FastQCFastQC Report
Fri 27 May 2016
ERR765305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7631562
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA469660.6154179183763429No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT421720.5525998478424208No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG258030.3381090266972869No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA230390.3018910152338407No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC217340.2847909772599633No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG212300.2781868246631554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206120.2700888756456411No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG188110.2464895128939528No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT167080.2189328999751296No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC150120.19670940234777626No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT149710.19617215977541685No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC137950.18076247038286525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137830.18060522865436984No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC128710.16865485728871757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115190.15093895587823306No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA97480.1277326974477833No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG87950.11524508350977167No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG82420.1079988605216075No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC77240.10121125924155501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC19450.021.262593
AGTACTT221850.019.12764512-13
GAGTACT210100.018.41919912-13
GATCTAC29000.017.9968811
CATGGGG172950.017.1184544
TCTACAC40650.017.1101683
TATCACG4154.5474735E-1116.993552
TACTTTT257200.016.75455914-15
CATGGGT70300.016.7120324
GTACTTT234000.016.66851214-15
TACACTC37700.016.3295615
GTACTAG19600.016.3126721
CTAACGC8300.015.8537873
CGCTATA2406.7181114E-515.6718292
TCTAACG8150.015.5756842
CGTTAAC7000.015.4490611
TAGTACT21600.015.447184
ATCTACA36500.015.1995292
ACTTTTT267900.014.81402116-17
CGATCTC23500.014.7981855
Produced by FastQC (version 0.11.5)