FastQCFastQC Report
Fri 27 May 2016
ERR765320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15896722
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1965641.236506494861016No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1810811.1391090565715372No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG785590.4941836436467845No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC545820.34335380589784487No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC545120.3429134635429871No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG538880.33898812597968314No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT499840.31442960378875595No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG497910.31321551701036227No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC475430.2990742368143571No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA461020.2900094749093555No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC344180.2165100452785172No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT305570.1922220191055741No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG277710.17469639338223314No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC215730.1357072231621085No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG198240.12470495489573258No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA194820.1225535679619987No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA194450.122320815574431No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT187990.11825708469960032No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC179610.11298555765144537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176240.11086562374305847No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC170680.10736804732447355No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC164530.10349932520679421No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG160010.1006559717154266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG64200.023.6458321
GTACAAG56100.022.7873171
GTACATA38950.022.6849331
TAGTACT74400.022.6145344
GTATAAG61400.022.2746941
TAGGCAT83650.021.4622365
GTGATCG44700.020.1869758
CATGGGG409850.019.5742954
TGATCGC46100.019.5739239
CTAGGCA95450.019.4983024
TACGGGC39350.019.4679584
CTATTGA69250.019.2741899
CTAGTAC92650.019.0221393
GGGCTAT77850.018.9560836
ATTTAGG75850.018.52691
ATGGGTA88150.018.287295
ATACGGG41200.018.2514023
AGGGCTA79300.018.1353445
CGTTAAC16900.018.0764771
CATGGGT120900.017.959464
Produced by FastQC (version 0.11.5)