FastQCFastQC Report
Fri 27 May 2016
ERR765313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11503916
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT269880.23459837502290523No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA254900.22157672222224153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189610.16482213534938886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120280.10455570085873367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA28550.023.034961
GTACAAA50400.022.6485941
GTACTAG44350.021.8192161
GTACAAG58900.021.2144571
GTATAAG47750.020.5607261
TAGTACT46450.020.5280424
GTGATCG36500.019.430068
ATAAGGT43350.018.8540613
TAGGCAT65050.018.7735445
CTAGTAC56650.018.5734623
TGATCGC38650.018.4707349
CATGGGG287900.018.4526254
ATTTAGG56550.018.441061
CTATTGA53500.018.4355269
GTATCAA628700.018.3281921
GTACATG901350.017.5214391
CTAGGCA73100.017.4136224
GGGCTAT62800.017.1266146
TACATGA41700.017.1221372
TAAGGTG45600.017.0993824
Produced by FastQC (version 0.11.5)