##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765321_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12209882
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17472273687821	33.0	33.0	33.0	33.0	33.0
2	32.100819893263505	33.0	33.0	33.0	33.0	33.0
3	32.12267686125058	33.0	33.0	33.0	33.0	33.0
4	35.89732824608788	37.0	37.0	37.0	33.0	37.0
5	35.925910422393926	37.0	37.0	37.0	33.0	37.0
6	35.90505256316155	37.0	37.0	37.0	33.0	37.0
7	35.84404239123687	37.0	37.0	37.0	33.0	37.0
8	35.788921383515415	37.0	37.0	37.0	33.0	37.0
9	35.782264071020506	37.0	37.0	37.0	33.0	37.0
10-11	35.77805366996995	37.0	37.0	37.0	33.0	37.0
12-13	35.7649105044586	37.0	37.0	37.0	33.0	37.0
14-15	37.61802906858559	40.0	37.0	40.0	33.0	40.0
16-17	37.672278282460056	40.0	37.0	40.0	33.0	40.0
18-19	37.72533383205505	40.0	37.0	40.0	33.0	40.0
20-21	37.74383429749771	40.0	37.0	40.0	33.0	40.0
22-23	37.74350792251718	40.0	37.0	40.0	33.0	40.0
24-25	37.75487715606097	40.0	37.0	40.0	33.0	40.0
26-27	37.745195326212	40.0	37.0	40.0	33.0	40.0
28-29	37.71216998657317	40.0	37.0	40.0	33.0	40.0
30-31	37.64829815718121	40.0	37.0	40.0	33.0	40.0
32-33	37.57178537843363	40.0	37.0	40.0	33.0	40.0
34-35	37.46867459488962	40.0	37.0	40.0	33.0	40.0
36-37	37.37039887035763	40.0	37.0	40.0	33.0	40.0
38-39	37.25375220661428	40.0	37.0	40.0	33.0	40.0
40-41	37.14356076496071	40.0	37.0	40.0	33.0	40.0
42-43	36.98540403584572	40.0	37.0	40.0	33.0	40.0
44-45	36.80980082362794	40.0	37.0	40.0	33.0	40.0
46-47	36.60482312605478	38.5	37.0	40.0	33.0	40.0
48-49	36.39799152031117	37.0	37.0	40.0	33.0	40.0
50-51	36.17519464971078	37.0	37.0	40.0	33.0	40.0
52-53	36.02224587428445	37.0	37.0	40.0	30.0	40.0
54-55	35.85287253390328	37.0	37.0	40.0	27.0	40.0
56-57	35.67616537981284	37.0	37.0	40.0	27.0	40.0
58-59	35.46357348089032	37.0	37.0	40.0	27.0	40.0
60-61	35.298358002149406	37.0	33.0	40.0	27.0	40.0
62-63	35.087819235271894	37.0	33.0	40.0	27.0	40.0
64-65	34.878392436552616	37.0	33.0	37.0	27.0	40.0
66-67	34.69108317344918	37.0	33.0	37.0	27.0	40.0
68-69	34.46436742795713	37.0	33.0	37.0	27.0	40.0
70-71	34.279263223018866	37.0	33.0	37.0	27.0	40.0
72-73	34.076021496358436	37.0	33.0	37.0	27.0	37.0
74-75	33.867743725942645	37.0	33.0	37.0	27.0	37.0
76-77	33.690160191556316	37.0	33.0	37.0	27.0	37.0
78-79	33.53661022276874	37.0	33.0	37.0	27.0	37.0
80-81	33.38370616521929	37.0	33.0	37.0	27.0	37.0
82-83	33.250525066499414	37.0	33.0	37.0	27.0	37.0
84-85	33.12176505882694	37.0	33.0	37.0	27.0	37.0
86-87	33.021873552913945	37.0	33.0	37.0	27.0	37.0
88-89	32.95724299383073	37.0	33.0	37.0	27.0	37.0
90-91	32.85039429537485	37.0	33.0	37.0	27.0	37.0
92-93	32.77744997863206	37.0	33.0	37.0	27.0	37.0
94-95	32.69787025787801	37.0	33.0	37.0	22.0	37.0
96-97	32.62468363740125	37.0	33.0	37.0	22.0	37.0
98-99	32.54532988115692	37.0	33.0	37.0	22.0	37.0
100	32.47614039185637	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	42.0
5	92.0
6	397.0
7	901.0
8	1609.0
9	2715.0
10	3279.0
11	4317.0
12	6728.0
13	11614.0
14	21339.0
15	29625.0
16	34515.0
17	41560.0
18	50093.0
19	58585.0
20	67323.0
21	80329.0
22	96242.0
23	93413.0
24	81046.0
25	78431.0
26	83958.0
27	94851.0
28	114034.0
29	143784.0
30	182962.0
31	236940.0
32	319751.0
33	442703.0
34	618078.0
35	917657.0
36	1767690.0
37	4093207.0
38	2426597.0
39	3470.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.468933925965345	18.843522111217958	12.913012895431866	24.774531067384828
2	16.65764103370525	21.136468061790367	36.820511938488096	25.38537896601628
3	18.345088732041038	25.768660076195854	29.45058870906778	26.435662482695328
4	13.484135227514892	17.49609865189524	36.134567066250106	32.88519905433975
5	14.569375492187048	37.11850615113516	33.00193660118915	15.310181755488642
6	31.515489989928625	36.55617311690813	17.528327327118518	14.400009566044735
7	27.560037025746848	31.79858740649582	21.94086724179644	18.70050832596089
8	27.083201950682245	32.22298954240508	20.174715857204845	20.519092649707833
9	27.203153969874567	14.620526226215782	19.62322813602949	38.55309166788016
10-11	25.345900148748367	25.349917386588995	27.118173623627158	22.18600884103548
12-13	26.388887296371905	23.597455732987427	26.731753017760536	23.281903952880135
14-15	24.320469326283284	24.266787225142338	24.716338478567597	26.696404970006782
16-17	23.734927078696362	26.60140817788269	25.36669097854562	24.296973764875325
18-19	23.81432995658063	25.91603437019389	27.402425590751527	22.867210082473953
20-21	25.26272515920298	25.140329381974023	26.44591710574679	23.151028353076203
22-23	24.91165805632241	24.757318219170596	26.304464492785147	24.02655923172185
24-25	24.244925372944795	25.37636934242684	26.497252927874666	23.881452356753698
26-27	24.265291656576057	25.461438523855513	26.31774622261649	23.95552359695194
28-29	23.994944486697996	25.34947675789665	26.798800927753636	23.85677782765172
30-31	24.410298472485568	25.148146448664143	26.63471985735988	23.806835221490413
32-33	24.068845994094062	25.382405015766256	26.22479354433488	24.323955445804803
34-35	24.28480424584754	25.36784182500383	26.52092871031676	23.826425218831872
36-37	24.334891157898888	25.284369121204943	26.199077007280536	24.18166271361564
38-39	24.36934300318397	25.09196349200463	26.4238796331827	24.114813871628694
40-41	24.262487349106966	25.11735669750076	26.576699480330156	24.04345647306212
42-43	24.493022894891745	25.212236813711574	26.15381035186393	24.14092993953275
44-45	24.2982444999535	25.45403780579087	25.92140502277287	24.32631267148276
46-47	24.119538985948367	25.478170446656218	25.891043187640744	24.511247379754675
48-49	24.677640464204288	25.51503377708533	25.545635196991213	24.26169056171917
50-51	24.15358298480862	25.808857744687984	25.344427133765773	24.69313213673762
52-53	24.185805896797643	25.792277156060216	25.11333519647867	24.90858175066347
54-55	24.251833914846593	25.685407888533017	25.13732414521484	24.925434051405546
56-57	24.374315209806767	25.41067746664582	25.057925507281432	25.157081816265976
58-59	24.081075530390645	25.40403736450202	25.53480091292769	24.980086192179645
60-61	24.23581467652723	25.47384922565023	25.29016812429451	25.000167973528026
62-63	24.10542812186685	25.381634736650486	25.222104813514868	25.2908323279678
64-65	23.84543496404244	25.38830552427974	25.38901419696693	25.37724531471089
66-67	24.357419250023728	25.31680430502138	25.24663297432348	25.079143470631415
68-69	24.156025808170472	25.06764119304109	25.409342327007785	25.366990671780655
70-71	23.92787742385449	25.24018134908618	25.316497286908596	25.51544394015074
72-73	24.438251092983933	25.18971428660378	25.35412630288719	25.01790831752509
74-75	24.49526780860761	25.20499307509666	25.357776430682737	24.941962685612992
76-77	24.18345465527483	25.34610010220631	25.334182109630536	25.13626313288832
78-79	24.176337887887712	25.450560527029698	25.324368921116868	25.048732663965716
80-81	24.00180194218792	25.289993158125302	25.562619457321606	25.145585442365174
82-83	24.216973623035955	25.158844509385553	25.486623802422475	25.137558065156018
84-85	24.318478278546543	25.248363364316123	25.12236542834239	25.310792928794946
86-87	23.920908161148663	25.124782205630652	25.449491204171427	25.50481842904925
88-89	24.054132201631827	24.710672708353805	25.523357810049934	25.711837279964435
90-91	24.174323663935912	24.951689330291792	25.28267051150472	25.59131649426758
92-93	24.084339748085277	24.85665012325339	25.359616195324037	25.699393933337294
94-95	23.554094051189022	24.936625412466235	25.698537570008572	25.81074296633617
96-97	23.980277167970126	24.919347502878114	25.30684891638815	25.793526412763608
98-99	23.720680025793488	25.00503849467267	25.229623686597236	26.044657792936604
100	23.754527644524035	25.012222377570026	25.19449531470749	26.038754663198453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	773.0
1	661.5
2	759.0
3	1020.0
4	876.5
5	782.5
6	1135.5
7	2239.5
8	3541.5
9	3549.5
10	2495.0
11	1878.0
12	1977.0
13	2156.5
14	2480.0
15	3015.0
16	3549.5
17	4295.5
18	5224.0
19	6169.0
20	7582.0
21	9456.0
22	12178.0
23	15910.0
24	20556.5
25	26513.0
26	34405.0
27	43537.0
28	53508.5
29	67106.0
30	82006.5
31	96264.0
32	113073.0
33	130470.5
34	147701.5
35	161801.5
36	176036.0
37	196673.0
38	213876.5
39	231189.5
40	244583.5
41	254919.5
42	271205.0
43	301128.5
44	342949.0
45	386552.5
46	435467.5
47	481556.5
48	517728.5
49	539323.5
50	559948.0
51	564917.0
52	553334.5
53	553522.0
54	543389.0
55	513120.0
56	483497.0
57	441929.0
58	404839.5
59	371417.0
60	315787.0
61	256046.5
62	207281.5
63	166560.0
64	126655.5
65	96560.0
66	75050.5
67	56204.5
68	43796.0
69	36254.5
70	31363.5
71	27304.0
72	23268.0
73	22565.0
74	18285.0
75	13698.5
76	10828.0
77	7931.0
78	6037.0
79	4083.5
80	2818.5
81	2152.0
82	1726.5
83	1392.0
84	1041.0
85	680.0
86	455.0
87	252.0
88	121.0
89	84.5
90	63.5
91	43.0
92	27.5
93	21.0
94	23.0
95	15.0
96	5.5
97	7.5
98	5.5
99	3.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02902566953554506
2	0.001736298516234637
3	2.53892707562612E-4
4	0.0
5	0.0028419603072331085
6	2.3751253288115316E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.2350844995881204E-4
16-17	0.003988572534935227
18-19	0.006674921182694476
20-21	0.019271275512736323
22-23	0.025004336651246916
24-25	0.031830774449744886
26-27	0.031236993117542
28-29	0.047338704829416045
30-31	0.04702748151046832
32-33	0.05798991341603465
34-35	0.05188420330352087
36-37	0.05618809420107418
38-39	0.06019304691069087
40-41	0.042670355045200274
42-43	0.049160999262728336
44-45	0.04641322495991362
46-47	0.04418552120323521
48-49	0.03739593879777053
50-51	0.036908628600997126
52-53	0.03861626181153921
54-55	0.05809638455146414
56-57	0.044341132862709076
58-59	0.044312467557016524
60-61	0.0456843071865887
62-63	0.030368843859424684
64-65	0.032473696305992145
66-67	0.03310842807489868
68-69	0.030692352309383497
70-71	0.04384972762226531
72-73	0.03244503100029959
74-75	0.04110195331945059
76-77	0.04623713808208793
78-79	0.04118794923652825
80-81	0.03409533359945657
82-83	0.021150900557433724
84-85	0.03379639541151995
86-87	0.027960958181250238
88-89	0.02659730863901879
90-91	0.02205181016491396
92-93	0.01557345107839699
94-95	0.015667637082815378
96-97	0.014721681994961131
98-99	0.011257275049832587
100	0.005888672797984452
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2209882E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.15384469119964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.32523612856984	26.128529009928254
2	15.237568963136072	11.32268541455603
3	5.773862306574019	6.4356355022046845
4	2.771263788090062	4.11852417524175
5	1.4997578024595832	2.786088423349911
6	0.8994058323766654	2.0049830766289056
7	0.6132491174323544	1.5949193726267863
8	0.4501108136016473	1.337867780990809
9	0.3365145432137366	1.1252528167403841
>10	1.7483351658026294	12.086881626194323
>50	0.1661923150490961	4.309389642093891
>100	0.14329749585934082	10.910733224064394
>500	0.0206008749139075	5.377495983774894
>1k	0.014206539599229148	9.20714669381094
>5k	3.097992504888895E-4	0.742064736861226
>10k+	8.851407156825413E-5	0.5118025209327502
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19666	0.16106625764278476	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	15087	0.12356384770958474	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13752	0.11263008110971096	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	13746	0.11258094058566659	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6380174681458838E-5
2	8.190087340729419E-6	3.2760349362917676E-5	0.0	0.0	1.6380174681458838E-5
3	8.190087340729419E-6	3.2760349362917676E-5	0.0	0.0	1.6380174681458838E-5
4	8.190087340729419E-6	3.2760349362917676E-5	0.0	0.0	1.6380174681458838E-5
5	8.190087340729419E-6	3.2760349362917676E-5	0.0	0.0	1.6380174681458838E-5
6	8.190087340729419E-6	3.2760349362917676E-5	0.0	1.6380174681458838E-5	2.457026202218826E-5
7	1.6380174681458838E-5	3.2760349362917676E-5	0.0	1.6380174681458838E-5	2.457026202218826E-5
8	1.6380174681458838E-5	3.2760349362917676E-5	0.0	1.6380174681458838E-5	3.2760349362917676E-5
9	1.6380174681458838E-5	8.190087340729419E-5	0.0	1.6380174681458838E-5	5.7330611385105935E-5
10-11	2.457026202218826E-5	8.190087340729419E-5	0.0	2.047521835182355E-5	5.7330611385105935E-5
12-13	4.914052404437652E-5	8.190087340729419E-5	0.0	2.8665305692552967E-5	6.552069872583535E-5
14-15	6.961574239620006E-5	1.5561165947385896E-4	0.0	3.2760349362917676E-5	8.190087340729419E-5
16-17	7.371078606656477E-5	1.6380174681458837E-4	0.0	3.6855393033282385E-5	8.190087340729419E-5
18-19	7.371078606656477E-5	1.719918341553178E-4	0.0	6.552069872583535E-5	8.190087340729419E-5
20-21	1.0647113542948245E-4	2.088472271886002E-4	0.0	3.153183626180826E-4	8.190087340729419E-5
22-23	1.26946353781306E-4	2.2522740187005903E-4	0.0	0.0011875626644057657	9.00909607480236E-5
24-25	1.5561165947385896E-4	2.53892707562612E-4	0.0	0.0037142046090207917	9.00909607480236E-5
26-27	2.4160757655151786E-4	2.702728822440708E-4	0.0	0.006445598737154053	9.828104808875303E-5
28-29	2.579877512329767E-4	2.702728822440708E-4	0.0	0.01992238745632431	9.828104808875303E-5
30-31	2.620827949033414E-4	3.1941340628844733E-4	0.0	0.06171640315606654	9.828104808875303E-5
32-33	2.661778385737061E-4	3.1941340628844733E-4	0.0	0.12719615144519825	1.4742157213312954E-4
34-35	2.702728822440708E-4	3.6036384299209447E-4	0.0	0.21136158400220412	1.5561165947385896E-4
36-37	2.7436792591443553E-4	4.0131427969574156E-4	0.0	0.34055611675854036	1.6380174681458837E-4
38-39	2.7846296958480024E-4	4.258845417179298E-4	0.0	0.5236209489985243	1.719918341553178E-4
40-41	2.8255801325516495E-4	4.586448910808475E-4	0.0	0.7244050352001763	1.801819214960472E-4
42-43	2.8665305692552966E-4	4.8321515310303573E-4	0.0	0.9107540924637929	1.801819214960472E-4
44-45	2.948431442662591E-4	5.487358518288711E-4	0.0	1.1297160775181938	1.801819214960472E-4
46-47	2.948431442662591E-4	5.896862885325182E-4	0.0	1.3717372534804186	1.8427696516641192E-4
48-49	2.948431442662591E-4	5.896862885325182E-4	0.0	1.6132915944642217	1.9656209617750607E-4
50-51	2.948431442662591E-4	6.674921182694476E-4	0.0	1.8500055938296538	1.9656209617750607E-4
52-53	3.030332316069885E-4	6.797772492805417E-4	0.0	2.078525410810686	2.047521835182355E-4
54-55	3.030332316069885E-4	6.797772492805417E-4	0.0	2.3196702474274526	2.170373145293296E-4
56-57	3.030332316069885E-4	6.879673366212712E-4	0.0	2.595913703342915	2.2932244554042374E-4
58-59	3.112233189477179E-4	6.961574239620006E-4	0.0	2.882714181840578	2.2932244554042374E-4
60-61	3.112233189477179E-4	7.248227296545535E-4	0.0	3.1684458539402756	2.2932244554042374E-4
62-63	3.112233189477179E-4	7.452979480063771E-4	0.0	3.46660598357953	2.3751253288115316E-4
64-65	3.153183626180826E-4	7.944384720507537E-4	0.0	3.7679684373690097	2.3751253288115316E-4
66-67	3.1941340628844733E-4	8.026285593914831E-4	0.0	4.096972435933451	2.457026202218826E-4
68-69	3.1941340628844733E-4	8.312938650840361E-4	0.0	4.464588601265762	2.457026202218826E-4
70-71	3.1941340628844733E-4	8.476740397654949E-4	0.0	4.853245100976405	2.579877512329767E-4
72-73	3.1941340628844733E-4	8.558641271062243E-4	0.0	5.229960453344267	2.702728822440708E-4
74-75	3.2760349362917675E-4	8.845294327987773E-4	0.0	5.617019886023469	2.702728822440708E-4
76-77	3.3169853729954146E-4	9.213848258320597E-4	0.0	6.039165652870356	2.8665305692552966E-4
78-79	3.3579358096990616E-4	9.336699568431538E-4	0.0	6.4809389640293	3.030332316069885E-4
80-81	3.3579358096990616E-4	9.336699568431538E-4	0.0	6.952982837999581	3.030332316069885E-4
82-83	3.3579358096990616E-4	9.336699568431538E-4	0.0	7.4482497046245	3.071282752773532E-4
84-85	3.3579358096990616E-4	9.336699568431538E-4	0.0	7.940101304828335	3.112233189477179E-4
86-87	3.3579358096990616E-4	9.500501315246126E-4	0.0	8.460220991488697	3.153183626180826E-4
88	3.3579358096990616E-4	9.910005682282597E-4	0.0	8.869045581275888	3.1941340628844733E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	46800	0.0	23.136997	1
GTACAAG	6115	0.0	21.347246	1
GTACATG	70215	0.0	21.159243	1
TACATGG	69585	0.0	20.189568	2
GTACTAG	4980	0.0	20.083704	1
TATCAAC	56415	0.0	19.109898	2
TACATGA	4750	0.0	18.88082	2
ATCAACG	51185	0.0	18.723257	3
CAACGCA	51165	0.0	18.703043	5
AACGCAG	52920	0.0	18.490938	6
CATGGGG	33215	0.0	18.448343	4
GTACATA	3035	0.0	17.94704	1
TAGTACT	5260	0.0	17.85358	4
ACATGGG	72765	0.0	17.732716	3
TCAACGC	56090	0.0	17.152903	4
ACGCAGA	55610	0.0	17.030762	7
CGCAGAG	55730	0.0	16.825583	8
CTAGTAC	5945	0.0	16.586262	3
GTCCTAC	4955	0.0	16.489182	1
CGTTAAC	1385	0.0	16.273651	1
>>END_MODULE