##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765307_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15168600
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14856974275806	33.0	33.0	33.0	33.0	33.0
2	32.04023265166199	33.0	33.0	33.0	33.0	33.0
3	32.04738782748573	33.0	33.0	33.0	33.0	33.0
4	35.8516118165157	37.0	37.0	37.0	33.0	37.0
5	35.855942736969794	37.0	37.0	37.0	33.0	37.0
6	35.867149769919436	37.0	37.0	37.0	33.0	37.0
7	35.79222907849109	37.0	37.0	37.0	33.0	37.0
8	35.76383482984586	37.0	37.0	37.0	33.0	37.0
9	35.73962574001556	37.0	37.0	37.0	33.0	37.0
10-11	35.73766764895903	37.0	37.0	37.0	33.0	37.0
12-13	35.71497366269794	37.0	37.0	37.0	33.0	37.0
14-15	37.561008135226714	40.0	37.0	40.0	33.0	40.0
16-17	37.62391021584062	40.0	37.0	40.0	33.0	40.0
18-19	37.68643239982596	40.0	37.0	40.0	33.0	40.0
20-21	37.72838333794813	40.0	37.0	40.0	33.0	40.0
22-23	37.723773024537536	40.0	37.0	40.0	33.0	40.0
24-25	37.739130374589614	40.0	37.0	40.0	33.0	40.0
26-27	37.73301412127685	40.0	37.0	40.0	33.0	40.0
28-29	37.68729793784529	40.0	37.0	40.0	33.0	40.0
30-31	37.61314096225097	40.0	37.0	40.0	33.0	40.0
32-33	37.52214406734966	40.0	37.0	40.0	33.0	40.0
34-35	37.39263481797924	40.0	37.0	40.0	33.0	40.0
36-37	37.27834131033846	40.0	37.0	40.0	33.0	40.0
38-39	37.114729704784885	40.0	37.0	40.0	33.0	40.0
40-41	36.995021063249084	40.0	37.0	40.0	33.0	40.0
42-43	36.80921795683187	38.5	37.0	40.0	33.0	40.0
44-45	36.59004117057606	37.0	37.0	40.0	33.0	40.0
46-47	36.334381023957384	37.0	37.0	40.0	33.0	40.0
48-49	36.086736514905795	37.0	37.0	40.0	33.0	40.0
50-51	35.858617934417154	37.0	37.0	40.0	27.0	40.0
52-53	35.66817372730509	37.0	37.0	40.0	27.0	40.0
54-55	35.50659029178698	37.0	37.0	40.0	27.0	40.0
56-57	35.30888124151207	37.0	33.0	40.0	27.0	40.0
58-59	35.12186724549398	37.0	33.0	40.0	27.0	40.0
60-61	34.92807263030207	37.0	33.0	37.0	27.0	40.0
62-63	34.725439856018355	37.0	33.0	37.0	27.0	40.0
64-65	34.52373871023035	37.0	33.0	37.0	27.0	40.0
66-67	34.33385464050736	37.0	33.0	37.0	27.0	40.0
68-69	34.147333669554214	37.0	33.0	37.0	27.0	40.0
70-71	33.97177864140395	37.0	33.0	37.0	27.0	38.5
72-73	33.80450631567844	37.0	33.0	37.0	27.0	37.0
74-75	33.59940930606648	37.0	33.0	37.0	27.0	37.0
76-77	33.43517150561027	37.0	33.0	37.0	27.0	37.0
78-79	33.273051962606964	37.0	33.0	37.0	27.0	37.0
80-81	33.13348051237425	37.0	33.0	37.0	27.0	37.0
82-83	32.982413340717	37.0	33.0	37.0	27.0	37.0
84-85	32.841415094339624	37.0	33.0	37.0	24.5	37.0
86-87	32.72570052608678	37.0	33.0	37.0	22.0	37.0
88-89	32.6283763168651	37.0	33.0	37.0	22.0	37.0
90-91	32.483333432221826	37.0	33.0	37.0	22.0	37.0
92-93	32.35524267236264	37.0	33.0	37.0	22.0	37.0
94-95	32.247599943303925	37.0	33.0	37.0	22.0	37.0
96-97	32.18245523647535	37.0	33.0	37.0	22.0	37.0
98-99	32.04987971862927	35.0	33.0	37.0	22.0	37.0
100	31.93787719367641	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	9.0
7	235.0
8	1826.0
9	3482.0
10	3947.0
11	5299.0
12	9072.0
13	19743.0
14	33057.0
15	36671.0
16	40051.0
17	46520.0
18	56016.0
19	67041.0
20	81301.0
21	102630.0
22	129263.0
23	132462.0
24	116999.0
25	108859.0
26	112649.0
27	125904.0
28	151355.0
29	191984.0
30	250571.0
31	333706.0
32	449253.0
33	604517.0
34	833660.0
35	1273386.0
36	2535956.0
37	4977003.0
38	2330806.0
39	3367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27762321990402	19.913434394718706	12.572690860829624	24.23625152454765
2	16.73591340560257	21.04987690249982	37.557995325037524	24.656214366860084
3	17.66605355800799	27.56233271363211	29.45424099785082	25.31737273050908
4	12.412747386047492	17.385836530727953	37.68875176351146	32.51266431971309
5	13.667150603218733	38.24982129442209	32.80170238007596	15.28132572228322
6	30.193228115976424	38.46293000013185	17.578220798227917	13.765621085663806
7	26.714265446848213	32.632459853238885	22.580725335423267	18.072549364489635
8	27.250128555041336	32.068266023232205	19.835943989557375	20.845661432169084
9	27.73181440607571	13.954379441741494	20.23920467281094	38.074601479371864
10-11	25.832045468899345	25.540939321576616	26.017620569756232	22.609394639767803
12-13	26.443027042706646	24.078431760347033	26.33105889798663	23.147482298959694
14-15	24.576285880041667	24.54495470907005	24.38684848964308	26.491910921245204
16-17	23.6034604380101	26.63493005287238	25.208394314570892	24.55321519454663
18-19	23.83751961288451	25.688211173081232	27.792756088234903	22.68151312579935
20-21	25.822795116226942	24.663742863547064	26.12617512492913	23.387286895296864
22-23	24.997986771133274	24.437209822684558	26.116751979334435	24.448051426847734
24-25	24.024425522645902	25.45400317871448	26.444608909208327	24.076962389431287
26-27	24.437537258343024	25.174267272502227	26.266201868236532	24.121993600918213
28-29	23.492607220812385	25.2371696706576	26.981461815379753	24.28876129315026
30-31	24.325913400050105	25.049098136940785	26.601795815038965	24.02319264797015
32-33	23.830740758955784	25.245243819163537	25.815145625384268	25.108869796496414
34-35	24.486918729651393	25.11752503605068	26.92823219106948	23.467324043228448
36-37	24.514563594838243	25.028751017686645	26.14809013109884	24.308595256376275
38-39	24.785058898746843	24.309612096694124	26.38820564147955	24.51712336307948
40-41	24.22756119855914	24.798375380345835	26.975752173248196	23.998311247846825
42-43	24.89818770354548	24.697895652861835	26.377131047031366	24.026785596561318
44-45	24.145596825020107	25.263191725010874	26.02966325171736	24.56154819825165
46-47	23.7806488403676	25.001872288807142	26.39489142043432	24.82258745039094
48-49	25.078131165159572	25.085976420853267	25.558198183421155	24.277694230566
50-51	24.06808000231268	25.587415766840788	25.217867920217618	25.126636310628914
52-53	23.832519617928188	25.41860453233519	25.134142307261857	25.614733542474756
54-55	24.244536360938806	25.4286898875763	25.362820257823415	24.963953493661478
56-57	24.45914821282496	24.658003773133117	24.959451608802993	25.92339640523893
58-59	23.95179826922335	24.72005991428855	25.81836767186379	25.50977414462431
60-61	23.961919580857092	25.08457341600126	25.448512368874454	25.5049946342672
62-63	23.69436308887105	24.902535382120305	24.98693164868237	26.416169880326272
64-65	23.372653395660794	24.726931131507417	25.533615800315662	26.366799672516127
66-67	24.3196624811271	24.535866060975806	25.396873166851414	25.747598291045687
68-69	24.232407737035718	24.23130348219348	25.37784634046649	26.158442440304313
70-71	23.289001894409303	24.28971847431604	25.814594186977484	26.606685444297177
72-73	24.31905174414312	24.36904686560552	25.590214049133465	25.7216873411179
74-75	24.322598503369246	24.250765738721576	25.679104108853256	25.747531649055922
76-77	23.69241039089046	24.372036139504488	25.944547963629738	25.991005505975313
78-79	23.75311825098672	24.704294407988208	25.65808462813518	25.884502712889894
80-81	23.153236590568312	24.412755065627803	26.285724928741754	26.148283415062128
82-83	23.677554928890345	24.389211835731675	25.868305811889464	26.06492742348852
84-85	23.670521562139328	24.491319505163684	25.230934920574594	26.607224012122394
86-87	23.140639869361408	24.384257838496918	25.617993528062804	26.85710876407887
88-89	23.551792550658522	23.774193818478924	25.722261863219465	26.95175176764309
90-91	23.645011969332742	23.81944847553556	25.668757603928892	26.866781951202807
92-93	23.53374148191062	23.994495021208177	25.58218035068693	26.889583146194273
94-95	22.446247870360345	24.13652042962477	26.332414809057212	27.08481689095767
96-97	23.34180229593195	23.925365977422526	25.57503533056231	27.157796396083217
98-99	22.87965270361141	24.048821249159445	25.698788945584962	27.372737101644184
100	22.94450155856276	24.06902558743266	25.269944968712053	27.716527885292525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3771.0
1	2915.5
2	2506.5
3	2905.5
4	2265.5
5	1826.0
6	2308.0
7	4458.0
8	6965.0
9	6317.0
10	3643.5
11	2281.0
12	2380.5
13	2725.0
14	3260.0
15	4117.0
16	4985.0
17	5860.0
18	7201.5
19	8597.5
20	10043.0
21	11985.5
22	14208.0
23	17117.0
24	20896.5
25	25323.5
26	31747.0
27	38076.5
28	46511.5
29	65940.5
30	90557.5
31	112061.5
32	141304.0
33	170150.5
34	197995.0
35	221875.5
36	241012.0
37	279798.0
38	295657.0
39	286676.0
40	277220.0
41	267082.0
42	276532.5
43	324312.5
44	385901.0
45	445007.0
46	522069.0
47	592582.0
48	659574.5
49	713481.5
50	762031.5
51	755917.5
52	701409.0
53	686686.0
54	652024.0
55	602994.5
56	579079.5
57	526724.5
58	490789.0
59	466590.5
60	397007.0
61	316944.5
62	264441.0
63	208985.5
64	152082.0
65	126724.0
66	103626.5
67	71720.0
68	54406.5
69	48013.0
70	47169.0
71	46352.5
72	43279.0
73	50998.5
74	41872.5
75	29665.0
76	21168.5
77	13749.0
78	11037.5
79	7596.0
80	5039.5
81	3887.5
82	3912.0
83	3832.0
84	2957.5
85	2065.0
86	1590.0
87	859.0
88	367.5
89	248.0
90	195.5
91	144.0
92	93.0
93	73.5
94	55.5
95	36.5
96	23.0
97	14.5
98	11.5
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08217633796131482
2	8.965890062365676E-4
3	0.0
4	0.0
5	0.06320952493967802
6	0.0
7	2.6370264889310813E-5
8	0.0
9	0.0
10-11	5.142201653415608E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0011899582031301504
24-25	0.0013448835093548513
26-27	1.153699088907348E-4
28-29	0.003665466819614203
30-31	0.0
32-33	1.4503645689120946E-4
34-35	1.5492530622470102E-4
36-37	4.285168044513007E-5
38-39	1.7140672178052029E-4
40-41	2.307398177814696E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0012987355457985575
50-51	4.1862795511780914E-4
52-53	0.006806824624553354
54-55	6.592566222327703E-6
56-57	0.0020041401315876216
58-59	0.0017470300489168412
60-61	8.042930791239798E-4
62-63	0.0018129557111401184
64-65	0.001447068285800931
66-67	1.7140672178052029E-4
68-69	0.0
70-71	1.285550413353902E-4
72-73	7.911079466793244E-4
74-75	0.004815869625410387
76-77	0.006510159144548606
78-79	0.004232427514734386
80-81	0.006971638780111546
82-83	8.240707777909629E-4
84-85	0.00205358437825508
86-87	2.307398177814696E-4
88-89	0.0034347270018327334
90-91	2.867766306712551E-4
92-93	0.003378690188942948
94-95	0.0016316601400261066
96-97	4.977387497857416E-4
98-99	0.0
100	9.09774138681223E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.51686E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.00691589283373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.55074222896283	13.029381919372222
2	14.09646282319303	5.358605665337749
3	6.057464338264428	3.4540114560369517
4	3.095639185011037	2.3535421449626055
5	1.8631410037798348	1.770628217766657
6	1.1810960868756306	1.346939639076009
7	0.8212680284245568	1.0926840639217255
8	0.594967217817878	0.9046793494446157
9	0.4351481145338847	0.7443741252483653
>10	2.6139496481112223	9.4146738814428
>50	0.284483081630789	3.7824762694348077
>100	0.28073405908461424	11.46915711045882
>500	0.05492816374083354	7.2843086339836205
>1k	0.06206538493458317	24.69556589322007
>5k	0.005506081191160657	7.045831602437006
>10k+	0.002404554444241046	6.253140027855975
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	39042	0.25738697045211817	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	38961	0.25685297258810963	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31265	0.20611658294107565	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	24654	0.1625331276452672	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	19883	0.13107999419854172	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	19024	0.12541697981356223	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18309	0.12070329496459793	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	17866	0.11778278812810675	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	17361	0.11445354218583126	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	17050	0.11240325409068734	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	16347	0.10776868003639097	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	16306	0.10749838482127554	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA	16134	0.10636446343103517	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15761	0.10390543623010694	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC	15402	0.10153870495629128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.592566222327703E-6	0.0	0.0	0.0	0.0
6	1.977769866698311E-5	0.0	0.0	0.0	0.0
7	1.977769866698311E-5	0.0	0.0	0.0	0.0
8	1.977769866698311E-5	0.0	0.0	0.0	0.0
9	1.977769866698311E-5	0.0	0.0	1.3185132444655407E-5	0.0
10-11	1.977769866698311E-5	0.0	0.0	1.977769866698311E-5	0.0
12-13	3.6259114222802365E-5	3.2962831111638517E-6	0.0	5.274052977862163E-5	0.0
14-15	4.614796355629392E-5	6.592566222327703E-6	0.0	7.581451155676859E-5	6.592566222327703E-6
16-17	4.614796355629392E-5	6.592566222327703E-6	0.0	9.888849333491555E-5	1.3185132444655407E-5
18-19	5.6036812889785476E-5	6.592566222327703E-6	0.0	1.0877734266840711E-4	1.977769866698311E-5
20-21	6.922194533444089E-5	6.592566222327703E-6	0.0	2.3733238400379733E-4	2.6370264889310813E-5
22-23	7.251822844560473E-5	6.592566222327703E-6	0.0	8.471447595691098E-4	2.6370264889310813E-5
24-25	1.0548105955724325E-4	6.592566222327703E-6	0.0	0.002637026488931081	2.6370264889310813E-5
26-27	1.4833274000237333E-4	6.592566222327703E-6	0.0	0.004627981488074048	2.6370264889310813E-5
28-29	1.7470300489168413E-4	6.592566222327703E-6	0.0	0.019078886647416374	2.6370264889310813E-5
30-31	1.8459185422517568E-4	6.592566222327703E-6	0.0	0.07455203512519283	3.2962831111638516E-5
32-33	1.911844204475034E-4	6.592566222327703E-6	0.0	0.17547103885658533	3.955539733396622E-5
34-35	2.043695528921588E-4	6.592566222327703E-6	0.0	0.3047150033622088	3.955539733396622E-5
36-37	2.0766583600332266E-4	6.592566222327703E-6	0.0	0.5080890787547961	3.955539733396622E-5
38-39	2.109621191144865E-4	6.592566222327703E-6	0.0	0.8553492082327967	3.955539733396622E-5
40-41	2.109621191144865E-4	6.592566222327703E-6	0.0	1.2740332001634957	3.955539733396622E-5
42-43	2.1425840222565035E-4	6.592566222327703E-6	0.0	1.6239863929433171	3.955539733396622E-5
44-45	2.3073981778146962E-4	6.592566222327703E-6	0.0	2.013363131732658	3.955539733396622E-5
46-47	2.472212333372889E-4	6.592566222327703E-6	0.0	2.4319119760557992	4.614796355629392E-5
48-49	2.505175164484527E-4	6.592566222327703E-6	0.0	2.8769464551771424	4.614796355629392E-5
50-51	2.5711008267078044E-4	6.592566222327703E-6	0.0	3.3657786479965193	4.614796355629392E-5
52-53	2.6370264889310813E-4	6.592566222327703E-6	0.0	3.816772806982846	4.614796355629392E-5
54-55	2.6370264889310813E-4	6.592566222327703E-6	0.0	4.240266075972733	4.614796355629392E-5
56-57	2.735914982265997E-4	6.592566222327703E-6	0.0	4.715171472647443	4.614796355629392E-5
58-59	2.900729137824189E-4	6.592566222327703E-6	0.0	5.258988964044144	4.614796355629392E-5
60-61	2.900729137824189E-4	6.592566222327703E-6	0.0	5.827307727806126	4.614796355629392E-5
62-63	3.131468955605659E-4	6.592566222327703E-6	0.0	6.394947457247208	4.614796355629392E-5
64-65	3.164431786717298E-4	6.592566222327703E-6	0.0	6.935649961103859	4.614796355629392E-5
66-67	3.2303574489405746E-4	6.592566222327703E-6	0.0	7.502488693748929	6.262937911211318E-5
68-69	3.2962831111638515E-4	6.592566222327703E-6	0.0	8.138226335983545	7.251822844560473E-5
70-71	3.362208773387129E-4	6.592566222327703E-6	0.0	8.86594675843519	7.251822844560473E-5
72-73	3.362208773387129E-4	6.592566222327703E-6	0.0	9.584249700038237	7.251822844560473E-5
74-75	3.362208773387129E-4	6.592566222327703E-6	0.0	10.254097279907176	7.251822844560473E-5
76-77	3.362208773387129E-4	6.592566222327703E-6	0.0	10.956182508603298	7.251822844560473E-5
78-79	3.395171604498767E-4	6.592566222327703E-6	0.0	11.684881927138957	7.251822844560473E-5
80-81	3.527022928945321E-4	9.888849333491555E-6	0.0	12.49705312289862	7.581451155676859E-5
82-83	3.55998576005696E-4	2.6370264889310813E-5	0.0	13.358381788695068	7.911079466793244E-5
84-85	3.625911422280237E-4	3.6259114222802365E-5	0.0	14.163907677702623	8.240707777909629E-5
86-87	3.625911422280237E-4	3.955539733396622E-5	0.0	14.981069446092587	8.570336089026014E-5
88	3.625911422280237E-4	3.955539733396622E-5	0.0	15.604624025948342	8.570336089026014E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAG	3975	0.0	30.623747	1
CTATTGA	9765	0.0	27.818157	9
AGGGCTA	10775	0.0	25.60316	5
GTACTAG	7505	0.0	25.425589	1
GTATAAG	6960	0.0	24.44528	1
GTATCAA	86720	0.0	24.301998	1
GTGATCG	5780	0.0	23.986504	8
TTTAGGG	11690	0.0	23.639898	2
ATGGGAG	16105	0.0	23.578892	5
ATTTAGG	11875	0.0	23.509775	1
CGTTAAC	2090	0.0	23.38744	1
TAGTACT	8375	0.0	23.288206	4
TAGGCAT	10300	0.0	23.22487	5
GGGCTAT	12540	0.0	23.123861	6
ACGTTAA	1515	0.0	22.955769	8
GGCTATT	12775	0.0	22.440973	7
TATTCGC	1230	0.0	22.161322	9
TAAGGTG	6765	0.0	22.16132	4
CTGTGCG	8975	0.0	21.783697	9
TATTCTC	6110	0.0	21.767973	5
>>END_MODULE