##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765310_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7479708 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.248757972904826 33.0 31.0 34.0 31.0 34.0 2 32.27849777023381 34.0 31.0 34.0 31.0 34.0 3 32.30211607191083 34.0 31.0 34.0 30.0 34.0 4 35.766496766986094 37.0 35.0 37.0 35.0 37.0 5 35.79789585368841 37.0 35.0 37.0 35.0 37.0 6 35.892241247920374 37.0 37.0 37.0 35.0 37.0 7 35.852420575776485 37.0 37.0 37.0 35.0 37.0 8 35.879712817666146 37.0 37.0 37.0 35.0 37.0 9 37.57597034536642 39.0 38.0 39.0 35.0 39.0 10-11 37.509375031752576 39.0 38.0 39.0 35.0 39.0 12-13 37.493853837609706 39.0 38.0 39.0 35.0 39.0 14-15 38.89249954677375 41.0 39.0 41.0 36.0 41.0 16-17 38.85135061422184 41.0 39.0 41.0 35.5 41.0 18-19 38.829512729641316 41.0 39.0 41.0 35.0 41.0 20-21 38.810423749162396 41.0 39.0 41.0 35.0 41.0 22-23 38.717085011874794 40.5 39.0 41.0 35.0 41.0 24-25 38.644180762136706 40.5 38.0 41.0 35.0 41.0 26-27 38.52676768932691 40.0 38.0 41.0 34.0 41.0 28-29 38.39785697516534 40.0 38.0 41.0 34.0 41.0 30-31 38.19747475436207 40.0 38.0 41.0 34.0 41.0 32-33 37.97008245509049 40.0 38.0 41.0 33.5 41.0 34-35 37.840778008981104 40.0 38.0 41.0 33.0 41.0 36-37 37.69819851256226 40.0 37.5 41.0 33.0 41.0 38-39 37.52534463912228 40.0 37.0 41.0 33.0 41.0 40-41 37.30276810271204 40.0 37.0 41.0 32.0 41.0 42-43 37.07653146352772 40.0 36.5 41.0 31.5 41.0 44-45 36.774844017975035 40.0 36.0 41.0 31.0 41.0 46-47 36.406285111664786 39.0 36.0 41.0 30.0 41.0 48-49 36.04943495120398 39.0 35.0 41.0 29.5 41.0 50-51 34.806435023934085 38.0 34.0 40.0 27.5 40.5 52-53 34.7589324342608 38.0 34.5 39.5 27.0 40.0 54-55 35.60454438863121 39.0 35.0 40.5 28.5 41.0 56-57 35.63870661795888 39.0 35.0 41.0 29.0 41.0 58-59 35.44003295315807 38.5 35.0 41.0 28.5 41.0 60-61 35.11424169232275 38.0 35.0 40.0 28.0 41.0 62-63 34.703454399556776 37.0 35.0 40.0 27.5 41.0 64-65 34.411343664752685 37.0 34.5 40.0 27.0 41.0 66-67 34.129630194119876 36.0 34.0 39.0 26.0 41.0 68-69 33.90270649602898 36.0 34.0 39.0 26.0 41.0 70-71 33.55453147368854 35.0 34.0 39.0 26.0 41.0 72-73 33.18423847561964 35.0 34.0 37.5 26.0 39.5 74-75 32.802503854428544 35.0 34.0 37.0 25.5 39.0 76-77 32.45125718811483 35.0 34.0 36.5 25.0 39.0 78-79 32.16312802585342 35.0 34.0 36.0 24.0 38.0 80-81 31.869901538937082 35.0 33.0 36.0 23.5 37.0 82-83 31.627257708456 35.0 33.0 35.0 23.0 37.0 84-85 31.393939910488484 35.0 33.0 35.0 20.5 36.0 86-87 31.148435540531796 35.0 33.0 35.0 20.0 36.0 88-89 30.952714597949544 35.0 33.0 35.0 18.5 36.0 90-91 30.73957272128805 35.0 33.0 35.0 16.0 35.5 92-93 30.496984106866204 35.0 33.0 35.0 8.5 35.0 94-95 30.238438519257706 35.0 32.0 35.0 2.0 35.0 96-97 29.99568806696732 35.0 32.0 35.0 2.0 35.0 98-99 29.71029357028376 35.0 32.0 35.0 2.0 35.0 100 29.186541907785706 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.437869501527004 1101 2 0.5209814450719392 1101 3 0.5698583919922342 1101 4 0.43719724033787344 1101 5 0.45088250236398153 1101 6 0.449434640409514 1101 7 0.5221825780962419 1101 8 0.4563636789815533 1101 9 0.5919280131457327 1101 10-11 0.540289362425824 1101 12-13 0.5396071850226107 1101 14-15 0.74018488584273 1101 16-17 0.7267455705517065 1101 18-19 0.6957525759032563 1101 20-21 0.6510060213450615 1101 22-23 0.6605858234234958 1101 24-25 0.6881022136537922 1101 26-27 0.7404579165181318 1101 28-29 0.7377427462412598 1101 30-31 0.7559101685072349 1101 32-33 0.7070721686444728 1101 34-35 0.7725591193869406 1101 36-37 0.8531727932134316 1101 38-39 0.8080123575710374 1101 40-41 0.8052424556363178 1101 42-43 0.8505888004149256 1101 44-45 0.9246461525080747 1101 46-47 0.9579809373517065 1101 48-49 1.0357474580617563 1101 50-51 0.9578854095968907 1101 52-53 0.9446840765681657 1101 54-55 1.1050795196088714 1101 56-57 1.1372186984078425 1101 58-59 1.0861436733963217 1101 60-61 1.0638239674127021 1101 62-63 1.1273411291053392 1101 64-65 1.0693952424841129 1101 66-67 1.04895966053585 1101 68-69 1.0714476855024415 1101 70-71 1.0340761033726054 1101 72-73 0.9175876348481609 1101 74-75 0.8917311479998062 1101 76-77 0.84415641593381 1101 78-79 0.961761274628735 1101 80-81 1.0218775454886249 1101 82-83 0.9560287516522017 1101 84-85 0.9944763126736937 1101 86-87 1.0025002268890972 1101 88-89 1.0777937138020341 1101 90-91 0.9959393385269308 1101 92-93 1.1519328113508323 1101 94-95 1.0866985820644857 1101 96-97 1.0807146257273708 1101 98-99 1.1167978735020085 1101 100 1.1136957891016124 1102 1 0.3904030574665214 1102 2 0.522144919132117 1102 3 0.5273144949432549 1102 4 0.4307202716564973 1102 5 0.4290529268332577 1102 6 0.3769618406714983 1102 7 0.419112702483325 1102 8 0.40644404660992706 1102 9 0.4917000238904876 1102 10-11 0.49037241629724804 1102 12-13 0.563793654329011 1102 14-15 0.7043915837502723 1102 16-17 0.7217049479403741 1102 18-19 0.6705892007179983 1102 20-21 0.6925732507042213 1102 22-23 0.6873704587695713 1102 24-25 0.6311789369369336 1102 26-27 0.5784768252344037 1102 28-29 0.6344145810807689 1102 30-31 0.6988638274534509 1102 32-33 0.7597870949511716 1102 34-35 0.7669496881172506 1102 36-37 0.7601049517349097 1102 38-39 0.7614115189444775 1102 40-41 0.7967069514163185 1102 42-43 0.7722883882473184 1102 44-45 0.8160406974603518 1102 46-47 0.8046056822181598 1102 48-49 0.8448644425969931 1102 50-51 0.8856743531475146 1102 52-53 0.8947169519486664 1102 54-55 1.0295052868567964 1102 56-57 1.0401428367338568 1102 58-59 1.0002649725936763 1102 60-61 0.9311305077608409 1102 62-63 0.934856490842769 1102 64-65 0.9057639907694934 1102 66-67 0.9392484525717819 1102 68-69 0.9054613396626578 1102 70-71 0.8279832886202101 1102 72-73 0.8276495576466516 1102 74-75 0.834656687406671 1102 76-77 0.8420366922775528 1102 78-79 0.8508916078070357 1102 80-81 0.9136098689639489 1102 82-83 0.8982013225752361 1102 84-85 0.8868347689694751 1102 86-87 0.9406436184903555 1102 88-89 0.8651694512419184 1102 90-91 0.908422333814439 1102 92-93 0.9553383403112576 1102 94-95 1.0002428286161837 1102 96-97 1.0425053589775075 1102 98-99 1.0767726799826782 1102 100 1.0762048607958228 1103 1 0.3656849477472832 1103 2 0.4562029236553542 1103 3 0.4852403129034073 1103 4 0.37917263569434567 1103 5 0.3706322497122656 1103 6 0.34120651063222596 1103 7 0.37659088049868217 1103 8 0.3609399817253518 1103 9 0.46897320990493796 1103 10-11 0.486629720951548 1103 12-13 0.5349548561824307 1103 14-15 0.6673574904201658 1103 16-17 0.6660765733370297 1103 18-19 0.6646738810385244 1103 20-21 0.6853497791018626 1103 22-23 0.6874861996416897 1103 24-25 0.6741589353448632 1103 26-27 0.6393334276114402 1103 28-29 0.651009497701196 1103 30-31 0.6771024707458864 1103 32-33 0.7001705275206902 1103 34-35 0.7392245241886499 1103 36-37 0.6999398952083027 1103 38-39 0.6629525689906828 1103 40-41 0.6581340115705316 1103 42-43 0.648885287798457 1103 44-45 0.6098928177132024 1103 46-47 0.6392258436488589 1103 48-49 0.6517842514085146 1103 50-51 0.6225355248522888 1103 52-53 0.6983056543169397 1103 54-55 0.8137478426126492 1103 56-57 0.792572568415892 1103 58-59 0.794885478495658 1103 60-61 0.8063222309547697 1103 62-63 0.8386418848357451 1103 64-65 0.8058697478964021 1103 66-67 0.7283428345954732 1103 68-69 0.7083357329453221 1103 70-71 0.715106315257465 1103 72-73 0.633097825091788 1103 74-75 0.6346113410681795 1103 76-77 0.6711828570215914 1103 78-79 0.69425865030437 1103 80-81 0.717714500982499 1103 82-83 0.7117854910499268 1103 84-85 0.6390421242013353 1103 86-87 0.6803354879365244 1103 88-89 0.7557798918705387 1103 90-91 0.8019861345606003 1103 92-93 0.8287287821805691 1103 94-95 0.7633505533255907 1103 96-97 0.8089650656824148 1103 98-99 0.7869688252897475 1103 100 0.8601915799964992 1104 1 0.31057158046053956 1104 2 0.3711113545490292 1104 3 0.4030162637257746 1104 4 0.3174957244939236 1104 5 0.3551477256203057 1104 6 0.32092185462669676 1104 7 0.37811332868574254 1104 8 0.3634222848532147 1104 9 0.43841799613422694 1104 10-11 0.43035991199192125 1104 12-13 0.41045482367440655 1104 14-15 0.49696519306814224 1104 16-17 0.49856561017590195 1104 18-19 0.5279015964795803 1104 20-21 0.48408401243492705 1104 22-23 0.49318622798235623 1104 24-25 0.5124001232632764 1104 26-27 0.5046873854953731 1104 28-29 0.522051535742925 1104 30-31 0.487819801752174 1104 32-33 0.4883722266507604 1104 34-35 0.5098143907668344 1104 36-37 0.4580778254181652 1104 38-39 0.41671665321894835 1104 40-41 0.47351246366893207 1104 42-43 0.43037609088471385 1104 44-45 0.46228139266556667 1104 46-47 0.43951450568052763 1104 48-49 0.4155084186832525 1104 50-51 0.36199278106331434 1104 52-53 0.4513061076709022 1104 54-55 0.5955507029583984 1104 56-57 0.5462477995734432 1104 58-59 0.554528866558968 1104 60-61 0.5520255008664634 1104 62-63 0.49628213454486314 1104 64-65 0.4950749493210935 1104 66-67 0.5292760303180941 1104 68-69 0.5020701265106382 1104 70-71 0.4843539564694481 1104 72-73 0.497129130561774 1104 74-75 0.5069330391660856 1104 76-77 0.47624653912185266 1104 78-79 0.45398940780642505 1104 80-81 0.5115510893164732 1104 82-83 0.5038737564705009 1104 84-85 0.48545393718247354 1104 86-87 0.5005648451317555 1104 88-89 0.5347224526298824 1104 90-91 0.49905160721783304 1104 92-93 0.5156338375147698 1104 94-95 0.5752484927212222 1104 96-97 0.5806754810294699 1104 98-99 0.5876887516629168 1104 100 0.6361917908260999 1105 1 0.29732427888151136 1105 2 0.36826390039973944 1105 3 0.3952808799055276 1105 4 0.31601690720025033 1105 5 0.3306865651899997 1105 6 0.32334141153034324 1105 7 0.3529832254457119 1105 8 0.3253854878964546 1105 9 0.4350371528305317 1105 10-11 0.4301538924994972 1105 12-13 0.45111616279282885 1105 14-15 0.5617479810910382 1105 16-17 0.5189161025611071 1105 18-19 0.5557960014231611 1105 20-21 0.5398022185870701 1105 22-23 0.48909479669709555 1105 24-25 0.5050647123598822 1105 26-27 0.45566577813239206 1105 28-29 0.5078830071095908 1105 30-31 0.5127387143277957 1105 32-33 0.4761643995924061 1105 34-35 0.5092276351173979 1105 36-37 0.48555302068506734 1105 38-39 0.48460083441075597 1105 40-41 0.5463854468601497 1105 42-43 0.5391887884148545 1105 44-45 0.4944262251961291 1105 46-47 0.41649637803366346 1105 48-49 0.50558706324599 1105 50-51 0.46282187851728906 1105 52-53 0.47303006333893194 1105 54-55 0.5325623805679527 1105 56-57 0.48332904668362175 1105 58-59 0.5101419393369326 1105 60-61 0.4915229953302074 1105 62-63 0.5042933142050288 1105 64-65 0.4781743870974893 1105 66-67 0.44045073994988826 1105 68-69 0.4221933769135475 1105 70-71 0.36839152758513194 1105 72-73 0.40011994338864554 1105 74-75 0.49270031905101064 1105 76-77 0.41101179066627935 1105 78-79 0.43824332696872403 1105 80-81 0.4084440561226508 1105 82-83 0.39274237436515946 1105 84-85 0.46233342033003666 1105 86-87 0.43505009494969826 1105 88-89 0.46262018091334056 1105 90-91 0.5208869154562272 1105 92-93 0.4553236995768586 1105 94-95 0.4841745009722054 1105 96-97 0.45240207744266314 1105 98-99 0.4621017374548799 1105 100 0.5400533600685762 1106 1 0.152038196777454 1106 2 0.2829530508218596 1106 3 0.30642403419940933 1106 4 0.284586339488726 1106 5 0.32517249804028836 1106 6 0.2712278492440703 1106 7 0.27735097522878505 1106 8 0.2746039541187173 1106 9 0.27506816212571294 1106 10-11 0.3521515924662353 1106 12-13 0.34361450174426267 1106 14-15 0.3990716622404733 1106 16-17 0.36242288061999517 1106 18-19 0.38445686860371353 1106 20-21 0.3694177636369389 1106 22-23 0.40604159832275144 1106 24-25 0.42537751468378104 1106 26-27 0.420856762742595 1106 28-29 0.41897780332786994 1106 30-31 0.36573749703514835 1106 32-33 0.3848895309524991 1106 34-35 0.38140137552747433 1106 36-37 0.415815460401312 1106 38-39 0.46035199086626477 1106 40-41 0.45783262643221434 1106 42-43 0.37385147388104656 1106 44-45 0.37598037822084507 1106 46-47 0.374268935045734 1106 48-49 0.4205488740231047 1106 50-51 0.38920022803123544 1106 52-53 0.40504693147160964 1106 54-55 0.45437479651302937 1106 56-57 0.4479723641722657 1106 58-59 0.496387577279954 1106 60-61 0.47760343123481164 1106 62-63 0.461911750473881 1106 64-65 0.4089346601867945 1106 66-67 0.3963460943941399 1106 68-69 0.31242744704760383 1106 70-71 0.3525235803276914 1106 72-73 0.3687728016066387 1106 74-75 0.37498560841626016 1106 76-77 0.34659387533745445 1106 78-79 0.29351635990821734 1106 80-81 0.38185561111800226 1106 82-83 0.3353192310297466 1106 84-85 0.3842617270082265 1106 86-87 0.39720596871898906 1106 88-89 0.41063189500639297 1106 90-91 0.38931184178944633 1106 92-93 0.4222861699156013 1106 94-95 0.4229298781271602 1106 96-97 0.479937446159699 1106 98-99 0.5064151769761693 1106 100 0.5917495727311852 1107 1 0.14714892315002714 1107 2 0.283811021301311 1107 3 0.24157014732350746 1107 4 0.2476598204831575 1107 5 0.31763597219040207 1107 6 0.2406514433703677 1107 7 0.25266822981518544 1107 8 0.22131584524478143 1107 9 0.28386923302846157 1107 10-11 0.3134363751906264 1107 12-13 0.34838784990134997 1107 14-15 0.3441064575625816 1107 16-17 0.3479616445307485 1107 18-19 0.3628161302381159 1107 20-21 0.33454644860025695 1107 22-23 0.31710993909712926 1107 24-25 0.3959109451211589 1107 26-27 0.3382289012718118 1107 28-29 0.34511044370749033 1107 30-31 0.39800381743712165 1107 32-33 0.40170742320413666 1107 34-35 0.3971492313969236 1107 36-37 0.4340522528276196 1107 38-39 0.4533303710710399 1107 40-41 0.44702433779202266 1107 42-43 0.45026650633717935 1107 44-45 0.4869826627827294 1107 46-47 0.5052117089009798 1107 48-49 0.5577154391124353 1107 50-51 0.4403377351058495 1107 52-53 0.5481226102142216 1107 54-55 0.5964519496741545 1107 56-57 0.5998359061113305 1107 58-59 0.5682204462218436 1107 60-61 0.5672281679426376 1107 62-63 0.5975399448778731 1107 64-65 0.5761519927287253 1107 66-67 0.5657794484319254 1107 68-69 0.5263062091442805 1107 70-71 0.4665322358839816 1107 72-73 0.4458259766808865 1107 74-75 0.4651465391016174 1107 76-77 0.4860057858595823 1107 78-79 0.543775978857937 1107 80-81 0.5249934827748071 1107 82-83 0.5464491989263571 1107 84-85 0.5826391505648694 1107 86-87 0.5668240449944655 1107 88-89 0.5942089255497898 1107 90-91 0.5683081763415316 1107 92-93 0.6178607749795475 1107 94-95 0.5337170026114713 1107 96-97 0.5714546509210514 1107 98-99 0.592788086220132 1107 100 0.5832269775636512 1108 1 0.18028131103530143 1108 2 0.16794986282578606 1108 3 0.20444683521888152 1108 4 0.18866343802744723 1108 5 0.2925809155287382 1108 6 0.2189870641822651 1108 7 0.3001761083566521 1108 8 0.260670851069996 1108 9 0.3320122901975182 1108 10-11 0.3392579598946597 1108 12-13 0.34828430058673376 1108 14-15 0.3754020308588011 1108 16-17 0.3909536339474542 1108 18-19 0.40393576732876113 1108 20-21 0.3502365585640206 1108 22-23 0.35883724898771874 1108 24-25 0.3829698946277347 1108 26-27 0.3735780788389107 1108 28-29 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-0.961999856091218 2316 48-49 -0.8703064867380093 2316 50-51 -0.8007577817629468 2316 52-53 -0.8599967600286291 2316 54-55 -1.0182921330646053 2316 56-57 -0.9785971491927725 2316 58-59 -1.1053776604936587 2316 60-61 -0.9818103247937344 2316 62-63 -0.891413119917992 2316 64-65 -0.8733015379047444 2316 66-67 -0.8145312245413976 2316 68-69 -0.852058252977308 2316 70-71 -0.8314478949115127 2316 72-73 -0.7757448031751437 2316 74-75 -0.6824471055509207 2316 76-77 -0.7299300603174217 2316 78-79 -0.6867215421399564 2316 80-81 -0.6541930586381248 2316 82-83 -0.6226829938561202 2316 84-85 -0.5965864680979003 2316 86-87 -0.6820919654584365 2316 88-89 -0.6843918709507548 2316 90-91 -0.6574605879014754 2316 92-93 -0.5790534744321967 2316 94-95 -0.5111498446233682 2316 96-97 -0.5667834302447297 2316 98-99 -0.7021572747463196 2316 100 -0.8379357378055943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20702.0 3 3573.0 4 3438.0 5 3972.0 6 4499.0 7 5069.0 8 6116.0 9 7345.0 10 9504.0 11 13212.0 12 19336.0 13 26660.0 14 27411.0 15 33079.0 16 43127.0 17 58523.0 18 58866.0 19 35615.0 20 30575.0 21 29846.0 22 31592.0 23 36995.0 24 42188.0 25 47094.0 26 53720.0 27 63145.0 28 75285.0 29 90844.0 30 111437.0 31 139996.0 32 181066.0 33 244573.0 34 355928.0 35 572186.0 36 1050915.0 37 1873713.0 38 1691702.0 39 376354.0 40 507.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.341550593747414 18.233422415300076 13.28008403669538 24.14494295425713 2 15.05453536385267 21.669454131092056 37.46297171975999 25.813038785295284 3 18.748192512226503 26.857453277741104 29.454116214133236 24.94023799589916 4 13.280247520274873 17.959739843909567 35.56983421799251 33.19017841782305 5 13.846551185207623 36.734443198685426 33.62039429546964 15.798611320637319 6 31.163604603309796 34.716641439909225 18.349647945494137 15.770106011286844 7 26.101027077197624 32.00322631956952 22.49171595319694 19.40403065003591 8 27.273838329698524 31.59898232535925 21.381260672170725 19.745918672771502 9 27.557919435205374 14.845364857643176 19.936493025472856 37.66022268167859 10-11 25.025584875135493 25.49491444088189 26.663157552056294 22.81634313192632 12-13 26.86410370418705 23.412527394300188 26.7524396695661 22.97092923194666 14-15 25.200599221911276 23.22260724460817 25.207963302243748 26.368830231236807 16-17 23.421779316248173 26.240277963526793 26.915038869403652 23.422903850821385 18-19 24.278813387524426 25.500047801492855 28.40834686369987 21.812791947282854 20-21 24.384399869207567 25.003601880047487 27.702019275548107 22.909978975196836 22-23 23.44350104884968 25.59523307361499 26.147986563475662 24.813279314059674 24-25 23.4359719945862 26.264798424822704 25.812592525729084 24.486637054862015 26-27 24.10083795039122 25.152338637886178 26.135588669440306 24.611234742282296 28-29 23.52041492118777 24.65310176382487 27.97920251206997 23.847280802917393 30-31 24.756098394292376 24.72778081996494 27.209343580271245 23.306777205471434 32-33 22.643860879607765 25.549240711451592 27.312026763506186 24.494871645434454 34-35 22.728685952066666 25.901455022780727 27.72902028849854 23.640838736654068 36-37 23.987845609940827 25.788088349823767 26.616347875423855 23.607718164811548 38-39 24.402473654167444 24.811593745241506 26.468198608465833 24.31773399212522 40-41 23.632367713907524 25.64194083717495 26.52460300708066 24.20108844183687 42-43 25.130828129163273 25.043287157466516 26.175539917940544 23.650344795429675 44-45 24.8560199299785 24.870151629387617 26.599090111263045 23.674738329370836 46-47 23.93838131836029 25.048553594251278 27.663612826556893 23.34945226083154 48-49 24.347083901253708 25.78948831728194 26.95837312544405 22.9050546560203 50-51 23.30690282359595 26.331160110468538 25.974570218777444 24.387366847158074 52-53 23.04535820421685 26.458720468636486 24.99079842624313 25.505122900903533 54-55 23.76919068854047 25.96549572224812 24.87416901383957 25.391144575371843 56-57 24.368583389900103 24.902827464404076 25.224299760021317 25.504289385674504 58-59 24.05669086426425 24.808068462357408 26.746680216625347 24.38856045675299 60-61 24.03258445962559 25.30450585367782 26.13567612024468 24.527233566451905 62-63 22.583851240877124 25.60951211998665 26.584862656031326 25.221773983104896 64-65 22.659046056901897 25.56303397556055 26.565427171080152 25.212492796457397 66-67 24.10430976775234 25.293002860438087 25.715959328087457 24.88672804372212 68-69 24.320590902923282 24.579668937853654 25.72297906152759 25.37676109769548 70-71 23.38600165919449 25.08386160617924 25.997128034062012 25.533008700564253 72-73 24.62717167565832 24.54320107601264 25.943956269145353 24.885670979183686 74-75 24.815433891848873 24.23044962875136 26.15298855810351 24.801127921296263 76-77 23.7344308299204 25.076991082282106 26.890177785070605 24.298400302726886 78-79 23.919476579556907 24.950723631694956 26.73280053730841 24.396999251439727 80-81 23.203528020004352 25.129140361597695 26.432945332938758 25.23438628545919 82-83 23.32899688742425 25.547505300856667 25.33049112726487 25.793006684454216 84-85 23.67224234639854 25.378362449021242 25.15393639087949 25.795458813700726 86-87 23.817809090126868 24.37465494272677 25.763073339129903 26.044462628016458 88-89 24.00950537400579 23.83794640214912 26.51514745742694 25.63740076641815 90-91 24.39221909725231 24.409429572525276 25.958352257043337 25.239999073179074 92-93 23.440524192401675 24.679715179855396 26.33898463182895 25.54077599591397 94-95 23.073210851103845 24.845108603062442 26.661972012366093 25.41970853346762 96-97 24.295703771711523 24.5076300085566 25.72537415585164 25.47129206388024 98-99 24.475392574882186 24.012785490095247 25.58022656158996 25.931595373432614 100 24.46597686399643 24.335627322434146 25.874350449835422 25.324045363733998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1334.0 1 1883.0 2 1669.5 3 864.5 4 720.5 5 697.5 6 944.5 7 1597.5 8 2433.5 9 2681.5 10 2340.0 11 2207.5 12 2442.5 13 2721.0 14 2981.0 15 3360.5 16 3807.5 17 4244.0 18 4813.0 19 5445.5 20 6324.0 21 7429.5 22 8836.0 23 10617.5 24 12887.0 25 16119.5 26 20749.0 27 26113.0 28 31260.0 29 38717.5 30 47992.5 31 56920.0 32 66674.0 33 77067.5 34 88049.5 35 96599.5 36 104047.0 37 114833.0 38 123791.5 39 132730.0 40 140310.5 41 146321.5 42 155350.5 43 171959.0 44 194560.0 45 215784.0 46 243077.0 47 275426.0 48 368388.5 49 468150.0 50 553270.0 51 515930.0 52 374696.5 53 324973.5 54 298638.0 55 267085.0 56 243459.5 57 218836.0 58 201596.5 59 184157.5 60 157515.5 61 130548.5 62 106297.0 63 82866.5 64 61381.0 65 48714.5 66 38272.5 67 28034.0 68 21492.5 69 17834.5 70 15485.0 71 13208.5 72 11064.0 73 11000.0 74 9272.5 75 7290.5 76 5679.5 77 3942.5 78 2881.5 79 2067.5 80 1552.0 81 1273.0 82 1017.0 83 768.0 84 536.0 85 354.5 86 249.5 87 171.5 88 126.0 89 98.5 90 85.0 91 78.0 92 69.5 93 54.5 94 42.5 95 31.5 96 27.5 97 26.0 98 18.5 99 14.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11612752797301713 2 0.36493403218414405 3 0.3752419212086889 4 0.3726616065760856 5 0.29957051799348317 6 0.13537961642352883 7 0.14888281735062384 8 0.1249246628344315 9 0.2049678944686076 10-11 0.20504811150381808 12-13 0.2172344161028746 14-15 0.1473653784345592 16-17 0.13303995289655693 18-19 0.15136954544214826 20-21 0.1522251938177266 22-23 0.1604741789385361 24-25 0.17201206250297474 26-27 0.16552116740386122 28-29 0.17429824800647298 30-31 0.21585067224549406 32-33 0.21141199629718166 34-35 0.12951708810023066 36-37 0.08941525524793213 38-39 0.08329202156019995 40-41 0.0826302310197136 42-43 0.0734453804881153 44-45 0.09553848893566433 46-47 0.14227828145162885 48-49 0.2198013612296095 50-51 0.17577557840493238 52-53 0.109095167886233 54-55 0.05886593433861322 56-57 0.073565706040931 58-59 0.10827294327532572 60-61 0.08462228739410682 62-63 0.09372023613756045 64-65 0.10281150012807987 66-67 0.09928195057881939 68-69 0.08917460414230075 70-71 0.1232066813303407 72-73 0.1473653784345592 74-75 0.1910970321301313 76-77 0.19028817702509243 78-79 0.1520313359826346 80-81 0.09003693727081324 82-83 0.12953714235903327 84-85 0.15735239931826214 86-87 0.16913093398833215 88-89 0.15671734778951263 90-91 0.1781486656965753 92-93 0.09911483175546426 94-95 0.33597568247316606 96-97 0.33727920929533617 98-99 0.37616441711360926 100 0.3770869130185296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7479708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.358432984532385 #Duplication Level Percentage of deduplicated Percentage of total 1 79.31796777076502 32.80466854516486 2 11.712946309433708 9.6885820998028 3 3.529126730941619 4.3787745418671244 4 1.5245575402039957 2.5221324343036198 5 0.8700821767314084 1.7992617698691014 6 0.5648799003795741 1.4017528502494767 7 0.3713741618840724 1.0751617370528506 8 0.27221520029511304 0.9006715295021195 9 0.22746085339988928 0.8466682015749479 >10 1.3337547570048154 10.45727996768172 >50 0.13715739935290383 3.9601718498766663 >100 0.11330547423072893 9.793296045156648 >500 0.015330445494279392 4.337425170399986 >1k 0.008412183134414478 6.2451631185307495 >5k 7.145483742469837E-4 2.129936555712432 >10k+ 7.145483742469837E-4 7.659053583254857 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 80206 1.0723145876817652 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 70239 0.9390607226913137 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 37090 0.4958749726593605 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 36490 0.48785326913831395 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 36224 0.4842969805773167 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 32427 0.43353296679496045 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 28400 0.3796939666628697 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 25851 0.3456150962042903 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 25089 0.3354275327325612 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 23362 0.3123383960978156 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 22751 0.3041696280122165 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19817 0.26494349779429893 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 18772 0.2509723641618095 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 16292 0.2178159896081505 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 14388 0.1923604504346961 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 14330 0.1915850190943283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12892 0.17235966965555338 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 12119 0.16202504161927175 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 11653 0.15579485188459227 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 10904 0.1457810919891525 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 10745 0.14365534055607518 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10126 0.13537961642352883 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 9957 0.1331201699317674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9794 0.13094094047521643 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 9512 0.12717073982032454 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 9216 0.1232133660832749 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 9134 0.12211706660206521 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8593 0.11488416392725492 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 8349 0.11162200449536265 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7914 0.1058062694426039 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.010851760523272E-5 0.0 0.0 0.0 0.0 2 1.6043407042093088E-4 0.0 0.0 0.0 0.0 3 1.6043407042093088E-4 0.0 0.0 0.0 1.3369505868410905E-5 4 2.005425880261636E-4 0.0 0.0 0.0 1.3369505868410905E-5 5 9.358654107887634E-4 0.0 0.0 0.0 1.3369505868410905E-5 6 0.001858361315709116 0.0 0.0 0.0 2.673901173682181E-5 7 0.0020856429154721013 0.0 0.0 0.0 2.673901173682181E-5 8 0.002312924515235087 0.0 0.0 0.0 2.673901173682181E-5 9 0.0024466195739191957 0.0 0.0 4.010851760523272E-5 2.673901173682181E-5 10-11 0.004231448607352052 0.0 0.0 8.690178814467088E-5 2.673901173682181E-5 12-13 0.008228930862006913 0.0 0.0 1.8048832922354724E-4 2.673901173682181E-5 14-15 0.012988474951161195 6.684752934205453E-6 0.0 2.2059684682877994E-4 2.673901173682181E-5 16-17 0.014927053302080776 1.3369505868410905E-5 0.0 3.275528937760672E-4 2.673901173682181E-5 18-19 0.016003298524487855 1.3369505868410905E-5 0.0 4.8798696419699805E-4 2.673901173682181E-5 20-21 0.020167899602497853 1.3369505868410905E-5 0.0 7.353228227625999E-4 2.673901173682181E-5 22-23 0.0218190335772466 1.3369505868410905E-5 0.0 0.0017380357628934177 2.673901173682181E-5 24-25 0.03195311902550206 1.3369505868410905E-5 0.0 0.003703353125549821 2.673901173682181E-5 26-27 0.06255591795829463 1.3369505868410905E-5 0.0 0.008028388273980749 4.010851760523272E-5 28-29 0.0695214305157367 1.3369505868410905E-5 0.0 0.02412527333954748 4.010851760523272E-5 30-31 0.07073805554976209 1.3369505868410905E-5 0.0 0.06976208162136811 4.010851760523272E-5 32-33 0.07161375818414302 1.3369505868410905E-5 0.0 0.1427194751452864 4.010851760523272E-5 34-35 0.07228891823049777 2.673901173682181E-5 0.0 0.2417407203596718 4.010851760523272E-5 36-37 0.07380635714656242 3.3423764671027265E-5 0.0 0.3888186544180602 4.010851760523272E-5 38-39 0.07528368754502182 4.6793270539438166E-5 0.0 0.6106120720220629 5.347802347364362E-5 40-41 0.0766206381318629 5.347802347364362E-5 0.0 0.9100488949568619 5.347802347364362E-5 42-43 0.08010339441058395 5.347802347364362E-5 0.0 1.1396233649762797 5.347802347364362E-5 44-45 0.08400729012415993 5.347802347364362E-5 0.0 1.3845259734738309 5.347802347364362E-5 46-47 0.08575201063998755 5.347802347364362E-5 0.0 1.663701310265053 6.0162776407849075E-5 48-49 0.08677477783892099 5.347802347364362E-5 0.0 1.9241860778522368 8.021703521046544E-5 50-51 0.08848607459007758 5.347802347364362E-5 0.0 2.2102734491774276 8.021703521046544E-5 52-53 0.08926819068337963 5.347802347364362E-5 0.0 2.5382407976354155 1.0027129401308179E-4 54-55 0.09160116945741732 5.347802347364362E-5 0.0 2.838299302593096 1.0695604694728724E-4 56-57 0.09622033373495328 5.347802347364362E-5 0.0 3.1561593046145653 1.136407998814927E-4 58-59 0.09766424036874166 5.347802347364362E-5 0.0 3.486880771281446 1.2032555281569815E-4 60-61 0.09852657349725416 5.347802347364362E-5 0.0 3.793222943997279 1.3369505868410906E-4 62-63 0.09902792996731957 6.684752934205453E-5 0.0 4.1097192564201706 1.4706456455251997E-4 64-65 0.09946912366097714 6.684752934205453E-5 0.0 4.456149892482434 1.4706456455251997E-4 66-67 0.1002712940130818 9.358654107887635E-5 0.0 4.819446160197698 1.5374931748672542E-4 68-69 0.10128737645908102 9.358654107887635E-5 0.0 5.247183446198703 1.7380357628934178E-4 70-71 0.10245720822256697 9.358654107887635E-5 0.0 5.745291126338087 1.8048832922354724E-4 72-73 0.10538513000774896 9.358654107887635E-5 0.0 6.166156486322729 2.673901173682181E-4 74-75 0.10823283475772048 9.358654107887635E-5 0.0 6.576352980624377 2.673901173682181E-4 76-77 0.10904837461569356 1.0695604694728724E-4 0.0 7.030488355962559 2.673901173682181E-4 78-79 0.1097101651561799 1.136407998814927E-4 0.0 7.4806797270695595 2.673901173682181E-4 80-81 0.1108398884020606 1.2032555281569815E-4 0.0 7.985639278966505 2.9412912910503993E-4 82-83 0.11136798388386285 1.2032555281569815E-4 0.0 8.554090614232535 3.409223996444781E-4 84-85 0.11348036581107177 1.2032555281569815E-4 0.0 9.086531185441999 3.4760715257868357E-4 86-87 0.1174377395481214 1.2032555281569815E-4 0.0 9.623203205258815 3.4760715257868357E-4 88 0.11849393051172585 1.2032555281569815E-4 0.0 10.053320263304396 3.609766584470945E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 5705 0.0 24.685154 1 TCTACAC 7260 0.0 22.832653 3 TACACTC 6885 0.0 22.003626 5 ATCTACA 6740 0.0 21.867092 2 CTACACT 9220 0.0 20.271984 4 AGTACTT 23960 0.0 19.52935 12-13 GAGTACT 22790 0.0 18.758223 12-13 CGTTAAC 660 0.0 17.840885 1 GTATTAG 2855 0.0 17.487192 1 GTACTAG 2270 0.0 17.221561 1 CATGGGG 14065 0.0 17.205212 4 CACTCTT 8830 0.0 17.096918 7 TACTTTT 27735 0.0 17.065838 14-15 CATGGGT 6625 0.0 16.84221 4 TCTAACG 1210 0.0 16.733612 2 GTACTTT 25950 0.0 16.610376 14-15 TATACCG 1105 0.0 16.60522 5 TAGGCAT 3550 0.0 16.301184 5 CTAACGC 1195 0.0 16.157207 3 AGTACTC 2540 0.0 15.929682 5 >>END_MODULE