FastQCFastQC Report
Fri 27 May 2016
ERR765317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12203456
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT940440.7706341547836941No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA913730.7487469123500752No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG368240.3017505860634889No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC273900.22444461634474694No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC271750.22268282034204082No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT234730.19234715149544523No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG230250.18867606028980644No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG225190.1845296938834376No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC171300.14037007221560843No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG153470.12575945699316654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139260.11411521457528097No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA133570.1094526009681192No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT131840.10803496976594172No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA124950.10238902815726955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG50450.021.525831
GTATAAG53650.021.3810581
GTACTAG53700.021.3611491
TAGGCAT74550.019.9836465
GTGATCG38050.019.8796968
CATGGGG294400.019.7485034
TAGTACT62050.019.4667574
ATAAGGT46950.018.4204623
CTGTGCG65550.018.3476669
GGGCTAT53650.018.0441936
CTAGGCA83150.017.9184024
CTATTGA51050.017.7613169
TGATCGC42750.017.6930759
CTAGTAC73600.017.6896923
CATGGGT101550.017.3098754
ATGGGGG145350.017.2982375
TCTAACG26650.017.2854612
CGTTAAC15350.017.1509721
TACGGGC37050.017.1256794
GTACATA32950.017.121231
Produced by FastQC (version 0.11.5)