##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765317_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12203456
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.455024461922918	33.0	33.0	33.0	27.0	33.0
2	31.374650426895464	33.0	33.0	33.0	27.0	33.0
3	31.32992850549877	33.0	33.0	33.0	27.0	33.0
4	34.9013015657204	37.0	37.0	37.0	33.0	37.0
5	35.133344767252815	37.0	37.0	37.0	33.0	37.0
6	35.138704232637046	37.0	37.0	37.0	33.0	37.0
7	34.98569003731402	37.0	37.0	37.0	33.0	37.0
8	34.968263580415254	37.0	37.0	37.0	33.0	37.0
9	34.689579329003195	37.0	37.0	37.0	27.0	37.0
10-11	34.68225513329995	37.0	37.0	37.0	27.0	37.0
12-13	34.66976191006876	37.0	37.0	37.0	27.0	37.0
14-15	35.876228586393886	37.0	37.0	40.0	27.0	40.0
16-17	36.090020482722274	37.0	37.0	40.0	27.0	40.0
18-19	35.894878098466535	37.0	37.0	40.0	27.0	40.0
20-21	35.870191771904615	37.0	37.0	40.0	27.0	40.0
22-23	36.03534089031828	37.0	37.0	40.0	27.0	40.0
24-25	36.057927074100974	37.0	37.0	40.0	27.0	40.0
26-27	35.73470748778051	37.0	37.0	40.0	27.0	40.0
28-29	35.59549286693867	37.0	37.0	40.0	27.0	40.0
30-31	35.3813804056818	37.0	33.0	40.0	27.0	40.0
32-33	35.30697803966352	37.0	33.0	40.0	27.0	40.0
34-35	35.25829633834874	37.0	33.0	40.0	27.0	40.0
36-37	35.03746885308556	37.0	33.0	40.0	27.0	40.0
38-39	34.912269401389246	37.0	33.0	40.0	27.0	40.0
40-41	35.024969893774355	37.0	33.0	40.0	27.0	40.0
42-43	34.92731182052035	37.0	33.0	40.0	27.0	40.0
44-45	34.86063140638193	37.0	33.0	40.0	27.0	40.0
46-47	34.78715562214507	37.0	33.0	40.0	27.0	40.0
48-49	34.60579228539849	37.0	33.0	40.0	27.0	40.0
50-51	34.447447141203284	37.0	33.0	40.0	27.0	40.0
52-53	34.33898196543667	37.0	33.0	40.0	24.5	40.0
54-55	34.190433144512504	37.0	33.0	40.0	24.5	40.0
56-57	33.967372357469884	37.0	33.0	37.0	22.0	40.0
58-59	33.77218756719408	37.0	33.0	37.0	22.0	40.0
60-61	33.48968255386015	37.0	33.0	37.0	22.0	40.0
62-63	33.23395794601136	37.0	33.0	37.0	22.0	40.0
64-65	33.05402940773499	37.0	33.0	37.0	22.0	40.0
66-67	32.83656838685697	37.0	33.0	37.0	22.0	40.0
68-69	32.63582230312463	37.0	33.0	37.0	22.0	38.5
70-71	32.37450083812323	35.0	33.0	37.0	22.0	37.0
72-73	32.12192845207128	33.0	33.0	37.0	22.0	37.0
74-75	31.89402895376523	33.0	33.0	37.0	22.0	37.0
76-77	31.674893284328633	33.0	33.0	37.0	22.0	37.0
78-79	31.47952633254055	33.0	30.0	37.0	22.0	37.0
80-81	31.343055647514934	33.0	27.0	37.0	22.0	37.0
82-83	31.211994823433624	33.0	27.0	37.0	22.0	37.0
84-85	31.026066304496037	33.0	27.0	37.0	22.0	37.0
86-87	30.80067277662983	33.0	27.0	37.0	15.0	37.0
88-89	30.639691330062565	33.0	27.0	37.0	15.0	37.0
90-91	30.52866143000802	33.0	27.0	37.0	15.0	37.0
92-93	30.394040425925247	33.0	27.0	37.0	15.0	37.0
94-95	30.250393249256604	33.0	27.0	37.0	15.0	37.0
96-97	30.147508582814574	33.0	27.0	37.0	15.0	37.0
98-99	29.99172689277529	33.0	27.0	37.0	15.0	37.0
100	29.870414823472956	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	11.0
7	172.0
8	1117.0
9	2604.0
10	4674.0
11	9474.0
12	18330.0
13	29962.0
14	40508.0
15	46041.0
16	52853.0
17	62308.0
18	73181.0
19	84742.0
20	98160.0
21	114366.0
22	130629.0
23	138046.0
24	145248.0
25	159395.0
26	184362.0
27	220101.0
28	267239.0
29	328145.0
30	404137.0
31	499194.0
32	635368.0
33	817134.0
34	1054128.0
35	1384770.0
36	1955404.0
37	2409844.0
38	831095.0
39	714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19181672316886	19.29482701358914	12.63490819933755	24.878448063904447
2	15.446539538141712	21.466829513666305	37.32478148888288	25.76184945930911
3	17.913332099527782	27.41914543829904	29.21091468759175	25.45660777458143
4	12.37236109938585	17.98322709564998	37.01081009751136	32.63360170745281
5	13.612473189837667	37.943290718459075	33.45561581211536	14.988620279587888
6	30.83478431498871	36.72101177989733	18.011581788440523	14.432622116673432
7	26.597142862530397	32.221538381824466	22.85523051410815	18.326088241536983
8	27.35356087853434	32.240941435004764	20.64839081540176	19.75710687105913
9	27.587075254329292	14.091714818160387	20.11531774331497	38.20589218419535
10-11	25.272937105511506	25.411711913659534	26.5530824703817	22.76226851044726
12-13	26.48951434607247	23.336611124495423	26.333589820491767	23.84028470894034
14-15	24.854980351833056	24.045457353963812	24.21388894397474	26.88567335022839
16-17	23.706331099386855	26.302318682381326	25.982309536804394	24.009040681427432
18-19	24.369835942086873	25.53624499699638	27.715696597201568	22.378222463715176
20-21	25.374148628504134	24.942597216770185	26.44708773787387	23.23616641685181
22-23	24.313917243224246	25.09575735143698	25.61794984604732	24.97237555929145
24-25	23.837988923891633	25.932353052552582	25.686933139589684	24.542724883966102
26-27	24.59251539730996	24.994398537796027	26.01175194352398	24.401334121370027
28-29	23.634715668693197	24.989243064681922	27.169011959351018	24.20702930727386
30-31	24.59815045176256	25.207071161682414	26.516610371577105	23.67816801497792
32-33	23.442729971761626	25.42581055551186	26.02029492140019	25.111164551326333
34-35	23.834746309849276	25.57705707307959	26.991469671295114	23.59672694577602
36-37	24.634286469921342	25.172933923611403	26.137614879716264	24.055164726750995
38-39	24.836261962003224	24.48453135962885	26.148093017840697	24.53111366052723
40-41	23.938433021975168	25.432567682870687	26.626173352727182	24.00282594242696
42-43	25.248907811438848	24.87067682279246	25.979362078084954	23.901053287683734
44-45	24.70905379955785	25.16047249307107	25.641203290138087	24.48927041723299
46-47	23.838929183569544	25.08717668166899	26.88000196133441	24.19389217342706
48-49	24.889147200120178	25.535379910478103	25.95223202879237	23.62324086060935
50-51	23.468828923650477	26.036482451027886	25.673748226331206	24.82094039899043
52-53	23.330372685934066	25.71154280458613	25.036854446936648	25.921230062543167
54-55	23.947617789583543	25.70418576508163	25.115106736976806	25.233089708358026
56-57	24.601768548188314	24.582999274959487	24.850517754970394	25.9647144218818
58-59	23.79787332375353	24.72220574237331	26.132138305738966	25.347782628134198
60-61	24.198341846768653	25.13482656060709	25.576750553285887	25.090081039338365
62-63	23.072929543050407	25.209444005722677	25.48434732964577	26.233279121581145
64-65	22.95843242092038	25.11253599295739	25.861518102821755	26.06751348330047
66-67	24.45570806268302	24.80235734293896	25.36388525476977	25.37804933960825
68-69	24.56775225106608	24.20024840056236	25.24244007151032	25.989559276861247
70-71	23.17539777181817	24.69630815390487	25.738845518858504	26.38944855541846
72-73	24.324909450577312	24.500614315570672	25.532337516737623	25.64213871711439
74-75	24.719426690016892	24.215040655919406	25.51191156960074	25.553621084462957
76-77	23.641662886653737	24.724492676922324	26.214092967135542	25.4197514692884
78-79	23.91209096832897	24.96297360354313	25.821291935661506	25.3036434924664
80-81	23.05749729902233	24.816868408089302	26.455384364005546	25.670249928882825
82-83	23.241344218425354	25.067353053572656	25.34697142444144	26.34433130356055
84-85	23.401691391260577	25.334001733552096	24.832086979983025	26.432219895204295
86-87	23.470484682434154	24.41403769164064	25.38866933302346	26.72680829290175
88-89	23.43749366195599	23.985682484061726	25.8871510009203	26.689672853061985
90-91	23.99575626387654	24.36419657202721	25.415665660200442	26.224381503895806
92-93	23.254298020919872	24.595490988976266	25.500809558875225	26.649401431228632
94-95	22.357564164628076	24.88660549496408	26.275160394924807	26.480669945483037
96-97	23.67495092547412	24.472222214255225	25.42119646662594	26.43163039364471
98-99	23.34362867918962	24.43591188178244	25.42667024504371	26.79378919398423
100	22.916301321144044	24.847644771065617	25.4376279392218	26.79842596856854
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1040.0
1	930.5
2	995.0
3	1164.0
4	982.5
5	869.5
6	1107.0
7	2015.0
8	3276.0
9	3301.5
10	2271.0
11	1636.5
12	1537.0
13	1648.0
14	1904.0
15	2283.5
16	2716.0
17	3290.5
18	4064.0
19	4956.5
20	6088.5
21	7631.0
22	9706.0
23	12426.0
24	15713.0
25	20115.5
26	26927.5
27	34787.0
28	43134.5
29	56268.5
30	71577.0
31	85197.0
32	101585.0
33	118139.5
34	136271.0
35	153094.5
36	167989.0
37	190839.0
38	205608.0
39	214489.5
40	220832.0
41	225077.5
42	238355.5
43	270182.5
44	318653.5
45	371328.5
46	434152.5
47	495300.5
48	562850.5
49	638513.0
50	771505.0
51	777718.5
52	626222.5
53	551875.0
54	513011.5
55	475262.0
56	450863.0
57	415030.5
58	383895.5
59	354370.0
60	302747.0
61	236168.0
62	181438.5
63	138482.0
64	100389.0
65	81277.5
66	66584.0
67	48447.0
68	35610.5
69	28756.5
70	25344.5
71	24018.0
72	22554.5
73	24453.5
74	20130.5
75	14314.0
76	10615.0
77	7722.0
78	5819.0
79	3512.5
80	2381.5
81	1917.0
82	1724.0
83	1568.5
84	1178.5
85	826.5
86	593.0
87	314.0
88	153.5
89	107.5
90	85.0
91	58.5
92	36.5
93	23.5
94	18.5
95	14.5
96	8.0
97	5.5
98	3.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04912542807545666
2	0.024968336838351365
3	0.02230515683426072
4	0.023157374435569728
5	0.01578241442424179
6	0.02536166803895552
7	0.04919098327555735
8	0.005924551209100111
9	0.003908728806003807
10-11	0.0019051980029263843
12-13	9.505504014600454E-4
14-15	0.0023108208035494205
16-17	0.006850518410522397
18-19	0.005420595608326035
20-21	0.008899118413669046
22-23	0.018879897628999522
24-25	0.02574680483954709
26-27	0.028815607644260773
28-29	0.04516343566937104
30-31	0.04288948966587826
32-33	0.04085318126275048
34-35	0.048502653674500074
36-37	0.0457985016703465
38-39	0.05542282448512945
40-41	0.05296040728134719
42-43	0.05795899128902501
44-45	0.058417877689729863
46-47	0.06291250609663361
48-49	0.06558797770074314
50-51	0.03413787045243577
52-53	3.605536005538103E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.145800009439949E-4
64-65	2.2124880033983814E-4
66-67	4.547892006985562E-4
68-69	0.0014954780022970542
70-71	6.965240010698609E-5
72-73	3.0729000047199743E-4
74-75	4.506920006922629E-5
76-77	1.1881880018250568E-4
78-79	0.0
80-81	3.687480005663969E-5
82-83	3.0729000047199743E-4
84-85	0.0015446444023725738
86-87	0.00440858720677159
88-89	0.0029622756045500553
90-91	0.004338934806664603
92-93	0.004211921606469511
94-95	0.0030606084047010945
96-97	0.002724638004185044
98-99	0.0043512264066834835
100	0.003244982404984293
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2203456E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.94698628286856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.40191775301534	25.257208813807264
2	13.075531771949004	8.877497954071297
3	5.104157267918024	5.19812270278851
4	2.4885210492455925	3.379111596934792
5	1.3381527632487344	2.271312674919372
6	0.7601035266084549	1.5481934394802341
7	0.5374007967647991	1.277019623332409
8	0.3713492898920881	1.0084951400095754
9	0.26870193299460876	0.8209458750193424
>10	1.3615469354215035	8.396971795480427
>50	0.11762460210929895	2.7974247452494176
>100	0.12389140383378339	9.249529228895918
>500	0.025848103281478525	6.203493838201099
>1k	0.02273523034571646	14.768786411827845
>5k	0.001901024790865359	4.251338341861618
>10k+	6.165485808211975E-4	4.6945478181209515
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	94044	0.7706341547836941	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	91373	0.7487469123500752	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	36824	0.3017505860634889	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	27390	0.22444461634474694	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	27175	0.22268282034204082	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	23473	0.19234715149544523	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	23025	0.18867606028980644	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	22519	0.1845296938834376	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	17130	0.14037007221560843	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	15347	0.12575945699316654	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13926	0.11411521457528097	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	13357	0.1094526009681192	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	13184	0.10803496976594172	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	12495	0.10238902815726955	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.6388800025173197E-5	0.0	0.0	0.0	0.0
3	1.6388800025173197E-5	0.0	0.0	0.0	0.0
4	1.6388800025173197E-5	0.0	0.0	0.0	0.0
5	1.6388800025173197E-5	0.0	0.0	0.0	0.0
6	1.6388800025173197E-5	0.0	0.0	0.0	0.0
7	1.6388800025173197E-5	0.0	0.0	0.0	0.0
8	1.6388800025173197E-5	0.0	0.0	0.0	0.0
9	1.6388800025173197E-5	0.0	0.0	8.194400012586599E-6	0.0
10-11	1.6388800025173197E-5	0.0	0.0	3.687480005663969E-5	0.0
12-13	1.6388800025173197E-5	0.0	0.0	8.194400012586598E-5	0.0
14-15	1.6388800025173197E-5	0.0	0.0	8.604120013215928E-5	0.0
16-17	1.6388800025173197E-5	8.194400012586599E-6	0.0	9.013840013845259E-5	0.0
18-19	1.6388800025173197E-5	8.194400012586599E-6	0.0	1.3930480021397216E-4	0.0
20-21	3.2777600050346394E-5	8.194400012586599E-6	0.0	3.031928004657041E-4	0.0
22-23	3.687480005663969E-5	8.194400012586599E-6	0.0	0.0013807564021208417	4.097200006293299E-6
24-25	4.9166400075519585E-5	8.194400012586599E-6	0.0	0.004441364806821936	8.194400012586599E-6
26-27	8.194400012586598E-5	8.194400012586599E-6	0.0	0.007161905611000687	8.194400012586599E-6
28-29	8.604120013215928E-5	8.194400012586599E-6	0.0	0.01952315802998757	8.194400012586599E-6
30-31	9.013840013845259E-5	8.194400012586599E-6	0.0	0.061519458094493884	8.194400012586599E-6
32-33	9.013840013845259E-5	1.2291600018879898E-5	0.0	0.1459053894241107	8.194400012586599E-6
34-35	9.013840013845259E-5	1.6388800025173197E-5	0.0	0.25843089039694983	8.194400012586599E-6
36-37	9.013840013845259E-5	1.6388800025173197E-5	0.0	0.42855073185825393	8.194400012586599E-6
38-39	1.0243000015733249E-4	1.6388800025173197E-5	0.0	0.7018585554780548	8.194400012586599E-6
40-41	1.0652720016362578E-4	1.6388800025173197E-5	0.0	1.0592655064270318	8.194400012586599E-6
42-43	1.0652720016362578E-4	1.6388800025173197E-5	0.0	1.3644905180958573	8.194400012586599E-6
44-45	1.0652720016362578E-4	1.6388800025173197E-5	0.0	1.716554720236628	8.194400012586599E-6
46-47	1.0652720016362578E-4	1.6388800025173197E-5	0.0	2.10895175923935	8.194400012586599E-6
48-49	1.0652720016362578E-4	1.6388800025173197E-5	0.0	2.5014307422421975	8.194400012586599E-6
50-51	1.0652720016362578E-4	1.6388800025173197E-5	0.0	2.9343122145071034	8.194400012586599E-6
52-53	1.0652720016362578E-4	1.6388800025173197E-5	0.0	3.3932764620120723	8.194400012586599E-6
54-55	1.0652720016362578E-4	1.6388800025173197E-5	0.0	3.833545185888325	8.194400012586599E-6
56-57	1.0652720016362578E-4	1.6388800025173197E-5	0.0	4.322418993439236	8.194400012586599E-6
58-59	1.0652720016362578E-4	1.6388800025173197E-5	0.0	4.857918937061763	8.194400012586599E-6
60-61	1.0652720016362578E-4	1.6388800025173197E-5	0.0	5.386560167873757	8.194400012586599E-6
62-63	1.0652720016362578E-4	1.6388800025173197E-5	0.0	5.917159860288757	1.6388800025173197E-5
64-65	1.1062440016991907E-4	1.6388800025173197E-5	0.0	6.478062443950304	1.6388800025173197E-5
66-67	1.1472160017621237E-4	1.6388800025173197E-5	0.0	7.073610950865066	1.6388800025173197E-5
68-69	1.1472160017621237E-4	2.4583200037759796E-5	0.0	7.729593157872655	1.6388800025173197E-5
70-71	1.1472160017621237E-4	2.4583200037759796E-5	0.0	8.507938243068192	1.6388800025173197E-5
72-73	1.1472160017621237E-4	2.4583200037759796E-5	0.0	9.203855858537123	1.6388800025173197E-5
74-75	1.1881880018250568E-4	2.4583200037759796E-5	0.0	9.875370550768569	1.6388800025173197E-5
76-77	1.2291600018879897E-4	2.4583200037759796E-5	0.0	10.599628498681028	1.6388800025173197E-5
78-79	1.2291600018879897E-4	2.4583200037759796E-5	0.0	11.325308174995673	1.6388800025173197E-5
80-81	1.2291600018879897E-4	2.4583200037759796E-5	0.0	12.105406861793906	1.6388800025173197E-5
82-83	1.2291600018879897E-4	2.4583200037759796E-5	0.0	12.938002972272773	1.6388800025173197E-5
84-85	1.2291600018879897E-4	2.4583200037759796E-5	0.0	13.733929142695315	1.6388800025173197E-5
86-87	1.2291600018879897E-4	2.4583200037759796E-5	0.0	14.543683363139097	1.6388800025173197E-5
88	1.2291600018879897E-4	2.4583200037759796E-5	0.0	15.181519071318814	1.6388800025173197E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAG	5045	0.0	21.52583	1
GTATAAG	5365	0.0	21.381058	1
GTACTAG	5370	0.0	21.361149	1
TAGGCAT	7455	0.0	19.983646	5
GTGATCG	3805	0.0	19.879696	8
CATGGGG	29440	0.0	19.748503	4
TAGTACT	6205	0.0	19.466757	4
ATAAGGT	4695	0.0	18.420462	3
CTGTGCG	6555	0.0	18.347666	9
GGGCTAT	5365	0.0	18.044193	6
CTAGGCA	8315	0.0	17.918402	4
CTATTGA	5105	0.0	17.761316	9
TGATCGC	4275	0.0	17.693075	9
CTAGTAC	7360	0.0	17.689692	3
CATGGGT	10155	0.0	17.309875	4
ATGGGGG	14535	0.0	17.298237	5
TCTAACG	2665	0.0	17.285461	2
CGTTAAC	1535	0.0	17.150972	1
TACGGGC	3705	0.0	17.125679	4
GTACATA	3295	0.0	17.12123	1
>>END_MODULE