##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765310_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7479708 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48304706547368 34.0 31.0 34.0 31.0 34.0 2 32.62392542596583 34.0 31.0 34.0 31.0 34.0 3 32.67207610243609 34.0 31.0 34.0 31.0 34.0 4 36.187157573530946 37.0 35.0 37.0 35.0 37.0 5 36.12171370861002 37.0 35.0 37.0 35.0 37.0 6 36.17353631986703 37.0 37.0 37.0 35.0 37.0 7 36.12430712535837 37.0 36.0 37.0 35.0 37.0 8 36.12799189487076 37.0 36.0 37.0 35.0 37.0 9 37.861961857334535 39.0 38.0 39.0 35.0 39.0 10-11 37.80733686395244 39.0 38.0 39.0 35.0 39.0 12-13 37.77493158823847 39.0 38.0 39.0 35.0 39.0 14-15 39.15096077280022 40.0 39.0 41.0 36.0 41.0 16-17 39.12676744867581 40.0 38.5 41.0 36.0 41.0 18-19 39.14646634601243 40.0 39.0 41.0 36.0 41.0 20-21 39.12311142360103 40.0 39.0 41.0 36.0 41.0 22-23 39.054095427254644 40.0 39.0 41.0 35.5 41.0 24-25 38.993158489609485 40.0 38.0 41.0 35.0 41.0 26-27 38.864954754918244 40.0 38.0 41.0 35.0 41.0 28-29 38.7353964218924 40.0 38.0 41.0 35.0 41.0 30-31 38.575666924430735 40.0 38.0 41.0 34.5 41.0 32-33 38.35228266932346 40.0 38.0 41.0 34.0 41.0 34-35 38.26147698546521 40.0 38.0 41.0 34.0 41.0 36-37 38.145562767958324 40.0 38.0 41.0 34.0 41.0 38-39 37.98546841668151 40.0 37.5 41.0 33.0 41.0 40-41 37.7874415685746 40.0 37.0 41.0 33.0 41.0 42-43 37.56229995876845 40.0 37.0 41.0 33.0 41.0 44-45 37.277539377205635 40.0 36.5 41.0 32.5 41.0 46-47 36.974765926691255 40.0 36.0 41.0 31.5 41.0 48-49 36.69999483937073 39.0 35.5 41.0 31.0 41.0 50-51 36.43397937459591 39.0 35.0 41.0 31.0 41.0 52-53 36.30195503621265 39.0 35.0 41.0 30.5 41.0 54-55 36.294287357207 39.0 35.0 41.0 31.0 41.0 56-57 36.127489736230345 39.0 35.0 41.0 31.0 41.0 58-59 35.99721326019679 39.0 35.0 41.0 31.0 41.0 60-61 35.690366054396776 38.0 35.0 40.0 30.0 41.0 62-63 35.369657411759924 37.0 35.0 40.0 30.0 41.0 64-65 35.13984864115016 37.0 35.0 40.0 30.0 41.0 66-67 34.803938335560694 36.0 35.0 39.0 29.5 41.0 68-69 34.465775067689805 36.0 34.5 39.0 29.0 41.0 70-71 34.10164547867377 35.0 34.0 38.5 29.0 40.5 72-73 33.733503166166386 35.0 34.0 37.0 29.0 39.5 74-75 33.3881687627378 35.0 34.0 37.0 28.5 39.0 76-77 31.943205804290756 34.5 32.0 35.5 25.5 37.5 78-79 32.74531432243077 35.0 33.5 36.0 27.5 37.0 80-81 32.70612983822363 35.0 34.0 35.5 29.0 37.0 82-83 32.53803403822716 35.0 34.0 35.0 28.0 37.0 84-85 32.35349936120501 35.0 34.0 35.0 27.5 36.0 86-87 32.182491482287816 35.0 34.0 35.0 27.0 36.0 88-89 32.033652316373846 35.0 33.5 35.0 27.0 36.0 90-91 31.90360673170664 35.0 33.0 35.0 27.0 35.5 92-93 31.758652811045565 35.0 33.0 35.0 26.0 35.0 94-95 31.65807087656363 35.0 33.0 35.0 25.5 35.0 96-97 31.543679378927628 35.0 33.0 35.0 25.0 35.0 98-99 31.406858262381363 35.0 33.0 35.0 25.0 35.0 100 31.0606816469306 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4090497432333038 1101 2 0.3999834182943687 1101 3 0.40077818502919627 1101 4 0.25674398352731487 1101 5 0.3246112125397005 1101 6 0.30607816664495147 1101 7 0.29860927865231446 1101 8 0.3235796770810282 1101 9 0.3525201671777012 1101 10-11 0.4136238316344887 1101 12-13 0.43775963766186266 1101 14-15 0.5348435147338151 1101 16-17 0.5292385083095539 1101 18-19 0.5195960091204128 1101 20-21 0.47721173651171256 1101 22-23 0.5200769411004771 1101 24-25 0.5462296802259559 1101 26-27 0.5535891607810512 1101 28-29 0.5557552695366113 1101 30-31 0.5827528274999452 1101 32-33 0.6223936319454069 1101 34-35 0.5377023299688588 1101 36-37 0.621149291730049 1101 38-39 0.7397146062777935 1101 40-41 0.7533391652451868 1101 42-43 0.7835855374089178 1101 44-45 0.7648073329744207 1101 46-47 0.7920637492898237 1101 48-49 0.7699832191696956 1101 50-51 0.8020670142959077 1101 52-53 0.7993730053519457 1101 54-55 0.887724255935936 1101 56-57 0.8923176709023934 1101 58-59 0.8482420497596266 1101 60-61 0.8497132816448669 1101 62-63 0.819768476600558 1101 64-65 0.7530909995063766 1101 66-67 0.8459832563003076 1101 68-69 0.8424473218501305 1101 70-71 0.8595339216799331 1101 72-73 0.889737600905832 1101 74-75 0.8681222938231841 1101 76-77 0.9859763322778043 1101 78-79 0.9497742860955398 1101 80-81 0.9337198838684913 1101 82-83 0.8789044726825139 1101 84-85 0.9087908066657704 1101 86-87 0.9374111148070057 1101 88-89 0.9690217909273144 1101 90-91 0.9734526144050122 1101 92-93 1.0302773255884823 1101 94-95 1.0772496014064892 1101 96-97 1.1275564433380687 1101 98-99 1.1331693752319012 1101 100 1.2338112111246637 1102 1 0.376086444512012 1102 2 0.3321982053134036 1102 3 0.3809455864031861 1102 4 0.2584351786365886 1102 5 0.29237251134112796 1102 6 0.27798022310030035 1102 7 0.3142085434844901 1102 8 0.3233505656907596 1102 9 0.29319460844047285 1102 10-11 0.3270182837459714 1102 12-13 0.3566771378181315 1102 14-15 0.46954887251043687 1102 16-17 0.5076663489665876 1102 18-19 0.4829371545249259 1102 20-21 0.4748221711394862 1102 22-23 0.49439636488202154 1102 24-25 0.4643983537411387 1102 26-27 0.5544602445628826 1102 28-29 0.5894359804112739 1102 30-31 0.5903825120365553 1102 32-33 0.616351371493316 1102 34-35 0.6190054722592393 1102 36-37 0.6521467369144105 1102 38-39 0.6260940677768403 1102 40-41 0.6678357709719833 1102 42-43 0.6643366051442143 1102 44-45 0.7380069771517554 1102 46-47 0.7388733711954245 1102 48-49 0.737332398002394 1102 50-51 0.7374099247341306 1102 52-53 0.7523462450251159 1102 54-55 0.8210314440524016 1102 56-57 0.8843072014884328 1102 58-59 0.9352449763618935 1102 60-61 0.9333326138506663 1102 62-63 0.8691961956753218 1102 64-65 0.9174152357651266 1102 66-67 0.9609028778210984 1102 68-69 0.9617148063944398 1102 70-71 0.9556007351874953 1102 72-73 0.9626659901122281 1102 74-75 0.9770184850587 1102 76-77 0.9861291570119057 1102 78-79 0.976150427239368 1102 80-81 0.9408677913751333 1102 82-83 0.9443341283929669 1102 84-85 0.9660550906904248 1102 86-87 0.9492732068467475 1102 88-89 1.0289085495063688 1102 90-91 1.0152721785857608 1102 92-93 1.0432670971689042 1102 94-95 1.0492999570042052 1102 96-97 1.0110806931259653 1102 98-99 1.0389481694946383 1102 100 1.0902259092515543 1103 1 0.33382754097794276 1103 2 0.2672093293177866 1103 3 0.2980665842131316 1103 4 0.23502374030461937 1103 5 0.2772311229037072 1103 6 0.26242453144514144 1103 7 0.2798260894503102 1103 8 0.2898251443759676 1103 9 0.3015930207732964 1103 10-11 0.32188782067439803 1103 12-13 0.3303593216989853 1103 14-15 0.4338791914567892 1103 16-17 0.39921401799403355 1103 18-19 0.40619750172928093 1103 20-21 0.4623521871485181 1103 22-23 0.4227446097588654 1103 24-25 0.470106435447164 1103 26-27 0.5225629929453106 1103 28-29 0.4811754528283245 1103 30-31 0.520900185757796 1103 32-33 0.5437707036463948 1103 34-35 0.5277058003508657 1103 36-37 0.5807793433327291 1103 38-39 0.6292350611086519 1103 40-41 0.5954671651770767 1103 42-43 0.5801690557904351 1103 44-45 0.5010999828695333 1103 46-47 0.5293227394187952 1103 48-49 0.5475221547982656 1103 50-51 0.6269600674404998 1103 52-53 0.5844410938616917 1103 54-55 0.6552253346024202 1103 56-57 0.6026362497404207 1103 58-59 0.5805628910353633 1103 60-61 0.6154821339325736 1103 62-63 0.6104251572024708 1103 64-65 0.628582328669772 1103 66-67 0.632783267124978 1103 68-69 0.583910653422933 1103 70-71 0.49839496338847056 1103 72-73 0.5104795795242651 1103 74-75 0.5674963733332348 1103 76-77 0.5661433474523143 1103 78-79 0.6183993519633688 1103 80-81 0.6346624767161586 1103 82-83 0.6056583357376368 1103 84-85 0.7226729985484965 1103 86-87 0.6737645774708554 1103 88-89 0.6898790198779636 1103 90-91 0.7254468931397327 1103 92-93 0.6914436546936109 1103 94-95 0.7415304564623959 1103 96-97 0.8041159031747362 1103 98-99 0.755266946481413 1103 100 0.8383104539550175 1104 1 0.2895644249349303 1104 2 0.20200629746858567 1104 3 0.25839650703920825 1104 4 0.19199250037820548 1104 5 0.2269716059980169 1104 6 0.21311449504626268 1104 7 0.2271333941200524 1104 8 0.2114728691285208 1104 9 0.26793727992419036 1104 10-11 0.2722309550784061 1104 12-13 0.28396754544246505 1104 14-15 0.34613838315035395 1104 16-17 0.3738396800620549 1104 18-19 0.35336746603726965 1104 20-21 0.344352298014293 1104 22-23 0.35827981120245767 1104 24-25 0.3727246788277796 1104 26-27 0.3481399642339298 1104 28-29 0.3668954686590027 1104 30-31 0.3967139908676387 1104 32-33 0.3607123873264797 1104 34-35 0.3831782637237282 1104 36-37 0.3944480249694422 1104 38-39 0.44920705761000335 1104 40-41 0.4364097941615128 1104 42-43 0.46217860695153945 1104 44-45 0.3755080082151565 1104 46-47 0.4282303313021032 1104 48-49 0.5008590717072678 1104 50-51 0.5811372036034896 1104 52-53 0.5101018562885145 1104 54-55 0.5465985817242043 1104 56-57 0.42595760587763465 1104 58-59 0.553670984417046 1104 60-61 0.5985806314024273 1104 62-63 0.572751642046633 1104 64-65 0.5494615540371655 1104 66-67 0.5766121471665855 1104 68-69 0.5125308220523266 1104 70-71 0.5161361576590267 1104 72-73 0.564508917368542 1104 74-75 0.5671284216126864 1104 76-77 0.44319775463276656 1104 78-79 0.5826817802789961 1104 80-81 0.5889097282274705 1104 82-83 0.5903796188757653 1104 84-85 0.5901590125146825 1104 86-87 0.6028180435670478 1104 88-89 0.5865070167211499 1104 90-91 0.5444197239477901 1104 92-93 0.5804608282942461 1104 94-95 0.6763560202458443 1104 96-97 0.7187923379864323 1104 98-99 0.6549484667439316 1104 100 0.692748529112432 1105 1 0.2243661504791845 1105 2 0.1545463840491763 1105 3 0.1763122185904038 1105 4 0.16476221181715545 1105 5 0.20651610434676115 1105 6 0.1850307562585698 1105 7 0.2139664870283582 1105 8 0.2006904928688087 1105 9 0.23394351208278863 1105 10-11 0.2398350104355913 1105 12-13 0.2614153748671484 1105 14-15 0.2934246968783256 1105 16-17 0.2932112489032548 1105 18-19 0.27758235457230995 1105 20-21 0.3002540600877026 1105 22-23 0.3609388278441159 1105 24-25 0.3188988458312636 1105 26-27 0.26681521752828274 1105 28-29 0.3422610394922714 1105 30-31 0.3013937774181983 1105 32-33 0.35020118732636973 1105 34-35 0.3637048021094742 1105 36-37 0.3687886695125897 1105 38-39 0.36578226348125753 1105 40-41 0.3130462460149417 1105 42-43 0.32440534670961796 1105 44-45 0.3215084927884462 1105 46-47 0.40293337157891784 1105 48-49 0.3151028512355225 1105 50-51 0.3066764223924636 1105 52-53 0.3033894957670853 1105 54-55 0.38520195986470895 1105 56-57 0.41870431318557877 1105 58-59 0.32341641520394404 1105 60-61 0.40906607207108436 1105 62-63 0.3474824273849393 1105 64-65 0.3428473768347473 1105 66-67 0.35705590543097543 1105 68-69 0.3358607644758038 1105 70-71 0.3664341067997938 1105 72-73 0.31447771796415225 1105 74-75 0.35264461696495175 1105 76-77 0.28781713637846806 1105 78-79 0.3238217354980222 1105 80-81 0.3111003718260221 1105 82-83 0.3550721251304694 1105 84-85 0.3307477897476261 1105 86-87 0.2786030033442941 1105 88-89 0.3271757057987088 1105 90-91 0.35322143101372205 1105 92-93 0.3630527603557958 1105 94-95 0.36605012266897674 1105 96-97 0.4239376265454098 1105 98-99 0.4034373468625141 1105 100 0.4316644666783205 1106 1 0.13339097175548886 1106 2 0.0979982689096559 1106 3 0.14765377114677136 1106 4 0.09931522087457978 1106 5 0.10342924413230747 1106 6 0.09405394604559092 1106 7 0.14243261121567485 1106 8 0.13242667151944687 1106 9 0.14803517080052586 1106 10-11 0.18089650685772796 1106 12-13 0.14063884462873233 1106 14-15 0.20979973483565573 1106 16-17 0.17492468294346963 1106 18-19 0.1880290737527659 1106 20-21 0.18522696224224688 1106 22-23 0.20910274705533283 1106 24-25 0.218688521198807 1106 26-27 0.2431011358418118 1106 28-29 0.23623812183399906 1106 30-31 0.22587163439654034 1106 32-33 0.21517138010882064 1106 34-35 0.17052797442672585 1106 36-37 0.1914658297716727 1106 38-39 0.16455927895025013 1106 40-41 0.22316815217735808 1106 42-43 0.20393011037969444 1106 44-45 0.2125382805747691 1106 46-47 0.16508634899644647 1106 48-49 0.19286998767028507 1106 50-51 0.16581084216934272 1106 52-53 0.21800231448837337 1106 54-55 0.19677904447951988 1106 56-57 0.1917657579133376 1106 58-59 0.13451273292346855 1106 60-61 0.09955602938381247 1106 62-63 0.0754511764826944 1106 64-65 0.15991632018055668 1106 66-67 0.11069182136601796 1106 68-69 0.0919340062459284 1106 70-71 0.12593475009234822 1106 72-73 0.1533809373782944 1106 74-75 0.11430194243890668 1106 76-77 0.021759464332202327 1106 78-79 0.17808847867970456 1106 80-81 0.22876352556629342 1106 82-83 0.2156525393582669 1106 84-85 0.17387754713141845 1106 86-87 0.20690861831631224 1106 88-89 0.1641608895846005 1106 90-91 0.21460907497546344 1106 92-93 0.2523700343351827 1106 94-95 0.2024564577235317 1106 96-97 0.2656595644943742 1106 98-99 0.296333650045284 1106 100 0.3741120140687286 1107 1 0.0960567112305668 1107 2 0.050948193314546586 1107 3 0.11972436996154556 1107 4 0.07284351197596806 1107 5 0.11810525131715366 1107 6 0.10680069101786671 1107 7 0.04874909508733083 1107 8 0.07755190515175059 1107 9 0.08820472097292509 1107 10-11 0.13352262144581317 1107 12-13 0.16126064469942492 1107 14-15 0.20033469821564864 1107 16-17 0.1442907430988285 1107 18-19 0.1566798137291059 1107 20-21 0.17763593471276096 1107 22-23 0.15600377182835956 1107 24-25 0.19516294413077873 1107 26-27 0.18870556875116762 1107 28-29 0.18455851532817036 1107 30-31 0.2398103616015277 1107 32-33 0.2327110991471386 1107 34-35 0.2439435314780951 1107 36-37 0.231734278612322 1107 38-39 0.21154900929556675 1107 40-41 0.1596704774939468 1107 42-43 0.21221346394892748 1107 44-45 0.18488354486945724 1107 46-47 0.2553383987104638 1107 48-49 0.2782925166931989 1107 50-51 0.29783053031541584 1107 52-53 0.3211711827046386 1107 54-55 0.3260920192032941 1107 56-57 0.37840399706971084 1107 58-59 0.43482671210480106 1107 60-61 0.35792256321313687 1107 62-63 0.3881339162383668 1107 64-65 0.3309066983576727 1107 66-67 0.3664832414490533 1107 68-69 0.3143635381821426 1107 70-71 0.34891247256395985 1107 72-73 0.32510078937418285 1107 74-75 0.344850403493588 1107 76-77 0.26764148008632205 1107 78-79 0.32564068580766303 1107 80-81 0.30129878103640806 1107 82-83 0.3132926917755512 1107 84-85 0.33529456604779284 1107 86-87 0.3365712436370174 1107 88-89 0.3755548436376941 1107 90-91 0.39642666046177055 1107 92-93 0.34950107122499574 1107 94-95 0.40205524933333336 1107 96-97 0.45736641565165925 1107 98-99 0.45198098766696404 1107 100 0.4475711547827821 1108 1 0.06823628893769751 1108 2 0.03344146970626127 1108 3 0.037882294471842215 1108 4 0.036102858037288854 1108 5 0.06521938627290069 1108 6 0.020981470187862783 1108 7 0.02624988200673073 1108 8 0.05090947315606087 1108 9 0.0883483259735911 1108 10-11 0.11432213432434679 1108 12-13 0.08172021324463685 1108 14-15 0.082784571138383 1108 16-17 0.07943560204672195 1108 18-19 0.11871077964791255 1108 20-21 0.14514995974212042 1108 22-23 0.07632425332193549 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5 134.0 6 298.0 7 652.0 8 1482.0 9 2700.0 10 4643.0 11 8177.0 12 13108.0 13 18313.0 14 21111.0 15 27387.0 16 36257.0 17 48527.0 18 49380.0 19 31486.0 20 22518.0 21 19970.0 22 21197.0 23 24194.0 24 27885.0 25 32719.0 26 40389.0 27 52597.0 28 67227.0 29 85902.0 30 108736.0 31 137933.0 32 176611.0 33 233519.0 34 335238.0 35 547859.0 36 1058996.0 37 2006271.0 38 1864990.0 39 350868.0 40 319.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.16485349428079 19.09300470018348 13.785110327836328 23.9570314776994 2 15.40665155699131 22.40146716000087 36.43255588336886 25.75932539963896 3 19.105616146789774 26.833695382971136 29.75652184146238 24.304166628776713 4 13.874526855109028 18.496806480898385 35.21628621204646 32.41238045194613 5 13.985171613651229 37.32562821971125 33.197726435310045 15.49147373132748 6 31.499558538916222 34.8921107615431 17.887850702193187 15.72047999734749 7 26.104441510283554 32.40688005467593 22.34492309058054 19.14375534445997 8 27.491206875990347 31.86365029223066 21.179556742054636 19.465586089724358 9 27.743449343209655 14.759947848231509 19.77948871800878 37.717114090550055 10-11 25.2401399467706 25.70904238017001 26.331700959704136 22.719116713355252 12-13 26.985009479332273 23.62910325135649 26.54311302996573 22.842774239345502 14-15 25.27240609985377 23.542139962398164 24.93741215792772 26.248041779820348 16-17 23.55574941424594 26.479539552186427 26.74973177310512 23.21497926046252 18-19 24.578051710040015 25.72260842268174 28.006534479688245 21.69280538759 20-21 24.71408981667936 25.397632365466606 27.178649401319515 22.70962841653452 22-23 23.644330673272595 25.911268513580843 25.624652333985864 24.819748479160697 24-25 23.735433218668824 26.617688416157097 25.253091840637122 24.393786524536957 26-27 24.372193518874855 25.503392628033833 25.734338537638042 24.390075315453274 28-29 23.80431993029304 24.89997573974232 27.57763566574753 23.71806866421711 30-31 25.05496177460134 25.037440588572807 26.683048295969396 23.224549340856456 32-33 22.867459101614262 25.850845455799167 26.99094584479701 24.290749597789564 34-35 22.960856288779336 26.295609265200003 27.2320790226242 23.511455423396463 36-37 24.354974749701398 26.167495820028208 26.101976003074572 23.375553427195825 38-39 24.596286429449506 25.14429121405438 26.097107584285943 24.162314772210173 40-41 23.760970305588934 25.934582531766644 26.197971475300292 24.106475687344133 42-43 25.44302589106881 25.239436868284088 25.927260927793718 23.390276312853388 44-45 25.114359793055403 25.096250736829358 26.3122982618381 23.47709120827714 46-47 24.138155554585502 25.35401005501063 27.347471029190306 23.16036336121356 48-49 24.696694274928337 26.039688327301118 26.663075170539802 22.60054222723074 50-51 23.560620630565605 26.677390521521477 25.63088881985655 24.131100028056363 52-53 23.241660766421905 26.778077282097794 24.641728514223328 25.33853343725697 54-55 24.041769776040223 26.167437454779964 24.552833918260493 25.237958850919323 56-57 24.602965150049112 25.086583448578452 24.915954028011736 25.394497373360696 58-59 24.125380655572425 25.07084875887043 26.442101470841834 24.361669114715312 60-61 24.29918015111858 25.56027575914503 25.756239801200937 24.384304288535454 62-63 22.63760486414116 26.020740482045966 26.2444493127988 25.097205341014075 64-65 22.70308299929144 26.015524466333517 26.038394478359493 25.24299805601555 66-67 24.109096419767358 25.845450526695906 25.228821146529295 24.816631907007444 68-69 24.281083311290054 25.087162028420085 25.275701409078515 25.356053251211346 70-71 23.32139835150988 25.546910815724654 25.59459325402794 25.537097578737523 72-73 24.471475867383617 25.068856840742125 25.558939998387626 24.900727293486632 74-75 24.58993116155492 24.74623540484644 25.772223918379783 24.891609515218857 76-77 23.408279158380267 25.62401189899226 26.467503384193726 24.500205558433745 78-79 23.573230087356787 25.562671119303154 26.287508037681356 24.576590755658696 80-81 22.83664683166776 25.67798769684592 25.98313329878653 25.50223217269979 82-83 22.821752705324066 26.092628178260263 24.895913593274937 26.189705523140734 84-85 23.14853375941063 25.79278172259956 24.73628980386531 26.3223947141245 86-87 23.2263318333323 24.789764884413554 25.389718505309368 26.594184776944786 88-89 23.210544160571057 24.3984454465043 26.131869656874123 26.25914073605052 90-91 23.57048783054112 24.890787250288014 25.656282559103232 25.882442360067635 92-93 22.468659943085534 25.267937181510618 25.96765621897831 26.29574665642554 94-95 21.89339484649663 25.526360630791135 26.341177857106235 26.239066665606003 96-97 23.00844746616458 25.142998745203432 25.514611317555115 26.333942471076877 98-99 23.040030439690963 24.706325434094488 25.44815252146207 26.805491604752486 100 23.0101953913911 25.196312556703827 25.665759405448433 26.127732646456643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 436.0 1 308.0 2 233.0 3 300.0 4 279.0 5 339.5 6 560.0 7 1128.0 8 1884.0 9 2072.5 10 1753.5 11 1599.0 12 1767.5 13 2027.5 14 2333.5 15 2758.5 16 3172.0 17 3585.0 18 4135.0 19 4720.0 20 5495.5 21 6506.0 22 7863.5 23 9548.5 24 11498.5 25 14331.5 26 18572.0 27 23912.5 28 29229.5 29 36997.5 30 46539.0 31 55187.5 32 64977.5 33 75881.0 34 87600.0 35 97027.5 36 105200.5 37 116865.0 38 126640.0 39 135319.0 40 142284.0 41 147409.5 42 155540.5 43 172619.0 44 195495.5 45 216630.0 46 245007.5 47 279179.5 48 374376.0 49 476902.0 50 572295.0 51 539121.0 52 379983.0 53 315437.0 54 290514.5 55 262779.5 56 240776.5 57 217125.0 58 202630.5 59 186306.0 60 157356.5 61 129022.0 62 104944.5 63 81902.5 64 60342.0 65 47100.0 66 36356.0 67 26724.0 68 20395.0 69 16569.0 70 14050.0 71 11974.5 72 10234.5 73 10424.0 74 8457.0 75 6229.0 76 4654.5 77 3172.5 78 2256.0 79 1377.5 80 928.5 81 749.5 82 577.0 83 425.5 84 297.0 85 178.0 86 106.0 87 53.5 88 25.5 89 17.5 90 12.0 91 7.0 92 3.5 93 2.5 94 2.0 95 2.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06131255391253241 3 0.025562495220401653 4 0.006658013922468631 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.358654107887635E-5 12-13 1.9385783509195812E-4 14-15 2.7407487030242357E-4 16-17 4.411936936575599E-4 18-19 0.0 20-21 0.0029078675263793717 22-23 0.004913293406641008 24-25 8.222246109072707E-4 26-27 4.612479524601763E-4 28-29 0.005314378582693335 30-31 0.0025602603738006883 32-33 0.0013837438573805288 34-35 0.0017179815040908014 36-37 0.003897210960641779 38-39 0.0040442755251942995 40-41 0.001136407998814927 42-43 0.002393141550445552 44-45 3.342376467102726E-4 46-47 0.002052219150801074 48-49 3.3423764671027265E-5 50-51 0.0016243949630119251 52-53 0.006664698675402836 54-55 0.013356136362542494 56-57 0.0241520123512843 58-59 0.02651841488999303 60-61 0.024138642845415892 62-63 0.007573825074454778 64-65 0.006437417075639851 66-67 1.671188233551363E-4 68-69 0.0013837438573805288 70-71 2.005425880261636E-4 72-73 7.888008462362435E-4 74-75 0.005140575006403993 76-77 0.0011096689870781053 78-79 0.0012634183045648307 80-81 0.0 82-83 0.001403798116183145 84-85 1.3369505868410905E-5 86-87 6.216820228811071E-4 88-89 5.347802347364362E-5 90-91 5.481497406048471E-4 92-93 8.42278869709887E-4 94-95 0.0015575474336698707 96-97 1.270103057499036E-4 98-99 0.0 100 7.219533168941888E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7479708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.3494258614592 #Duplication Level Percentage of deduplicated Percentage of total 1 78.71659397728821 31.761693727531764 2 12.07299877335793 9.742771378621875 3 3.517361424596944 4.257705420891927 4 1.6207500521281317 2.6158533627320075 5 0.9009542303794511 1.817649296163185 6 0.5766594324452691 1.3960726210056915 7 0.3977585091805359 1.12345292338612 8 0.2909837752876609 0.9392822614285583 9 0.22057391950407393 0.801002791080099 >10 1.4006562737975337 10.685616150667299 >50 0.14079739749414877 3.965431246061385 >100 0.11742641068295515 9.888066042343656 >500 0.016237588646800753 4.473458099323611 >1k 0.008684381136217664 6.226688038224274 >5k 7.985637825336027E-4 2.3350717860472017 >10k+ 7.652902915947026E-4 7.970184854491446 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 79018 1.056431614710093 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 71174 0.9515612106782778 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 39192 0.5239776739947603 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 36726 0.49100847252325897 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 34733 0.464363047327516 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 34354 0.45929600460338826 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 31880 0.4262198470849397 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 26514 0.35447907859504674 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 26096 0.348890625142051 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 24639 0.3294112550917763 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 24614 0.329077017445066 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 20779 0.2778049624397102 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17214 0.23014267401882532 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 16445 0.21986152400601736 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 15260 0.20401865955195042 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 14858 0.19864411819284924 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 12976 0.17348270814849992 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 12153 0.16247960481879775 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 11713 0.15659702223669694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11327 0.15143639297149034 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 11015 0.14726510714054614 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10946 0.14634261123562578 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10027 0.13405603534255617 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 9849 0.13167626329797902 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9833 0.13146235120408445 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 9361 0.1251519444341945 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 9323 0.12464390321119487 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 9078 0.1213683742734342 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 9003 0.12036566133330338 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 8817 0.11787893324177896 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 7725 0.10327943283347425 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 7481 0.10001727340158198 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0024599890797876067 0.0 0.0 0.0 1.3369505868410905E-5 2 0.0039172652194443955 0.0 0.0 1.3369505868410905E-5 1.3369505868410905E-5 3 0.004091068795733737 0.0 0.0 1.3369505868410905E-5 1.3369505868410905E-5 4 0.004732805077417461 0.0 0.0 2.673901173682181E-5 1.3369505868410905E-5 5 0.018931220309669844 0.0 0.0 1.7380357628934178E-4 1.3369505868410905E-5 6 0.0391058046651019 0.0 0.0 4.679327053943817E-4 1.3369505868410905E-5 7 0.043731653695572074 0.0 0.0 4.946717171312035E-4 1.3369505868410905E-5 8 0.05123194648775059 0.0 0.0 5.214107288680253E-4 1.3369505868410905E-5 9 0.05623214168253627 0.0 0.0 6.016277640784908E-4 1.3369505868410905E-5 10-11 0.1057995846896697 0.0 0.0 0.001123038492946516 1.3369505868410905E-5 12-13 0.18404461778454453 0.0 0.0 0.0025134671032612504 4.010851760523272E-5 14-15 0.2742286196199103 6.684752934205453E-6 0.0 0.004184655336812613 7.353228227625998E-5 16-17 0.3143304524722088 1.3369505868410905E-5 0.0 0.004899923900772597 8.021703521046544E-5 18-19 0.33263330600606333 1.3369505868410905E-5 0.0 0.0054213346296406225 8.021703521046544E-5 20-21 0.39700747676246184 1.3369505868410905E-5 0.0 0.007132631380797219 8.021703521046544E-5 22-23 0.41476218055571157 1.3369505868410905E-5 0.0 0.00854311424991457 8.690178814467089E-5 24-25 0.4987093079034636 1.3369505868410905E-5 0.0 0.01527466045465946 9.358654107887635E-5 26-27 0.6669311154927439 1.3369505868410905E-5 0.0 0.03875819751252321 1.0027129401308179E-4 28-29 0.7057361062758065 1.3369505868410905E-5 0.0 0.05850495768016613 1.0695604694728724E-4 30-31 0.718443821603731 1.3369505868410905E-5 0.0 0.1045695901497759 1.0695604694728724E-4 32-33 0.7306969737321296 1.3369505868410905E-5 0.0 0.17764730922650993 1.0695604694728724E-4 34-35 0.7399954650636095 1.3369505868410905E-5 0.0 0.2765549136410138 1.2032555281569815E-4 36-37 0.755042843918506 1.3369505868410905E-5 0.0 0.4239002378167704 1.2032555281569815E-4 38-39 0.7706717962786782 1.3369505868410905E-5 0.0 0.6452925702447208 1.2032555281569815E-4 40-41 0.7848234182403913 1.3369505868410905E-5 0.0 0.9445355353444278 1.2032555281569815E-4 42-43 0.8131132926579487 2.673901173682181E-5 0.0 1.1753333151508052 1.2032555281569815E-4 44-45 0.8451934219892007 2.673901173682181E-5 0.0 1.4215193962117239 1.3369505868410906E-4 46-47 0.8576203776938887 2.673901173682181E-5 0.0 1.7019648360604451 1.5374931748672542E-4 48-49 0.8662102852143425 2.673901173682181E-5 0.0 1.9615003687309718 1.6043407042093088E-4 50-51 0.8807295685874368 2.673901173682181E-5 0.0 2.2456759007169795 1.6043407042093088E-4 52-53 0.8858032960644988 2.673901173682181E-5 0.0 2.5731218384460997 1.7380357628934178E-4 54-55 0.9011982820719739 4.010851760523272E-5 0.0 2.8735747438268984 2.1391209389457448E-4 56-57 0.931199453240688 4.010851760523272E-5 0.0 3.193767724622405 2.1391209389457448E-4 58-59 0.9410594638186411 4.010851760523272E-5 0.0 3.5259330979230743 2.2059684682877994E-4 60-61 0.9477442167528465 4.010851760523272E-5 0.0 3.83326461407317 2.272815997629854E-4 62-63 0.9533727787234475 4.010851760523272E-5 0.0 4.1508037479537965 2.272815997629854E-4 64-65 0.9583930281770358 5.347802347364362E-5 0.0 4.497849381286008 2.272815997629854E-4 66-67 0.9655523985695699 5.347802347364362E-5 0.0 4.862068144906191 2.406511056313963E-4 68-69 0.9735473630788796 5.347802347364362E-5 0.0 5.290453851941814 2.406511056313963E-4 70-71 0.9829594952102408 5.347802347364362E-5 0.0 5.7889893562689885 2.406511056313963E-4 72-73 1.005614122904263 6.0162776407849075E-5 0.0 6.21136547041676 2.9412912910503993E-4 74-75 1.0276136448107334 6.684752934205453E-5 0.0 6.62356739059867 3.0749863497345084E-4 76-77 1.0343251367566757 6.684752934205453E-5 0.0 7.079534388240824 3.0749863497345084E-4 78-79 1.0392985929397245 6.684752934205453E-5 0.0 7.531463795110718 3.0749863497345084E-4 80-81 1.0487775726004278 6.684752934205453E-5 0.0 8.038361925358583 3.141833879076563E-4 82-83 1.052821848125622 6.684752934205453E-5 0.0 8.608885534034215 3.2086814084186175E-4 84-85 1.0675416740867423 6.684752934205453E-5 0.0 9.144842285287073 3.2086814084186175E-4 86-87 1.0916402084145531 6.684752934205453E-5 0.0 9.686367435734123 3.275528937760672E-4 88 1.0976765403141406 6.684752934205453E-5 0.0 10.119338883282609 3.4760715257868357E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 1930 0.0 24.112194 3 AGTACTT 20930 0.0 18.915854 12-13 TCTACAC 4075 0.0 18.802639 3 GATCTAC 3090 0.0 18.56992 1 CGATCTC 2255 0.0 18.338154 5 TACACTC 3580 0.0 17.589005 5 GAGTACT 19870 0.0 16.897778 12-13 TACTTTT 24400 0.0 16.438612 14-15 CTACACT 5615 0.0 16.236692 4 TAGTACT 2370 0.0 16.06136 4 ATCTACA 4010 0.0 15.71698 2 CATGGGG 16080 0.0 15.606332 4 GTATAAG 2660 0.0 15.383185 1 CGTTAAC 740 0.0 15.254156 1 TCTCGCG 925 0.0 15.240696 8 GTACTTT 22390 0.0 15.175241 14-15 CCGATCT 3115 0.0 15.086466 4 TATACCG 1050 0.0 14.321224 5 TCTAACG 1095 0.0 14.174524 2 GTATATG 2165 0.0 14.120955 1 >>END_MODULE