##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765309_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8575597 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18389238673412 33.0 31.0 34.0 31.0 34.0 2 32.175645147504014 34.0 31.0 34.0 30.0 34.0 3 32.19967880953361 34.0 31.0 34.0 30.0 34.0 4 35.667102710167 37.0 35.0 37.0 35.0 37.0 5 35.69572742282549 37.0 35.0 37.0 35.0 37.0 6 35.80673916929632 37.0 36.0 37.0 35.0 37.0 7 35.765990519377254 37.0 36.0 37.0 35.0 37.0 8 35.8057014572863 37.0 36.0 37.0 35.0 37.0 9 37.485539840549876 39.0 38.0 39.0 35.0 39.0 10-11 37.41883567989494 39.0 38.0 39.0 35.0 39.0 12-13 37.4018373881142 39.0 38.0 39.0 35.0 39.0 14-15 38.76509034881187 41.0 38.5 41.0 35.0 41.0 16-17 38.7092790157933 40.0 38.0 41.0 35.0 41.0 18-19 38.686747814758554 40.0 38.5 41.0 34.5 41.0 20-21 38.67663166774278 40.0 39.0 41.0 34.5 41.0 22-23 38.59390955521813 40.0 38.5 41.0 34.0 41.0 24-25 38.521783556293514 40.0 38.0 41.0 34.0 41.0 26-27 38.39372063542632 40.0 38.0 41.0 34.0 41.0 28-29 38.25610934142544 40.0 38.0 41.0 34.0 41.0 30-31 38.0396367739762 40.0 38.0 41.0 33.5 41.0 32-33 37.767062048274894 40.0 37.5 41.0 33.0 41.0 34-35 37.654858956175296 40.0 37.0 41.0 33.0 41.0 36-37 37.53130289354782 40.0 37.0 41.0 33.0 41.0 38-39 37.35401494496534 40.0 37.0 41.0 32.0 41.0 40-41 37.120241307981246 40.0 37.0 41.0 31.0 41.0 42-43 36.891975509110324 40.0 36.0 41.0 31.0 41.0 44-45 36.56501115898986 40.0 36.0 41.0 30.5 41.0 46-47 36.2000229255176 39.0 35.0 41.0 30.0 41.0 48-49 35.85711851897891 39.0 35.0 41.0 28.5 41.0 50-51 34.631158273878775 38.0 34.0 40.0 26.0 40.5 52-53 34.59332673865155 38.0 34.0 39.5 26.0 40.0 54-55 35.42849319994865 39.0 35.0 40.5 28.0 41.0 56-57 35.45647072734411 39.0 35.0 41.0 28.0 41.0 58-59 35.26056016858068 38.5 35.0 41.0 28.0 41.0 60-61 34.90484493382793 38.0 35.0 40.0 27.0 41.0 62-63 34.46207698426127 37.0 34.5 40.0 26.0 41.0 64-65 34.20561361500546 37.0 34.0 39.5 26.0 41.0 66-67 33.922115917993814 36.0 34.0 39.0 26.0 41.0 68-69 33.6934986567116 36.0 34.0 39.0 26.0 41.0 70-71 33.32152105561863 35.0 34.0 38.5 26.0 40.5 72-73 32.94022596910746 35.0 34.0 37.0 25.0 39.5 74-75 32.55701620540238 35.0 34.0 37.0 24.0 39.0 76-77 32.17613385983506 35.0 34.0 36.5 23.0 39.0 78-79 31.867572368431027 35.0 33.5 36.0 22.0 37.0 80-81 31.55249302176863 35.0 33.0 35.5 20.0 37.0 82-83 31.28072786069588 35.0 33.0 35.0 19.5 36.5 84-85 31.013638234166088 35.0 33.0 35.0 17.5 36.0 86-87 30.738360722874454 35.0 33.0 35.0 9.5 36.0 88-89 30.50921883339434 35.0 33.0 35.0 4.5 36.0 90-91 30.26150435940495 35.0 32.0 35.0 2.0 35.0 92-93 29.968681422412924 35.0 32.0 35.0 2.0 35.0 94-95 29.671346962782884 35.0 32.0 35.0 2.0 35.0 96-97 29.392647415684294 35.0 31.5 35.0 2.0 35.0 98-99 29.069074374647037 35.0 31.0 35.0 2.0 35.0 100 28.517849894298905 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4712069820922622 1101 2 0.5771235463328495 1101 3 0.6286701100045562 1101 4 0.49259117255741813 1101 5 0.5517498971164088 1101 6 0.5319187584194296 1101 7 0.6094089210716902 1101 8 0.5442637670230042 1101 9 0.665177050290076 1101 10-11 0.7033817782140019 1101 12-13 0.7038898642770022 1101 14-15 0.8867914235099903 1101 16-17 0.8747709910700436 1101 18-19 0.8485587946416331 1101 20-21 0.8671961429138051 1101 22-23 0.8263131422981687 1101 24-25 0.9205707788072459 1101 26-27 0.8926781942912854 1101 28-29 0.9229390480355448 1101 30-31 0.9904045969212305 1101 32-33 1.01725910128296 1101 34-35 1.107247900821669 1101 36-37 1.134956749099132 1101 38-39 1.0798374056180364 1101 40-41 1.0818454454343183 1101 42-43 1.0538896837756084 1101 44-45 1.1674173662073812 1101 46-47 1.1781443822595108 1101 48-49 1.229070801701468 1101 50-51 1.2469910384508438 1101 52-53 1.3131091264965633 1101 54-55 1.4883619555186414 1101 56-57 1.5416666837910853 1101 58-59 1.4750964606828134 1101 60-61 1.455357609444171 1101 62-63 1.5081264962334942 1101 64-65 1.4575768044376431 1101 66-67 1.4597740181402514 1101 68-69 1.443112889603043 1101 70-71 1.4087862332978602 1101 72-73 1.3835822971758844 1101 74-75 1.3474942377845522 1101 76-77 1.2967685746468476 1101 78-79 1.3048082333921975 1101 80-81 1.340923282599345 1101 82-83 1.334106539158043 1101 84-85 1.4058784614110813 1101 86-87 1.4114275053480014 1101 88-89 1.3818941793364452 1101 90-91 1.3676636211373996 1101 92-93 1.379162442275458 1101 94-95 1.3925423835300599 1101 96-97 1.2620450727015111 1101 98-99 1.2769245806501814 1101 100 1.3512774658899502 1102 1 0.4345818723439905 1102 2 0.5253783289987624 1102 3 0.607895754960218 1102 4 0.49648700675208346 1102 5 0.4627059274316636 1102 6 0.4836062972927735 1102 7 0.49616619964543673 1102 8 0.5177434043144373 1102 9 0.6587585880675633 1102 10-11 0.6307183120949276 1102 12-13 0.6239138355506952 1102 14-15 0.7975821968916961 1102 16-17 0.8399366417862026 1102 18-19 0.8183554088369291 1102 20-21 0.8268531463754201 1102 22-23 0.8461206238568124 1102 24-25 0.8577538113429384 1102 26-27 0.8600506909149743 1102 28-29 0.9379539489165936 1102 30-31 0.9544359031359662 1102 32-33 0.9479365879640156 1102 34-35 0.952364039476187 1102 36-37 0.9521570376512756 1102 38-39 0.9754173696475164 1102 40-41 1.0371022143803472 1102 42-43 1.0169460162040025 1102 44-45 1.0122083803948314 1102 46-47 0.990757729766429 1102 48-49 0.9410705957162193 1102 50-51 0.9887417030751138 1102 52-53 1.0449379488696664 1102 54-55 1.1607517221993078 1102 56-57 1.167276209813771 1102 58-59 1.1971157325724988 1102 60-61 1.179364327898334 1102 62-63 1.1885218224421052 1102 64-65 1.1107820782820212 1102 66-67 1.10046761081108 1102 68-69 1.0491140070557137 1102 70-71 0.9924224422724848 1102 72-73 1.0267026103149277 1102 74-75 0.9751917405029005 1102 76-77 0.9058853809389404 1102 78-79 0.961191255179326 1102 80-81 0.8863432127691446 1102 82-83 0.8833760346052273 1102 84-85 0.9151027921739576 1102 86-87 0.8941653434594095 1102 88-89 0.8935244397958897 1102 90-91 0.9013436883798285 1102 92-93 0.9302980849850648 1102 94-95 0.8744122042248286 1102 96-97 0.9510168892282778 1102 98-99 0.9584948284662431 1102 100 0.9689146760185317 1103 1 0.4865763452451475 1103 2 0.5463930698524848 1103 3 0.582101384772507 1103 4 0.5047841066681542 1103 5 0.5113407747303711 1103 6 0.5098904317204358 1103 7 0.5315474383490439 1103 8 0.5024717522629558 1103 9 0.6064430955848152 1103 10-11 0.6038198685690475 1103 12-13 0.6401186523751932 1103 14-15 0.7791122705478841 1103 16-17 0.7786231239084884 1103 18-19 0.8069803518540439 1103 20-21 0.8141309572262472 1103 22-23 0.8034009760959648 1103 24-25 0.8351960446486331 1103 26-27 0.8244475515830914 1103 28-29 0.8547189330896217 1103 30-31 0.908549798527261 1103 32-33 0.9899244745740177 1103 34-35 1.016185004399098 1103 36-37 1.0077870142362002 1103 38-39 0.9439216860805715 1103 40-41 1.030031709007126 1103 42-43 1.0082922523525895 1103 44-45 0.9598320580874926 1103 46-47 0.9254371774284138 1103 48-49 1.0466816301021424 1103 50-51 1.0022113617356396 1103 52-53 0.9804237122863739 1103 54-55 1.078811163436896 1103 56-57 1.1419412490622278 1103 58-59 1.1465082237517663 1103 60-61 1.1391810193457417 1103 62-63 1.2016274438552301 1103 64-65 1.1821236612295465 1103 66-67 1.0746725994657922 1103 68-69 1.047443290677542 1103 70-71 1.050997616234561 1103 72-73 0.9508425695629512 1103 74-75 0.981528781650475 1103 76-77 1.022654981446479 1103 78-79 0.9828527637333302 1103 80-81 0.9467221833778403 1103 82-83 0.9590935179784026 1103 84-85 0.9338399209357071 1103 86-87 0.9347097439418164 1103 88-89 0.9942680062605298 1103 90-91 0.9487522016059486 1103 92-93 0.9232478161476969 1103 94-95 1.0209951509401485 1103 96-97 1.0752954066347442 1103 98-99 1.1097717899435722 1103 100 1.1451255341502353 1104 1 0.27154301021698757 1104 2 0.36521724479200657 1104 3 0.4161732897099455 1104 4 0.3419141058631183 1104 5 0.3799109382383179 1104 6 0.3294599575516344 1104 7 0.37637664267253257 1104 8 0.35447365470053427 1104 9 0.4283627567899231 1104 10-11 0.48619695161107757 1104 12-13 0.44023988510389245 1104 14-15 0.5468357381111133 1104 16-17 0.5244946172160638 1104 18-19 0.5478921342558678 1104 20-21 0.49521121022078063 1104 22-23 0.4710015425901801 1104 24-25 0.5424216000205817 1104 26-27 0.5470384946667437 1104 28-29 0.5790496638120786 1104 30-31 0.5566426207446824 1104 32-33 0.5777335147540015 1104 34-35 0.5434002160611655 1104 36-37 0.5430424080068832 1104 38-39 0.49074672855704904 1104 40-41 0.48165918821377574 1104 42-43 0.5072691360412023 1104 44-45 0.5310484741323691 1104 46-47 0.5098765144564652 1104 48-49 0.5358180865685256 1104 50-51 0.4518919672531325 1104 52-53 0.46661493904083073 1104 54-55 0.5719905910531722 1104 56-57 0.5667598766378248 1104 58-59 0.5787249077903596 1104 60-61 0.5273233568441 1104 62-63 0.5010387580624993 1104 64-65 0.5550473719997129 1104 66-67 0.5542989865715953 1104 68-69 0.4858011088909464 1104 70-71 0.4818810580364925 1104 72-73 0.4711913956869367 1104 74-75 0.44688302061188523 1104 76-77 0.34640226973144195 1104 78-79 0.327455815260155 1104 80-81 0.41124283478263024 1104 82-83 0.42417994859875563 1104 84-85 0.3769753891090737 1104 86-87 0.3369117412894198 1104 88-89 0.3714744525156064 1104 90-91 0.3190824906154539 1104 92-93 0.36429923598473835 1104 94-95 0.3546605369570166 1104 96-97 0.3702103848350973 1104 98-99 0.44316274722135063 1104 100 0.4569366420129377 1105 1 0.24811366212375674 1105 2 0.30440295263910855 1105 3 0.3626246759709133 1105 4 0.30611457776406326 1105 5 0.3056990306143703 1105 6 0.2834023029120374 1105 7 0.3209585191016515 1105 8 0.2767408896803758 1105 9 0.3511163490627709 1105 10-11 0.3647127661411673 1105 12-13 0.38359231351537915 1105 14-15 0.4908165315528805 1105 16-17 0.4770161482748989 1105 18-19 0.4808256210271722 1105 20-21 0.41765226630866437 1105 22-23 0.35001672826133756 1105 24-25 0.4191102310837138 1105 26-27 0.3888215627566822 1105 28-29 0.38109261060825617 1105 30-31 0.37074867560996694 1105 32-33 0.36806437459384256 1105 34-35 0.363508202777318 1105 36-37 0.39650281764676265 1105 38-39 0.39997944226658433 1105 40-41 0.4419029564032755 1105 42-43 0.4959557510554333 1105 44-45 0.46569637859316515 1105 46-47 0.47925449877288884 1105 48-49 0.4716714967023776 1105 50-51 0.37999457250791835 1105 52-53 0.3959411831196249 1105 54-55 0.4574102363035166 1105 56-57 0.5277688982920097 1105 58-59 0.5634250048375975 1105 60-61 0.582820229875118 1105 62-63 0.6792597305371899 1105 64-65 0.5677972976600643 1105 66-67 0.49486348810240344 1105 68-69 0.5443897037583341 1105 70-71 0.5204435985528733 1105 72-73 0.5275812303663798 1105 74-75 0.5209056786621318 1105 76-77 0.4929624548981195 1105 78-79 0.5415073295585664 1105 80-81 0.5046540724599531 1105 82-83 0.5829955723975715 1105 84-85 0.5663092627522914 1105 86-87 0.5103679990413781 1105 88-89 0.4952355988568762 1105 90-91 0.5609672627120084 1105 92-93 0.5687989307472847 1105 94-95 0.5656921250941274 1105 96-97 0.6290654421614903 1105 98-99 0.6971225750403001 1105 100 0.8033427306750305 1106 1 0.2377140356079792 1106 2 0.3261700710370974 1106 3 0.3309780911847895 1106 4 0.3086694445716418 1106 5 0.38084833928248685 1106 6 0.29812614637240387 1106 7 0.2886988556096597 1106 8 0.31691595785679283 1106 9 0.3328040181354268 1106 10-11 0.43933695931605854 1106 12-13 0.40381286158465457 1106 14-15 0.46930672726355027 1106 16-17 0.49456328755840673 1106 18-19 0.48014974273241506 1106 20-21 0.43749417044182337 1106 22-23 0.4959757735878938 1106 24-25 0.5393057322977839 1106 26-27 0.5408815607760005 1106 28-29 0.5211343217959694 1106 30-31 0.5700249732935916 1106 32-33 0.511517829443342 1106 34-35 0.5034689130775192 1106 36-37 0.4836230707951472 1106 38-39 0.4616869532360752 1106 40-41 0.4706027334264249 1106 42-43 0.4742231961499499 1106 44-45 0.4418358262297275 1106 46-47 0.4581069032454508 1106 48-49 0.4481237235115785 1106 50-51 0.39703007576835603 1106 52-53 0.41543055568495646 1106 54-55 0.37837570207613425 1106 56-57 0.4267167333263515 1106 58-59 0.3935977661052448 1106 60-61 0.42638927099873314 1106 62-63 0.4487032386032368 1106 64-65 0.35983009882313155 1106 66-67 0.3573682509823257 1106 68-69 0.33768964809622304 1106 70-71 0.34242878222872264 1106 72-73 0.36213202428673696 1106 74-75 0.4482695358311304 1106 76-77 0.47434108868996105 1106 78-79 0.4236399452129014 1106 80-81 0.4492023565861665 1106 82-83 0.3667819300206858 1106 84-85 0.4414925875603011 1106 86-87 0.4706874821887226 1106 88-89 0.43168078744137617 1106 90-91 0.4650534761478511 1106 92-93 0.46951179548207733 1106 94-95 0.4389935932616673 1106 96-97 0.46431241750923746 1106 98-99 0.5330443412756374 1106 100 0.6353278712924713 1107 1 0.21523611393863007 1107 2 0.32096842361198696 1107 3 0.32960999519978884 1107 4 0.2699618147149607 1107 5 0.29350909991558183 1107 6 0.23123586395617934 1107 7 0.33674595974412114 1107 8 0.24692284233919537 1107 9 0.3792226840564794 1107 10-11 0.38171481172481236 1107 12-13 0.4579230223115047 1107 14-15 0.4638288343517303 1107 16-17 0.4539296657143268 1107 18-19 0.4657445436489098 1107 20-21 0.43817184327195235 1107 22-23 0.5001912804081101 1107 24-25 0.5264574240891662 1107 26-27 0.4467945238717732 1107 28-29 0.4808058069073695 1107 30-31 0.5462975890954738 1107 32-33 0.5244192524558073 1107 34-35 0.5138192317143719 1107 36-37 0.5663832112011704 1107 38-39 0.5530872403262634 1107 40-41 0.5805661767209145 1107 42-43 0.5821025157122364 1107 44-45 0.5553060938930017 1107 46-47 0.540378893311285 1107 48-49 0.524437881811707 1107 50-51 0.33699534499250205 1107 52-53 0.4715696118352355 1107 54-55 0.5824397987901193 1107 56-57 0.5841704588291847 1107 58-59 0.5396402281513701 1107 60-61 0.5827231507250659 1107 62-63 0.5792943166327902 1107 64-65 0.5342790370641026 1107 66-67 0.5175410178824862 1107 68-69 0.5134577945281578 1107 70-71 0.4578319021084525 1107 72-73 0.504466220692322 1107 74-75 0.46894657807454365 1107 76-77 0.545304233668432 1107 78-79 0.5467660199388114 1107 80-81 0.5724959179147042 1107 82-83 0.5436483714085156 1107 84-85 0.5819271588185053 1107 86-87 0.558754217665566 1107 88-89 0.5537189614978608 1107 90-91 0.5721190616701328 1107 92-93 0.6477343447778487 1107 94-95 0.4652663996254667 1107 96-97 0.4858669454163369 1107 98-99 0.5214989222282433 1107 100 0.48628482127620387 1108 1 0.1678957830791603 1108 2 0.15100367403813664 1108 3 0.17174040624770726 1108 4 0.24421658057018192 1108 5 0.28868226419977105 1108 6 0.23953040509740475 1108 7 0.30509057536396966 1108 8 0.24111673521182553 1108 9 0.30280475328396506 1108 10-11 0.3308137816861034 1108 12-13 0.384013354664134 1108 14-15 0.4309573640231221 1108 16-17 0.40960192634052106 1108 18-19 0.4388475487148824 1108 20-21 0.3856092889957381 1108 22-23 0.37314676166422345 1108 24-25 0.45017501215252054 1108 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-0.7294338729991843 2316 86-87 -0.774374513092333 2316 88-89 -0.7315765404086925 2316 90-91 -0.6530560069034941 2316 92-93 -0.6259777735779863 2316 94-95 -0.5473518135983113 2316 96-97 -0.5795144809449475 2316 98-99 -0.7893137442266891 2316 100 -0.9570747571661258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24328.0 3 4453.0 4 4226.0 5 4781.0 6 5819.0 7 6716.0 8 8389.0 9 10209.0 10 13263.0 11 18547.0 12 26942.0 13 36745.0 14 37082.0 15 42248.0 16 52482.0 17 68661.0 18 68461.0 19 44093.0 20 40210.0 21 39105.0 22 41610.0 23 48288.0 24 54176.0 25 59611.0 26 66736.0 27 78982.0 28 94361.0 29 114390.0 30 139856.0 31 173050.0 32 218609.0 33 286579.0 34 408389.0 35 642376.0 36 1157636.0 37 2150951.0 38 1901746.0 39 381019.0 40 472.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.499324629596984 17.454664959075007 13.04963884903341 23.996371562294602 2 14.430592242217925 21.90970459581478 36.91316865429141 26.746534507675886 3 19.47238939768604 27.49115373467619 28.97635938898142 24.06009747865635 4 13.455400685525607 18.238874372668686 34.53745516470102 33.768269777104685 5 13.57915923270653 36.568264487054705 33.439105821654906 16.41347045858386 6 31.107229080972832 33.142371703737005 18.741790177698242 17.00860903759192 7 25.45047291393137 32.04499041512203 22.392047786345163 20.112488884601433 8 27.67441208785435 30.98423399493031 21.687031430429567 19.65432248678577 9 27.94273288210769 15.016229469519482 19.60202304833574 37.43901460003709 10-11 24.700184644681393 26.019095258589616 26.037056075962223 23.243664020766772 12-13 27.28197343338191 23.64382144452027 26.18697142493838 22.88723369715944 14-15 26.068794081692058 22.73408168622025 25.12026015515763 26.07686407693006 16-17 23.44579232219965 26.01502154467515 27.562500343012292 22.976685790112906 18-19 24.87417787500891 25.247884988256214 28.52102088642895 21.356916250305925 20-21 24.265917822365505 24.953027394978015 28.059102763825745 22.721952018830738 22-23 23.046353585816988 26.179215861982293 25.447330663069767 25.327099889130956 24-25 23.245890915038025 26.919593939464736 24.880126864712686 24.95438828078455 26-27 24.273034699367717 24.91933574883671 25.30354551250951 25.504084039286067 28-29 23.70999304865631 24.281678422698587 28.019323812583924 23.98900471606118 30-31 25.448878782554154 24.442488312635106 27.073671288476064 23.034961616334677 32-33 21.975562847216256 25.632809060669477 27.67434160896699 24.717286483147276 34-35 21.772961955363606 26.153266099007528 28.036266648856046 24.037505296772824 36-37 23.949089592939323 26.044178711887938 26.387579786052896 23.61915190911984 38-39 24.776732412921557 24.561051021953983 26.117114061609715 24.545102503514745 40-41 23.391377321558693 25.88969072688335 26.07581865271882 24.643113298839133 42-43 25.588004952210287 24.922258490766186 25.7595748353124 23.73016172171113 44-45 25.39751499435523 24.370256923362806 26.59546429508555 23.636763787196415 46-47 23.8496577384315 24.79228206045826 28.40722846810001 22.950831733010226 48-49 24.318859868875244 25.70389534983607 27.588844403040152 22.388400378248534 50-51 22.91086598285478 26.371118686868094 26.34284199609021 24.37517333418691 52-53 22.46771083901328 26.967343396036984 24.58397224827821 25.980973516671526 54-55 23.436113057560522 26.175771314651737 24.393443353635462 25.99467227415228 56-57 24.30760442474055 24.49890828547722 24.895893262878555 26.297594026903674 58-59 24.018209849646105 24.355075607719264 27.14066217539562 24.48605236723901 60-61 24.219975342196037 25.004952977496647 26.198458615896204 24.576613064411116 62-63 21.710409017068667 25.583743784146883 27.096692864123295 25.609154334661156 64-65 21.798525496795445 25.65098949352409 26.892444777629454 25.65804023205101 66-67 23.960504008243877 25.35333898924738 25.49574265622206 25.190414346286683 68-69 24.5046651130685 24.30863675612812 25.536098747402725 25.650599383400653 70-71 23.188667657556785 25.143577876140295 25.83734495936222 25.830409506940704 72-73 24.834778089534982 24.47041923720376 25.79866453724493 24.896138136016326 74-75 25.167061164713395 23.89362415717265 26.199663234182168 24.739651443931788 76-77 23.784013735097435 25.062340781629118 27.324824083179383 23.828821400094068 78-79 23.95806744594701 25.036274558067063 27.103464494029843 23.902193501956084 80-81 22.96985769453522 25.32254559197612 26.541763144189535 25.165833569299128 82-83 23.07428059865103 25.986588506614407 24.898164999585777 26.040965895148787 84-85 23.532977249738952 25.822188687707758 24.789836316789035 25.85499774576426 86-87 23.802615372538614 24.311632480653262 25.580822688460735 26.30492945834739 88-89 24.06954539399559 23.705528412999374 26.707564751976204 25.517361441028836 90-91 24.55974695818268 24.563169915360668 25.980157362570605 24.89692576388605 92-93 23.173919476260387 24.914984820854567 26.51580028954625 25.395295413338797 94-95 22.83520861695344 25.136320482857183 26.77771849818096 25.25075240200842 96-97 24.408479486521205 24.756322942514704 25.491660252316244 25.343537318647847 98-99 24.898318968431163 24.1203344796575 25.248701422794028 25.73264512911731 100 24.627385993927668 24.5505687426601 25.74137661384125 25.080668649570985 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1840.0 1 2383.0 2 2058.0 3 1119.0 4 895.5 5 886.0 6 1244.0 7 1957.5 8 2882.5 9 3125.5 10 2591.5 11 2311.5 12 2523.0 13 2818.0 14 3177.0 15 3539.0 16 3969.5 17 4684.5 18 5438.0 19 6209.0 20 7358.0 21 8742.5 22 10595.5 23 13052.5 24 15938.5 25 19640.5 26 24834.0 27 30983.5 28 37177.0 29 46147.5 30 55378.0 31 62819.5 32 70915.5 33 80443.5 34 90720.5 35 98652.0 36 108053.5 37 118536.0 38 124225.5 39 129851.0 40 137658.5 41 144233.5 42 151500.5 43 168230.0 44 190951.5 45 212984.0 46 250016.0 47 304782.5 48 462691.0 49 646629.5 50 813710.5 51 743872.5 52 474889.5 53 365113.0 54 319234.5 55 279412.5 56 251678.0 57 226766.5 58 213132.0 59 196120.0 60 167321.0 61 137494.5 62 109216.0 63 84244.5 64 62687.0 65 49601.0 66 39237.5 67 29443.0 68 22661.5 69 18715.0 70 16271.0 71 14084.0 72 11801.5 73 11518.0 74 9626.5 75 7526.0 76 5887.0 77 4145.5 78 3052.5 79 2185.5 80 1574.5 81 1223.0 82 960.5 83 789.0 84 563.5 85 335.5 86 239.0 87 166.5 88 117.0 89 107.0 90 85.5 91 66.5 92 54.0 93 44.0 94 41.0 95 35.5 96 26.5 97 16.0 98 13.5 99 11.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11575870461263513 2 0.37490101272249615 3 0.3849178080546462 4 0.380883103532034 5 0.3082001171463631 6 0.13902239109417106 7 0.15335375484645558 8 0.12788614017193206 9 0.210084499073359 10-11 0.21083080280008493 12-13 0.22412433793239117 14-15 0.15176202892929785 16-17 0.13717995376881634 18-19 0.15637978323841478 20-21 0.15655469817436618 22-23 0.16494478460216822 24-25 0.17837825168323557 26-27 0.17081609595227015 28-29 0.17936360582242844 30-31 0.22187959625434825 32-33 0.2191101097684511 34-35 0.13316857123766426 36-37 0.09233759468874295 38-39 0.08632635138987992 40-41 0.08508445534462498 42-43 0.0757265062712252 44-45 0.09763751724807032 46-47 0.1455583792008883 48-49 0.2252671155139403 50-51 0.18124685663283852 52-53 0.11243532082955857 54-55 0.0600366365163848 56-57 0.07587809921571641 58-59 0.1116365426220472 60-61 0.08767319639670568 62-63 0.09666382410460753 64-65 0.10633078956485478 66-67 0.10206869562550572 68-69 0.09280986501581173 70-71 0.12718648042812647 72-73 0.15184948639727355 74-75 0.19626038863533346 76-77 0.19582893179332003 78-79 0.1570327989993 80-81 0.09294979696457284 82-83 0.13264965692767514 84-85 0.16251929749030883 86-87 0.17469337703252613 88-89 0.16218695911200118 90-91 0.1836256997617775 92-93 0.10196957716179993 94-95 0.3436203916765212 96-97 0.34580682837591364 98-99 0.3856816032749673 100 0.3876698030469482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8575597.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.65420454537133 #Duplication Level Percentage of deduplicated Percentage of total 1 78.07086660756516 26.274129138453983 2 11.753280870483678 7.910946369889155 3 3.8018098771980107 3.838406617495047 4 1.7320814819127408 2.3316729792616506 5 0.9669360086500924 1.6270731108697576 6 0.6104081486410025 1.2325680414315432 7 0.43657289639582847 1.0284759487989292 8 0.3192052650783114 0.8594079426323974 9 0.252468894017194 0.7646975820538491 >10 1.662545548604628 11.005181148685296 >50 0.19731341509430597 4.635904378042295 >100 0.16507947895046118 11.202030853011106 >500 0.019145988151121326 4.364302429224043 >1k 0.010080426057787108 6.100705027696623 >5k 8.050340253128784E-4 1.9714355674635045 >10k+ 0.0014000591744571795 14.85306286499099 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 149878 1.7477267180349076 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 123175 1.436343149054229 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 71258 0.8309392337349808 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 60074 0.7005226574896185 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 59871 0.6981554753564095 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 59430 0.6930129762394385 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 58423 0.6812703535392347 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 52956 0.6175196898828151 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 48912 0.5703626231503183 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 43517 0.5074515511864655 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 41243 0.4809344468962336 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 36527 0.42594119103311406 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 26914 0.31384403907972824 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 25849 0.30142507862717893 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 25180 0.2936238724837466 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 23653 0.2758175320038943 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22185 0.2586991902721175 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 20848 0.2431084389809829 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 19223 0.22415932091958143 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 19087 0.22257342550028877 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 18391 0.21445737247214394 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 17102 0.19942634897605382 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 16973 0.1979220805268718 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 16219 0.1891296897463815 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 16135 0.1881501661050537 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 15522 0.18100197572250656 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 15436 0.17999913008971855 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 15045 0.1754396807592521 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14408 0.16801162647918272 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 13481 0.15720188343738636 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 12755 0.14873600053733868 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 12551 0.1463571574083997 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 12339 0.14388502631361993 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 11956 0.13941886494899422 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 11259 0.13129115092511925 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 11122 0.12969359451009652 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10714 0.12493590825221848 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 10491 0.12233550620440768 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10476 0.12216059126845628 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 10350 0.12069130580646456 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 9940 0.11591029755712635 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 9791 0.11417280919334245 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 9547 0.11132752623519973 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 9276 0.10816739639234446 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 9256 0.1079341764777426 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 9092 0.10602177317800732 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 8987 0.10479736862634753 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8587 0.10013297033431025 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.632539402213047E-4 0.0 0.0 0.0 0.0 2 2.6820290179214345E-4 0.0 0.0 1.1660995730093193E-5 0.0 3 2.6820290179214345E-4 0.0 0.0 2.3321991460186387E-5 0.0 4 3.381688761727026E-4 4.664398292037277E-5 0.0 3.498298719027958E-5 0.0 5 0.0013993194876111832 5.8304978650465967E-5 0.0 5.8304978650465967E-5 0.0 6 0.0031601298428552554 5.8304978650465967E-5 0.0 6.996597438055916E-5 0.0 7 0.003498298719027958 5.8304978650465967E-5 0.0 6.996597438055916E-5 0.0 8 0.003918094565311313 6.996597438055916E-5 0.0 8.162697011065235E-5 0.0 9 0.004151314479913177 1.1660995730093193E-4 0.0 1.0494896157083874E-4 0.0 10-11 0.0071773428718723605 1.1660995730093193E-4 0.0 1.749149359513979E-4 0.0 12-13 0.013870754420945854 1.1660995730093193E-4 0.0 2.6820290179214345E-4 0.0 14-15 0.020838199369676538 1.515929444912115E-4 0.0 3.673213654979356E-4 0.0 16-17 0.023228703494345644 1.632539402213047E-4 0.0 6.005412800997995E-4 0.0 18-19 0.024680497462742243 1.924064295465377E-4 0.0 7.871172117812906E-4 0.0 20-21 0.029175811316693168 2.2738941673681727E-4 0.0 0.001212743555929692 2.3321991460186387E-5 22-23 0.030837503208231448 2.623724039270968E-4 0.0 0.002833621962412646 2.3321991460186387E-5 24-25 0.039758164941752744 2.9735539111737646E-4 0.0 0.006075378775378554 2.9152489325232983E-5 26-27 0.06796611361284818 3.148468847125162E-4 0.0 0.012139096555027016 3.498298719027958E-5 28-29 0.07440881375372466 3.148468847125162E-4 0.0 0.03599166332093264 3.498298719027958E-5 30-31 0.07634453904492014 3.964738548231686E-4 0.0 0.09464647184330141 3.498298719027958E-5 32-33 0.07793626496207787 3.964738548231686E-4 0.0 0.19589306726983557 4.664398292037277E-5 34-35 0.07902656806284158 4.4894833560858797E-4 0.0 0.3350612208106328 4.664398292037277E-5 36-37 0.08077571742235555 5.072533142590539E-4 0.0 0.52991645946049 4.664398292037277E-5 38-39 0.08297381511747812 5.130838121241005E-4 0.0 0.8320411978314746 4.664398292037277E-5 40-41 0.08506696385102985 5.364058035842869E-4 0.0 1.2788555712214555 5.247448078541937E-5 42-43 0.09031441192957178 5.364058035842869E-4 0.0 1.6039116576956682 5.8304978650465967E-5 44-45 0.09603996083304754 5.888802843697063E-4 0.0 1.9427976851057718 5.8304978650465967E-5 46-47 0.09815060106019441 6.413547651551257E-4 0.0 2.327820442121989 5.8304978650465967E-5 48-49 0.09956158154353568 6.413547651551257E-4 0.0 2.6717790026746826 6.996597438055916E-5 50-51 0.1018646282002291 6.996597438055916E-4 0.0 3.047391336136714 6.996597438055916E-5 52-53 0.10278001636504142 7.054902416706382E-4 0.0 3.5171603796213837 6.996597438055916E-5 54-55 0.10583519724632581 7.113207395356848E-4 0.0 3.932839894412016 6.996597438055916E-5 56-57 0.11298338762887294 7.404732288609178E-4 0.0 4.37062282660904 6.996597438055916E-5 58-59 0.1155429761916284 7.463037267259644E-4 0.0 4.827955418147564 6.996597438055916E-5 60-61 0.11729212555114238 7.463037267259644E-4 0.0 5.230090686397693 8.162697011065235E-5 62-63 0.118743919519539 7.463037267259644E-4 0.0 5.640837599994496 8.162697011065235E-5 64-65 0.11981090062884252 7.871172117812906E-4 0.0 6.102053303111142 8.162697011065235E-5 66-67 0.12143760953319052 8.046087053764304E-4 0.0 6.588200215098727 8.162697011065235E-5 68-69 0.12314594540764917 8.221001989715702E-4 0.0 7.1888814271472885 8.162697011065235E-5 70-71 0.1254023480814222 8.337611947016633E-4 0.0 7.91997921544121 9.328796584074555E-5 72-73 0.1305565081941234 8.454221904317565E-4 5.830497865046597E-6 8.503163103396766 1.0494896157083874E-4 74-75 0.13600802369794196 8.804051776220361E-4 1.1660995730093193E-5 9.074266199775945 1.1077945943588534E-4 76-77 0.1378038170403763 9.212186626773623E-4 1.1660995730093193E-5 9.691949143598983 1.1660995730093193E-4 78-79 0.13920896702585256 9.328796584074555E-4 1.1660995730093193E-5 10.28046210660319 1.1660995730093193E-4 80-81 0.14134875974232464 9.445406541375487E-4 1.1660995730093193E-5 10.949301838694145 1.1660995730093193E-4 82-83 0.14225831740927192 9.503711520025953E-4 1.1660995730093193E-5 11.734156817303798 1.1660995730093193E-4 84-85 0.14571580264324455 9.562016498676419E-4 1.1660995730093193E-5 12.458916854418415 1.1660995730093193E-4 86-87 0.15211185880120065 9.911846370579216E-4 1.1660995730093193E-5 13.179333170623572 1.2827095303102513E-4 88 0.15359863575678753 0.0010611506114384806 1.1660995730093193E-5 13.764441122874594 1.3993194876111832E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 7650 0.0 29.621939 1 ATCTACA 9730 0.0 23.66947 2 TCTACAC 10850 0.0 23.224539 3 TACACTC 10430 0.0 22.872547 5 AGTACTC 3695 0.0 21.521034 5 CTACACT 12210 0.0 21.132486 4 GTACTAG 1975 0.0 19.08327 1 GTATAAG 2550 0.0 19.02948 1 CACTCTT 12820 0.0 18.524992 7 ACACTCT 14020 0.0 17.339182 6 GTATATA 2290 0.0 17.28119 1 CGTTAAC 685 0.0 17.194094 1 AGTACTT 29965 0.0 16.801607 12-13 ACTCTTT 15540 0.0 16.76342 8 TATTCGC 430 8.0035534E-11 16.403128 9 TATACCG 1245 0.0 16.25137 5 GTCCTAC 1995 0.0 16.058163 1 GTATTAC 1115 0.0 16.056046 1 GAGTACT 28970 0.0 15.958848 12-13 GTCTTAT 1455 0.0 15.865836 1 >>END_MODULE