FastQCFastQC Report
Fri 27 May 2016
ERR765303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11658120
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT605450.519337594740833No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA540150.4633251330403187No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG249930.21438276497411246No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG208850.17914552260570316No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG204640.1755343057028063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187720.1610208163923514No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC182700.15671480478842215No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC165430.14190109554542243No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC142660.12236964450528902No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC136400.11699999656891505No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT134880.11569618429043448No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA130300.11176759203027589No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC118880.10197184451695471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG62350.023.6055261
GTGATCG52450.022.131148
CTACACT112850.021.7921644
CTATTGA66250.021.6354689
ATAAGGT61600.021.2980823
TCTACAC82800.020.8426383
GTACTAG62100.020.671731
CGTTAAC22800.020.6239051
TACACTC77850.020.4758195
TAGTACT72750.020.4246334
TGATCGC58050.020.0771399
CATGGGG284250.019.5200864
GCTATTG73100.019.4794928
ATTTAGG75050.019.1724011
TAAGGTG66800.019.1466434
GGGCTAT77250.019.1005656
TAGGCAT95950.018.9655825
CTAGTAC86700.018.6576253
AGGGCTA76250.018.562095
TTTAGGG80600.018.0860462
Produced by FastQC (version 0.11.5)