##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765308_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8492802 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.518744343739556 34.0 31.0 34.0 31.0 34.0 2 32.653615496982034 34.0 31.0 34.0 31.0 34.0 3 32.69523603635172 34.0 31.0 34.0 31.0 34.0 4 36.18616376550401 37.0 35.0 37.0 35.0 37.0 5 36.125057313240085 37.0 35.0 37.0 35.0 37.0 6 36.1932994552328 37.0 37.0 37.0 35.0 37.0 7 36.15023946160525 37.0 36.0 37.0 35.0 37.0 8 36.171567287215694 37.0 37.0 37.0 35.0 37.0 9 37.91456953782745 39.0 38.0 39.0 35.0 39.0 10-11 37.86696045663139 39.0 38.0 39.0 35.0 39.0 12-13 37.83519502750682 39.0 38.0 39.0 35.0 39.0 14-15 39.20683974499818 41.0 39.0 41.0 36.0 41.0 16-17 39.16171771106873 40.0 38.5 41.0 36.0 41.0 18-19 39.18782658538372 40.0 39.0 41.0 36.0 41.0 20-21 39.17693807061556 40.0 39.0 41.0 36.0 41.0 22-23 39.13362551016731 40.0 39.0 41.0 35.5 41.0 24-25 39.084252170249584 40.0 39.0 41.0 35.0 41.0 26-27 38.932273294491026 40.0 38.0 41.0 35.0 41.0 28-29 38.81073248852381 40.0 38.0 41.0 35.0 41.0 30-31 38.636471449587546 40.0 38.0 41.0 35.0 41.0 32-33 38.32631415403303 40.0 38.0 41.0 34.0 41.0 34-35 38.25626601208883 40.0 38.0 41.0 34.0 41.0 36-37 38.17611690464466 40.0 38.0 41.0 34.0 41.0 38-39 38.02119759768331 40.0 38.0 41.0 33.5 41.0 40-41 37.78355370818724 40.0 37.0 41.0 33.0 41.0 42-43 37.52614172566369 40.0 37.0 41.0 33.0 41.0 44-45 37.19914487586076 40.0 36.5 41.0 32.5 41.0 46-47 36.838836405228804 40.0 36.0 41.0 31.0 41.0 48-49 36.52274431924823 39.0 35.5 41.0 31.0 41.0 50-51 36.260381085064736 39.0 35.0 41.0 30.5 41.0 52-53 36.140210616001646 39.0 35.0 41.0 30.0 41.0 54-55 36.13153291457873 39.0 35.0 41.0 30.5 41.0 56-57 35.94978412307269 39.0 35.0 41.0 30.0 41.0 58-59 35.833973287025884 39.0 35.0 41.0 30.0 41.0 60-61 35.492728842612834 38.0 35.0 40.5 30.0 41.0 62-63 35.08622507624692 37.0 35.0 40.0 29.0 41.0 64-65 34.88981757728486 37.0 35.0 39.5 29.0 41.0 66-67 34.50787078281114 36.5 35.0 39.0 29.0 41.0 68-69 34.17622058067526 36.0 34.5 39.0 29.0 41.0 70-71 33.75602580867893 35.0 34.0 37.5 28.0 40.0 72-73 33.34785904581315 35.0 34.0 37.0 28.0 39.0 74-75 33.00281149849013 35.0 34.0 37.0 27.0 39.0 76-77 31.54079325056677 34.0 31.5 35.0 25.0 37.0 78-79 32.26422345652236 35.0 33.0 35.0 26.0 37.0 80-81 32.24404301430788 35.0 34.0 35.0 26.5 37.0 82-83 32.107338838230305 35.0 34.0 35.0 26.5 36.0 84-85 31.935346308556355 35.0 34.0 35.0 26.0 36.0 86-87 31.758877870931173 35.0 33.0 35.0 26.0 36.0 88-89 31.62324324763488 35.0 33.0 35.0 25.0 35.5 90-91 31.49751130427861 35.0 33.0 35.0 25.0 35.0 92-93 31.306431375651993 35.0 33.0 35.0 24.0 35.0 94-95 31.24011009558447 35.0 33.0 35.0 24.0 35.0 96-97 31.154883335323255 35.0 33.0 35.0 23.5 35.0 98-99 31.018606344525637 35.0 33.0 35.0 21.5 35.0 100 30.67648556978015 35.0 33.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4767440581017084 1101 2 0.39040056811475665 1101 3 0.39366422305305804 1101 4 0.28627634738838026 1101 5 0.34316781178803524 1101 6 0.3483048378094793 1101 7 0.31727157723201316 1101 8 0.3092041743492189 1101 9 0.396902975377067 1101 10-11 0.44243128351170213 1101 12-13 0.4162808402266478 1101 14-15 0.5750723926603811 1101 16-17 0.5482351044235401 1101 18-19 0.5633736709062589 1101 20-21 0.5268401728065939 1101 22-23 0.5592534630815251 1101 24-25 0.5807591684787283 1101 26-27 0.6314930909229162 1101 28-29 0.5844268914324005 1101 30-31 0.6709977829077474 1101 32-33 0.7492815159137791 1101 34-35 0.6661446025536932 1101 36-37 0.7478006897279741 1101 38-39 0.7109631296808132 1101 40-41 0.7739689221111448 1101 42-43 0.8570970209781947 1101 44-45 0.915117337335893 1101 46-47 0.9191575931200688 1101 48-49 0.953416666186726 1101 50-51 1.0312906333385783 1101 52-53 1.0191153978878447 1101 54-55 1.0773868517905214 1101 56-57 1.0888306692612417 1101 58-59 1.1647413933305089 1101 60-61 1.2084785883962468 1101 62-63 1.1805895421667358 1101 64-65 1.0886429310190593 1101 66-67 1.1143063684866732 1101 68-69 1.1062445934740452 1101 70-71 1.0994615811917825 1101 72-73 1.1241326740321824 1101 74-75 1.1642126550101821 1101 76-77 1.219845943799708 1101 78-79 1.2157472425859908 1101 80-81 1.138003832739237 1101 82-83 1.1715895003531003 1101 84-85 1.204084889809824 1101 86-87 1.2103457166011395 1101 88-89 1.291077952745706 1101 90-91 1.2592153952438707 1101 92-93 1.3817027036423077 1101 94-95 1.332945454079205 1101 96-97 1.3967535054620122 1101 98-99 1.4381435775215827 1101 100 1.4540363218187728 1102 1 0.362278770059028 1102 2 0.31777419389173645 1102 3 0.32601999420918304 1102 4 0.2337511640740928 1102 5 0.23648705267132897 1102 6 0.2547980464875579 1102 7 0.3324230778264976 1102 8 0.2731835983535973 1102 9 0.3460273060909458 1102 10-11 0.35560579495810885 1102 12-13 0.35137403793327593 1102 14-15 0.4305789755668954 1102 16-17 0.4761386265057652 1102 18-19 0.4799134093681161 1102 20-21 0.45726079021346777 1102 22-23 0.4675439324911963 1102 24-25 0.4631917854955745 1102 26-27 0.48838426313823646 1102 28-29 0.5563450234960214 1102 30-31 0.6217507954817805 1102 32-33 0.5792386398438722 1102 34-35 0.6199797449307809 1102 36-37 0.6923142219317242 1102 38-39 0.6804973610688378 1102 40-41 0.7287935104526255 1102 42-43 0.752407151619316 1102 44-45 0.7620412779188044 1102 46-47 0.8150377026574418 1102 48-49 0.8224299926113119 1102 50-51 0.7808594672154427 1102 52-53 0.8036225192203688 1102 54-55 0.8818687298781782 1102 56-57 0.9281567033997078 1102 58-59 0.9714443109377342 1102 60-61 1.027530763650688 1102 62-63 0.9189783911969513 1102 64-65 0.969626247779992 1102 66-67 0.9644357095361471 1102 68-69 0.9533752974253389 1102 70-71 0.9228365016095026 1102 72-73 0.8798647364517294 1102 74-75 0.8859299933283609 1102 76-77 0.908365265705843 1102 78-79 0.921677453135942 1102 80-81 0.8881712575226288 1102 82-83 0.9411304313101212 1102 84-85 0.9593314736681968 1102 86-87 0.945966007995132 1102 88-89 0.9735383475488 1102 90-91 0.9439688751239714 1102 92-93 0.9729979224816567 1102 94-95 1.0326046782409968 1102 96-97 1.024696678963256 1102 98-99 0.9967599321044851 1102 100 1.0629597259206243 1103 1 0.3540200417460966 1103 2 0.30205102020114794 1103 3 0.2948429990775452 1103 4 0.24675092672139698 1103 5 0.27562504019639533 1103 6 0.25291387660790576 1103 7 0.23456889163647787 1103 8 0.22561507561785987 1103 9 0.2595854662372119 1103 10-11 0.3013618740588413 1103 12-13 0.28371146534276903 1103 14-15 0.3808196118192555 1103 16-17 0.4206940011229676 1103 18-19 0.36793187848422093 1103 20-21 0.39274323485089013 1103 22-23 0.41081144511105805 1103 24-25 0.468366841257577 1103 26-27 0.4729753163212038 1103 28-29 0.4691445997558361 1103 30-31 0.5053464074416425 1103 32-33 0.48792326324357305 1103 34-35 0.4810531133316758 1103 36-37 0.5457434060964275 1103 38-39 0.5440194062347459 1103 40-41 0.5030420371144189 1103 42-43 0.5070479443622631 1103 44-45 0.48960253418422184 1103 46-47 0.5214501986454749 1103 48-49 0.4302317250267933 1103 50-51 0.5204501742764904 1103 52-53 0.5726747232687686 1103 54-55 0.67199057315635 1103 56-57 0.6756319257934962 1103 58-59 0.6097977721528096 1103 60-61 0.6235134982697303 1103 62-63 0.6004274912506844 1103 64-65 0.6118321442596084 1103 66-67 0.6103674120722715 1103 68-69 0.5361267272056836 1103 70-71 0.4359709194805035 1103 72-73 0.5435171395787677 1103 74-75 0.5662972429039215 1103 76-77 0.5157503872953804 1103 78-79 0.5901568672000082 1103 80-81 0.6085987966885398 1103 82-83 0.6105743153733201 1103 84-85 0.6013637030537318 1103 86-87 0.5368833757328701 1103 88-89 0.5493844359070401 1103 90-91 0.6279703108611301 1103 92-93 0.628889178915113 1103 94-95 0.6811920723132623 1103 96-97 0.7134473318085632 1103 98-99 0.7319844970965299 1103 100 0.8466419038936763 1104 1 0.3144437672292568 1104 2 0.23038356768154955 1104 3 0.279681719137578 1104 4 0.20769271883536078 1104 5 0.229884986935609 1104 6 0.22403733761198907 1104 7 0.21129504819634803 1104 8 0.18779793582414328 1104 9 0.26014489701482546 1104 10-11 0.24885219111259715 1104 12-13 0.2778181071782555 1104 14-15 0.36164481523278624 1104 16-17 0.3715856436542708 1104 18-19 0.30816258122065676 1104 20-21 0.33420774944200105 1104 22-23 0.3407685366788158 1104 24-25 0.32677085585367394 1104 26-27 0.36941116733671464 1104 28-29 0.4004781223739968 1104 30-31 0.4395393636608844 1104 32-33 0.39814302917746147 1104 34-35 0.4389710480138902 1104 36-37 0.4635112756772699 1104 38-39 0.4813958032311163 1104 40-41 0.4487100327023441 1104 42-43 0.4639108149054536 1104 44-45 0.47053661708450534 1104 46-47 0.456607156813412 1104 48-49 0.458504281023572 1104 50-51 0.48938657112630324 1104 52-53 0.49348972771699096 1104 54-55 0.4797504404002524 1104 56-57 0.4269231499344386 1104 58-59 0.4816652597466131 1104 60-61 0.5370244349645148 1104 62-63 0.5546345339106793 1104 64-65 0.5124868239329956 1104 66-67 0.5031063806332909 1104 68-69 0.5038824587533455 1104 70-71 0.5162805420634626 1104 72-73 0.507942554400266 1104 74-75 0.520677258823099 1104 76-77 0.41394443906282063 1104 78-79 0.5338441074796876 1104 80-81 0.4707408064321541 1104 82-83 0.5524114575249541 1104 84-85 0.5230306688306534 1104 86-87 0.5618404866984932 1104 88-89 0.5006999756285104 1104 90-91 0.5291064347183578 1104 92-93 0.540611345262711 1104 94-95 0.5436411699857402 1104 96-97 0.6202435455468631 1104 98-99 0.5622808016096421 1104 100 0.611752875116661 1105 1 0.2082150003433938 1105 2 0.1466257303375258 1105 3 0.17869061333451697 1105 4 0.1331242828362207 1105 5 0.13419082467665078 1105 6 0.08854054939428835 1105 7 0.1396823753226073 1105 8 0.1503399550499438 1105 9 0.15492526701844156 1105 10-11 0.18139095933907612 1105 12-13 0.2379658778517566 1105 14-15 0.26004120081449855 1105 16-17 0.27730287283016963 1105 18-19 0.2270511812907614 1105 20-21 0.2267018349039489 1105 22-23 0.273598655157123 1105 24-25 0.29276007073694643 1105 26-27 0.24090436936283055 1105 28-29 0.2809318366387288 1105 30-31 0.2504789882884708 1105 32-33 0.2864303008595215 1105 34-35 0.29337681166775553 1105 36-37 0.28982413783371186 1105 38-39 0.3500382976463712 1105 40-41 0.34618994798722724 1105 42-43 0.3222624859357879 1105 44-45 0.36290615375074964 1105 46-47 0.3241247387120083 1105 48-49 0.2554199957471539 1105 50-51 0.26743587299585414 1105 52-53 0.3149643543070155 1105 54-55 0.3850739259578546 1105 56-57 0.4290022796234112 1105 58-59 0.3613884567432635 1105 60-61 0.3522941030137261 1105 62-63 0.3024566226352263 1105 64-65 0.27644212591078343 1105 66-67 0.2939771676747185 1105 68-69 0.30385886496948444 1105 70-71 0.2972262451300125 1105 72-73 0.22650910607538322 1105 74-75 0.2770210185851383 1105 76-77 0.2426812545758814 1105 78-79 0.2826469315499267 1105 80-81 0.325475587746233 1105 82-83 0.3698147538046328 1105 84-85 0.36064001854893846 1105 86-87 0.3380215327923537 1105 88-89 0.3613297778754152 1105 90-91 0.42023374157891524 1105 92-93 0.41025177965216386 1105 94-95 0.4177821444733816 1105 96-97 0.4321039555351476 1105 98-99 0.4442778147589834 1105 100 0.5439009512617226 1106 1 0.1972017864376241 1106 2 0.09243460209645349 1106 3 0.14458588996103572 1106 4 0.1368137301861836 1106 5 0.14317629833677614 1106 6 0.12095845501549718 1106 7 0.1327532405566174 1106 8 0.12658944995494892 1106 9 0.18517474477903306 1106 10-11 0.18047696279101189 1106 12-13 0.18582270104590037 1106 14-15 0.28450744805402906 1106 16-17 0.23438599690810236 1106 18-19 0.16147754708683948 1106 20-21 0.16002230500365755 1106 22-23 0.1948833803455372 1106 24-25 0.22753425373932856 1106 26-27 0.2705485534027545 1106 28-29 0.26534814487392566 1106 30-31 0.24585530364155517 1106 32-33 0.23404113872750543 1106 34-35 0.2193370242229591 1106 36-37 0.2713986789177696 1106 38-39 0.2579372443588781 1106 40-41 0.22732658242733095 1106 42-43 0.17889650038497962 1106 44-45 0.27988610247714263 1106 46-47 0.23198811816478582 1106 48-49 0.27694998944805604 1106 50-51 0.23105593869441066 1106 52-53 0.29370304749510723 1106 54-55 0.3167474686165477 1106 56-57 0.29010921876400886 1106 58-59 0.28565919859664035 1106 60-61 0.23200477336899894 1106 62-63 0.2281723869492609 1106 64-65 0.2752672313562883 1106 66-67 0.2468883844637091 1106 68-69 0.2437132099096786 1106 70-71 0.24272690179045497 1106 72-73 0.2601911975869271 1106 74-75 0.2913686353792073 1106 76-77 0.17494698878373427 1106 78-79 0.25924649755989293 1106 80-81 0.2973259675983613 1106 82-83 0.2752271699632942 1106 84-85 0.2662264726328054 1106 86-87 0.26118883237346324 1106 88-89 0.2858984334019574 1106 90-91 0.3170658685468659 1106 92-93 0.3090299448870262 1106 94-95 0.33496786306209003 1106 96-97 0.4448717593392537 1106 98-99 0.42023157599115635 1106 100 0.4596676300378597 1107 1 0.15694936462774223 1107 2 0.04701573123783476 1107 3 0.11502775741743676 1107 4 0.08663342246910588 1107 5 0.13926074953374012 1107 6 0.13138483476077312 1107 7 0.07547209385439402 1107 8 0.08348693403383578 1107 9 0.06795803352073904 1107 10-11 0.1463941610982502 1107 12-13 0.18940459962612266 1107 14-15 0.20679687937756341 1107 16-17 0.23333181285391902 1107 18-19 0.1629143144813412 1107 20-21 0.2373109695865523 1107 22-23 0.18229787246306017 1107 24-25 0.18039921047694207 1107 26-27 0.24186427471119032 1107 28-29 0.2101388280533243 1107 30-31 0.2948553704221908 1107 32-33 0.3217853574244174 1107 34-35 0.32765414769990997 1107 36-37 0.28748275011717084 1107 38-39 0.31012307912919823 1107 40-41 0.35616289949099667 1107 42-43 0.31531320660661777 1107 44-45 0.32055247428968414 1107 46-47 0.3115617710228591 1107 48-49 0.23472914093824215 1107 50-51 0.2628259549533567 1107 52-53 0.2794521553440319 1107 54-55 0.23039490665726703 1107 56-57 0.3215292550234139 1107 58-59 0.2751284038418689 1107 60-61 0.23184650663455386 1107 62-63 0.3018551782539234 1107 64-65 0.21569229909816556 1107 66-67 0.23923837593063269 1107 68-69 0.19389016173116147 1107 70-71 0.24748991260329234 1107 72-73 0.22861385020821956 1107 74-75 0.18714443974297978 1107 76-77 0.1340645636258273 1107 78-79 0.17872765698865578 1107 80-81 0.22084094725949655 1107 82-83 0.18580944426891932 1107 84-85 0.2093408381819124 1107 86-87 0.21928738417804539 1107 88-89 0.2198992095863197 1107 90-91 0.30345703735864404 1107 92-93 0.2999071085402818 1107 94-95 0.3331210243382223 1107 96-97 0.3852813686005838 1107 98-99 0.3382145556981655 1107 100 0.340077845271626 1108 1 0.09186781379092679 1108 2 0.04446919024825746 1108 3 0.06590410494150234 1108 4 0.06644336549081231 1108 5 0.13010379201460154 1108 6 0.0701652197710132 1108 7 0.039884510444409216 1108 8 0.06692284144084226 1108 9 0.11291303548333076 1108 10-11 0.13351377971091694 1108 12-13 0.09329724981448351 1108 14-15 0.09442143297186334 1108 16-17 0.10302786789128504 1108 18-19 0.15752751422005673 1108 20-21 0.13842016944017388 1108 22-23 0.09622260576654185 1108 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-0.7903486246842206 2316 54-55 -0.8525265267665532 2316 56-57 -0.774396100740411 2316 58-59 -0.8165624280657156 2316 60-61 -0.7456784015440121 2316 62-63 -0.6931086600538308 2316 64-65 -0.7526158978536515 2316 66-67 -0.6677450451260611 2316 68-69 -0.6279213824450522 2316 70-71 -0.6784218904422445 2316 72-73 -0.6009191167823147 2316 74-75 -0.6093433390450969 2316 76-77 -0.6382740698123079 2316 78-79 -0.7035824709022442 2316 80-81 -0.6966741360919961 2316 82-83 -0.661458975108296 2316 84-85 -0.6795912449978552 2316 86-87 -0.7190144439803419 2316 88-89 -0.655806594039035 2316 90-91 -0.6599810031431055 2316 92-93 -0.7290045645785135 2316 94-95 -0.7333828150101844 2316 96-97 -0.707425762713644 2316 98-99 -0.7495231701858067 2316 100 -0.8208205897510794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 40.0 4 48.0 5 112.0 6 295.0 7 732.0 8 1692.0 9 3152.0 10 5752.0 11 10477.0 12 17147.0 13 24287.0 14 28166.0 15 36998.0 16 49663.0 17 66732.0 18 67535.0 19 43271.0 20 29062.0 21 25474.0 22 27124.0 23 30631.0 24 34464.0 25 38879.0 26 47545.0 27 60013.0 28 76061.0 29 97080.0 30 121928.0 31 154378.0 32 197430.0 33 257983.0 34 366677.0 35 597433.0 36 1174038.0 37 2387693.0 38 2100691.0 39 311826.0 40 279.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.739780816743405 17.823893692564596 13.113940487485756 24.322385003206247 2 14.75592922072291 22.504741114416383 35.36043073448628 27.378898930374422 3 20.3444772598039 27.318869187633766 28.969564473858394 23.36708907870394 4 14.311709590208324 19.058163185881433 32.93806440809812 33.69206281581212 5 13.638149105560215 37.233954117851795 33.137956118604905 15.989940657983079 6 32.14280752100426 32.214303359480176 18.095453067197376 17.547436052318186 7 25.26232214056091 32.19491046653389 22.324175225090613 20.21859216781458 8 28.52026928215211 30.87087159220243 21.36174845475027 19.24711067089519 9 28.666487220589858 14.353849294967668 19.35056298262929 37.629100501813184 10-11 25.241653351949893 25.925662521839836 25.204869232986947 23.62781489322332 12-13 28.036651330714285 23.73236422878069 25.528287911561677 22.70269652894335 14-15 26.973193313040777 22.90389624396943 24.324392904128207 25.79851753886159 16-17 23.680478402470904 25.970821871862587 27.869278858576752 22.47942086708976 18-19 25.52780578188447 25.014058964285287 28.478039403249948 20.98009585058029 20-21 24.40763676308803 25.119360484264387 27.864101139928394 22.608901612719194 22-23 23.10415341211702 26.06199779166397 24.836250011333707 25.997598784885295 24-25 23.563902443102474 27.172952825096097 24.106683273779574 25.156461458021855 26-27 25.021182728646302 24.66251874536076 24.953177809637562 25.363120716355375 28-29 24.09521738203775 23.95686425001934 27.85974257686197 24.088175791080943 30-31 26.02641002000563 24.59667875953329 26.500439794126894 22.876471426334188 32-33 22.136714135282553 25.70500850200861 27.455194161479174 24.703083201229667 34-35 21.623058472777114 26.671941803932096 27.937898211045525 23.767101512245265 36-37 24.8796533625907 25.861861377417995 25.870722191688557 23.387763068302746 38-39 25.62188520155642 24.404464756144108 25.55192284040003 24.421727201899436 40-41 23.735278419078742 25.96389919412146 25.93694080776722 24.363881579032583 42-43 26.350886358876863 24.789193957102444 25.620984620518506 23.238935063502186 44-45 26.35023764615882 24.148018312274548 25.841746515434565 23.65999752613207 46-47 24.171252014982404 25.019140125026052 28.476155207515223 22.333452652476325 48-49 25.503962179728713 25.648390401281084 27.064560568952523 21.783086850037677 50-51 23.094153374868107 26.58254326715488 26.21715053841307 24.106152819563945 52-53 22.552360738857445 27.121751707715546 23.736465951727663 26.58942160169935 54-55 23.76217271633409 26.828103006712823 23.291065004667857 26.11865927228523 56-57 24.99820537410221 24.409503302317763 24.04682692769174 26.545464395888292 58-59 24.180839463138106 24.29268323707871 26.473754608800316 25.052722690982872 60-61 25.085670990888538 25.209548841062528 25.301043904675062 24.403736263373872 62-63 21.89636894771875 25.910742310718877 26.309283424972453 25.883605316589925 64-65 21.399676492111396 26.349236498185192 26.475716978347062 25.77537003135635 66-67 24.488616045592572 25.68613813109878 24.782965827354435 25.042279995954214 68-69 25.111225170093498 24.56190517907064 24.509760285384523 25.81710936545134 70-71 23.253686567819695 25.7594941403833 24.942889799124927 26.04392949267208 72-73 25.24917759149052 24.87170804044585 24.84482625038003 25.0342881176836 74-75 25.616453301120817 24.15333069448794 25.001676508759264 25.22853949563198 76-77 23.509007074653294 25.34931417086625 27.11847792336795 24.023200831112508 78-79 24.19599451151972 25.592700410717583 26.337145903121232 23.87415917464146 80-81 22.318229013227906 26.08305833575303 26.024491092574632 25.57422155844443 82-83 22.045439024103054 26.68485287364442 23.825650264344674 27.44405783790785 84-85 22.800195977723252 26.56583186561985 23.373546210072956 27.260425946583943 86-87 23.569762444437902 24.583538900827175 24.215577992994024 27.631120661740898 88-89 23.31233081979038 24.055702287810384 25.85430376084758 26.77766313155166 90-91 24.242668479350673 24.945615358611295 24.87769269471939 25.93402346731864 92-93 21.84827256188539 25.59619924581426 25.58433027850685 26.9711979137935 94-95 20.940278851335357 26.137919190585894 26.224776532068304 26.697025426010445 96-97 23.371187597292078 25.47143956983279 24.729522057561645 26.427850775313484 98-99 23.789068672506435 24.747109375680722 24.436428636862136 27.02739331495071 100 22.73587954881437 25.894226064983776 25.349996202636728 26.019898183565132 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 845.0 1 560.0 2 313.5 3 347.5 4 297.0 5 350.5 6 593.5 7 1164.5 8 1890.0 9 2104.0 10 1733.0 11 1532.5 12 1698.5 13 1929.0 14 2269.5 15 2691.0 16 3156.5 17 3730.5 18 4448.0 19 5193.5 20 6118.0 21 7321.5 22 8926.5 23 10664.5 24 12718.0 25 15626.5 26 19879.0 27 25187.0 28 30628.0 29 38349.5 30 47240.0 31 54789.5 32 63337.0 33 72633.0 34 82159.5 35 89345.5 36 95523.0 37 104740.5 38 111232.5 39 117675.0 40 121875.0 41 124954.0 42 132825.0 43 150125.0 44 173616.5 45 194843.5 46 224683.0 47 270457.5 48 385251.0 49 597263.5 50 996403.5 51 974146.0 52 525387.0 53 346856.5 54 299450.0 55 267448.5 56 245556.0 57 220436.0 58 205368.0 59 189675.0 60 161427.5 61 133278.0 62 109561.5 63 86927.5 64 64973.0 65 51256.0 66 39666.0 67 28515.5 68 21319.5 69 16880.0 70 14512.5 71 12606.0 72 10765.0 73 11344.5 74 9337.0 75 6926.0 76 5302.5 77 3490.0 78 2489.5 79 1632.5 80 1087.0 81 783.0 82 578.0 83 403.0 84 250.0 85 136.0 86 87.0 87 51.5 88 24.0 89 15.5 90 13.0 91 8.5 92 4.5 93 3.5 94 2.0 95 0.5 96 1.0 97 1.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06294742300597611 3 0.02588073994895913 4 0.00717077826611288 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.653540021302746E-5 12-13 1.589581389039801E-4 14-15 3.8856433954306244E-4 16-17 4.297757088885388E-4 18-19 0.0 20-21 0.002955443915918445 22-23 0.005121984475794914 24-25 6.53494571049696E-4 26-27 4.0033901649891285E-4 28-29 0.004745194813207702 30-31 0.0022607379755232725 32-33 0.0012127917264525888 34-35 0.0015542573581722497 36-37 0.004174122980848958 38-39 0.004456715227789368 40-41 9.772981873355812E-4 42-43 0.0025963162687650085 44-45 3.3557829324173575E-4 46-47 0.0020252444364062647 48-49 2.3549353911700755E-5 50-51 0.0018721736359802103 52-53 0.00717077826611288 54-55 0.014359218547659536 56-57 0.025604035040496645 58-59 0.028112041232092776 60-61 0.025668795763753825 62-63 0.008295259915396591 64-65 0.0068587493267828455 66-67 1.7662015433775566E-4 68-69 0.0014129612347020452 70-71 1.2952144651435415E-4 72-73 7.535793251744242E-4 74-75 0.005316266645566446 76-77 0.0010479462490706837 78-79 0.0012363410803642896 80-81 0.0 82-83 0.0012010170494967385 84-85 0.0 86-87 6.122832017042197E-4 88-89 1.059720926026534E-4 90-91 6.476072325717708E-4 92-93 7.830160175640501E-4 94-95 0.001654342112296978 96-97 1.3540878499227935E-4 98-99 0.0 100 7.889033560419753E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8492802.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.67928531244738 #Duplication Level Percentage of deduplicated Percentage of total 1 76.1203644998064 23.353183805770513 2 12.950200074259707 7.946057658629815 3 4.022369812487507 3.702102933284391 4 1.8785930845808703 2.3053557291138844 5 1.0292652512775704 1.578856115306621 6 0.6977530329623916 1.2843938625527227 7 0.48359187261860653 1.0385377124392856 8 0.3541925022078198 0.869309826461068 9 0.27235921977352573 0.75202075898168 >10 1.8025640078091372 10.633023565221205 >50 0.19563080754363685 4.199423573705592 >100 0.15758966186147502 10.023774224029056 >500 0.021889108255536718 4.613158977295101 >1k 0.01123508159423632 6.142975012150723 >5k 6.973498920352745E-4 1.5604451354894167 >10k+ 0.00170463306941956 19.997381109569027 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 212903 2.506864047931413 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 199813 2.3527335265793314 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 97597 1.1491731468601294 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 72688 0.8558777185668522 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 71693 0.8441619149957811 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 70555 0.8307623326200233 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 68016 0.8008664278291192 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 66702 0.7853945023091318 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 53554 0.6305810496936112 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 49716 0.5853898395370574 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 44647 0.5257040020478518 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 38523 0.4535958803702241 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 34463 0.4057906919294716 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 32794 0.3861387560901573 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 31687 0.3731041887000309 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 29203 0.34385589114169857 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 28621 0.3370030291533937 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 28302 0.3332469072044774 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 27908 0.32860768448387234 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 25647 0.3019851398866946 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 23681 0.2788361249914928 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 21418 0.2521900310404034 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21207 0.24970557420271894 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 21038 0.24771565379718025 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 20798 0.24488973132777617 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 20402 0.2402269592532594 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 19269 0.22688625026228093 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 19253 0.22669785543098733 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 18934 0.22294173348207108 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 17509 0.20616281881998427 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 16820 0.19805006639740333 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16408 0.193198899491593 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 16239 0.1912089790860543 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 15426 0.18163616672094793 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 14185 0.16702379261873762 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 14149 0.166599904248327 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 14146 0.16656458021745946 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 13900 0.16366800968632025 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 13234 0.1558260748337239 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 12528 0.14751315290289355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12370 0.14565275394386917 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 12271 0.14448706092524 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 12116 0.14266198599708318 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 10146 0.11946587239405793 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 9925 0.116863668786815 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT 9338 0.10995193341373083 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 9213 0.10848009879424952 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 9203 0.10836235202469104 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 8660 0.10196870243766426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0028494718233157915 0.0 0.0 0.0 0.0 2 0.0049924630292805606 0.0 0.0 0.0 0.0 3 0.005263280599265119 0.0 0.0 1.1774676955850378E-5 0.0 4 0.006099282663130496 0.0 0.0 3.532403086755113E-5 0.0 5 0.02686981281325056 0.0 0.0 2.0016950824945642E-4 0.0 6 0.05767236772975515 0.0 0.0 3.885643395430625E-4 0.0 7 0.06516106227367599 0.0 0.0 4.238883704106136E-4 0.0 8 0.07415691546794569 0.0 0.0 4.945364321457159E-4 0.0 9 0.0806800865014868 0.0 0.0 6.005085247483692E-4 0.0 10-11 0.1404954454372067 0.0 0.0 0.001148031003195412 0.0 12-13 0.24317651583069994 0.0 0.0 0.0029142325465729684 1.7662015433775566E-5 14-15 0.3628307830560515 0.0 0.0 0.005033674398626037 2.3549353911700755E-5 16-17 0.4153399549406662 0.0 0.0 0.006028634601395393 2.3549353911700755E-5 18-19 0.4394839300386374 0.0 0.0 0.006487847002673558 2.3549353911700755E-5 20-21 0.5295131100430694 0.0 0.0 0.008265823223006966 2.3549353911700755E-5 22-23 0.5560944432709016 0.0 0.0 0.010302842336369081 2.3549353911700755E-5 24-25 0.6721221099938512 0.0 0.0 0.019716696562571457 2.3549353911700755E-5 26-27 0.9149748222082652 0.0 0.0 0.04928291039871176 2.3549353911700755E-5 28-29 0.9745311382509565 0.0 0.0 0.07865484206508053 2.3549353911700755E-5 30-31 0.992670028101444 0.0 0.0 0.14665360148511644 2.3549353911700755E-5 32-33 1.0086894760998786 0.0 0.0 0.2610445881112029 2.3549353911700755E-5 34-35 1.0210882109343888 0.0 0.0 0.41335003453512753 2.3549353911700755E-5 36-37 1.0396922005246325 0.0 0.0 0.6300806259229874 2.3549353911700755E-5 38-39 1.0595148691798066 0.0 0.0 0.942545228300389 2.3549353911700755E-5 40-41 1.0771238985672809 0.0 0.0 1.3809223387051763 2.3549353911700755E-5 42-43 1.1128011697435074 0.0 0.0 1.7020236666296942 2.3549353911700755E-5 44-45 1.154848541152849 0.0 0.0 2.0352941231880832 2.3549353911700755E-5 46-47 1.1728402475413886 0.0 0.0 2.4239644348237483 2.3549353911700755E-5 48-49 1.1844147549889894 0.0 0.0 2.7707875445583214 2.3549353911700755E-5 50-51 1.204761396768699 0.0 0.0 3.1386049033051755 2.3549353911700755E-5 52-53 1.211437638602666 0.0 0.0 3.5781594814055477 2.3549353911700755E-5 54-55 1.2324083382610356 0.0 0.0 3.972110735655912 3.532403086755113E-5 56-57 1.2742908641929955 5.887338477925189E-6 0.0 4.393967974291641 4.709870782340151E-5 58-59 1.28761391116854 1.1774676955850378E-5 0.0 4.837684900695907 5.887338477925189E-5 60-61 1.2971749488566906 1.1774676955850378E-5 1.1774676955850378E-5 5.227650426796716 5.887338477925189E-5 62-63 1.304693080093001 2.3549353911700755E-5 1.1774676955850378E-5 5.628819558021016 5.887338477925189E-5 64-65 1.3106275172787498 2.3549353911700755E-5 1.1774676955850378E-5 6.075450716971854 5.887338477925189E-5 66-67 1.318863903809367 2.3549353911700755E-5 1.1774676955850378E-5 6.537471378703989 5.887338477925189E-5 68-69 1.3284602655283853 2.3549353911700755E-5 1.1774676955850378E-5 7.088249555329325 5.887338477925189E-5 70-71 1.340034772975986 3.532403086755113E-5 1.1774676955850378E-5 7.786487898811252 5.887338477925189E-5 72-73 1.3693772679499652 3.532403086755113E-5 1.1774676955850378E-5 8.338031429438718 6.476072325717707E-5 74-75 1.3980839303683283 3.532403086755113E-5 2.3549353911700755E-5 8.862817006684013 7.064806173510227E-5 76-77 1.4072681783938916 3.532403086755113E-5 2.3549353911700755E-5 9.437556650914503 7.064806173510227E-5 78-79 1.4140974910282849 3.532403086755113E-5 2.3549353911700755E-5 9.985179214115671 7.064806173510227E-5 80-81 1.4272144811571024 3.532403086755113E-5 2.3549353911700755E-5 10.595631453553256 7.064806173510227E-5 82-83 1.4321716201555152 3.532403086755113E-5 2.3549353911700755E-5 11.319691663599365 7.064806173510227E-5 84-85 1.4496393534195193 3.532403086755113E-5 2.3549353911700755E-5 11.986091280592671 7.064806173510227E-5 86-87 1.4794233987793428 3.532403086755113E-5 2.3549353911700755E-5 12.652208305339038 7.064806173510227E-5 88 1.486729585830448 3.532403086755113E-5 2.3549353911700755E-5 13.196233704730194 7.064806173510227E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 2320 0.0 28.366137 3 GATCTAC 3515 0.0 23.552479 1 CGATCTC 2390 0.0 23.201107 5 TCTACAC 5045 0.0 20.68483 3 ATCTACA 4295 0.0 19.713266 2 TACACTC 4700 0.0 19.296745 5 AGTACTT 27525 0.0 18.830975 12-13 TCTCGCG 1165 0.0 18.55492 8 CCGATCT 3630 0.0 18.254408 4 AGTACTC 2965 0.0 17.59231 5 CTACACT 6735 0.0 17.514244 4 GAGTACT 26935 0.0 17.149881 12-13 TACTTTT 31960 0.0 16.247929 14-15 TAGTACT 2350 0.0 16.198412 4 GTATAAG 2250 0.0 16.097464 1 GTACTTT 29575 0.0 15.619649 14-15 CATGGGT 8975 0.0 15.604042 4 GCATAGA 1695 0.0 15.263082 1 CGTTAAC 655 0.0 15.080899 1 ATGGGTA 5795 0.0 14.8396015 5 >>END_MODULE