##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765303_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11658120
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.761530675614935	33.0	33.0	33.0	27.0	33.0
2	31.71078552974236	33.0	33.0	33.0	27.0	33.0
3	31.710793249683483	33.0	33.0	33.0	27.0	33.0
4	35.41713681108103	37.0	37.0	37.0	33.0	37.0
5	35.41192396372657	37.0	37.0	37.0	33.0	37.0
6	35.43131774248335	37.0	37.0	37.0	33.0	37.0
7	35.34271486311687	37.0	37.0	37.0	33.0	37.0
8	35.3391230318439	37.0	37.0	37.0	33.0	37.0
9	35.33193996973783	37.0	37.0	37.0	33.0	37.0
10-11	35.28124547525673	37.0	37.0	37.0	33.0	37.0
12-13	35.28254006649443	37.0	37.0	37.0	33.0	37.0
14-15	36.75101311360665	37.0	37.0	40.0	33.0	40.0
16-17	36.7828816738891	37.0	37.0	40.0	33.0	40.0
18-19	36.8278463422919	37.0	37.0	40.0	33.0	40.0
20-21	36.80203180272634	37.0	37.0	40.0	33.0	40.0
22-23	36.77184644693999	37.0	37.0	40.0	33.0	40.0
24-25	36.73626618185436	37.0	37.0	40.0	33.0	40.0
26-27	36.70905489049693	37.0	37.0	40.0	33.0	40.0
28-29	36.696378361176585	37.0	37.0	40.0	33.0	40.0
30-31	36.625080158721985	37.0	37.0	40.0	33.0	40.0
32-33	36.52917108418853	37.0	37.0	40.0	33.0	40.0
34-35	36.40241115205539	37.0	37.0	40.0	33.0	40.0
36-37	36.33243340264124	37.0	37.0	40.0	33.0	40.0
38-39	36.256875980003635	37.0	37.0	40.0	33.0	40.0
40-41	36.13091960796423	37.0	37.0	40.0	33.0	40.0
42-43	35.995649341403244	37.0	37.0	40.0	27.0	40.0
44-45	35.84610691946901	37.0	37.0	40.0	27.0	40.0
46-47	35.61330420342216	37.0	37.0	40.0	27.0	40.0
48-49	35.40567861713552	37.0	33.0	40.0	27.0	40.0
50-51	35.20855223655272	37.0	33.0	40.0	27.0	40.0
52-53	35.01155426432392	37.0	33.0	40.0	27.0	40.0
54-55	34.88115956946746	37.0	33.0	40.0	27.0	40.0
56-57	34.694892444064735	37.0	33.0	40.0	27.0	40.0
58-59	34.484741450594086	37.0	33.0	38.5	27.0	40.0
60-61	34.26304013854721	37.0	33.0	37.0	27.0	40.0
62-63	34.006046386552896	37.0	33.0	37.0	27.0	40.0
64-65	33.78099685026402	37.0	33.0	37.0	27.0	40.0
66-67	33.56154950369357	37.0	33.0	37.0	27.0	40.0
68-69	33.33801178920787	37.0	33.0	37.0	27.0	40.0
70-71	33.09655493338549	37.0	33.0	37.0	27.0	37.0
72-73	32.85424142142987	37.0	33.0	37.0	22.0	37.0
74-75	32.636681772018136	35.0	33.0	37.0	22.0	37.0
76-77	32.44861071081787	33.0	33.0	37.0	22.0	37.0
78-79	32.235675606358484	33.0	33.0	37.0	22.0	37.0
80-81	32.08711022875043	33.0	33.0	37.0	22.0	37.0
82-83	31.924751031898797	33.0	33.0	37.0	22.0	37.0
84-85	31.768454562142097	33.0	33.0	37.0	22.0	37.0
86-87	31.636314903260562	33.0	33.0	37.0	22.0	37.0
88-89	31.51958501885381	33.0	33.0	37.0	22.0	37.0
90-91	31.404426099576945	33.0	33.0	37.0	22.0	37.0
92-93	31.27339013494457	33.0	33.0	37.0	22.0	37.0
94-95	31.164431100383254	33.0	30.0	37.0	22.0	37.0
96-97	31.053111994043636	33.0	27.0	37.0	22.0	37.0
98-99	30.94096964176042	33.0	27.0	37.0	15.0	37.0
100	30.82520560776523	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	11.0
7	316.0
8	1779.0
9	2742.0
10	3145.0
11	5926.0
12	13098.0
13	22587.0
14	28157.0
15	30267.0
16	33578.0
17	39631.0
18	48729.0
19	59005.0
20	71925.0
21	88832.0
22	107024.0
23	109511.0
24	106331.0
25	107804.0
26	117239.0
27	137889.0
28	169084.0
29	213874.0
30	277585.0
31	367524.0
32	499469.0
33	684614.0
34	965316.0
35	1439157.0
36	2249090.0
37	2807411.0
38	848937.0
39	533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43761387765903	19.94227881798744	12.835188812312111	24.784918492041413
2	15.694590063508024	21.337941282961626	37.66931898325386	25.298149670276494
3	18.037049589648767	26.71025727928338	29.777290609327604	25.475402521740246
4	13.178078472920793	17.853991480914427	36.95405624408611	32.01387380207867
5	13.51566231658346	37.92362456102555	33.904375990012646	14.656337132378344
6	30.584858849802643	37.89192304201677	17.315589116164915	14.20762899201567
7	25.7868764432001	32.825043832110154	22.572198604920864	18.815881119768882
8	26.390426454116394	33.559597743772066	20.215478722756362	19.834497079355177
9	26.780318860211644	14.649935529383024	20.112259161586906	38.45748644881843
10-11	24.806819082970875	26.116609482281707	27.114160659050267	21.96241077569715
12-13	26.06569599054517	24.150501848244158	26.745249049436755	23.038553111773922
14-15	24.00832700241595	24.286987443917607	24.905079129211746	26.799606424454698
16-17	22.999553101186127	26.948933447245356	26.02727112090114	24.02424233066738
18-19	23.412152313701277	26.778846044562464	27.746505011179153	22.062496630557106
20-21	24.60235441048814	25.957658696256342	26.6009270791517	22.83905981410382
22-23	24.6427366717697	25.560840625650698	25.904224122236013	23.892198580343585
24-25	24.129325195663743	26.10414343667714	25.985571308272693	23.780960059386423
26-27	24.240393608781357	25.988622171102023	25.945326509083728	23.825657711032896
28-29	23.59066579764657	25.73485922281894	26.953580557970096	23.720894421564395
30-31	24.279453784008044	25.65213017920084	26.617631812122518	23.4507842246686
32-33	23.720908919324682	26.26521747282547	26.13173307084104	23.88214053700881
34-35	23.622660981074038	26.238993688027794	26.7398313704328	23.398513960465372
36-37	24.232345365501594	25.70876745841541	26.278866706493908	23.780020469589086
38-39	24.385582368518733	25.296083927997454	26.466741657524423	23.851592045959393
40-41	23.74257734825505	25.268475653127513	26.970942147528138	24.0180048510893
42-43	24.261028586408912	25.444444854301423	26.35843925586482	23.93608730342485
44-45	24.162169508925256	25.633715492560633	26.428559450297335	23.77555554821678
46-47	23.782960914498403	25.48305114807069	26.668779565735328	24.065208371695576
48-49	24.573832275921873	25.343439427071424	26.490079832066684	23.592648464940023
50-51	23.728103559170926	25.834837294461327	25.850459188017556	24.5865999583502
52-53	23.336956755335624	26.065894976869853	25.376702696272947	25.220445571521577
54-55	23.533236330278157	26.04778271315641	25.257781801125205	25.161199155440233
56-57	24.28215638046157	25.255023913284557	25.34198560162229	25.120834104631584
58-59	23.42974943229434	25.27183859942117	26.47895785273907	24.81945411554542
60-61	23.896470676644192	25.60351707756271	25.7504537270082	24.749558518784898
62-63	23.50709974222728	25.373362066927907	25.622812759352943	25.496725431491875
64-65	22.678676423028136	25.719129613784865	26.007456585604043	25.59473737758296
66-67	24.040461084653103	25.15495445700195	25.61373228850643	25.190852169838518
68-69	23.537673774885075	24.806547934345318	25.988482646294386	25.667295644475217
70-71	23.211090731768245	25.12872484532988	25.88115923604143	25.779025186860444
72-73	23.932413528515614	24.857657424865646	26.010373198004938	25.199555848613798
74-75	23.785553775760686	25.116810028864588	25.98506370526185	25.11257249011287
76-77	23.286157798161046	25.185493922916212	26.190016311103314	25.33833196781942
78-79	23.39640022915844	25.400979023132574	25.979961374870026	25.22265937283896
80-81	23.016795953465245	25.27762752852128	26.340472969266386	25.365103548747093
82-83	23.20837846136255	24.779290644637342	26.442143704228727	25.57018718977138
84-85	23.4354543590729	25.18387875179821	25.60259525915276	25.77807162997613
86-87	23.020331386276265	24.949309208765598	26.212495934100172	25.817863470857965
88-89	23.362905991439746	24.164981567268534	26.404937148237106	26.06717529305461
90-91	23.39957232176682	24.481171336903422	26.182131363412115	25.937124977917648
92-93	23.226895192236018	24.42790764381557	26.389005046217463	25.956192117730946
94-95	22.314015523739204	24.55701296000966	26.906340667228456	26.222630849022682
96-97	22.989246979787477	24.60049304690636	26.25015868767863	26.16010128562753
98-99	22.87129543477938	24.714523063108796	26.039014416517762	26.375167085594057
100	22.928331289263426	24.458407251807625	26.16996196811937	26.443299490809576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	832.0
1	778.0
2	737.0
3	700.5
4	536.0
5	461.5
6	598.0
7	1089.0
8	1811.0
9	1839.5
10	1321.5
11	1153.0
12	1305.0
13	1549.5
14	1921.5
15	2523.5
16	3196.5
17	3966.0
18	5047.0
19	6084.5
20	7188.0
21	8871.0
22	10836.0
23	12907.0
24	15633.0
25	19444.5
26	24971.5
27	30913.5
28	38557.5
29	53633.5
30	70607.0
31	85694.0
32	106319.5
33	126443.5
34	147181.5
35	165070.0
36	181970.0
37	215155.0
38	239603.0
39	256213.0
40	263814.0
41	264498.0
42	275523.5
43	300676.5
44	341331.5
45	394633.5
46	441568.0
47	485311.0
48	532700.5
49	562610.5
50	625537.5
51	642176.5
52	582060.5
53	547311.0
54	517359.5
55	467107.5
56	427181.5
57	372098.5
58	313693.5
59	282662.5
60	239560.0
61	193996.5
62	155319.5
63	124391.5
64	96587.0
65	69472.0
66	53274.5
67	43507.0
68	35886.0
69	29810.5
70	24670.5
71	20204.0
72	16302.5
73	13333.5
74	11113.0
75	9283.0
76	7624.0
77	5692.0
78	4203.0
79	3026.5
80	2187.5
81	1626.0
82	1074.0
83	686.0
84	438.0
85	291.5
86	191.5
87	96.0
88	52.5
89	30.0
90	18.5
91	14.0
92	11.0
93	7.5
94	5.5
95	5.5
96	7.0
97	5.0
98	3.0
99	3.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03573474968519796
2	0.02487536583943209
3	0.029764661883734257
4	0.028718180976006424
5	0.06334640576696757
6	0.004614809248832574
7	0.0
8	6.690615639571389E-4
9	8.234603864087863E-4
10-11	6.133064336273773E-4
12-13	0.0014024559706024642
14-15	0.003289552689455933
16-17	0.0
18-19	3.045087887240824E-4
20-21	0.0
22-23	1.50109966272435E-4
24-25	0.002032917828946691
26-27	0.009118108237005623
28-29	0.011258247470432624
30-31	0.056715834113905156
32-33	0.040302381516059194
34-35	0.02613200069994133
36-37	0.023854618068779528
38-39	0.0059958209385389755
40-41	0.0098686580683678
42-43	0.00803731647984409
44-45	9.306817908890971E-4
46-47	0.0098686580683678
48-49	0.013625696081357887
50-51	0.012030241582690863
52-53	0.0172540684089716
54-55	0.024163415713682825
56-57	0.025625915670794263
58-59	0.009529838430210016
60-61	0.009053775394317438
62-63	0.019810226691782208
64-65	0.008937976277478702
66-67	0.011910153609672916
68-69	0.004983650880244842
70-71	0.007642741711356548
72-73	0.014873753229508702
74-75	0.003512573210774979
76-77	0.011464112567034822
78-79	0.00586286639698339
80-81	0.0054854470532126965
82-83	7.248166942869004E-4
84-85	0.006090175774481649
86-87	0.001578299073950174
88-89	4.6319646735494226E-4
90-91	4.846407482510045E-4
92-93	6.947947010324135E-4
94-95	8.920820852761852E-4
96-97	0.0
98-99	0.0014839442380075003
100	5.1466274150549143E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.165812E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.28954178635086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.81378271488057	22.157308133068266
2	14.207541003355662	8.890948958115805
3	5.690115952882638	5.34123362630709
4	2.83280972335391	3.5454927284665274
5	1.5863114065631285	2.4817478520911007
6	1.0007176288792747	1.8787197639093614
7	0.6849946946492081	1.5003219085158537
8	0.49792421947316057	1.2463856537313276
9	0.3499960756943766	0.9856095151948215
>10	1.9601018428099002	11.254463582131265
>50	0.17452540556715224	3.8005644233741362
>100	0.1490352398910916	9.750359470506496
>500	0.02541655931919656	5.584873021763456
>1k	0.024504812005696392	14.98406403800468
>5k	0.0017287827473450917	3.5575044628271266
>10k+	4.939379278128833E-4	3.040402861992614
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	60545	0.519337594740833	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	54015	0.4633251330403187	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	24993	0.21438276497411246	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	20885	0.17914552260570316	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	20464	0.1755343057028063	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18772	0.1610208163923514	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18270	0.15671480478842215	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	16543	0.14190109554542243	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	14266	0.12236964450528902	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13640	0.11699999656891505	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	13488	0.11569618429043448	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13030	0.11176759203027589	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	11888	0.10197184451695471	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.688416314122689E-4	0.0	0.0	1.7155424716849713E-5	0.0
2	6.347507145234394E-4	0.0	0.0	1.7155424716849713E-5	0.0
3	6.776392763155637E-4	0.0	0.0	1.7155424716849713E-5	0.0
4	7.719941122582371E-4	0.0	0.0	3.431084943369943E-5	0.0
5	0.0029164222018644515	0.0	0.0	1.0293254830109829E-4	0.0
6	0.005978665513822126	0.0	0.0	1.2008797301794801E-4	0.0
7	0.006982257859757834	0.0	0.0	1.629765348100723E-4	0.0
8	0.009221040785306722	0.0	0.0	1.8013195952692202E-4	0.0
9	0.010704985023314222	3.431084943369943E-5	0.0	2.1444280896062144E-4	0.0
10-11	0.021555791156721668	3.431084943369943E-5	0.0	3.859970561291186E-4	0.0
12-13	0.04049109118794454	3.859970561291186E-5	0.0	9.178152223514597E-4	0.0
14-15	0.06203401577612857	8.577712358424858E-5	0.0	0.0014453445323945884	0.0
16-17	0.07140516652770773	8.577712358424858E-5	0.0	0.0017584310334770958	0.0
18-19	0.07673192590228956	9.435483594267343E-5	0.0	0.0018785190064950438	0.0
20-21	0.10372169783807338	1.0722140448031072E-4	0.0	0.0024146260288965974	0.0
22-23	0.11150597180334393	1.3295454155558528E-4	0.0	0.0031480204355419225	0.0
24-25	0.14883188713102968	1.372433977347977E-4	0.0	0.006724926489005088	0.0
26-27	0.250735109949117	1.372433977347977E-4	0.0	0.01790597454821189	0.0
28-29	0.26909999210850466	1.372433977347977E-4	0.0	0.031548826054286626	0.0
30-31	0.2737448233505917	1.5439882245164743E-4	0.0	0.07562111215187355	0.0
32-33	0.27758334963098685	1.7155424716849715E-4	0.0	0.15720373439285235	0.0
34-35	0.2801609521946935	1.972873842437717E-4	0.0	0.2706654246139172	4.288856179212428E-6
36-37	0.2844069198121138	2.230205213190463E-4	0.0	0.45652300714008776	8.577712358424857E-6
38-39	0.2875377848229389	2.230205213190463E-4	0.0	0.7348740620271537	8.577712358424857E-6
40-41	0.29074584924498975	2.487536583943209E-4	0.0	1.0843129080846654	8.577712358424857E-6
42-43	0.29984251320109934	2.6162022693195815E-4	0.0	1.3692087575012093	8.577712358424857E-6
44-45	0.31199713161298737	2.9164222018644514E-4	0.0	1.672894085838883	8.577712358424857E-6
46-47	0.3173753572617197	3.0879764490329486E-4	0.0	2.0370823082967067	8.577712358424857E-6
48-49	0.32207165477795735	3.0879764490329486E-4	0.0	2.4037023122081433	8.577712358424857E-6
50-51	0.3307179888352496	3.8170819994990616E-4	0.0	2.8010133709380245	8.577712358424857E-6
52-53	0.3335271896326337	3.8599705612911856E-4	0.0	3.202068601112358	1.2866568537637286E-5
54-55	0.3415559284001194	3.9028591230833097E-4	0.0	3.5757866620003913	1.7155424716849713E-5
56-57	0.36068851581558603	3.945747684875434E-4	0.0	3.9909264958672583	1.7155424716849713E-5
58-59	0.36565501127111405	3.945747684875434E-4	0.0	4.43980247243981	1.7155424716849713E-5
60-61	0.3676150185450141	4.074413370251807E-4	0.0	4.885997913900354	1.7155424716849713E-5
62-63	0.3691161182077385	4.20307905562818E-4	0.0	5.344232174655948	1.7155424716849713E-5
64-65	0.37017975454018315	4.460410426380926E-4	0.0	5.816336596295114	1.7155424716849713E-5
66-67	0.3714835668186637	4.460410426380926E-4	0.0	6.294016530967257	1.7155424716849713E-5
68-69	0.372761645960069	4.5461875499651746E-4	0.0	6.828412299753305	1.7155424716849713E-5
70-71	0.3743142118969439	4.760630358925796E-4	0.0	7.446457919458712	1.7155424716849713E-5
72-73	0.3788260885974754	4.8035189207179204E-4	0.0	8.018141003866834	1.7155424716849713E-5
74-75	0.3834880752642793	4.932184606094293E-4	0.0	8.576421412714915	1.7155424716849713E-5
76-77	0.3851178406123801	5.017961729678541E-4	0.0	9.19436409987202	1.7155424716849713E-5
78-79	0.3861514549515702	5.060850291470666E-4	0.0	9.828621595934852	1.7155424716849713E-5
80-81	0.3882186836299506	5.060850291470666E-4	0.0	10.525354859960268	1.7155424716849713E-5
82-83	0.3888834563377286	5.060850291470666E-4	0.0	11.281111362724008	2.144428089606214E-5
84-85	0.3914867920385105	5.146627415054915E-4	0.0	12.001562001420469	3.0021993254487E-5
86-87	0.3966033974603109	5.232404538639163E-4	0.0	12.732554648605436	3.431084943369943E-5
88	0.39767132264893484	5.40395878580766E-4	0.0	13.304100489615822	3.431084943369943E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAG	6235	0.0	23.605526	1
GTGATCG	5245	0.0	22.13114	8
CTACACT	11285	0.0	21.792164	4
CTATTGA	6625	0.0	21.635468	9
ATAAGGT	6160	0.0	21.298082	3
TCTACAC	8280	0.0	20.842638	3
GTACTAG	6210	0.0	20.67173	1
CGTTAAC	2280	0.0	20.623905	1
TACACTC	7785	0.0	20.475819	5
TAGTACT	7275	0.0	20.424633	4
TGATCGC	5805	0.0	20.077139	9
CATGGGG	28425	0.0	19.520086	4
GCTATTG	7310	0.0	19.479492	8
ATTTAGG	7505	0.0	19.172401	1
TAAGGTG	6680	0.0	19.146643	4
GGGCTAT	7725	0.0	19.100565	6
TAGGCAT	9595	0.0	18.965582	5
CTAGTAC	8670	0.0	18.657625	3
AGGGCTA	7625	0.0	18.56209	5
TTTAGGG	8060	0.0	18.086046	2
>>END_MODULE