##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765305_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7631562 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.249604340500674 33.0 31.0 34.0 31.0 34.0 2 32.29410623932558 34.0 31.0 34.0 31.0 34.0 3 32.32521166702177 34.0 31.0 34.0 30.0 34.0 4 35.77874699832092 37.0 35.0 37.0 35.0 37.0 5 35.789278787225996 37.0 35.0 37.0 35.0 37.0 6 35.87176950144676 37.0 37.0 37.0 35.0 37.0 7 35.82516370829458 37.0 37.0 37.0 35.0 37.0 8 35.84627524483192 37.0 37.0 37.0 35.0 37.0 9 37.55755414160299 39.0 38.0 39.0 35.0 39.0 10-11 37.481057613107254 39.0 38.0 39.0 35.0 39.0 12-13 37.45598882640277 39.0 38.0 39.0 35.0 39.0 14-15 38.861457392339865 41.0 39.0 41.0 35.5 41.0 16-17 38.820024983089965 41.0 39.0 41.0 35.0 41.0 18-19 38.79998806273211 41.0 39.0 41.0 35.0 41.0 20-21 38.76467609121173 41.0 39.0 41.0 35.0 41.0 22-23 38.66598784102128 40.5 39.0 41.0 34.5 41.0 24-25 38.585440831116884 40.5 38.0 41.0 34.0 41.0 26-27 38.469933284955296 40.0 38.0 41.0 34.0 41.0 28-29 38.333020815397944 40.0 38.0 41.0 34.0 41.0 30-31 38.1357497980099 40.0 38.0 41.0 34.0 41.0 32-33 37.9308870844527 40.0 38.0 41.0 33.0 41.0 34-35 37.77264411925108 40.0 38.0 41.0 33.0 41.0 36-37 37.61797021369937 40.0 37.0 41.0 33.0 41.0 38-39 37.43561639150674 40.0 37.0 41.0 32.5 41.0 40-41 37.217011537087686 40.0 37.0 41.0 32.0 41.0 42-43 36.98301691056169 40.0 36.0 41.0 31.0 41.0 44-45 36.683119916997335 40.0 36.0 41.0 31.0 41.0 46-47 36.30607783832458 39.5 35.0 41.0 30.0 41.0 48-49 35.956412396308906 39.0 35.0 41.0 29.0 41.0 50-51 34.67106072125208 38.0 34.0 40.0 26.5 40.5 52-53 34.6071529000223 38.0 34.0 39.5 26.5 40.0 54-55 35.44530896820336 39.0 35.0 40.5 28.0 41.0 56-57 35.473608286219786 39.0 35.0 41.0 28.0 41.0 58-59 35.26164774655569 38.0 35.0 41.0 28.0 41.0 60-61 34.94535155450483 37.5 35.0 40.0 28.0 41.0 62-63 34.61105655696697 37.0 35.0 40.0 27.0 41.0 64-65 34.29537924215252 36.5 34.5 39.5 26.5 41.0 66-67 33.99585012871546 36.0 34.0 39.0 26.0 41.0 68-69 33.766001507948175 35.5 34.0 39.0 26.0 41.0 70-71 33.45471864082346 35.0 34.0 38.5 26.0 41.0 72-73 33.11851066662369 35.0 34.0 37.0 26.0 39.5 74-75 32.76805501940494 35.0 34.0 37.0 25.5 39.0 76-77 32.42940737427017 35.0 34.0 36.0 25.0 39.0 78-79 32.10273689711228 35.0 34.0 36.0 24.0 37.5 80-81 31.782412958186015 35.0 33.5 35.5 23.0 37.0 82-83 31.515079678314873 35.0 33.0 35.0 20.5 37.0 84-85 31.251261471766853 35.0 33.0 35.0 19.5 36.0 86-87 31.002640285173598 35.0 33.0 35.0 18.0 36.0 88-89 30.76045270941912 35.0 33.0 35.0 12.5 36.0 90-91 30.509056533904854 35.0 33.0 35.0 4.5 35.5 92-93 30.23038678058306 35.0 32.5 35.0 2.0 35.0 94-95 29.882341845614306 35.0 32.0 35.0 2.0 35.0 96-97 29.559699836023086 35.0 32.0 35.0 2.0 35.0 98-99 29.245932811657692 35.0 31.5 35.0 2.0 35.0 100 28.695411633948595 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42424636063696397 1101 2 0.5003668425088321 1101 3 0.4884295037708881 1101 4 0.47265160940531814 1101 5 0.5209432876712228 1101 6 0.4675812756090352 1101 7 0.48780516347121505 1101 8 0.45345407375668145 1101 9 0.5513826182187529 1101 10-11 0.4608998158214703 1101 12-13 0.5077245550818645 1101 14-15 0.6748399480925684 1101 16-17 0.7319005139674388 1101 18-19 0.6989477427906152 1101 20-21 0.700087853020392 1101 22-23 0.7304288090871509 1101 24-25 0.6909815958550993 1101 26-27 0.7368219424583557 1101 28-29 0.7369637522302384 1101 30-31 0.688099042825435 1101 32-33 0.6989249090864931 1101 34-35 0.7830377912059205 1101 36-37 0.7344243132263344 1101 38-39 0.7966533516791827 1101 40-41 0.8062889207880133 1101 42-43 0.7392128570990693 1101 44-45 0.724820152028947 1101 46-47 0.718619233004425 1101 48-49 0.7377632974722772 1101 50-51 0.7024389244943237 1101 52-53 0.818368065633301 1101 54-55 0.8862246723260441 1101 56-57 0.8824232295352346 1101 58-59 0.7854664847442336 1101 60-61 0.8278644137291593 1101 62-63 0.7852601754037138 1101 64-65 0.7127355827274684 1101 66-67 0.6884401235920805 1101 68-69 0.6909484311479943 1101 70-71 0.6744408245070233 1101 72-73 0.6754040427220502 1101 74-75 0.5750780023574436 1101 76-77 0.6109594081753542 1101 78-79 0.5901101079520998 1101 80-81 0.5580781696503152 1101 82-83 0.5886056869506433 1101 84-85 0.6097295671946767 1101 86-87 0.48178134336540523 1101 88-89 0.5393645553133872 1101 90-91 0.575842899147812 1101 92-93 0.5607510549836974 1101 94-95 0.5594127408205658 1101 96-97 0.4729089345862647 1101 98-99 0.5467069525253336 1101 100 0.6408441850431927 1102 1 0.5240588548450944 1102 2 0.5797620711692204 1102 3 0.5669302196243251 1102 4 0.47434304400101723 1102 5 0.4454250345887871 1102 6 0.47047309975626206 1102 7 0.5206899605501363 1102 8 0.4337216333663818 1102 9 0.4849800414496528 1102 10-11 0.501627614787516 1102 12-13 0.5452181173219728 1102 14-15 0.6771219785819937 1102 16-17 0.6278912483425714 1102 18-19 0.6190340028364361 1102 20-21 0.7271701759795945 1102 22-23 0.7403194055788518 1102 24-25 0.7098894336269552 1102 26-27 0.6839573799944318 1102 28-29 0.6724214130493493 1102 30-31 0.6157258612950116 1102 32-33 0.7437019914309175 1102 34-35 0.7712487332619489 1102 36-37 0.7025576842990588 1102 38-39 0.7409641715475885 1102 40-41 0.7466876740834962 1102 42-43 0.7721098852422656 1102 44-45 0.7077906337929676 1102 46-47 0.659249232094318 1102 48-49 0.7251682481550503 1102 50-51 0.6888897091092332 1102 52-53 0.7077295202481722 1102 54-55 0.7911747685823798 1102 56-57 0.7768136686252944 1102 58-59 0.7773642987368987 1102 60-61 0.7133259040873483 1102 62-63 0.6770595171693188 1102 64-65 0.7497498016901858 1102 66-67 0.6857770704157318 1102 68-69 0.7141510319042865 1102 70-71 0.6993230293441997 1102 72-73 0.7315631675237384 1102 74-75 0.7011451841746066 1102 76-77 0.6563969250753203 1102 78-79 0.6305884790386926 1102 80-81 0.6444142626455402 1102 82-83 0.6864721115404606 1102 84-85 0.6234879552371027 1102 86-87 0.6603141117599485 1102 88-89 0.6410501189982192 1102 90-91 0.6587544371957073 1102 92-93 0.6822494264496761 1102 94-95 0.7272793058888105 1102 96-97 0.7385413434104038 1102 98-99 0.7590734197021511 1102 100 0.8150972539745815 1103 1 0.5091498858856411 1103 2 0.6197156065113276 1103 3 0.6251187751158795 1103 4 0.4970720784028302 1103 5 0.5602076846638653 1103 6 0.5393771580289126 1103 7 0.5566386755451305 1103 8 0.5151177155504243 1103 9 0.6292824713083363 1103 10-11 0.5802119871533549 1103 12-13 0.6299996141188373 1103 14-15 0.7714417740011115 1103 16-17 0.7543031146114743 1103 18-19 0.7498310747578429 1103 20-21 0.7677726460768852 1103 22-23 0.7749341098787639 1103 24-25 0.7140966419530557 1103 26-27 0.7617630704863743 1103 28-29 0.7647713014617779 1103 30-31 0.7705774541984809 1103 32-33 0.8033718707860018 1103 34-35 0.7639549721625016 1103 36-37 0.7802173628114062 1103 38-39 0.7871672506437619 1103 40-41 0.8160454887808726 1103 42-43 0.7544620730413172 1103 44-45 0.7471611170245112 1103 46-47 0.7020854982675289 1103 48-49 0.699485979729296 1103 50-51 0.6553238825352494 1103 52-53 0.7447700256469219 1103 54-55 0.8597750789185739 1103 56-57 0.8273494145539857 1103 58-59 0.8504307938165852 1103 60-61 0.7821882313741071 1103 62-63 0.81458021702624 1103 64-65 0.7889841513555922 1103 66-67 0.7363220176085719 1103 68-69 0.747195015354329 1103 70-71 0.7070391191739205 1103 72-73 0.6364700144831303 1103 74-75 0.6321740482879719 1103 76-77 0.6923484150547452 1103 78-79 0.7012026314220918 1103 80-81 0.6396881587866119 1103 82-83 0.5664348492089175 1103 84-85 0.5851192413834347 1103 86-87 0.5693209251831917 1103 88-89 0.5340938544257341 1103 90-91 0.5244323782390516 1103 92-93 0.6089220828339208 1103 94-95 0.5231928903776186 1103 96-97 0.5690606300965406 1103 98-99 0.611578682280868 1103 100 0.5773328440978567 1104 1 0.49799667039471274 1104 2 0.543359414579129 1104 3 0.5354645933188422 1104 4 0.5233417077717633 1104 5 0.5155241839873952 1104 6 0.5131226356504399 1104 7 0.5022669929934906 1104 8 0.45244540605375505 1104 9 0.5223290385326251 1104 10-11 0.5665862813936329 1104 12-13 0.5504752722300239 1104 14-15 0.6872129209090616 1104 16-17 0.656904029061721 1104 18-19 0.6816773635411053 1104 20-21 0.6816861621487078 1104 22-23 0.6880968282079962 1104 24-25 0.6534898812451289 1104 26-27 0.6692578010007892 1104 28-29 0.6562624760760514 1104 30-31 0.6591380325905263 1104 32-33 0.7093479058830425 1104 34-35 0.7312545011688627 1104 36-37 0.7256881640497639 1104 38-39 0.7510478265871896 1104 40-41 0.7353318650606724 1104 42-43 0.7271106207674549 1104 44-45 0.7694863702339063 1104 46-47 0.7454511723704726 1104 48-49 0.7781313851030021 1104 50-51 0.7172402151012207 1104 52-53 0.7531822327250879 1104 54-55 0.8038572918495888 1104 56-57 0.7934298168113187 1104 58-59 0.8033039711729444 1104 60-61 0.7985510457778915 1104 62-63 0.7722442663945017 1104 64-65 0.7529031959954224 1104 66-67 0.7435158852727142 1104 68-69 0.7253834982375196 1104 70-71 0.6747488989277812 1104 72-73 0.651440033258119 1104 74-75 0.6080852015674338 1104 76-77 0.6452223920482254 1104 78-79 0.704152689581548 1104 80-81 0.7156518273110919 1104 82-83 0.7287131565843055 1104 84-85 0.7302486172560805 1104 86-87 0.794348762396563 1104 88-89 0.6755207382157771 1104 90-91 0.5595420205438977 1104 92-93 0.6317223327931032 1104 94-95 0.6003319639539768 1104 96-97 0.569287345672457 1104 98-99 0.577368078138047 1104 100 0.6585406435565453 1105 1 0.33254185405856873 1105 2 0.3783216847815183 1105 3 0.41217383031004573 1105 4 0.40790749686180305 1105 5 0.40033211794762735 1105 6 0.42437342946604417 1105 7 0.386816070176188 1105 8 0.4384213601081015 1105 9 0.3996613274310121 1105 10-11 0.49350711068826314 1105 12-13 0.4642006283892144 1105 14-15 0.5014339806857748 1105 16-17 0.5770030222226339 1105 18-19 0.6285552770132483 1105 20-21 0.5663708902388578 1105 22-23 0.5630649600229916 1105 24-25 0.5788549919739623 1105 26-27 0.5188350786381974 1105 28-29 0.5390527583508344 1105 30-31 0.5312442058268658 1105 32-33 0.5441251450475022 1105 34-35 0.5623661434657734 1105 36-37 0.55670397955884 1105 38-39 0.5222686598642241 1105 40-41 0.5459488420117466 1105 42-43 0.48840720343130073 1105 44-45 0.5007209872787755 1105 46-47 0.4176473765629609 1105 48-49 0.4364995082420151 1105 50-51 0.3590588386374165 1105 52-53 0.33219981969634205 1105 54-55 0.42439467923088614 1105 56-57 0.4241519116595711 1105 58-59 0.47186902458349067 1105 60-61 0.41648199786592244 1105 62-63 0.36208908033407994 1105 64-65 0.3857049454832193 1105 66-67 0.32957971906959216 1105 68-69 0.30296768358181936 1105 70-71 0.3140660699503215 1105 72-73 0.3354596452147476 1105 74-75 0.35159671464929687 1105 76-77 0.338002376943507 1105 78-79 0.35251975844811767 1105 80-81 0.4158162084762722 1105 82-83 0.3537764040421365 1105 84-85 0.3199534689716863 1105 86-87 0.3233387658313944 1105 88-89 0.2792855931457545 1105 90-91 0.3200811448968466 1105 92-93 0.31893507466240223 1105 94-95 0.3003991849344594 1105 96-97 0.34896275954106315 1105 98-99 0.33936313852492717 1105 100 0.3900941646843066 1106 1 0.22812739665472748 1106 2 0.27753692328866464 1106 3 0.33261074702387106 1106 4 0.3482595048489472 1106 5 0.29738643777783125 1106 6 0.3078801650576537 1106 7 0.29129359079475137 1106 8 0.326135493390332 1106 9 0.3435886441624447 1106 10-11 0.34396716784657855 1106 12-13 0.40954265892113284 1106 14-15 0.47496863502296094 1106 16-17 0.4790609613893011 1106 18-19 0.4877121011134946 1106 20-21 0.4985773678799035 1106 22-23 0.4833077987223007 1106 24-25 0.5364635137955176 1106 26-27 0.4649043756581648 1106 28-29 0.5042461856617422 1106 30-31 0.5251831122045374 1106 32-33 0.5040406701232243 1106 34-35 0.4097866702184305 1106 36-37 0.45393602888023565 1106 38-39 0.49807308814503415 1106 40-41 0.47012487643623757 1106 42-43 0.47332803773044674 1106 44-45 0.5151178853973732 1106 46-47 0.5568465546692778 1106 48-49 0.5659798938988274 1106 50-51 0.47445157225295986 1106 52-53 0.5698001931548831 1106 54-55 0.5981874136233927 1106 56-57 0.6351853681841462 1106 58-59 0.5958100934985069 1106 60-61 0.5986742312490207 1106 62-63 0.5009038253424407 1106 64-65 0.5401106782790706 1106 66-67 0.5525378046931237 1106 68-69 0.505687268530032 1106 70-71 0.5562751892537605 1106 72-73 0.5128666981943226 1106 74-75 0.5683817759190362 1106 76-77 0.5526877050988404 1106 78-79 0.5894438533873938 1106 80-81 0.6890503737174356 1106 82-83 0.6074629960516233 1106 84-85 0.573398636995659 1106 86-87 0.5906816354795978 1106 88-89 0.6379740563327623 1106 90-91 0.6118504889228689 1106 92-93 0.5890474812815789 1106 94-95 0.5311909649616808 1106 96-97 0.5585743801361893 1106 98-99 0.5554991957920201 1106 100 0.5557772221754291 1107 1 0.25767137815648766 1107 2 0.29650703881510054 1107 3 0.34923026302152493 1107 4 0.3047246936413899 1107 5 0.32998170986493136 1107 6 0.31199015603625213 1107 7 0.3475262377428052 1107 8 0.291559674238691 1107 9 0.35944287414676523 1107 10-11 0.41383756189771503 1107 12-13 0.4161362712462875 1107 14-15 0.42299870780724547 1107 16-17 0.47459022399954875 1107 18-19 0.45647222828892353 1107 20-21 0.453704127271358 1107 22-23 0.3851864171347117 1107 24-25 0.42545105901198355 1107 26-27 0.4959395153146531 1107 28-29 0.5091105143559105 1107 30-31 0.4708467104282832 1107 32-33 0.45564426729593066 1107 34-35 0.4816737407167082 1107 36-37 0.5531993179240544 1107 38-39 0.5028033001916583 1107 40-41 0.451477188278794 1107 42-43 0.4636371519693583 1107 44-45 0.44948557740861617 1107 46-47 0.44061121443997564 1107 48-49 0.47994707528146563 1107 50-51 0.5277922263480619 1107 52-53 0.5237598640052639 1107 54-55 0.5191056692530083 1107 56-57 0.5132957795773834 1107 58-59 0.45831425385165403 1107 60-61 0.4753454889018798 1107 62-63 0.5260318109432447 1107 64-65 0.5268030223534126 1107 66-67 0.5161303353264586 1107 68-69 0.5034029062026875 1107 70-71 0.46210231125282775 1107 72-73 0.5374740922366996 1107 74-75 0.46655877485923725 1107 76-77 0.4686836060561532 1107 78-79 0.42265420084257244 1107 80-81 0.4870783663251501 1107 82-83 0.453955818347616 1107 84-85 0.44720034580597456 1107 86-87 0.5081993498253716 1107 88-89 0.4923534849192066 1107 90-91 0.479560564860261 1107 92-93 0.5283424546991142 1107 94-95 0.4252880807421171 1107 96-97 0.48018018886504876 1107 98-99 0.5788081217202183 1107 100 0.5828797332770002 1108 1 0.22006999778586334 1108 2 0.23944571376532764 1108 3 0.3142887181498111 1108 4 0.26267672597708014 1108 5 0.2721078373980532 1108 6 0.29311838048904804 1108 7 0.3193266442314382 1108 8 0.3316005953359067 1108 9 0.37449538310721664 1108 10-11 0.31810975811838915 1108 12-13 0.3782842025888229 1108 14-15 0.3985201258321567 1108 16-17 0.3805952445711398 1108 18-19 0.3421863630462809 1108 20-21 0.3880899382820857 1108 22-23 0.3776632611094186 1108 24-25 0.40473036555986397 1108 26-27 0.3826330304813226 1108 28-29 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2316 70-71 -0.9905113896714752 2316 72-73 -1.006045830665947 2316 74-75 -0.9867814072630239 2316 76-77 -1.0246680870511717 2316 78-79 -0.9094284346824537 2316 80-81 -0.8043015999975225 2316 82-83 -0.7383017892840869 2316 84-85 -0.778299152798791 2316 86-87 -0.8143224657159394 2316 88-89 -0.7996717639530821 2316 90-91 -0.728390243076273 2316 92-93 -0.6822686285883925 2316 94-95 -0.560375937105043 2316 96-97 -0.7176815087599699 2316 98-99 -0.49139809204413254 2316 100 -0.5669039081187357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22755.0 3 3609.0 4 3530.0 5 4190.0 6 5095.0 7 5931.0 8 7311.0 9 8774.0 10 10906.0 11 14637.0 12 20356.0 13 27820.0 14 29120.0 15 34965.0 16 44991.0 17 60848.0 18 62860.0 19 38294.0 20 33058.0 21 31688.0 22 33732.0 23 38882.0 24 44010.0 25 49592.0 26 57278.0 27 68014.0 28 81682.0 29 98635.0 30 119759.0 31 148184.0 32 187939.0 33 250142.0 34 362841.0 35 586342.0 36 1077855.0 37 1874609.0 38 1697333.0 39 383492.0 40 503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.339579585219354 18.15047608206012 13.281720290215826 24.228224042504696 2 15.755546090839287 21.342552626969088 37.22835610888372 25.673545173307904 3 18.36691914894176 26.352197331752773 29.632513110920257 25.648370408385208 4 13.44341560040434 17.680478406126046 35.50916082435393 33.36694516911568 5 14.334083315607039 36.352114968417396 33.202281446602484 16.111520269373088 6 31.386023907365455 35.20206378430288 17.909259332669837 15.502652975661826 7 26.765544298070736 31.895178087522353 22.061237840780773 19.278039773626134 8 27.216843433703797 31.35208048675911 20.969273862893807 20.461802216643292 9 27.555630138756264 14.623940472101154 19.776142724934004 38.04428666420857 10-11 25.288457503677563 24.994758064224857 26.757775549011097 22.95900888308648 12-13 26.820461840019778 23.136971502867684 26.785454853071798 23.25711180404074 14-15 24.91859972961413 23.484139622227666 24.974714084237032 26.622546563921173 16-17 23.516810063437223 26.28445479608977 26.199918206485574 23.998816933987435 18-19 24.227807806033873 25.270776107353477 28.00131927420228 22.500096812410366 20-21 24.983536599698215 24.71201360090249 27.12734379401046 23.177106005388833 22-23 23.992796229309096 25.052687746140588 26.326585595805852 24.627930428744463 24-25 23.695223972622884 25.735161275413954 26.104809849627536 24.464804902335626 26-27 24.135186971767926 25.091505957373283 26.29169859320176 24.481608477657026 28-29 23.642042953248602 24.663544530820705 27.693948647742804 24.00046386818789 30-31 24.58035584783582 24.596278979574247 27.092604309650785 23.73076086293915 32-33 23.104119500151167 25.22400236827458 26.992728640545778 24.67914949102848 34-35 23.399459779718793 25.442140239809614 27.302581824579136 23.855818155892457 36-37 24.011925209701975 25.41028832795429 26.504012153551383 24.073774308792355 38-39 24.27928426385517 24.61005008321621 26.550211420269438 24.56045423265918 40-41 23.79807526761053 25.16972371250916 26.71820396963512 24.3139970502452 42-43 24.748377196834955 24.89527292015749 26.44687826744258 23.90947161556498 44-45 24.32710115310599 24.870161089476394 26.55449783243157 24.248239924986045 46-47 23.772541860219 24.933672026388855 27.250614566129837 24.04317154726231 48-49 24.221818936499176 25.524707867159602 26.58654891908272 23.666924277258506 50-51 23.439660408206144 25.950223407014423 25.895049589462936 24.715066595316497 52-53 23.405138001749986 25.762666803838215 25.427838755546126 25.404356438865666 54-55 23.851405233517706 25.430743324657996 25.505151135989635 25.212700305834662 56-57 24.037722021004175 24.761096753387665 25.57856202523408 25.622619200374082 58-59 23.895614608915654 24.650698157515045 26.524657777824434 24.929029455744864 60-61 23.838406684853783 25.006179281015072 26.137289455102863 25.018124579028285 62-63 22.90348207157513 25.216366455503188 26.335328179957685 25.544823292963997 64-65 23.172927297280047 25.045964000327185 26.25686590227157 25.524242800121193 66-67 23.987393665043797 25.08496399234893 25.663097639992237 25.264544702615037 68-69 24.182356872977063 24.598984214243078 25.66736489339116 25.5512940193887 70-71 23.581948922797427 24.921134736083715 25.858910543090957 25.6380057980279 72-73 24.444036571543766 24.868960805178727 25.675728882143602 25.011273741133905 74-75 24.715347531541454 24.626035142406487 25.739699255218046 24.918918070834014 76-77 24.10339954136157 25.22898612211486 26.080694451491 24.586919885032568 78-79 24.179968969554306 25.201513090382527 26.071916238673538 24.546601701389633 80-81 23.851159413141872 25.141443995890604 25.9308242710184 25.076572319949126 82-83 24.098124382165746 25.45306533056339 25.18093940070356 25.267870886567305 84-85 24.20476829881896 25.399639036132747 25.063852588962078 25.331740076086216 86-87 24.292892191212843 24.80090556222455 25.398325085364164 25.50787716119844 88-89 24.51642725121342 24.34213747417911 25.819774289619517 25.321660984987947 90-91 24.955184061862106 24.745978477979662 25.33342775752157 24.96540970263666 92-93 24.57848918732491 24.8306160195724 25.413602739191344 25.177292053911348 94-95 24.376236609405325 24.81830402536027 25.73648805000247 25.068971315231934 96-97 25.20950298807132 24.651422366138476 25.061278132128773 25.077796513661433 98-99 25.240531896787715 24.389975452243387 25.00458712065466 25.364905530314243 100 25.259288762087177 24.6062725254603 25.04550830705939 25.088930405393135 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2759.0 1 3618.5 2 2908.0 3 1203.0 4 985.5 5 1033.5 6 1376.5 7 2272.5 8 3550.0 9 3815.0 10 2985.0 11 2631.0 12 2887.0 13 3138.0 14 3476.0 15 3894.0 16 4314.5 17 4772.0 18 5371.0 19 6100.0 20 7002.0 21 8240.5 22 10004.5 23 12133.5 24 14599.5 25 17721.0 26 22125.0 27 27652.0 28 33263.0 29 40975.0 30 50101.5 31 58056.0 32 66574.5 33 76338.0 34 86625.0 35 94862.5 36 103045.5 37 113872.0 38 123355.0 39 132450.5 40 139680.5 41 147262.5 42 157674.5 43 176210.0 44 200575.5 45 222230.5 46 251796.0 47 285071.0 48 352307.0 49 421000.5 50 466604.0 51 440846.0 52 367268.0 53 341433.0 54 317464.0 55 290147.5 56 269012.0 57 244531.5 58 229070.5 59 210510.0 60 179737.5 61 150233.5 62 124692.5 63 98077.0 64 73846.5 65 59840.0 66 47326.0 67 35695.5 68 28227.5 69 23440.0 70 20488.0 71 17965.5 72 15169.0 73 15032.0 74 12711.5 75 10033.0 76 7808.5 77 5364.5 78 3940.5 79 2759.5 80 1960.0 81 1543.0 82 1205.0 83 869.0 84 600.0 85 380.5 86 280.0 87 197.0 88 138.0 89 108.5 90 94.0 91 83.5 92 70.0 93 63.0 94 50.5 95 39.0 96 34.0 97 25.0 98 15.5 99 9.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08669260631047747 2 0.2884468474474819 3 0.29413375662806646 4 0.3030965351523056 5 0.32968349074540704 6 0.17459073253941984 7 0.1913762870563064 8 0.15754310847504088 9 0.2084239111206854 10-11 0.22912085363389567 12-13 0.2462929607333335 14-15 0.17284141830990826 16-17 0.1621686359882813 18-19 0.18000246869513736 20-21 0.21207978130820404 22-23 0.17466280166498024 24-25 0.20014251341992634 26-27 0.19809837094948582 28-29 0.22708326290214242 30-31 0.26199092662812673 32-33 0.23035913224579713 34-35 0.15583310467765316 36-37 0.14941240076409 38-39 0.11725646728677563 40-41 0.10766472184855472 42-43 0.12433234506906973 44-45 0.13863479062346606 46-47 0.20467631659154442 48-49 0.2104746053298132 50-51 0.2166922053440698 52-53 0.1432734216140811 54-55 0.08766881537488655 56-57 0.12642234971032143 58-59 0.14606446229487488 60-61 0.122071995221948 62-63 0.14316859379508415 64-65 0.11819991765774818 66-67 0.12268130691986778 68-69 0.10748127316531006 70-71 0.13336064098018202 72-73 0.15836862754964187 74-75 0.197043541020829 76-77 0.17792556753126032 78-79 0.20301872670365514 80-81 0.1054829928656807 82-83 0.14666722225410736 84-85 0.17853487922918007 86-87 0.19178904659360693 88-89 0.1731624535055864 90-91 0.17634004676893145 92-93 0.1258523484445255 94-95 0.36503012096344106 96-97 0.36573770874167044 98-99 0.41247781253693544 100 0.44614719765101823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7631562.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.122394554088615 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75368211848077 32.751712762278316 2 12.017768795598466 10.124343977360258 3 3.837862046131197 4.849798180539003 4 1.7660393621771984 2.975592272467159 5 1.080390836258572 2.275432453875266 6 0.6447327985669971 1.6294613593920475 7 0.45138687318241905 1.3309447178108356 8 0.3289284762748339 1.1084204046178987 9 0.2625528328076099 0.9953418613334226 >10 1.5912376283023615 12.51448372945338 >50 0.13688427441856474 3.986118463787363 >100 0.10428177172970107 8.857449174392992 >500 0.014420328177543275 4.232285147636398 >1k 0.008895490476036876 7.037366716803743 >5k 4.6818370886132684E-4 1.2659842351551942 >10k+ 4.6818370886132684E-4 4.065264543096698 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 45604 0.5975709821921121 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 41835 0.5481839759671742 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 24300 0.3184145002032349 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23048 0.30200894653021226 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 22194 0.2908185768522879 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 21439 0.28092545143445075 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 18515 0.24261088359106564 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 17600 0.2306212017932895 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 14959 0.19601491804692148 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 14952 0.19592319370529912 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 14839 0.1944425007619672 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14221 0.18634455174445286 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 13099 0.17164245013013063 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 11452 0.15006102289413362 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11151 0.14611687620437336 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 9180 0.12028992229899986 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 8955 0.11734163988971066 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 8061 0.10562713111680151 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3103477374618722E-4 0.0 0.0 2.6206954749237443E-5 1.3103477374618721E-5 2 2.2275911536851826E-4 0.0 0.0 2.6206954749237443E-5 1.3103477374618721E-5 3 2.3586259274313698E-4 0.0 0.0 2.6206954749237443E-5 1.3103477374618721E-5 4 2.6206954749237444E-4 0.0 0.0 3.931043212385616E-5 1.3103477374618721E-5 5 0.0011662094863410662 0.0 0.0 5.2413909498474886E-5 1.3103477374618721E-5 6 0.0022144876763105638 0.0 0.0 6.551738687309361E-5 1.3103477374618721E-5 7 0.002345522450056751 0.0 0.0 6.551738687309361E-5 1.3103477374618721E-5 8 0.0025682815654252695 0.0 0.0 6.551738687309361E-5 1.3103477374618721E-5 9 0.002738626771295313 0.0 0.0 9.172434162233106E-5 1.3103477374618721E-5 10-11 0.004533803171618078 0.0 0.0 1.1793129637156849E-4 1.3103477374618721E-5 12-13 0.008988985478988442 0.0 0.0 1.6379346718273404E-4 2.6206954749237443E-5 14-15 0.014498997715015616 6.551738687309361E-6 0.0 1.9000042193197147E-4 5.2413909498474886E-5 16-17 0.016667623220515015 1.3103477374618721E-5 0.0 2.7517302486699315E-4 6.551738687309361E-5 18-19 0.01773555662654644 1.3103477374618721E-5 0.0 3.7344910517663355E-4 1.1137955768425913E-4 20-21 0.022302118491601065 1.3103477374618721E-5 0.0 8.320708132882888E-4 1.4413825112080593E-4 22-23 0.024274191836481183 1.3103477374618721E-5 0.0 0.002555178088050651 1.506899898081153E-4 24-25 0.03556938933340252 1.3103477374618721E-5 0.0 0.006892429099049448 1.6379346718273404E-4 26-27 0.0713222273500497 1.3103477374618721E-5 0.0 0.014217272951461312 1.703452058700434E-4 28-29 0.07821465644909914 1.3103477374618721E-5 0.0 0.04793252023635528 1.703452058700434E-4 30-31 0.07968224591505645 1.3103477374618721E-5 0.0 0.1443216998040506 1.9000042193197147E-4 32-33 0.08066500671815285 1.3103477374618721E-5 0.0 0.2916506476655762 2.3586259274313698E-4 34-35 0.08137259449638226 1.965521606192808E-5 0.0 0.4841538337760999 2.3586259274313698E-4 36-37 0.08268294223384413 3.931043212385616E-5 0.0 0.7712248160992468 2.3586259274313698E-4 38-39 0.08405880735817911 5.2413909498474886E-5 0.0 1.1692560448306653 2.4241433143044633E-4 40-41 0.08512018902552321 6.551738687309361E-5 0.0 1.683849256548004 2.489660701177557E-4 42-43 0.08780640188732006 7.862086424771233E-5 0.0 2.0780084077152225 2.5551780880506505E-4 44-45 0.09111502992441128 7.862086424771233E-5 0.0 2.4957669216341296 2.6206954749237444E-4 46-47 0.09281848198311171 7.862086424771233E-5 0.0 2.970420734313631 2.6206954749237444E-4 48-49 0.09381434626358273 7.862086424771233E-5 0.0 3.407100669561487 2.7517302486699315E-4 50-51 0.09513124573973192 7.862086424771233E-5 0.0 3.8671310014909137 2.7517302486699315E-4 52-53 0.0957405574376517 7.862086424771233E-5 0.0 4.368515908014637 3.144834569908493E-4 54-55 0.09713607777804859 1.0482781899694977E-4 0.0 4.831566591478913 3.406904117400868E-4 56-57 0.10035953321220478 1.0482781899694977E-4 0.0 5.314619733155546 3.406904117400868E-4 58-59 0.10153229443723316 1.0482781899694977E-4 0.0 5.808922210158288 3.406904117400868E-4 60-61 0.10212850265777831 1.0482781899694977E-4 0.0 6.26935219814764 3.472421504273961E-4 62-63 0.10246264133083109 1.0482781899694977E-4 0.0 6.733601849791694 4.258630146751084E-4 64-65 0.10284919391338235 1.0482781899694977E-4 0.0 7.2152725746053035 4.324147533624178E-4 66-67 0.10340609170180365 1.3103477374618722E-4 0.0 7.7107936750038855 4.324147533624178E-4 68-69 0.10409402426397113 1.3103477374618722E-4 0.0 8.265267320110876 4.324147533624178E-4 70-71 0.10480816378088785 1.3758651243349658E-4 0.0 8.88046247937185 4.5206996942434585E-4 72-73 0.10661644365858522 1.4413825112080593E-4 0.0 9.413164696820914 4.7827692417358334E-4 74-75 0.10826748180778718 1.4413825112080593E-4 0.0 9.928478599793856 5.110356176101301E-4 76-77 0.1087654139480227 1.4413825112080593E-4 0.0 10.472869119061077 5.175873562974395E-4 78-79 0.10926334608825822 1.4413825112080593E-4 0.0 11.016224463615707 5.241390949847489E-4 80-81 0.10981369213799219 1.4413825112080593E-4 0.0 11.598529894666388 5.306908336720581E-4 82-83 0.11020024472054345 1.4413825112080593E-4 0.0 12.219746101780999 5.765530044832237E-4 84-85 0.11144507507113223 1.4413825112080593E-4 0.0 12.80344050143339 5.896564818578425E-4 86-87 0.11379714925987629 1.5724172849542465E-4 0.0 13.386008002031563 6.093116979197706E-4 88 0.11443266791254529 1.5724172849542465E-4 0.0 13.841373496015626 6.158634366070799E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 5715 0.0 22.24655 1 TACACTC 6990 0.0 20.864376 5 TCTACAC 7430 0.0 20.577362 3 AGTACTT 24425 0.0 20.453478 12-13 GAGTACT 23365 0.0 19.219114 12-13 ATCTACA 6810 0.0 19.068148 2 CTACACT 9195 0.0 18.419962 4 CATGGGG 14870 0.0 18.160965 4 TACTTTT 28835 0.0 17.500486 14-15 GTACTTT 26600 0.0 16.974915 14-15 GTACTAG 1795 0.0 16.526894 1 GTATTAG 3435 0.0 16.313057 1 GTATAAG 2430 0.0 16.083736 1 CACTCTT 8900 0.0 15.739166 7 CATGGGT 6820 0.0 15.452602 4 ACTTTTT 30595 0.0 15.029652 16-17 GTATAAA 3910 0.0 14.21086 1 ACTCTTT 12250 0.0 14.1978035 8 ATTGCGT 1175 0.0 14.001951 6 TGCGCGA 1285 0.0 13.929046 94 >>END_MODULE