FastQCFastQC Report
Fri 27 May 2016
ERR765305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7631562
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA456040.5975709821921121No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT418350.5481839759671742No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG243000.3184145002032349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230480.30200894653021226No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA221940.2908185768522879No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC214390.28092545143445075No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG185150.24261088359106564No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG176000.2306212017932895No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT149590.19601491804692148No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC149520.19592319370529912No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT148390.1944425007619672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142210.18634455174445286No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC130990.17164245013013063No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC114520.15006102289413362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111510.14611687620437336No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA91800.12028992229899986No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG89550.11734163988971066No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG80610.10562713111680151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC57150.022.246551
TACACTC69900.020.8643765
TCTACAC74300.020.5773623
AGTACTT244250.020.45347812-13
GAGTACT233650.019.21911412-13
ATCTACA68100.019.0681482
CTACACT91950.018.4199624
CATGGGG148700.018.1609654
TACTTTT288350.017.50048614-15
GTACTTT266000.016.97491514-15
GTACTAG17950.016.5268941
GTATTAG34350.016.3130571
GTATAAG24300.016.0837361
CACTCTT89000.015.7391667
CATGGGT68200.015.4526024
ACTTTTT305950.015.02965216-17
GTATAAA39100.014.210861
ACTCTTT122500.014.19780358
ATTGCGT11750.014.0019516
TGCGCGA12850.013.92904694
Produced by FastQC (version 0.11.5)