FastQCFastQC Report
Fri 27 May 2016
ERR765307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15168600
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA390420.25738697045211817No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT389610.25685297258810963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312650.20611658294107565No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA246540.1625331276452672No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC198830.13107999419854172No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC190240.12541697981356223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183090.12070329496459793No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC178660.11778278812810675No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA173610.11445354218583126No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG170500.11240325409068734No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA163470.10776868003639097No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC163060.10749838482127554No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA161340.10636446343103517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157610.10390543623010694No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC154020.10153870495629128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG39750.030.6237471
CTATTGA97650.027.8181579
AGGGCTA107750.025.603165
GTACTAG75050.025.4255891
GTATAAG69600.024.445281
GTATCAA867200.024.3019981
GTGATCG57800.023.9865048
TTTAGGG116900.023.6398982
ATGGGAG161050.023.5788925
ATTTAGG118750.023.5097751
CGTTAAC20900.023.387441
TAGTACT83750.023.2882064
TAGGCAT103000.023.224875
GGGCTAT125400.023.1238616
ACGTTAA15150.022.9557698
GGCTATT127750.022.4409737
TATTCGC12300.022.1613229
TAAGGTG67650.022.161324
CTGTGCG89750.021.7836979
TATTCTC61100.021.7679735
Produced by FastQC (version 0.11.5)