FastQCFastQC Report
Fri 27 May 2016
ERR765304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13261482
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA211440.15943919390004826No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT198820.1499229120847881No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA165890.1250915998679484No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA146770.11067390507335455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA566700.026.3939061
CGTTAAC13300.021.8921031
CAACGCA657200.021.75075
ATCAACG664350.021.573163
GTACTAG41550.021.1357671
TAGTACT52500.021.1095434
AACGCAG687250.021.1003026
GTACATG983350.020.7744031
TACATGG975800.020.5346812
TATCAAC730400.020.5031172
TCAACGC712250.020.168484
ACATGGG958350.020.0069713
ACGCAGA721700.019.7677487
ATGGGAG144850.019.7111175
CATGGGG375400.019.6270434
CGCAGAG725900.019.5757458
GTATATA29300.019.2336221
CTAGTAC57200.019.1287253
GTATAAG46900.018.9250221
GCAGAGT737800.018.725439
Produced by FastQC (version 0.11.5)