 51 423256.5 52 343041.0 53 312889.0 54 287518.5 55 254078.5 56 229425.5 57 204890.0 58 188486.5 59 172110.5 60 146915.0 61 122400.0 62 100932.0 63 79893.0 64 60670.0 65 48270.0 66 38109.5 67 28785.5 68 22502.5 69 18386.0 70 15680.0 71 13597.0 72 11503.0 73 11209.0 74 9309.0 75 7303.5 76 5810.5 77 3984.0 78 2901.5 79 2068.5 80 1453.5 81 1124.5 82 872.5 83 626.5 84 400.0 85 267.5 86 200.0 87 143.5 88 106.0 89 90.0 90 69.5 91 58.0 92 53.5 93 48.0 94 40.5 95 27.0 96 23.5 97 20.0 98 10.5 99 10.0 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08446261179650223 2 0.2798990380464962 3 0.2814660623840936 4 0.2893581667752662 5 0.31589786078384835 6 0.16657468708660828 7 0.18379770912438384 8 0.15071924992528143 9 0.20114894224432645 10-11 0.22122109944137008 12-13 0.23790990863678285 14-15 0.16604047424424553 16-17 0.15554141118234266 18-19 0.17241541416310785 20-21 0.20411916564786345 22-23 0.16821293980318744 24-25 0.19263715095601305 26-27 0.19056440512764553 28-29 0.2202381478114226 30-31 0.25362288904014635 32-33 0.2219974887722706 34-35 0.14918783977717484 36-37 0.14393118540832522 38-39 0.11208497716560628 40-41 0.10321704398238436 42-43 0.11863086519402469 44-45 0.13319706869578274 46-47 0.19802201640702974 48-49 0.2027871949609056 50-51 0.20922624042085144 52-53 0.13759185967895377 54-55 0.08282435907992308 56-57 0.12073210237398489 58-59 0.13948653455986704 60-61 0.11672194463731506 62-63 0.1379836157633531 64-65 0.11290410352389584 66-67 0.11635155706661023 68-69 0.10281104222218866 70-71 0.12790480013744226 72-73 0.15200848358485022 74-75 0.19000882377158823 76-77 0.17064895036436165 78-79 0.19502330165190007 80-81 0.10106594693713696 82-83 0.14093959349109375 84-85 0.17086263550130676 86-87 0.18485188913331302 88-89 0.16663879262769182 90-91 0.1694451907595709 92-93 0.12040445183066906 94-95 0.35372013002313213 96-97 0.35400504353905893 98-99 0.3996909258179226 100 0.4331112812361372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7019674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.69306194786634 #Duplication Level Percentage of deduplicated Percentage of total 1 79.54791207294329 34.75691850026536 2 11.232337230316098 9.815504128470533 3 3.3902398072960462 4.443898737549258 4 1.5819106867505806 2.7647408652873957 5 0.9402461678649718 2.0541117029384077 6 0.6070853643169217 1.591525105844536 7 0.42246826983712893 1.2921252599501112 8 0.3154232962484568 1.1025447698227215 9 0.25215143080374497 0.9915541277716045 >10 1.460834976295952 11.989699035896697 >50 0.12913238243571135 3.9328789942480817 >100 0.1000553991238656 8.947581081308325 >500 0.011801418137299652 3.5245302002910153 >1k 0.007322706105539225 5.707362552468888 >5k 5.230504360367481E-4 1.528553775758158 >10k+ 5.557410882890448E-4 5.556471162128977 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 52029 0.7411882660077947 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 47055 0.6703302745967975 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 29583 0.4214298270831381 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 27075 0.38570167218591633 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 26786 0.38158467188077394 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25849 0.368236473659603 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 23296 0.331867263351546 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19912 0.28365989645673 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 19265 0.2744429442164978 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 17934 0.25548194973156874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17237 0.24555271370151946 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 17159 0.24444155098940495 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 15674 0.22328672243183942 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14462 0.2060209633666749 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12869 0.1833276018231046 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 11895 0.16945231359746907 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 10932 0.15573372780559325 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 9569 0.13631687169518128 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 8802 0.1253904383593882 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 8445 0.12030473210009468 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 7881 0.1122701709509587 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7240 0.1031386927655045 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.84913515926808E-4 0.0 0.0 0.0 0.0 2 3.988789222975312E-4 0.0 0.0 0.0 0.0 3 3.988789222975312E-4 0.0 0.0 0.0 0.0 4 4.558616254828928E-4 0.0 0.0 4.27370273890212E-5 0.0 5 0.0018661835293205923 0.0 0.0 7.1228378981702E-5 0.0 6 0.0031198029993985475 0.0 0.0 7.1228378981702E-5 2.84913515926808E-5 7 0.0033904708395290153 0.0 0.0 8.54740547780424E-5 2.84913515926808E-5 8 0.003703875707048504 0.0 0.0 9.97197305743828E-5 2.84913515926808E-5 9 0.00391756084399361 0.0 0.0 1.42456757963404E-4 2.84913515926808E-5 10-11 0.006581502217909265 0.0 0.0 1.9231662325059539E-4 2.84913515926808E-5 12-13 0.013106021732633168 0.0 0.0 2.706678401304676E-4 3.5614189490851004E-5 14-15 0.020342825037174092 0.0 0.0 3.347733812139994E-4 4.27370273890212E-5 16-17 0.023220451548034853 0.0 0.0 4.344931117883822E-4 4.98598652871914E-5 18-19 0.025051020887864595 0.0 0.0 5.128443286682544E-4 5.69827031853616E-5 20-21 0.032622597573619516 0.0 0.0 0.00106842568472553 7.1228378981702E-5 22-23 0.035557206787665635 0.0 0.0 0.003525804759594249 8.54740547780424E-5 24-25 0.052060822197725995 0.0 0.0 0.009181338050741387 9.97197305743828E-5 26-27 0.10539663237922445 0.0 0.0 0.01918892529767052 1.139654063707232E-4 28-29 0.11757668518509548 0.0 0.0 0.057324599404473764 1.139654063707232E-4 30-31 0.11954258844499047 0.0 0.0 0.16247905529516043 1.139654063707232E-4 32-33 0.12087455913194829 0.0 0.0 0.3152496825351149 1.139654063707232E-4 34-35 0.12190737062718296 0.0 0.0 0.5120964306889465 1.282110821670636E-4 36-37 0.1237877998322999 7.1228378981702E-6 0.0 0.805564474931457 1.282110821670636E-4 38-39 0.1259388968775473 1.42456757963404E-5 0.0 1.204642836690137 1.282110821670636E-4 40-41 0.12776946621737706 1.42456757963404E-5 0.0 1.7117604036882623 1.282110821670636E-4 42-43 0.13200755476678833 1.42456757963404E-5 0.0 2.104328491608015 1.282110821670636E-4 44-45 0.13740666589360134 1.42456757963404E-5 0.0 2.521064368516259 1.353339200652338E-4 46-47 0.14031278375605477 1.42456757963404E-5 0.0 3.0043774112587 1.42456757963404E-4 48-49 0.14195815931053207 1.42456757963404E-5 0.0 3.4492057608373266 1.567024337597444E-4 50-51 0.14428020446533557 1.42456757963404E-5 0.0 3.9213145795659456 1.567024337597444E-4 52-53 0.14519192771630135 1.42456757963404E-5 0.0 4.446253486985293 1.567024337597444E-4 54-55 0.14784874625231884 1.42456757963404E-5 0.0 4.928263050392369 1.567024337597444E-4 56-57 0.15340455981289158 1.42456757963404E-5 0.0 5.438970527691172 1.567024337597444E-4 58-59 0.1556767451024079 1.42456757963404E-5 0.0 5.961060299951251 1.567024337597444E-4 60-61 0.15694461024828218 1.42456757963404E-5 0.0 6.446752370551681 1.567024337597444E-4 62-63 0.15764264836230285 1.42456757963404E-5 0.0 6.949154618861218 1.567024337597444E-4 64-65 0.15829794944893452 2.84913515926808E-5 0.0 7.479613725651647 1.709481095560848E-4 66-67 0.15924528688939116 2.84913515926808E-5 0.0 8.023997980532998 1.709481095560848E-4 68-69 0.16023536135723682 4.27370273890212E-5 0.0 8.637929624652084 1.709481095560848E-4 70-71 0.1616528060989727 4.98598652871914E-5 0.0 9.32477776033474 1.709481095560848E-4 72-73 0.16477260909837124 5.69827031853616E-5 0.0 9.923794181895058 1.994394611487656E-4 74-75 0.16745079614808323 5.69827031853616E-5 0.0 10.505181579657403 2.208079748432762E-4 76-77 0.16836964223694717 5.69827031853616E-5 0.0 11.133280548355948 2.421764885377868E-4 78-79 0.16914603156784774 5.69827031853616E-5 0.0 11.75588068619711 2.421764885377868E-4 80-81 0.17062758185066715 7.1228378981702E-5 0.0 12.441254394434841 2.49299326435957E-4 82-83 0.17114754901723356 7.1228378981702E-5 0.0 13.185989833715926 2.7066784013046757E-4 84-85 0.17344822565834256 8.54740547780424E-5 0.0 13.887838666012126 2.84913515926808E-4 86-87 0.1776863142077538 8.54740547780424E-5 0.0 14.583953613800299 2.991591917231484E-4 88 0.17868351151349762 8.54740547780424E-5 0.0 15.12546024217079 2.991591917231484E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 6895 0.0 27.587095 1 ATCTACA 7965 0.0 24.275438 2 TCTACAC 9320 0.0 23.114424 3 TACACTC 9215 0.0 22.922014 5 CTACACT 10825 0.0 20.860485 4 AGTACTT 29485 0.0 20.347828 12-13 GAGTACT 28230 0.0 19.66244 12-13 CACTCTT 11510 0.0 17.970858 7 TACTTTT 34700 0.0 17.488028 14-15 GTACTTT 32410 0.0 17.288418 14-15 ACACTCT 12265 0.0 17.132797 6 ACTCTTT 13655 0.0 16.903679 8 AGTACTC 2460 0.0 16.637415 5 CATGGGG 14680 0.0 16.279787 4 CATGGGT 6620 0.0 15.918462 4 CGTTAAC 595 0.0 15.826028 1 ACTTTTT 36440 0.0 15.6052685 16-17 GTACTAG 1705 0.0 15.4640255 1 GTATAAA 3935 0.0 15.31525 1 GTATAAG 2215 0.0 14.879323 1 >>END_MODULE