##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765313_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11503916
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.656843634810965	33.0	33.0	33.0	27.0	33.0
2	31.546630816845326	33.0	33.0	33.0	27.0	33.0
3	31.552587744903562	33.0	33.0	33.0	27.0	33.0
4	35.28043015960826	37.0	37.0	37.0	33.0	37.0
5	35.34544176087517	37.0	37.0	37.0	33.0	37.0
6	35.36293971548471	37.0	37.0	37.0	33.0	37.0
7	35.17532421133812	37.0	37.0	37.0	33.0	37.0
8	35.15229318433827	37.0	37.0	37.0	33.0	37.0
9	35.00457948406439	37.0	37.0	37.0	33.0	37.0
10-11	34.98865812302523	37.0	37.0	37.0	33.0	37.0
12-13	34.9354672356787	37.0	37.0	37.0	33.0	37.0
14-15	36.45888752143183	37.0	37.0	40.0	33.0	40.0
16-17	36.5155048072326	37.0	37.0	40.0	33.0	40.0
18-19	36.47546057359946	37.0	37.0	40.0	33.0	40.0
20-21	36.200861384940566	37.0	37.0	40.0	27.0	40.0
22-23	36.24803453884746	37.0	37.0	40.0	27.0	40.0
24-25	36.147985520756585	37.0	37.0	40.0	27.0	40.0
26-27	36.11879220084708	37.0	37.0	40.0	27.0	40.0
28-29	36.04843515894935	37.0	37.0	40.0	27.0	40.0
30-31	36.14457972398269	37.0	37.0	40.0	27.0	40.0
32-33	35.915915415237734	37.0	37.0	40.0	27.0	40.0
34-35	35.7318592642714	37.0	37.0	40.0	27.0	40.0
36-37	35.81689869779995	37.0	37.0	40.0	27.0	40.0
38-39	35.728557127851076	37.0	37.0	40.0	27.0	40.0
40-41	35.7483523436715	37.0	37.0	40.0	27.0	40.0
42-43	35.62291401467118	37.0	37.0	40.0	27.0	40.0
44-45	35.521160316191455	37.0	35.0	40.0	27.0	40.0
46-47	35.304841759971126	37.0	33.0	40.0	27.0	40.0
48-49	35.043094108128045	37.0	33.0	40.0	27.0	40.0
50-51	34.83811951512859	37.0	33.0	40.0	27.0	40.0
52-53	34.68297373694314	37.0	33.0	40.0	27.0	40.0
54-55	34.542666601529426	37.0	33.0	40.0	27.0	40.0
56-57	34.37888771962521	37.0	33.0	40.0	27.0	40.0
58-59	34.193856726700716	37.0	33.0	38.5	27.0	40.0
60-61	33.9573590853758	37.0	33.0	37.0	27.0	40.0
62-63	33.71827723707301	37.0	33.0	37.0	27.0	40.0
64-65	33.52302168235582	37.0	33.0	37.0	22.0	40.0
66-67	33.24531281348021	37.0	33.0	37.0	22.0	40.0
68-69	33.04502775402741	37.0	33.0	37.0	22.0	40.0
70-71	32.76750708193627	37.0	33.0	37.0	22.0	37.0
72-73	32.551301356859696	37.0	33.0	37.0	22.0	37.0
74-75	32.35206463607697	35.0	33.0	37.0	22.0	37.0
76-77	32.089135212739734	33.0	33.0	37.0	22.0	37.0
78-79	32.011500301288706	33.0	33.0	37.0	22.0	37.0
80-81	31.851968668755926	33.0	33.0	37.0	22.0	37.0
82-83	31.69875423290643	33.0	33.0	37.0	22.0	37.0
84-85	31.53626199982684	33.0	33.0	37.0	22.0	37.0
86-87	31.36188011977834	33.0	33.0	37.0	22.0	37.0
88-89	31.222225935933466	33.0	33.0	37.0	18.5	37.0
90-91	31.08766132332677	33.0	27.0	37.0	15.0	37.0
92-93	30.961005191623443	33.0	27.0	37.0	15.0	37.0
94-95	30.810542862100174	33.0	27.0	37.0	15.0	37.0
96-97	30.674002183256555	33.0	27.0	37.0	15.0	37.0
98-99	30.548586933353825	33.0	27.0	37.0	15.0	37.0
100	30.335918047384908	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	28.0
7	296.0
8	1409.0
9	2885.0
10	4755.0
11	8974.0
12	17361.0
13	30895.0
14	42481.0
15	47455.0
16	52888.0
17	59018.0
18	66674.0
19	76699.0
20	87108.0
21	101186.0
22	116411.0
23	119641.0
24	117165.0
25	123859.0
26	138455.0
27	161335.0
28	196240.0
29	243517.0
30	303004.0
31	380670.0
32	492187.0
33	649749.0
34	869382.0
35	1225488.0
36	1968210.0
37	2826413.0
38	971375.0
39	701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56131605288465	18.632354075256355	12.838191890949865	24.968137980909134
2	16.196413929509553	20.855614136478483	37.14211622946567	25.805855704546293
3	18.163043283601034	26.080003574786144	29.054117845885337	26.70283529572749
4	13.125145778493888	17.4003644473214	35.92776671824498	33.54672305593974
5	14.340014347448033	37.187625443445434	32.83050318574728	15.641857023359249
6	31.7704577207915	36.6417952485832	17.179533899100722	14.408213131524574
7	27.571951375381808	31.96475662910817	21.88488649415668	18.57840550135334
8	27.329366198119782	32.4361682345605	19.76962379676069	20.46484177055903
9	27.33147956391332	14.457184811098417	19.324946400170795	38.88638922481747
10-11	25.508237485911266	25.18935360383815	26.40209289496738	22.9003160152832
12-13	26.579327780129898	23.37486783183711	26.279926985932185	23.765877402100806
14-15	24.526181998564738	23.88896705139571	24.24819584780191	27.336655102237646
16-17	23.821142151411635	26.277818863333746	25.128517623240548	24.772521362014068
18-19	24.245279492468832	25.344220426251766	27.19934228884525	23.211157792434147
20-21	25.592228158364723	24.536123440081898	26.327764664481997	23.54388373707139
22-23	25.011424804011305	24.390018922596536	26.118125670540255	24.480430602851904
24-25	24.33665730501324	25.06318061838211	26.262504889099336	24.337657187505314
26-27	24.541082733250747	24.93257562105749	26.174397147688467	24.3519444980033
28-29	24.089620752902984	24.804539158406335	26.803414220812893	24.30242586787779
30-31	24.599361221448866	24.82403676583217	26.343434754504525	24.23316725821444
32-33	24.194145520878465	24.964244028877825	25.999555210719468	24.842055239524242
34-35	24.509458915631033	25.01232845231995	26.519954351788215	23.9582582802608
36-37	24.610510897781268	24.963744212259662	25.9737928691291	24.451952020829975
38-39	24.677492567956772	24.561940259534815	26.095154243025192	24.665412929483217
40-41	24.420299380110226	24.893081482613297	26.312227855484366	24.374391281792107
42-43	24.946085184755812	24.82652766325533	25.842623095674455	24.384764056314403
44-45	24.465554869071067	25.21345432872434	25.599905139622724	24.721085662581864
46-47	24.24396472794923	25.08812707761966	25.912282880483563	24.755625313947547
48-49	24.896540412269445	25.209382550745314	25.347096752423436	24.546980284561805
50-51	24.158738384899976	25.583891866830694	25.053310953871993	25.204058794397334
52-53	24.1259648066365	25.441347473866934	24.859136328903276	25.57355139059329
54-55	24.34835442201463	25.29055295046256	24.962756867833562	25.398335759689246
56-57	24.603024344976745	24.814007970270318	24.790058642162215	25.792909042590722
58-59	24.161482004831935	24.836969747925316	25.492042572582296	25.509505674660453
60-61	24.231299068144335	25.103918217614762	25.19220349051138	25.472579223729525
62-63	23.841110791583333	24.98878935707316	25.046478249613795	26.123621601729713
64-65	23.751169529002993	24.8026272400385	25.27007799961229	26.176125231346216
66-67	24.4505569990378	24.68689835382738	25.098771922288815	25.763772724846007
68-69	24.28554597151453	24.31278802845069	25.2064153174617	26.195250682573086
70-71	23.682721699608418	24.511006120257342	25.390731431466683	26.415540748667553
72-73	24.324959416853034	24.516350924805348	25.311728000160684	25.846961658180934
74-75	24.502524627765922	24.427307687843353	25.313547739433602	25.756619944957126
76-77	24.037479838446725	24.63391401505106	25.42216010120991	25.906446045292302
78-79	24.072347877137886	24.675241056627293	25.348363666021456	25.904047400213365
80-81	23.7187005192892	24.584885352890108	25.695379175193274	26.00103495262742
82-83	23.967884593496454	24.56873116267924	25.33531876509518	26.128065478729123
84-85	24.06823766309911	24.71314320236711	24.893907699938065	26.32471143459571
86-87	23.801590278386758	24.470598127607566	25.27100239484776	26.45680919915792
88-89	23.92605722364511	24.025385080222048	25.393584349136678	26.65497334699617
90-91	24.086224033221267	24.21863746939385	25.241876470907155	26.45326202647773
92-93	23.885917941564372	24.34144548473736	25.207087815877344	26.565548757820928
94-95	23.293875065014646	24.445528281517337	25.626502488827786	26.63409416464023
96-97	23.93283043478261	24.21694347826087	25.186360869565217	26.663865217391304
98-99	23.60495062380627	24.360325148221627	25.163639117804642	26.871085110167463
100	23.634664661839835	24.35486304577432	25.08265345034727	26.927818842038576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1612.0
1	1382.5
2	1420.0
3	1654.5
4	1341.5
5	1115.0
6	1449.5
7	2687.5
8	4367.5
9	4388.5
10	2898.5
11	2038.5
12	1959.0
13	2071.5
14	2404.5
15	2862.0
16	3390.0
17	4107.0
18	4907.0
19	5899.5
20	7279.5
21	9143.5
22	11464.0
23	14104.0
24	17233.5
25	21267.0
26	27055.0
27	33691.5
28	40794.0
29	51875.0
30	65450.5
31	77725.0
32	93294.5
33	109247.5
34	124645.5
35	138585.0
36	151229.0
37	171908.0
38	186107.5
39	193343.0
40	199582.0
41	205189.5
42	219882.0
43	250756.5
44	291222.0
45	334230.5
46	385703.0
47	436055.0
48	485187.5
49	521453.0
50	560268.5
51	571290.5
52	542826.5
53	533884.5
54	527741.0
55	507886.0
56	485508.5
57	450023.0
58	417308.0
59	386434.0
60	333859.0
61	270796.0
62	215756.5
63	170972.5
64	130091.0
65	100647.0
66	78448.0
67	57786.0
68	43395.0
69	34407.0
70	28861.0
71	24921.5
72	21589.5
73	21375.0
74	17170.0
75	12227.0
76	9000.0
77	6263.5
78	4647.5
79	2959.5
80	1950.0
81	1531.5
82	1196.5
83	920.5
84	668.0
85	471.0
86	343.5
87	195.0
88	109.0
89	76.0
90	74.5
91	60.0
92	35.0
93	26.0
94	26.0
95	15.5
96	6.5
97	5.5
98	3.5
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017185452327711712
2	0.006432592171222391
3	0.009822742099299056
4	0.008857853273615698
5	0.019289083821543897
6	0.004207262987664375
7	0.023870132570509035
8	0.007866886371562519
9	0.0012865184342444782
10-11	3.6509306917748707E-4
12-13	9.170790190053544E-4
14-15	9.170790190053544E-4
16-17	0.0012474013196897473
18-19	0.003355379159583571
20-21	0.006502133708208578
22-23	0.013304165294670094
24-25	0.022292408950134894
26-27	0.026673525780264738
28-29	0.03235854642888561
30-31	0.034766422147032364
32-33	0.03554007174600371
34-35	0.04440661771174268
36-37	0.04100777509154274
38-39	0.04544104807441223
40-41	0.042207366604554486
42-43	0.049417954720809856
44-45	0.04268546467133453
46-47	0.04502814519855674
48-49	0.04750556245368968
50-51	0.0484661049333114
52-53	0.033714606400116275
54-55	0.030194066090190503
56-57	0.022162018568285792
58-59	0.021931662227019044
60-61	0.022288062604073254
62-63	0.022327179718627986
64-65	0.026299740018963978
66-67	0.027625375567763188
68-69	0.02882496708077493
70-71	0.02901185996142531
72-73	0.027334170381633525
74-75	0.02188385242034104
76-77	0.026651794049956556
78-79	0.026421437708689807
80-81	0.02526530965629443
82-83	0.027321131343448615
84-85	0.0288336597728982
86-87	0.031315423374092786
88-89	0.031445813755941895
90-91	0.031054642610394582
92-93	0.033406015829740066
94-95	0.031489277216558254
96-97	0.03404058235473903
98-99	0.03469688061004618
100	0.043472153308490774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1503916E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.34290695328815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6558242345599	24.265263973953726
2	14.107175106043869	9.68962804081215
3	5.77726485487221	5.952242080661342
4	2.8202138000979806	3.874173605005766
5	1.5892233261665907	2.7289274409267166
6	1.0285363897456692	2.1193757718663857
7	0.6841483805458387	1.6446950922729957
8	0.5149175342506764	1.4147011973910038
9	0.38402401672620357	1.1869650966830383
>10	2.0751667962992695	13.129264289915197
>50	0.17720288871295523	4.232722178110039
>100	0.14294417863365685	10.178811526503598
>500	0.024686361128432036	5.905111383365006
>1k	0.017855720459423158	11.244767691779685
>5k	7.110683047323163E-4	1.6784332680475862
>10k+	1.0534345255293574E-4	0.7549173627057949
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	26988	0.23459837502290523	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	25490	0.22157672222224153	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18961	0.16482213534938886	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12028	0.10455570085873367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.692692123273501E-6	0.0
5	0.0	0.0	0.0	8.692692123273501E-6	0.0
6	0.0	0.0	0.0	8.692692123273501E-6	0.0
7	0.0	0.0	0.0	8.692692123273501E-6	8.692692123273501E-6
8	0.0	0.0	0.0	8.692692123273501E-6	8.692692123273501E-6
9	0.0	8.692692123273501E-6	0.0	8.692692123273501E-6	8.692692123273501E-6
10-11	8.692692123273501E-6	8.692692123273501E-6	0.0	2.6078076369820505E-5	8.692692123273501E-6
12-13	1.3039038184910253E-5	8.692692123273501E-6	0.0	4.3463460616367504E-5	8.692692123273501E-6
14-15	1.7385384246547002E-5	8.692692123273501E-6	0.0	4.3463460616367504E-5	3.4770768493094005E-5
16-17	2.1731730308183752E-5	8.692692123273501E-6	0.0	4.3463460616367504E-5	3.911711455473076E-5
18-19	2.6078076369820502E-5	8.692692123273501E-6	0.0	6.084884486291451E-5	4.3463460616367504E-5
20-21	4.780980667800425E-5	8.692692123273501E-6	0.0	2.43395379451658E-4	6.084884486291451E-5
22-23	5.2156152739641003E-5	8.692692123273501E-6	0.0	0.0015603382361275933	6.084884486291451E-5
24-25	6.08488448629145E-5	8.692692123273501E-6	0.0	0.00417249221917128	6.084884486291451E-5
26-27	8.692692123273501E-5	8.692692123273501E-6	0.0	0.007201895424132096	6.084884486291451E-5
28-29	9.996595941764526E-5	8.692692123273501E-6	0.0	0.02068860725339093	6.084884486291451E-5
30-31	1.2169768972582902E-4	1.3039038184910253E-5	0.0	0.062491763674213205	6.084884486291451E-5
32-33	1.3039038184910253E-4	1.7385384246547002E-5	0.0	0.13713591093676275	6.519519092455126E-5
34-35	1.3039038184910253E-4	1.7385384246547002E-5	0.0	0.23526336597033565	6.954153698618801E-5
36-37	1.3039038184910253E-4	1.7385384246547002E-5	0.0	0.38788096157864854	6.954153698618801E-5
38-39	1.3039038184910253E-4	1.7385384246547002E-5	0.0	0.6150210067597851	6.954153698618801E-5
40-41	1.3039038184910253E-4	1.7385384246547002E-5	0.0	0.8857722883233848	6.954153698618801E-5
42-43	1.4342942003401276E-4	1.7385384246547002E-5	0.0	1.1333879698008922	6.954153698618801E-5
44-45	1.6516115034219652E-4	2.1731730308183752E-5	0.0	1.4265881287728457	7.823422910946152E-5
46-47	1.6950749640383327E-4	2.6078076369820502E-5	0.0	1.746965989668214	7.823422910946152E-5
48-49	1.7385384246547002E-4	2.6078076369820502E-5	0.0	2.0811217675789706	7.823422910946152E-5
50-51	1.7385384246547002E-4	4.3463460616367504E-5	0.0	2.442802955098073	7.823422910946152E-5
52-53	1.9123922671201703E-4	4.3463460616367504E-5	0.0	2.80444502550262	8.692692123273501E-5
54-55	1.9558557277365377E-4	4.3463460616367504E-5	0.0	3.16566984668525	8.692692123273501E-5
56-57	1.9993191883529052E-4	4.3463460616367504E-5	0.0	3.5800852509701913	8.692692123273501E-5
58-59	2.0427826489692727E-4	4.3463460616367504E-5	0.0	4.026133361891724	9.127326729437175E-5
60-61	2.0862461095856401E-4	4.3463460616367504E-5	0.0	4.469204225761036	9.996595941764526E-5
62-63	2.1731730308183753E-4	4.3463460616367504E-5	0.0	4.91466992631031	1.0431230547928201E-4
64-65	2.1731730308183753E-4	4.3463460616367504E-5	0.0	5.386178932460911	1.0431230547928201E-4
66-67	2.2166364914347428E-4	4.3463460616367504E-5	0.0	5.8901334119616315	1.0431230547928201E-4
68-69	2.3035634126674777E-4	5.2156152739641003E-5	0.0	6.4330659229431095	1.0431230547928201E-4
70-71	2.3470268732838452E-4	6.084884486291451E-5	0.0	7.042471450591259	1.0431230547928201E-4
72-73	2.390490333900213E-4	6.954153698618801E-5	0.0	7.632209762310504	1.0431230547928201E-4
74-75	2.4339537945165804E-4	9.127326729437175E-5	0.0	8.218744816982323	1.0431230547928201E-4
76-77	2.4339537945165804E-4	1.3039038184910253E-4	0.0	8.850355826659374	1.0865865154091877E-4
78-79	2.4339537945165804E-4	1.3039038184910253E-4	0.0	9.508149225011728	1.1300499760255551E-4
80-81	2.4339537945165804E-4	1.3039038184910253E-4	0.0	10.21132716893969	1.1300499760255551E-4
82-83	2.4339537945165804E-4	1.3039038184910253E-4	0.0	10.948715202718796	1.1300499760255551E-4
84-85	2.4339537945165804E-4	1.3908307397237602E-4	0.0	11.670260805103236	1.1300499760255551E-4
86-87	2.4339537945165804E-4	1.3908307397237602E-4	0.0	12.412429819550143	1.1300499760255551E-4
88	2.4339537945165804E-4	1.477757660956495E-4	0.0	12.985160879130202	1.1300499760255551E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	2855	0.0	23.03496	1
GTACAAA	5040	0.0	22.648594	1
GTACTAG	4435	0.0	21.819216	1
GTACAAG	5890	0.0	21.214457	1
GTATAAG	4775	0.0	20.560726	1
TAGTACT	4645	0.0	20.528042	4
GTGATCG	3650	0.0	19.43006	8
ATAAGGT	4335	0.0	18.854061	3
TAGGCAT	6505	0.0	18.773544	5
CTAGTAC	5665	0.0	18.573462	3
TGATCGC	3865	0.0	18.470734	9
CATGGGG	28790	0.0	18.452625	4
ATTTAGG	5655	0.0	18.44106	1
CTATTGA	5350	0.0	18.435526	9
GTATCAA	62870	0.0	18.328192	1
GTACATG	90135	0.0	17.521439	1
CTAGGCA	7310	0.0	17.413622	4
GGGCTAT	6280	0.0	17.126614	6
TACATGA	4170	0.0	17.122137	2
TAAGGTG	4560	0.0	17.099382	4
>>END_MODULE