FastQCFastQC Report
Fri 27 May 2016
ERR765301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12979210
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA424850.3273311703871037No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT423840.32655300284069677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209680.16155066448574296No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC169880.1308862403798074No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG165180.12726506466880497No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC145640.1122102192660416No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT139410.10741023529167029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131690.10146226157061948No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA130660.10066868476586788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA21800.028.0117841
GTACTAG82250.026.0996191
TAGGCAT107350.024.6757725
GTGATCG59850.023.777468
GTACAAG43350.023.4055351
TACATGA39050.022.6142582
GTATAAG77700.022.1265621
ATAAGGT66500.022.1088713
CTAGGCA120900.022.0672134
TAAGGTG69900.021.704554
TGATCGC66450.021.4158139
ATGGGTA65850.021.3259835
CATGGGT94600.021.3005894
TAGTACT90250.021.0261424
ACGTTAA19600.020.8473198
TCTAACG39800.020.7718492
TATTCGC13400.020.679229
CTAACGC41200.020.6359863
CATGGGG366900.020.4191864
GTACAAA45400.020.382821
Produced by FastQC (version 0.11.5)