FastQCFastQC Report
Fri 27 May 2016
ERR765302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11753928
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213830.1819221625315384No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT164480.13993619835003243No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC154390.13135183404220274No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA150650.1281699190262183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145160.12349914003216628No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC142940.12161040972856053No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT126280.1074364246573571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA464250.025.793861
GTACTAG73900.024.1566981
GTACAAG51750.023.1487871
ACGTTAA16350.022.1198758
TAGTACT86150.021.8649584
CAACGCA508850.021.406275
GTATAAG75200.021.3651851
ATCAACG511500.021.3069083
GTGATCG58500.021.2764788
TATCAAC563950.021.1746752
TAGGCAT113150.020.3409655
AACGCAG559650.020.2765376
CTGTGCG95100.019.9530229
GTACATA24900.019.6215081
CGCAGAG546550.019.4904948
CGTTAAC22450.019.4609931
GTACATG691950.019.3562341
ATAAGGT67750.019.3451883
TACATGA36800.019.2757322
ACGCAGA556250.019.2521887
Produced by FastQC (version 0.11.5)