##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765312_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5840146 Sequences flagged as poor quality 0 Sequence length 99 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.274695701100626 33.0 33.0 33.0 33.0 33.0 2 32.103359744773506 33.0 33.0 33.0 27.0 33.0 3 32.156006031356064 33.0 33.0 33.0 27.0 33.0 4 36.06146747016256 37.0 37.0 37.0 33.0 37.0 5 36.09351170330331 37.0 37.0 37.0 33.0 37.0 6 36.13699811614298 37.0 37.0 37.0 33.0 37.0 7 36.04340679154254 37.0 37.0 37.0 33.0 37.0 8 36.016649926217596 37.0 37.0 37.0 33.0 37.0 9 35.719288010950415 37.0 37.0 37.0 33.0 37.0 10-11 35.90014684564393 37.0 37.0 37.0 33.0 37.0 12-13 35.82943508604066 37.0 37.0 37.0 33.0 37.0 14-15 37.13014220877355 37.0 37.0 40.0 33.0 40.0 16-17 37.292165897907346 37.0 37.0 40.0 33.0 40.0 18-19 37.41611648064963 37.0 37.0 40.0 33.0 40.0 20-21 37.46330939329256 37.0 37.0 40.0 33.0 40.0 22-23 37.40488910722438 37.0 37.0 40.0 33.0 40.0 24-25 37.34631300313383 37.0 37.0 40.0 33.0 40.0 26-27 37.18105771670777 37.0 37.0 40.0 33.0 40.0 28-29 37.10287063028904 37.0 37.0 40.0 33.0 40.0 30-31 36.90103620697154 37.0 37.0 40.0 33.0 40.0 32-33 36.62239599831922 37.0 37.0 40.0 33.0 40.0 34-35 36.50362131357675 37.0 37.0 40.0 33.0 40.0 36-37 36.31532114094408 37.0 37.0 40.0 33.0 40.0 38-39 36.117055977710145 37.0 37.0 40.0 33.0 40.0 40-41 35.89572974716728 37.0 37.0 40.0 33.0 40.0 42-43 35.64399725623298 37.0 37.0 40.0 27.0 40.0 44-45 35.275756205409934 37.0 35.0 40.0 27.0 40.0 46-47 34.83265854997461 37.0 33.0 40.0 22.0 40.0 48-49 34.42665756986212 37.0 33.0 40.0 22.0 40.0 50-51 34.07617472234427 37.0 33.0 40.0 22.0 40.0 52-53 33.926329153415 37.0 33.0 40.0 22.0 40.0 54-55 33.83399593092364 37.0 33.0 40.0 22.0 40.0 56-57 33.66407192559912 37.0 33.0 40.0 22.0 40.0 58-59 33.4690060830671 37.0 33.0 40.0 18.5 40.0 60-61 33.27157291958112 37.0 33.0 37.0 15.0 40.0 62-63 32.92361552262563 37.0 33.0 37.0 15.0 40.0 64-65 32.80861154840992 37.0 33.0 37.0 15.0 40.0 66-67 32.6490187060392 37.0 33.0 37.0 15.0 40.0 68-69 32.41299789423073 37.0 33.0 37.0 15.0 40.0 70-71 32.20203304848886 37.0 33.0 37.0 15.0 38.5 72-73 31.975153018434817 37.0 33.0 37.0 15.0 37.0 74-75 31.76094895572816 35.0 33.0 37.0 15.0 37.0 76-77 31.566942590133877 33.0 33.0 37.0 15.0 37.0 78-79 31.36201774750152 33.0 33.0 37.0 15.0 37.0 80-81 31.239809244494914 33.0 33.0 37.0 15.0 37.0 82-83 31.0742225451213 33.0 33.0 37.0 15.0 37.0 84-85 30.904770360193048 33.0 33.0 37.0 15.0 37.0 86-87 30.789384460593965 33.0 27.0 37.0 15.0 37.0 88-89 30.67411533889735 33.0 27.0 37.0 15.0 37.0 90-91 30.590945157877904 33.0 27.0 37.0 15.0 37.0 92-93 30.438726103765212 33.0 27.0 37.0 15.0 37.0 94-95 30.3911171912483 33.0 27.0 37.0 15.0 37.0 96-97 30.35226816247402 33.0 27.0 37.0 15.0 37.0 98-99 29.551880124229772 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18985326885233178 1101 2 0.37894889253001196 1101 3 0.3112546410988202 1101 4 0.28355621260401165 1101 5 0.12914854368361972 1101 6 0.19518151077207335 1101 7 0.21163652669817168 1101 8 0.22514209221783688 1101 9 0.4344579478461341 1101 10-11 0.36757078056918857 1101 12-13 0.3623445118484483 1101 14-15 0.648136718196298 1101 16-17 0.544992814881553 1101 18-19 0.37479829697147693 1101 20-21 0.43641578405087245 1101 22-23 0.335874526707272 1101 24-25 0.47918050810399393 1101 26-27 0.38440162313657567 1101 28-29 0.4274413996826496 1101 30-31 0.3778140786942288 1101 32-33 0.41168129273795984 1101 34-35 0.45972322788078657 1101 36-37 0.48963464718086414 1101 38-39 0.2891773584631423 1101 40-41 0.2970774716031812 1101 42-43 0.4437221665509625 1101 44-45 0.5541763276678253 1101 46-47 0.528300001718101 1101 48-49 0.5126065305341996 1101 50-51 0.5667670949358978 1101 52-53 0.5705031005654178 1101 54-55 0.5640263500009794 1101 56-57 0.5878020131836976 1101 58-59 0.49478341857277286 1101 60-61 0.4486924432058572 1101 62-63 0.4622713749523939 1101 64-65 0.34144558804308645 1101 66-67 0.45645147247031304 1101 68-69 0.28088361082745905 1101 70-71 0.376461143094005 1101 72-73 0.46046274454243985 1101 74-75 0.3436993420474721 1101 76-77 0.37458068592768967 1101 78-79 0.6083098625396381 1101 80-81 0.624935391470995 1101 82-83 0.5904954850332373 1101 84-85 0.5769093406362096 1101 86-87 0.5045569250158444 1101 88-89 0.5905069829963665 1101 90-91 0.5324856422516078 1101 92-93 0.6905084434300974 1101 94-95 0.6269970173580219 1101 96-97 0.49778697095857183 1101 98-99 0.5336224215623844 1102 1 0.10707862850158278 1102 2 0.33658405345943976 1102 3 0.2598879431445056 1102 4 0.21340081802566147 1102 5 0.06815111979253885 1102 6 0.18087159285196464 1102 7 0.21938199160195637 1102 8 0.15900525900433848 1102 9 0.44177147980281717 1102 10-11 0.3217703607540656 1102 12-13 0.3341788627799005 1102 14-15 0.6183217798159433 1102 16-17 0.3838332138278133 1102 18-19 0.2679478790879486 1102 20-21 0.3413563689164363 1102 22-23 0.22315282958395954 1102 24-25 0.3970718137756961 1102 26-27 0.30228794199293674 1102 28-29 0.32398619768814285 1102 30-31 0.29905165988105864 1102 32-33 0.3623150486740414 1102 34-35 0.38689032580604277 1102 36-37 0.349847467192987 1102 38-39 0.24859398508279895 1102 40-41 0.30959107291654675 1102 42-43 0.26746435061990326 1102 44-45 0.37987717044477165 1102 46-47 0.479333398716868 1102 48-49 0.5626385015615014 1102 50-51 0.45918785463327083 1102 52-53 0.5844584971488018 1102 54-55 0.534478747446606 1102 56-57 0.6546756773582487 1102 58-59 0.5647063592609314 1102 60-61 0.42383223446420715 1102 62-63 0.5444313162177608 1102 64-65 0.4918912740341739 1102 66-67 0.5974829595316891 1102 68-69 0.578767372620149 1102 70-71 0.5506204955285838 1102 72-73 0.5643101267053439 1102 74-75 0.45477937182610617 1102 76-77 0.4049043761174431 1102 78-79 0.6579608962832637 1102 80-81 0.5891335235465576 1102 82-83 0.5669235562523838 1102 84-85 0.5618571797073777 1102 86-87 0.4957328841125168 1102 88-89 0.5619074616636333 1102 90-91 0.4282122932798629 1102 92-93 0.4944429196143716 1102 94-95 0.5286640092200265 1102 96-97 0.35325546757085036 1102 98-99 0.36819992452038974 1103 1 0.10266732657689914 1103 2 0.28766367643133606 1103 3 0.28081779117952976 1103 4 0.1933526615341279 1103 5 0.11706531320939462 1103 6 0.19105680051616503 1103 7 0.1657005132521121 1103 8 0.10164952201996158 1103 9 0.46425341426192546 1103 10-11 0.33830269619052444 1103 12-13 0.30968066473754163 1103 14-15 0.5041548445579167 1103 16-17 0.42797119607203626 1103 18-19 0.24100963693397404 1103 20-21 0.334211555292768 1103 22-23 0.27363286065895664 1103 24-25 0.3384027514061927 1103 26-27 0.2227259999337008 1103 28-29 0.12725466286555331 1103 30-31 0.16366443097082595 1103 32-33 0.2466043945480223 1103 34-35 0.3950823409786679 1103 36-37 0.3400858432481755 1103 38-39 0.24180825897040847 1103 40-41 0.13285287376088917 1103 42-43 0.201808048786134 1103 44-45 0.2703815217770966 1103 46-47 0.41424789372127435 1103 48-49 0.3710518963656 1103 50-51 0.3717706185241525 1103 52-53 0.31523761769147995 1103 54-55 0.31790550875494006 1103 56-57 0.35731155148624083 1103 58-59 0.28578211766625117 1103 60-61 0.26420105932209026 1103 62-63 0.35491324961028425 1103 64-65 0.08781070896942822 1103 66-67 0.18569030752075122 1103 68-69 0.23574610866664614 1103 70-71 0.3008332460384864 1103 72-73 0.24878147136830364 1103 74-75 0.24485484210306652 1103 76-77 0.1825870831648082 1103 78-79 0.37493150500760564 1103 80-81 0.27807768246419684 1103 82-83 0.2820276349369699 1103 84-85 0.32383545052283225 1103 86-87 0.24060861308029047 1103 88-89 0.2588411320084383 1103 90-91 0.11362143909230937 1103 92-93 0.18417039097904464 1103 94-95 0.29612301022677556 1103 96-97 0.19338212849898895 1103 98-99 0.19033733660264218 1104 1 0.14621314039650457 1104 2 0.32482447988220997 1104 3 0.21578870641338455 1104 4 0.17459757854170022 1104 5 0.13891880995232242 1104 6 0.18354071312393216 1104 7 0.16256928215372568 1104 8 0.08117023135785217 1104 9 0.4107802086052388 1104 10-11 0.24219723699776097 1104 12-13 0.28071741926498106 1104 14-15 0.400107035834516 1104 16-17 0.3298686527739392 1104 18-19 0.20674582590536517 1104 20-21 0.30176660097441044 1104 22-23 0.23411523532475087 1104 24-25 0.3306733700484159 1104 26-27 0.21564630557936226 1104 28-29 0.15281153905100098 1104 30-31 0.1612013693455907 1104 32-33 0.22343138730409606 1104 34-35 0.26031372295415167 1104 36-37 0.15677212263454976 1104 38-39 0.02664498896816525 1104 40-41 0.08580472591236088 1104 42-43 0.1146292214814224 1104 44-45 0.15447970231014807 1104 46-47 0.26783316454370976 1104 48-49 0.1650751906930239 1104 50-51 0.1430931045148185 1104 52-53 0.14521528009187534 1104 54-55 0.15333613250559353 1104 56-57 0.23252094847003235 1104 58-59 0.22884332990174272 1104 60-61 0.14538572598454635 1104 62-63 0.18588889019138577 1104 64-65 -0.06115732640743943 1104 66-67 -0.03821863532809289 1104 68-69 -0.014339643727510065 1104 70-71 -0.005163455786721727 1104 72-73 0.057681881957307723 1104 74-75 0.06220910300527294 1104 76-77 0.08286150335657894 1104 78-79 0.20624352653718958 1104 80-81 0.1321376144346722 1104 82-83 0.06831864709866053 1104 84-85 0.12285596608925786 1104 86-87 0.14591972554803334 1104 88-89 0.017760759353731004 1104 90-91 -0.001295465227592274 1104 92-93 0.11041985479946703 1104 94-95 0.15367884182062497 1104 96-97 0.047059116227831765 1104 98-99 0.028566143612977157 1105 1 0.13623856599460282 1105 2 0.288388019489382 1105 3 0.19175106257507935 1105 4 0.20517044290984643 1105 5 0.011546863980164801 1105 6 0.061860078027017096 1105 7 0.12532678272010855 1105 8 -0.006101066922234111 1105 9 0.3566875268884786 1105 10-11 0.20645852585799673 1105 12-13 0.2356601842186663 1105 14-15 0.43336290620915463 1105 16-17 0.3352303324484467 1105 18-19 0.20942885058571648 1105 20-21 0.27711593124343636 1105 22-23 0.161394021080838 1105 24-25 0.29838999761572893 1105 26-27 0.13328645990323906 1105 28-29 0.11698186747736372 1105 30-31 0.10877069964598718 1105 32-33 0.19305220834348802 1105 34-35 0.19729971060295526 1105 36-37 0.1519472607782575 1105 38-39 0.16790002559174155 1105 40-41 0.11399082659037418 1105 42-43 0.18918387389379632 1105 44-45 0.16595879328693997 1105 46-47 0.34422315985955976 1105 48-49 0.2852029065370374 1105 50-51 0.1581554315527427 1105 52-53 0.15806064496403138 1105 54-55 0.21146556395563465 1105 56-57 0.2294384523050894 1105 58-59 0.24345711051765306 1105 60-61 0.14128236654738657 1105 62-63 0.2592589281419251 1105 64-65 0.12769670209590345 1105 66-67 0.19182633359820755 1105 68-69 0.08689554705112812 1105 70-71 0.1559964912362446 1105 72-73 0.2916863654794497 1105 74-75 0.2052501648345384 1105 76-77 0.2046710068489297 1105 78-79 0.3422578528335336 1105 80-81 0.28360294575511347 1105 82-83 0.22895057169744604 1105 84-85 0.1659295316613303 1105 86-87 0.12067685129244055 1105 88-89 0.14642878413381055 1105 90-91 0.14776997467021147 1105 92-93 0.15559423104916803 1105 94-95 0.24561562045359508 1105 96-97 0.22016014583794785 1105 98-99 0.10677488075947394 1106 1 0.09749069357982876 1106 2 0.2770035638239037 1106 3 0.19663495947930443 1106 4 0.15603462041077876 1106 5 0.08774104899183044 1106 6 0.10661461538555983 1106 7 0.13629853967736238 1106 8 0.0763439594081845 1106 9 0.36459596008293005 1106 10-11 0.2132432305612113 1106 12-13 0.20749050520606005 1106 14-15 0.40847660522043583 1106 16-17 0.3292190868888767 1106 18-19 0.23092438766218493 1106 20-21 0.35448467480564005 1106 22-23 0.1620390013391173 1106 24-25 0.2609356689411726 1106 26-27 0.11241580748021818 1106 28-29 0.15618196488193803 1106 30-31 0.12310204277562775 1106 32-33 0.14993682957606325 1106 34-35 0.24678321661811964 1106 36-37 0.14239556860273694 1106 38-39 0.05412872184947304 1106 40-41 0.0033572077369186104 1106 42-43 0.10599320457865247 1106 44-45 0.1106841698541956 1106 46-47 0.2216605831065408 1106 48-49 0.3057463035128549 1106 50-51 0.20469888408699433 1106 52-53 0.21425648934071262 1106 54-55 0.20609250161152914 1106 56-57 0.24928984382967911 1106 58-59 0.20541254733182512 1106 60-61 0.19987195208882724 1106 62-63 0.25020866563569655 1106 64-65 0.11471132120514227 1106 66-67 0.19768719458552653 1106 68-69 0.12842858163965332 1106 70-71 0.08640840385168502 1106 72-73 0.2012473888126607 1106 74-75 0.2606629455505569 1106 76-77 0.18865314747117523 1106 78-79 0.21837698223873758 1106 80-81 0.22802585391716335 1106 82-83 0.2134453718858147 1106 84-85 0.14483125218495374 1106 86-87 0.1317060506495551 1106 88-89 0.12987879757274712 1106 90-91 0.08092327502606622 1106 92-93 0.010244716025560763 1106 94-95 0.27056590230183986 1106 96-97 0.2769351425908795 1106 98-99 0.025485499469162676 1107 1 0.09997109238655355 1107 2 0.28160519656759675 1107 3 0.20596847553930075 1107 4 0.12784296095777137 1107 5 0.02292440305539145 1107 6 0.08049771311136311 1107 7 0.08534454458438034 1107 8 0.02076397283511966 1107 9 0.3363670035736135 1107 10-11 0.2323101579603204 1107 12-13 0.15581695159457354 1107 14-15 0.2926010120093139 1107 16-17 0.23173567685081764 1107 18-19 0.1306290614748562 1107 20-21 0.20450330887756962 1107 22-23 0.10051067639328437 1107 24-25 0.18517197557270748 1107 26-27 0.02327490324923076 1107 28-29 0.12619968532055026 1107 30-31 0.08647738155723061 1107 32-33 -0.0232483289132972 1107 34-35 0.12531993166687272 1107 36-37 0.058600214370329695 1107 38-39 -0.0041771663437728535 1107 40-41 0.03929725835683229 1107 42-43 0.09086176715009486 1107 44-45 0.0768922417467337 1107 46-47 0.04004177232173589 1107 48-49 0.21026190004312895 1107 50-51 0.08958939843364533 1107 52-53 0.1168826936164038 1107 54-55 0.1482913524574272 1107 56-57 0.21287747225154163 1107 58-59 0.13707010079527748 1107 60-61 0.16184933792948186 1107 62-63 0.181313015571142 1107 64-65 0.02174504728515103 1107 66-67 0.010019393972704904 1107 68-69 0.03260369796888085 1107 70-71 0.07962608595239118 1107 72-73 0.21368357181457043 1107 74-75 0.14196340138419572 1107 76-77 0.18259476109795614 1107 78-79 0.23999015654204925 1107 80-81 0.1724324230563674 1107 82-83 0.09630793107681868 1107 84-85 0.06482003126437164 1107 86-87 0.10098466052755128 1107 88-89 0.0958096713820602 1107 90-91 0.012626546295944507 1107 92-93 0.01952420195069493 1107 94-95 0.16889842753228734 1107 96-97 0.10225278918710501 1107 98-99 0.21849650094427275 1108 1 0.08722480500292562 1108 2 0.3016551673730845 1108 3 0.15619532353319698 1108 4 0.1284955386728157 1108 5 -0.009042740360541757 1108 6 0.06108487243783145 1108 7 0.0269163054576822 1108 8 -0.025833307256483806 1108 9 0.2772164600265441 1108 10-11 0.16609409304079037 1108 12-13 0.17023096382792602 1108 14-15 0.2223451679099071 1108 16-17 0.23108165830616656 1108 18-19 -0.01723695176377049 1108 20-21 0.19024750446003225 1108 22-23 0.05293020724026576 1108 24-25 0.06258737289210359 1108 26-27 -0.0821911791492198 1108 28-29 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-0.4811706157150937 2216 6 -0.3806408113776669 2216 7 -0.3896706773129779 2216 8 -0.4064119310694281 2216 9 -0.09100497243500172 2216 10-11 -0.4346741788701891 2216 12-13 -0.3206114607038657 2216 14-15 -0.7652354234675869 2216 16-17 -0.9417369711035875 2216 18-19 -0.8658316469993608 2216 20-21 -1.1907801343833597 2216 22-23 -1.072433898162572 2216 24-25 -0.8862899714421673 2216 26-27 -0.998487872103702 2216 28-29 -1.1584626848770228 2216 30-31 -0.7632211785914222 2216 32-33 -0.8968045822404207 2216 34-35 -0.9180265219232311 2216 36-37 -0.8849384147042798 2216 38-39 -0.9766592558564966 2216 40-41 -0.8431879357816996 2216 42-43 -0.9483921305295837 2216 44-45 -0.8344515314241931 2216 46-47 -1.041856185477279 2216 48-49 -1.2245294839440817 2216 50-51 -0.912267546345646 2216 52-53 -0.8677237457400508 2216 54-55 -0.8548174039784655 2216 56-57 -0.9815527315610524 2216 58-59 -0.7836137686063793 2216 60-61 -0.86704858222204 2216 62-63 -1.0044318120090097 2216 64-65 -0.9725092940971933 2216 66-67 -1.0135262981450168 2216 68-69 -0.8397076086816817 2216 70-71 -0.9005082714032469 2216 72-73 -0.807048396403907 2216 74-75 -0.9014692587725577 2216 76-77 -0.8227345965974457 2216 78-79 -0.8032738073961951 2216 80-81 -0.7407515655451284 2216 82-83 -0.7454752941595792 2216 84-85 -0.8812609287509865 2216 86-87 -0.983244591485942 2216 88-89 -0.8185016477831972 2216 90-91 -0.7993337706029138 2216 92-93 -0.8071017937816976 2216 94-95 -0.892708763067585 2216 96-97 -0.9295548482285945 2216 98-99 -1.0215175452444498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 38.0 10 160.0 11 380.0 12 589.0 13 1021.0 14 2569.0 15 6630.0 16 14633.0 17 27239.0 18 42855.0 19 58149.0 20 71988.0 21 88543.0 22 102245.0 23 88348.0 24 61600.0 25 48355.0 26 51451.0 27 65366.0 28 84310.0 29 110893.0 30 145595.0 31 192257.0 32 253805.0 33 336873.0 34 447652.0 35 624463.0 36 975630.0 37 1377494.0 38 558088.0 39 919.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21233669389898 20.927457868717784 14.2753025408549 22.58490289652833 2 13.894130822684883 22.750930704869806 39.30607261381655 24.04886585862876 3 17.332648875558935 30.235699586962383 31.22937337525466 21.202278162224026 4 11.976430041303763 19.439668117886093 38.16262470150575 30.421277139304394 5 11.122775805281988 39.547006139518 34.605819999366446 14.724398055833571 6 27.04653274079107 39.31273293510128 18.63426017089299 15.006474153214663 7 22.06866061225182 34.82693754573944 24.651181665663838 18.453220176344907 8 26.696558613431925 32.995339500074145 20.964064939472404 19.344036947021532 9 26.93045391491576 13.71681590196242 21.851105578656284 37.50162460446553 10-11 23.722446322403584 27.143448126125612 27.559953124459557 21.574152427011242 12-13 26.36774834053806 25.309350142958753 28.017030738615095 20.30587077788809 14-15 23.89350822829553 24.072061295200232 28.503362285671034 23.531068190833203 16-17 20.17718137400327 26.985509919148328 30.21252192784746 22.624786779000946 18-19 21.006615245577763 25.301516434691873 34.231241821694184 19.46062649803618 20-21 21.928167549235926 24.428250595104988 33.76157890573283 19.882002949926253 22-23 21.426373587235663 24.809645169829658 32.09722839120803 21.666752851726653 24-25 20.875325719596734 25.552400573547306 32.32051904181847 21.251754665037485 26-27 21.481278036542236 24.315188353167883 32.43539630687315 21.76813730341673 28-29 20.764318220811603 24.26360573862366 33.59820114086189 21.373874899702848 30-31 21.87941020652566 24.281088178274995 33.072383806843185 20.76711780835616 32-33 20.612284350425487 25.029014685591765 32.70945623619683 21.649244727785916 34-35 20.282583688832435 25.457908072846124 33.3710407239819 20.88846751433954 36-37 21.81346151277725 24.595301213360077 32.26101710470937 21.330220169153304 38-39 22.23777453508868 24.10890926356978 32.078358999929115 21.57495720141243 40-41 21.131612120655888 24.43473159746349 32.4321344021194 22.001521879761224 42-43 22.51396626043253 24.33939151521212 31.209570788127554 21.937071436227793 44-45 22.546939507952143 24.653333499996492 30.82643122413689 21.97329576791448 46-47 21.906096183211858 24.86134764439108 31.085310196012223 22.147245976384834 48-49 23.079208978679645 25.267313522641388 29.699848257218225 21.953629241460746 50-51 21.778642177781173 26.19603174304204 28.613668219938337 23.41165785923845 52-53 21.031792134417337 26.89560754499101 27.353379144453655 24.719221176138 54-55 21.42849682182603 26.949223529685728 27.046566986510268 24.57571266197797 56-57 22.656942745581645 25.488272930966332 27.20823506517312 24.646549258278906 58-59 21.618620493391774 25.476606235529044 29.02392337451838 23.880849896560804 60-61 22.256601119218594 26.07790113466341 28.096866071498898 23.56863167461909 62-63 21.190086686189012 25.860851766377074 28.23446537124243 24.714596176191485 64-65 20.299449705538184 26.261364013844858 28.644497928647674 24.79468835196928 66-67 22.445611804910357 25.46155524194087 27.90827489586733 24.184558057281443 68-69 22.149526045068548 24.92167245898779 28.278427642732062 24.650373853211597 70-71 21.459496583727404 25.45500216861351 28.21439805264842 24.871103195010665 72-73 22.698276721164163 25.0799723157606 28.325636037181262 23.896114925893976 74-75 22.744097493453076 25.23504549372567 28.211811827991973 23.809045184829284 76-77 21.701811906757126 25.629205160281956 28.766181530393247 23.902801402567675 78-79 22.036193067133507 25.972188261756084 28.21735463151125 23.77426403959916 80-81 21.062256949920233 26.392539652125997 28.373643410056292 24.171559987897474 82-83 21.299510320461167 25.91703186872383 27.736849386984503 25.046608423830502 84-85 21.729884835070905 26.395093547318847 26.77533232902054 25.099689288589705 86-87 21.384636616961288 25.675805022682653 27.591553361850885 25.348004998505175 88-89 21.80070498237544 24.82373728328025 27.961604042090727 25.413953692253582 90-91 21.93242604604015 25.34739012212078 27.687213618898245 25.032970212940832 92-93 21.14586517148295 25.753632010493 27.64660959557952 25.453893222444535 94-95 20.120905308677965 25.91803318688911 28.40825275904596 25.55280874538696 96-97 21.55107664118122 25.557682315489355 27.320622373078823 25.570618670250607 98-99 21.73270154547506 25.729348204651046 27.01635370074652 25.521596549127366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 308.0 1 297.5 2 726.0 3 1189.0 4 832.5 5 690.0 6 1582.5 7 4298.5 8 7847.0 9 7547.0 10 3971.5 11 2001.5 12 1790.5 13 1975.5 14 2421.0 15 3158.5 16 4071.5 17 5137.0 18 6645.0 19 8289.0 20 10219.5 21 12763.5 22 15976.5 23 19990.0 24 24586.0 25 29908.0 26 36010.5 27 42285.5 28 51046.0 29 63739.5 30 74643.0 31 86676.5 32 99554.5 33 108386.0 34 116009.5 35 125344.5 36 129463.0 37 139995.5 38 154267.0 39 144832.5 40 135894.5 41 141308.5 42 145792.5 43 156389.0 44 180630.0 45 216353.0 46 241530.0 47 272224.5 48 302638.0 49 354502.0 50 428186.5 51 362995.5 52 243009.5 53 203529.0 54 171176.0 55 150983.0 56 131794.0 57 98415.5 58 88096.0 59 74247.5 60 50931.0 61 38346.0 62 28460.0 63 21205.0 64 13405.5 65 7301.0 66 5283.0 67 4093.5 68 3248.5 69 2641.5 70 2064.0 71 1767.0 72 1586.0 73 1256.0 74 939.0 75 868.0 76 996.0 77 961.0 78 661.5 79 562.5 80 675.5 81 646.0 82 682.5 83 601.0 84 382.5 85 279.0 86 123.5 87 42.5 88 39.0 89 33.0 90 22.5 91 14.5 92 7.0 93 2.5 94 2.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05703624532674355 2 0.008321709765475041 3 0.0 4 0.0 5 0.00190063741557146 6 0.0 7 0.0 8 0.0 9 0.004503312074732378 10-11 0.0 12-13 0.0 14-15 0.0024913760717625894 16-17 0.004357767768134564 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.938257707940863E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.1129858739832874E-4 54-55 0.0 56-57 7.962129713880441E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0014811273553777594 70-71 1.5410573639768597E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.767029111943434E-4 80-81 7.362829627889439E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 4.4519434959331496E-4 92-93 0.0010444944355843158 94-95 6.59230094590101E-4 96-97 2.654043237960147E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 5840146.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.69512776801029 #Duplication Level Percentage of deduplicated Percentage of total 1 69.66971010099032 16.508326824032025 2 14.816026872526203 7.021352995175646 3 5.6518263234757296 4.017622405720838 4 2.877448413494549 2.727260312144474 5 1.5789947658259171 1.8707241360632294 6 0.9912238589812802 1.4092305591156988 7 0.6750438234701708 1.1196674752292315 8 0.5028390521341358 0.9531868469650829 9 0.3486756867755515 0.7435723452970886 >10 2.2400211454077534 10.337308043937524 >50 0.27535930285964827 4.585559014196116 >100 0.28233815194002126 14.22180128653162 >500 0.05154170178590746 8.546655123837105 >1k 0.035342881209486456 15.120076499157097 >5k 0.002429823083152194 3.7778251695536906 >10k+ 0.0011780960403162154 7.039830963043545 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 68547 1.1737206569835754 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 64879 1.1109140079717186 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32401 0.5547977738912692 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 27840 0.4767004112568419 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 23144 0.39629146257644926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21940 0.37567553961835887 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 20909 0.3580218713710239 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 20701 0.35446031657427746 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 20419 0.3496316701671499 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 19128 0.3275260584238819 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17182 0.29420497364278225 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 15390 0.26352080924004295 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14378 0.2461924753251032 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 13524 0.23156955322692274 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12000 0.2054743151969146 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11117 0.19035483017034163 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9379 0.1605953001859885 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 9316 0.1595165600312047 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 8647 0.1480613669589767 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7998 0.13694863107874358 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 7947 0.1360753652391567 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7832 0.13410623638518626 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 7506 0.12852418415567007 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 7358 0.12599000093490814 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7067 0.12100724879138296 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7066 0.12099012593178321 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6895 0.11806211694022717 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6648 0.1138327706190907 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 6578 0.11263417044710869 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 6449 0.11042532155874185 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6368 0.10903836993116267 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6368 0.10903836993116267 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 6236 0.1067781524639966 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 6136 0.10506586650402233 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 6058 0.10373028345524238 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 6054 0.10366179201684342 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 5945 0.10179540032047145 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 5941 0.10172690888207248 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 5926 0.10147006598807631 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.424571919948577E-5 0.0 5 0.0 0.0 0.0 5.136857879922865E-5 0.0 6 0.0 0.0 0.0 5.136857879922865E-5 0.0 7 0.0 0.0 0.0 5.136857879922865E-5 0.0 8 0.0 0.0 0.0 5.136857879922865E-5 0.0 9 0.0 0.0 0.0 5.136857879922865E-5 0.0 10-11 1.7122859599742884E-5 0.0 0.0 1.027371575984573E-4 0.0 12-13 2.5684289399614326E-5 0.0 0.0 1.7122859599742884E-4 0.0 14-15 3.424571919948577E-5 1.7122859599742884E-5 0.0 2.054743151969146E-4 0.0 16-17 4.280714899935721E-5 2.5684289399614326E-5 0.0 3.1677290259524333E-4 0.0 18-19 5.136857879922865E-5 4.280714899935721E-5 0.0 4.880014985926722E-4 0.0 20-21 5.9930008599100094E-5 5.136857879922865E-5 0.0 0.0010958630143835446 0.0 22-23 6.849143839897154E-5 5.136857879922865E-5 0.0 0.003707099103344334 0.0 24-25 7.705286819884298E-5 5.136857879922865E-5 0.0 0.009177852745462185 0.0 26-27 9.417572779858586E-5 5.136857879922865E-5 0.0 0.015718785112563968 0.0 28-29 1.1986001719820019E-4 5.136857879922865E-5 0.0 0.0359751280190598 0.0 30-31 1.3698287679794307E-4 5.136857879922865E-5 0.0 0.08996350433704911 0.0 32-33 1.5410573639768594E-4 5.136857879922865E-5 0.0 0.17326621628979821 0.0 34-35 1.7122859599742884E-4 6.849143839897154E-5 0.0 0.2777927127164287 0.0 36-37 1.7122859599742884E-4 8.561429799871442E-5 0.0 0.4345439309222749 0.0 38-39 1.7122859599742884E-4 8.561429799871442E-5 0.0 0.6770549914334334 0.0 40-41 1.7122859599742884E-4 1.027371575984573E-4 0.0 0.9995554905647908 0.0 42-43 1.7979002579730026E-4 1.2842144699807162E-4 0.0 1.2674340675729683 0.0 44-45 1.883514555971717E-4 1.712285959974288E-4 0.0 1.5535056829058727 0.0 46-47 1.883514555971717E-4 1.883514555971717E-4 0.0 1.8585066195262927 0.0 48-49 1.883514555971717E-4 1.883514555971717E-4 0.0 2.181811893058838 0.0 50-51 1.883514555971717E-4 2.054743151969146E-4 0.0 2.560269897362155 0.0 52-53 1.883514555971717E-4 2.054743151969146E-4 0.0 2.9506368505170935 0.0 54-55 1.883514555971717E-4 2.054743151969146E-4 0.0 3.2961162272313054 0.0 56-57 1.883514555971717E-4 2.2259717479665748E-4 0.0 3.676825887571989 0.0 58-59 1.883514555971717E-4 2.2259717479665748E-4 0.0 4.1001457840266315 0.0 60-61 1.883514555971717E-4 2.2259717479665748E-4 0.0 4.517626785357763 0.0 62-63 1.883514555971717E-4 2.2259717479665748E-4 0.0 4.944533920898553 0.0 64-65 1.883514555971717E-4 2.5684289399614325E-4 0.0 5.400789637793302 0.0 66-67 1.883514555971717E-4 2.5684289399614325E-4 0.0 5.87444218004139 0.0 68-69 1.883514555971717E-4 2.5684289399614325E-4 0.0 6.39855578952992 0.0 70-71 1.883514555971717E-4 2.654043237960147E-4 0.0 7.03051773020743 0.0 72-73 1.883514555971717E-4 2.7396575359588614E-4 0.0 7.599022353208293 0.0 74-75 1.883514555971717E-4 2.8252718339575754E-4 0.0 8.141474545328148 0.0 76-77 1.883514555971717E-4 2.91088613195629E-4 0.0 8.723626087430006 0.0 78-79 1.883514555971717E-4 2.91088613195629E-4 0.0 9.293046783419456 0.0 80-81 1.883514555971717E-4 2.91088613195629E-4 0.0 9.918493476019265 0.0 82-83 1.883514555971717E-4 2.91088613195629E-4 0.0 10.630804435368567 0.0 84-85 1.883514555971717E-4 2.91088613195629E-4 0.0 11.298767188354537 0.0 86-87 1.883514555971717E-4 3.082114727953719E-4 0.0 11.95458127245449 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 635 0.0 53.460205 2 TCACGCA 760 0.0 44.05251 4 TATTCGC 590 0.0 26.797684 9 AGGGCTA 3720 0.0 25.999926 5 GTACTAG 3550 0.0 25.557938 1 ATCACGC 1335 0.0 25.426895 3 TCAACGA 935 0.0 24.368917 3 CTATTGA 3830 0.0 24.040125 9 TAATAAT 2765 0.0 23.039719 3 AGTACTT 51390 0.0 22.524815 12-13 TAGGCAT 5170 0.0 22.39549 5 GCTATTG 4230 0.0 21.986132 8 GGGCTAT 4930 0.0 21.599146 6 TAACGCA 590 0.0 21.279604 4 CTGTGCG 4900 0.0 21.068192 9 TTCAACG 1000 0.0 20.926374 2 TAGTACT 5170 0.0 20.86648 4 GGCTATT 4885 0.0 20.655857 7 CTAGGCA 5750 0.0 20.459944 4 TTCTGGC 3125 0.0 20.386417 9 >>END_MODULE