FastQCFastQC Report
Fri 27 May 2016
ERR765321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12209882
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196660.16106625764278476No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA150870.12356384770958474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137520.11263008110971096No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT137460.11258094058566659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA468000.023.1369971
GTACAAG61150.021.3472461
GTACATG702150.021.1592431
TACATGG695850.020.1895682
GTACTAG49800.020.0837041
TATCAAC564150.019.1098982
TACATGA47500.018.880822
ATCAACG511850.018.7232573
CAACGCA511650.018.7030435
AACGCAG529200.018.4909386
CATGGGG332150.018.4483434
GTACATA30350.017.947041
TAGTACT52600.017.853584
ACATGGG727650.017.7327163
TCAACGC560900.017.1529034
ACGCAGA556100.017.0307627
CGCAGAG557300.016.8255838
CTAGTAC59450.016.5862623
GTCCTAC49550.016.4891821
CGTTAAC13850.016.2736511
Produced by FastQC (version 0.11.5)