##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765316_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10958392
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61911729385114	33.0	33.0	33.0	27.0	33.0
2	31.52592825662743	33.0	33.0	33.0	27.0	33.0
3	31.476808002487957	33.0	33.0	33.0	27.0	33.0
4	35.10468871710375	37.0	37.0	37.0	33.0	37.0
5	35.311839547262046	37.0	37.0	37.0	33.0	37.0
6	35.31342572888431	37.0	37.0	37.0	33.0	37.0
7	35.16938945056903	37.0	37.0	37.0	33.0	37.0
8	35.155585509260845	37.0	37.0	37.0	33.0	37.0
9	34.89337459364476	37.0	37.0	37.0	33.0	37.0
10-11	34.88231179355512	37.0	37.0	37.0	33.0	37.0
12-13	34.86678323790571	37.0	37.0	37.0	33.0	37.0
14-15	36.137938440238315	37.0	37.0	40.0	30.0	40.0
16-17	36.36506309502343	37.0	37.0	40.0	33.0	40.0
18-19	36.17640078945889	37.0	37.0	40.0	27.0	40.0
20-21	36.15795287301275	37.0	37.0	40.0	27.0	40.0
22-23	36.32453976824337	37.0	37.0	40.0	33.0	40.0
24-25	36.33687168701394	37.0	37.0	40.0	33.0	40.0
26-27	36.013709265008956	37.0	37.0	40.0	27.0	40.0
28-29	35.89267640726851	37.0	37.0	40.0	27.0	40.0
30-31	35.701490875668625	37.0	37.0	40.0	27.0	40.0
32-33	35.66991512075859	37.0	37.0	40.0	27.0	40.0
34-35	35.62789025068642	37.0	37.0	40.0	27.0	40.0
36-37	35.40614640359644	37.0	33.0	40.0	27.0	40.0
38-39	35.286014134190495	37.0	33.0	40.0	27.0	40.0
40-41	35.43867366672045	37.0	33.0	40.0	27.0	40.0
42-43	35.37178041267369	37.0	33.0	40.0	27.0	40.0
44-45	35.332825336052956	37.0	33.0	40.0	27.0	40.0
46-47	35.30981265317028	37.0	33.0	40.0	27.0	40.0
48-49	35.15818105430067	37.0	33.0	40.0	27.0	40.0
50-51	35.016011245080485	37.0	33.0	40.0	27.0	40.0
52-53	34.901312391453054	37.0	33.0	40.0	27.0	40.0
54-55	34.77492149395641	37.0	33.0	40.0	27.0	40.0
56-57	34.574119177339156	37.0	33.0	40.0	27.0	40.0
58-59	34.385323229904536	37.0	33.0	38.5	27.0	40.0
60-61	34.114852023910075	37.0	33.0	37.0	27.0	40.0
62-63	33.889903555193136	37.0	33.0	37.0	27.0	40.0
64-65	33.687275240747	37.0	33.0	37.0	27.0	40.0
66-67	33.46368828565359	37.0	33.0	37.0	27.0	40.0
68-69	33.2607224673109	37.0	33.0	37.0	27.0	40.0
70-71	33.01807464087797	37.0	33.0	37.0	22.0	37.0
72-73	32.77413935365699	37.0	33.0	37.0	22.0	37.0
74-75	32.550748093333404	33.0	33.0	37.0	22.0	37.0
76-77	32.3485497689807	33.0	33.0	37.0	22.0	37.0
78-79	32.15699867279798	33.0	33.0	37.0	22.0	37.0
80-81	31.99925801157688	33.0	33.0	37.0	22.0	37.0
82-83	31.852651420025857	33.0	33.0	37.0	22.0	37.0
84-85	31.683585465823818	33.0	33.0	37.0	22.0	37.0
86-87	31.481319293925605	33.0	33.0	37.0	22.0	37.0
88-89	31.33248495764707	33.0	30.0	37.0	22.0	37.0
90-91	31.23683278532106	33.0	27.0	37.0	22.0	37.0
92-93	31.141323654054354	33.0	27.0	37.0	22.0	37.0
94-95	31.003476696216016	33.0	27.0	37.0	18.5	37.0
96-97	30.892290949256058	33.0	27.0	37.0	15.0	37.0
98-99	30.75532089927062	33.0	27.0	37.0	15.0	37.0
100	30.673334007398164	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	179.0
8	1035.0
9	2222.0
10	3861.0
11	6973.0
12	12259.0
13	19627.0
14	26392.0
15	30928.0
16	35850.0
17	42655.0
18	50540.0
19	60213.0
20	70682.0
21	83054.0
22	96262.0
23	104490.0
24	112110.0
25	125575.0
26	146980.0
27	176725.0
28	215960.0
29	268798.0
30	335267.0
31	416783.0
32	530974.0
33	695772.0
34	911384.0
35	1223470.0
36	1788260.0
37	2446882.0
38	915612.0
39	611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.402195143083645	18.322926898833828	12.688333122280026	25.586544835802506
2	15.93855002342158	20.55453650279836	37.44691808142386	26.059995392356207
3	18.051100131087296	25.400367540953145	29.339183856091307	27.209348471868257
4	12.897836801752463	16.99038882803943	36.548247535596936	33.563526834611174
5	14.504996101943442	36.82457796304777	33.45657860703483	15.213847327973953
6	32.28384685401418	36.10246511103168	17.202891084496237	14.410796950457902
7	28.005835423988984	31.413600328600943	22.07232051045261	18.508243736957464
8	27.179593555394653	32.799814633546255	20.148170875162922	19.87242093589617
9	27.016807393701846	14.42480932836587	19.610950015856034	38.94743326207625
10-11	25.39468442994894	24.81053659889845	27.27085735457104	22.52392161658158
12-13	26.40112382836066	22.56804617741672	26.77785801154326	24.252971982679362
14-15	24.21244971147708	23.589301160237802	24.375474618574692	27.82277450971043
16-17	24.11348456224506	26.14194887972776	25.223261851150735	24.52130470687645
18-19	24.411942915592835	25.483148212510976	26.82348036819471	23.28142850370148
20-21	25.267349213802255	24.948443917559548	26.069896513661668	23.714310354976533
22-23	24.755846493213017	24.757863583627632	25.787830108597504	24.69845981456185
24-25	24.42022291816324	25.254929218782372	25.78414307216681	24.540704790887574
26-27	24.59230640490311	25.040324253505542	25.941147607191816	24.42622173439953
28-29	24.304676264214393	24.961000775739237	26.34364618256777	24.3906767774786
30-31	24.735282938262955	24.934315714651735	26.096413698958166	24.23398764812714
32-33	24.2452892671533	25.126797884799256	25.789385443979402	24.83852740406804
34-35	24.610172824682056	25.07381684657426	26.171891797435308	24.144118531308376
36-37	24.808838800675936	24.981191889832427	25.819663169924663	24.390306139566974
38-39	24.807801759603247	24.699162463477414	25.923412332123707	24.56962344479563
40-41	24.623003812565926	24.988629409598957	25.99791054131223	24.39045623652289
42-43	25.057100199648108	24.891076024837247	25.67238854897948	24.37943522653516
44-45	24.686694909036806	25.147186795233324	25.456683215722226	24.709435080007648
46-47	24.467805583848424	25.073178829550546	25.809950494572604	24.64906509202843
48-49	25.033106184697356	25.238509720370306	25.303105817611815	24.425278277320526
50-51	24.336105845324806	25.565554425794435	25.171017925622568	24.92732180325819
52-53	24.380536755597873	25.395419642249323	24.985622846050475	25.238420756102332
54-55	24.56832282365528	25.237709384630037	25.091615863514278	25.102351928200413
56-57	24.67917282024589	24.884855369291405	24.96816594989484	25.467805860567864
58-59	24.398894472838716	24.89085077445669	25.50824062508441	25.20201412762018
60-61	24.515179781851206	25.14679617228513	25.240865630650923	25.097158415212746
62-63	24.088043151826678	25.193133881427748	25.135383298788838	25.583439667956736
64-65	24.062061607890737	24.91472499237797	25.36155699812627	25.661656401605022
66-67	24.58490430102753	24.87111992671522	25.204140058903963	25.33983571335329
68-69	24.539376515126282	24.62089504295624	25.210282303416285	25.629446138501194
70-71	24.07531783646626	24.68092703789344	25.414397210100354	25.82935791553994
72-73	24.582801566784898	24.761582807361446	25.26496996883066	25.390645657022997
74-75	24.666232721904983	24.660219061496658	25.24703916197863	25.426509054619732
76-77	24.340352668180852	24.805307575498535	25.41321707886512	25.441122677455493
78-79	24.347880998223186	24.893243907796318	25.35629546344897	25.402579630531523
80-81	24.020941587565904	24.850858511463276	25.54071375109437	25.587486149876447
82-83	24.1647181555427	24.887818595599143	25.218771190999533	25.72869205785862
84-85	24.18581323724987	25.00763237708428	24.96362443986839	25.84292994579746
86-87	24.06603163663819	24.745218304557522	25.178063488918717	26.01068656988557
88-89	24.088917227130995	24.449367438328622	25.35200554585117	26.109709788689216
90-91	24.29699446571689	24.611984420287392	25.18589097970121	25.905130134294506
92-93	24.076908307186432	24.705679653088648	25.13931427734985	26.07809776237507
94-95	23.575264551194017	24.741038285240847	25.509917926367986	26.17377923719715
96-97	24.139340632605517	24.60626816203813	25.179838027939912	26.074553177416444
98-99	23.908404400458114	24.613203199503555	25.173239520165723	26.305152879872608
100	23.87152334959745	24.655539219546803	25.117737440784506	26.35519999007124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	800.0
1	879.0
2	1260.5
3	1559.5
4	1301.0
5	1134.5
6	1524.5
7	2774.5
8	4496.0
9	4512.5
10	2973.5
11	2090.5
12	1952.5
13	2021.0
14	2299.5
15	2665.5
16	3032.0
17	3500.0
18	4165.5
19	4908.5
20	5861.0
21	7187.5
22	9156.0
23	12050.0
24	15290.0
25	19341.0
26	24911.0
27	31095.5
28	38015.5
29	48483.5
30	61388.0
31	73913.5
32	88071.0
33	102643.5
34	117893.0
35	132620.0
36	146924.5
37	166593.5
38	183648.5
39	196754.0
40	209028.5
41	222147.5
42	239330.5
43	268302.5
44	303636.5
45	335486.5
46	374987.0
47	414310.0
48	456099.0
49	490565.0
50	522042.5
51	524507.5
52	494333.5
53	485164.0
54	478509.5
55	460037.0
56	437523.5
57	404321.0
58	377342.5
59	348520.0
60	302696.0
61	254407.0
62	210862.5
63	168606.5
64	129696.0
65	102719.5
66	80179.5
67	61017.5
68	48315.5
69	39541.5
70	33766.0
71	29428.5
72	25141.0
73	23909.5
74	19786.0
75	14840.0
76	11202.5
77	7735.0
78	5510.0
79	3676.5
80	2445.5
81	1792.5
82	1273.5
83	844.0
84	548.5
85	352.0
86	227.0
87	136.0
88	85.5
89	51.5
90	31.5
91	27.0
92	17.5
93	12.5
94	9.0
95	5.0
96	2.0
97	2.5
98	3.0
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04921342474333826
2	0.02449264454127941
3	0.02129874529036742
4	0.021828020023375694
5	0.015056953611442263
6	0.02536868547867242
7	0.048264380394495834
8	0.005822022063091009
9	0.0038053028218008626
10-11	0.0018889632712536658
12-13	0.0012319325682089125
14-15	0.0022859193210098706
16-17	0.0067801918383646066
18-19	0.005005296397500655
20-21	0.008276761773077655
22-23	0.018401422398468682
24-25	0.025569444860158316
26-27	0.028621900001387063
28-29	0.04534424393651915
30-31	0.04260205329395043
32-33	0.04054883234693557
34-35	0.04808187186587229
36-37	0.04567732200125712
38-39	0.05560578595837784
40-41	0.05287728345545588
42-43	0.058151779932676256
44-45	0.05847116985776746
46-47	0.06357228323279547
48-49	0.06630534844893302
50-51	0.03436179322659748
52-53	3.102644986600224E-4
54-55	4.562713215588565E-6
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.566510123018049E-4
64-65	2.0988480791707395E-4
66-67	4.1520690261855937E-4
68-69	0.0013414376853830379
70-71	4.562713215588564E-5
72-73	2.5094922685737104E-4
74-75	7.300341144941704E-5
76-77	1.5969496254559975E-4
78-79	9.12542643117713E-6
80-81	5.475255858706277E-5
82-83	2.2813566077942824E-4
84-85	0.0012912478400115637
86-87	0.003946746931484109
88-89	0.0026509363782569557
90-91	0.003864618093603514
92-93	0.003960435071130874
94-95	0.002783255061509024
96-97	0.002441051570339882
98-99	0.004033438482580291
100	0.0029475127372702126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0958392E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.42876472656792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09620132983132	32.613352594122205
2	12.688307311957331	11.020750260587727
3	4.518975738410074	5.887606024454388
4	2.279267579400223	3.9594310181866494
5	1.2471077255244836	2.7080174000244024
6	0.8339491648436759	2.1730429244348284
7	0.556762174694324	1.6925645445416475
8	0.4173889767343635	1.4501350136047684
9	0.30913048808619265	1.208263971321393
>10	1.8053319363420701	14.544706931135904
>50	0.13698912021290385	4.132275467557889
>100	0.09096595338809325	7.959759590675786
>500	0.012064145152479826	3.616048126570103
>1k	0.007276003324612115	5.811306428523886
>5k	2.1719392153151483E-4	0.6624702243226451
>10k+	6.515817645945445E-5	0.5602694799355925
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	23355	0.21312433430014185	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	22752	0.20762170216214204	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13291	0.12128604269677522	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.12542643117713E-6	0.0
2	0.0	0.0	0.0	9.12542643117713E-6	0.0
3	0.0	0.0	0.0	9.12542643117713E-6	0.0
4	0.0	0.0	0.0	1.825085286235426E-5	0.0
5	0.0	9.12542643117713E-6	0.0	1.825085286235426E-5	0.0
6	9.12542643117713E-6	9.12542643117713E-6	0.0	1.825085286235426E-5	0.0
7	9.12542643117713E-6	9.12542643117713E-6	0.0	1.825085286235426E-5	0.0
8	9.12542643117713E-6	9.12542643117713E-6	0.0	1.825085286235426E-5	0.0
9	9.12542643117713E-6	9.12542643117713E-6	0.0	5.4752558587062774E-5	0.0
10-11	9.12542643117713E-6	9.12542643117713E-6	0.0	5.931527180265134E-5	0.0
12-13	9.12542643117713E-6	1.825085286235426E-5	0.0	8.212883788059415E-5	1.3688139646765694E-5
14-15	9.12542643117713E-6	2.7376279293531387E-5	0.0	8.212883788059415E-5	5.0189845371474215E-5
16-17	1.825085286235426E-5	3.650170572470852E-5	0.0	1.1863054360530268E-4	6.38779850182399E-5
18-19	2.281356607794282E-5	4.1064418940297084E-5	0.0	1.5056953611442263E-4	6.38779850182399E-5
20-21	3.650170572470852E-5	4.562713215588564E-5	0.0	2.2357294756383966E-4	6.38779850182399E-5
22-23	4.562713215588564E-5	5.018984537147421E-5	0.0	6.844069823382848E-4	6.38779850182399E-5
24-25	6.844069823382846E-5	5.4752558587062774E-5	0.0	0.001993905675212203	6.844069823382846E-5
26-27	9.125426431177129E-5	5.4752558587062774E-5	0.0	0.0035862925874526118	7.300341144941704E-5
28-29	9.125426431177129E-5	5.4752558587062774E-5	0.0	0.0124881460710659	7.300341144941704E-5
30-31	1.0950511717412555E-4	5.4752558587062774E-5	0.0	0.03750093991892241	7.300341144941704E-5
32-33	1.1863054360530268E-4	5.4752558587062774E-5	0.0	0.08481171325136024	8.212883788059415E-5
34-35	1.1863054360530268E-4	5.931527180265134E-5	0.0	0.14516272095395016	8.212883788059415E-5
36-37	1.1863054360530268E-4	6.38779850182399E-5	0.0	0.23862533846206632	8.212883788059415E-5
38-39	1.1863054360530268E-4	6.38779850182399E-5	0.0	0.3846686630666251	9.125426431177129E-5
40-41	1.2319325682089125E-4	6.38779850182399E-5	0.0	0.5663832795906554	9.125426431177129E-5
42-43	1.277559700364798E-4	6.38779850182399E-5	0.0	0.7299565483695053	9.125426431177129E-5
44-45	1.3688139646765693E-4	6.38779850182399E-5	0.0	0.9191996416992566	9.581697752735984E-5
46-47	1.4600682289883407E-4	6.38779850182399E-5	0.0	1.1353809938538428	1.0494240395853699E-4
48-49	1.4600682289883407E-4	6.38779850182399E-5	0.0	1.3606740842999594	1.0950511717412555E-4
50-51	1.551322493300112E-4	6.38779850182399E-5	0.0	1.6057647873885146	1.1863054360530268E-4
52-53	1.8707124183913116E-4	6.38779850182399E-5	0.0	1.8485604457296287	1.3688139646765693E-4
54-55	2.0075938148589683E-4	8.212883788059417E-5	0.0	2.0888739880814633	1.5969496254559975E-4
56-57	2.0075938148589683E-4	9.125426431177129E-5	0.0	2.366081629494546	1.642576757611883E-4
58-59	2.0075938148589683E-4	1.0037969074294842E-4	0.0	2.6665591083071316	1.642576757611883E-4
60-61	2.053220947014854E-4	1.0037969074294842E-4	0.0	2.9720190699511386	1.7338310219236545E-4
62-63	2.0988480791707395E-4	1.0037969074294842E-4	0.0	3.2795687542478857	1.9163395505471972E-4
64-65	2.0988480791707395E-4	1.0494240395853699E-4	0.0	3.611049869360395	1.9163395505471972E-4
66-67	2.0988480791707395E-4	1.1863054360530268E-4	0.0	3.9732517325534626	1.9619666827030828E-4
68-69	2.0988480791707395E-4	1.1863054360530268E-4	0.0	4.365275489323616	2.0075938148589683E-4
70-71	2.0988480791707395E-4	1.2319325682089125E-4	0.0	4.809277675045755	2.0075938148589683E-4
72-73	2.2813566077942821E-4	1.277559700364798E-4	0.0	5.237524812034467	2.0988480791707395E-4
74-75	2.326983739950168E-4	1.277559700364798E-4	0.0	5.666638864534139	2.0988480791707395E-4
76-77	2.3726108721060536E-4	1.277559700364798E-4	0.0	6.12395048470615	2.0988480791707395E-4
78-79	2.3726108721060536E-4	1.3688139646765693E-4	0.0	6.603414077539844	2.0988480791707395E-4
80-81	2.3726108721060536E-4	1.4600682289883407E-4	0.0	7.12488657094946	2.2357294756383966E-4
82-83	2.3726108721060536E-4	1.4600682289883407E-4	0.0	7.676326964759063	2.555119400729596E-4
84-85	2.3726108721060536E-4	1.551322493300112E-4	0.0	8.216269321265383	2.555119400729596E-4
86-87	2.3726108721060536E-4	1.642576757611883E-4	0.0	8.77507849691816	2.555119400729596E-4
88	2.3726108721060536E-4	1.642576757611883E-4	0.0	9.21474610508549	2.555119400729596E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	23345	0.0	18.862143	4
GTATAAG	3975	0.0	18.329344	1
GTACATG	73495	0.0	18.240808	1
GTATATA	2665	0.0	18.167383	1
GTACTAG	3000	0.0	18.018927	1
TACATGG	73400	0.0	17.646887	2
TAGTACT	4115	0.0	17.58718	4
CATGGGT	7705	0.0	17.382711	4
ACATGGG	68130	0.0	16.630682	3
CTAGTAC	4410	0.0	16.517502	3
GTACAAG	5565	0.0	15.626399	1
ATGGGTA	5495	0.0	15.392686	5
TATACCG	2195	0.0	15.199645	5
GTATCAA	52670	0.0	15.118282	1
GTATAGA	2835	0.0	15.088313	1
CCTAGTA	4655	0.0	15.0435915	2
GTATAAA	10945	0.0	14.859791	1
GTATATG	3735	0.0	14.598887	1
GTATTAA	3835	0.0	14.585925	1
TAGGCAT	4910	0.0	14.546942	5
>>END_MODULE