FastQCFastQC Report
Fri 27 May 2016
ERR765316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10958392
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT233550.21312433430014185No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA227520.20762170216214204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132910.12128604269677522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG233450.018.8621434
GTATAAG39750.018.3293441
GTACATG734950.018.2408081
GTATATA26650.018.1673831
GTACTAG30000.018.0189271
TACATGG734000.017.6468872
TAGTACT41150.017.587184
CATGGGT77050.017.3827114
ACATGGG681300.016.6306823
CTAGTAC44100.016.5175023
GTACAAG55650.015.6263991
ATGGGTA54950.015.3926865
TATACCG21950.015.1996455
GTATCAA526700.015.1182821
GTATAGA28350.015.0883131
CCTAGTA46550.015.04359152
GTATAAA109450.014.8597911
GTATATG37350.014.5988871
GTATTAA38350.014.5859251
TAGGCAT49100.014.5469425
Produced by FastQC (version 0.11.5)