##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765301_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12979210
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77508299811776	33.0	33.0	33.0	27.0	33.0
2	31.689768868829457	33.0	33.0	33.0	27.0	33.0
3	31.693831057514288	33.0	33.0	33.0	27.0	33.0
4	35.430889784509226	37.0	37.0	37.0	33.0	37.0
5	35.51443886030044	37.0	37.0	37.0	33.0	37.0
6	35.499194326927444	37.0	37.0	37.0	33.0	37.0
7	35.3145406384518	37.0	37.0	37.0	33.0	37.0
8	35.28334197535906	37.0	37.0	37.0	33.0	37.0
9	35.11668106148217	37.0	37.0	37.0	33.0	37.0
10-11	35.110300896587695	37.0	37.0	37.0	33.0	37.0
12-13	35.06112502224711	37.0	37.0	37.0	33.0	37.0
14-15	36.64372049608566	40.0	37.0	40.0	33.0	40.0
16-17	36.70925171871015	40.0	37.0	40.0	33.0	40.0
18-19	36.67039203464618	40.0	37.0	40.0	33.0	40.0
20-21	36.39090888428495	40.0	37.0	40.0	33.0	40.0
22-23	36.44395321440982	38.5	37.0	40.0	33.0	40.0
24-25	36.33899759692616	37.0	37.0	40.0	33.0	40.0
26-27	36.3043798505456	37.0	37.0	40.0	30.0	40.0
28-29	36.235750943239225	37.0	37.0	40.0	30.0	40.0
30-31	36.329710436921815	37.0	37.0	40.0	33.0	40.0
32-33	36.085364170854774	37.0	37.0	40.0	27.0	40.0
34-35	35.890521187344994	37.0	37.0	40.0	27.0	40.0
36-37	35.96935187118476	37.0	37.0	40.0	27.0	40.0
38-39	35.86744867368661	37.0	37.0	40.0	27.0	40.0
40-41	35.871427074529194	37.0	37.0	40.0	27.0	40.0
42-43	35.72045178404541	37.0	37.0	40.0	27.0	40.0
44-45	35.60978815351628	37.0	37.0	40.0	27.0	40.0
46-47	35.38196954976459	37.0	33.0	40.0	27.0	40.0
48-49	35.09839886248855	37.0	33.0	40.0	27.0	40.0
50-51	34.88836315923697	37.0	33.0	40.0	27.0	40.0
52-53	34.73059978226718	37.0	33.0	40.0	27.0	40.0
54-55	34.581033244704415	37.0	33.0	40.0	27.0	40.0
56-57	34.40386849430743	37.0	33.0	40.0	27.0	40.0
58-59	34.21699494807465	37.0	33.0	37.0	27.0	40.0
60-61	33.99016326879679	37.0	33.0	37.0	27.0	40.0
62-63	33.75126987698019	37.0	33.0	37.0	27.0	40.0
64-65	33.56275925114086	37.0	33.0	37.0	27.0	40.0
66-67	33.287741280093314	37.0	33.0	37.0	22.0	40.0
68-69	33.09542591575296	37.0	33.0	37.0	22.0	38.5
70-71	32.84336770111587	37.0	33.0	37.0	22.0	37.0
72-73	32.633011023012955	37.0	33.0	37.0	22.0	37.0
74-75	32.4473204455433	37.0	33.0	37.0	22.0	37.0
76-77	32.19691861060881	33.0	33.0	37.0	22.0	37.0
78-79	32.13456084769412	33.0	33.0	37.0	22.0	37.0
80-81	32.00645012292736	33.0	33.0	37.0	22.0	37.0
82-83	31.868493074694065	33.0	33.0	37.0	22.0	37.0
84-85	31.717810945350294	33.0	33.0	37.0	22.0	37.0
86-87	31.558478944404165	33.0	33.0	37.0	22.0	37.0
88-89	31.436958643861992	33.0	33.0	37.0	22.0	37.0
90-91	31.31718837279002	33.0	33.0	37.0	18.5	37.0
92-93	31.190635215856744	33.0	33.0	37.0	15.0	37.0
94-95	31.057866734570133	33.0	27.0	37.0	15.0	37.0
96-97	30.93338893507386	33.0	27.0	37.0	15.0	37.0
98-99	30.814609017035707	33.0	27.0	37.0	15.0	37.0
100	30.60149007528193	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	7.0
6	23.0
7	350.0
8	1740.0
9	3365.0
10	5180.0
11	8861.0
12	16939.0
13	29970.0
14	45041.0
15	53030.0
16	59167.0
17	67309.0
18	76046.0
19	85839.0
20	96716.0
21	111497.0
22	127246.0
23	127216.0
24	122477.0
25	128662.0
26	144503.0
27	169606.0
28	208162.0
29	259098.0
30	322676.0
31	407763.0
32	533102.0
33	711630.0
34	959054.0
35	1378199.0
36	2292356.0
37	3316778.0
38	1108773.0
39	829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77290643358809	19.92115843285047	12.872863195255018	24.433071938306426
2	15.889549044067733	21.46979257121239	37.52957021766115	25.111088167058725
3	17.402494230598823	27.708104901005164	29.623962182008718	25.26543868638729
4	12.615733062167433	17.87690014349632	37.531519607015944	31.9758471873203
5	13.548312819434354	38.12128231256005	33.27566307757881	15.05474179042679
6	29.957307719660214	38.301329725574064	17.637800662978933	14.103561891786793
7	26.163874805680493	32.79956800419727	23.043711600415907	17.99284558970633
8	26.885136007181288	32.34396978003456	20.33355999591622	20.437334216867935
9	27.156627848322067	14.28072931036834	20.50587714724731	38.05676569406228
10-11	25.430359262189313	25.29944551094077	26.39815331597833	22.872041910891586
12-13	26.319528687885164	23.768243292959404	26.29426111316784	23.617966905987593
14-15	24.49563247062946	24.30669376566608	24.38818646349043	26.80948730021403
16-17	23.440163367970836	26.418225657383765	25.586014870818484	24.55559610382691
18-19	23.7784860808255	25.563738760378406	27.989696389120954	22.66807876967514
20-21	25.453167494475316	24.831766832456985	26.53473481184017	23.18033086122753
22-23	24.753006556747962	24.518161089950507	26.35224739490825	24.376584958393277
24-25	24.043950217599082	25.468739193015562	26.491702451532213	23.995608137853147
26-27	24.30934091550412	25.2317803038579	26.330121522890842	24.128757257747143
28-29	23.384502694114012	25.147992840358867	27.402043568253664	24.065460897273454
30-31	24.244467508268446	25.132890170844256	26.846670084620566	23.775972236266732
32-33	23.691479950029656	25.36675125441294	26.115585765935943	24.82618302962146
34-35	24.24689669094833	25.427931425216865	27.02726149902691	23.297910384807892
36-37	24.36727008361941	25.239332678782482	26.226670064288115	24.166727173309987
38-39	24.45489327377606	24.690853326871792	26.441516358703527	24.412737040648622
40-41	23.97713661148272	25.082209382452646	27.017442371342376	23.923211634722257
42-43	24.789429991255872	25.063208677882088	26.284391970849253	23.862969360012787
44-45	24.09194737286531	25.580500237656185	25.972643543430806	24.3549088460477
46-47	23.715622530977203	25.34358776713622	26.450249551962692	24.49054014992388
48-49	24.873112763371374	25.5343162103012	25.71445276535705	23.87811826097038
50-51	23.667395174044916	26.031671089035985	25.351840928059687	24.94909280885941
52-53	23.566868131952827	25.727109335380355	25.214810241897073	25.491212290769745
54-55	23.96846865402395	25.670403903754686	25.29394168066655	25.06718576155481
56-57	24.431208991504363	24.93184371938439	24.848265242812985	25.788682046298263
58-59	23.56137807468344	25.07827745480992	26.125452382443175	25.23489208806346
60-61	23.864605474082246	25.53318799003129	25.507375753227773	25.094830782658693
62-63	23.4649138523822	25.427644007897726	25.20583345644154	25.901608683278532
64-65	23.148648291437883	25.250022907832832	25.58403028504126	26.01729851568803
66-67	24.38109267215851	25.10721361285927	25.264083387671953	25.247610327310273
68-69	24.141357889775307	24.67448914131473	25.423178759154485	25.760974209755478
70-71	23.273585809026923	25.011077545904044	25.599806867866526	26.115529777202507
72-73	24.384307711277916	25.087420428798225	25.50848502597142	25.019786833952434
74-75	24.693357417956776	24.978115191981175	25.3758081696733	24.95271922038875
76-77	23.90925040429816	25.39052934476937	25.445920571947273	25.2542996789852
78-79	24.080733575885738	25.54641723997352	25.30033392699846	25.072515257142285
80-81	23.55346806921951	25.3572137844998	25.84827990452875	25.24103824175194
82-83	24.027608188419848	25.208831247859475	25.455918361181613	25.307642202539064
84-85	24.08136367135934	25.562229439689933	24.70493315193672	25.651473737014012
86-87	23.60403586612771	25.231126524833925	25.166079891831455	25.99875771720691
88-89	23.983552928448233	24.47683559841573	25.3816341625204	26.15797731061564
90-91	24.25516310806574	24.86521338503763	25.02096286767097	25.858660639225654
92-93	23.989005635101414	24.96293548278824	25.04897394880721	25.999084933303134
94-95	22.964945594403076	25.18272158193814	25.777907494589613	26.07442532906917
96-97	24.03841062675304	25.10048785540693	24.88928474566108	25.971816772178947
98-99	23.424152832422536	25.327332590690823	24.9478480984561	26.30066647843054
100	23.43866352525206	25.419829682544933	24.62544242141434	26.516064370788666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1208.0
1	1046.5
2	1180.5
3	1474.0
4	1222.0
5	1051.0
6	1338.0
7	2444.0
8	4057.0
9	4135.0
10	2736.5
11	1923.0
12	1847.0
13	1897.0
14	2219.5
15	2783.5
16	3383.5
17	4198.0
18	5278.0
19	6532.0
20	8191.5
21	10060.0
22	12391.5
23	15591.5
24	19035.0
25	23577.5
26	30178.5
27	36698.0
28	44063.0
29	58427.0
30	77281.5
31	94574.0
32	115897.0
33	137891.5
34	160042.0
35	178227.0
36	192632.0
37	219315.0
38	236480.5
39	246163.5
40	245348.5
41	239723.0
42	250610.5
43	289369.5
44	348842.0
45	414841.0
46	493735.5
47	562015.0
48	623923.5
49	661498.0
50	694601.0
51	682120.0
52	615012.0
53	589575.0
54	566479.5
55	524778.0
56	492303.5
57	437444.0
58	398748.0
59	375703.5
60	317984.5
61	251207.0
62	204334.5
63	162041.5
64	117852.5
65	90889.0
66	68011.5
67	45858.0
68	34618.5
69	29705.5
70	28461.0
71	27795.0
72	25589.5
73	28494.0
74	22972.5
75	15795.5
76	11243.5
77	7222.5
78	5102.0
79	2630.5
80	1493.5
81	1139.5
82	981.0
83	830.0
84	666.0
85	504.0
86	358.0
87	218.0
88	123.0
89	90.5
90	73.0
91	52.0
92	37.0
93	28.0
94	21.0
95	15.0
96	11.0
97	7.0
98	3.5
99	3.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017712942467222582
2	0.006009610754429584
3	0.009707832757155482
4	0.009068348535850795
5	0.019731555310377135
6	0.0039832932821026856
7	0.024331218926267467
8	0.007974291193377718
9	0.0016102674970202345
10-11	4.160499753066635E-4
12-13	9.630786465432026E-4
14-15	0.0010247156799219675
16-17	0.001282820757195546
18-19	0.0035364247901066397
20-21	0.006691470436182171
22-23	0.013406054759881379
24-25	0.02263234819376526
26-27	0.027016282192829918
28-29	0.03280631101584765
30-31	0.035333429384377016
32-33	0.03632347423302343
34-35	0.04499888668108459
36-37	0.04182072714749203
38-39	0.046532107886381374
40-41	0.043377062240305846
42-43	0.050122465080694435
44-45	0.04342714232992609
46-47	0.0459195898671799
48-49	0.04833884342729642
50-51	0.04882808737974037
52-53	0.032898766565915796
54-55	0.029312261686188914
56-57	0.02144198298663786
58-59	0.021361084380328233
60-61	0.021623041772187983
62-63	0.021734758895186994
64-65	0.0254175716395682
66-67	0.02680440489059041
68-69	0.027782892795478308
70-71	0.028025588614407196
72-73	0.026272785477698568
74-75	0.021129945505157864
76-77	0.025644858200152394
78-79	0.025579368852187458
80-81	0.024323514297095124
82-83	0.02636524102776671
84-85	0.027667323357893122
86-87	0.03007501997425113
88-89	0.02997871210959681
90-91	0.02994018896373508
92-93	0.032355590209265435
94-95	0.030329272736938535
96-97	0.03281401564502
98-99	0.03368078642690888
100	0.04189006881004314
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.297921E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.20146196439422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.74309900520139	14.786556608377577
2	13.228384761731371	5.609221927524395
3	5.772149829357981	3.6713404517975343
4	3.081847859485526	2.6135872069172854
5	1.8461303729935437	1.9570331442167768
6	1.1864014287195366	1.509206685930013
7	0.8293903058789089	1.2309000916610284
8	0.5923017068099087	1.0046129686700844
9	0.45619853942036553	0.8704868383559768
>10	2.6208262651388234	10.505427858715615
>50	0.2747297300695909	4.083741116286889
>100	0.25605912524664065	11.71068364854566
>500	0.05234203141174999	7.804259883505775
>1k	0.05466832600741241	23.149430046499482
>5k	0.004345530518270729	6.035319012919842
>10k+	0.0011251820091950995	3.458192510076463
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	42485	0.3273311703871037	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	42384	0.32655300284069677	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20968	0.16155066448574296	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	16988	0.1308862403798074	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	16518	0.12726506466880497	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	14564	0.1122102192660416	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13941	0.10741023529167029	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13169	0.10146226157061948	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	13066	0.10066868476586788	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5409258344691242E-5	0.0
5	0.0	0.0	0.0	3.0818516689382484E-5	0.0
6	0.0	0.0	0.0	3.0818516689382484E-5	0.0
7	0.0	0.0	0.0	4.622777503407372E-5	0.0
8	0.0	0.0	0.0	6.934166255111059E-5	0.0
9	0.0	0.0	0.0	7.70462917234562E-5	0.0
10-11	0.0	0.0	0.0	1.0786480841283869E-4	0.0
12-13	7.704629172345621E-6	0.0	0.0	1.3097869592987554E-4	0.0
14-15	1.926157293086405E-5	0.0	0.0	1.3868332510222114E-4	0.0
16-17	3.0818516689382484E-5	0.0	0.0	1.540925834469124E-4	0.0
18-19	3.0818516689382484E-5	0.0	0.0	1.7335415637777647E-4	0.0
20-21	3.85231458617281E-5	0.0	3.8523145861728105E-6	3.390036835832073E-4	7.704629172345621E-6
22-23	3.85231458617281E-5	0.0	7.704629172345621E-6	0.0018452586867767763	7.704629172345621E-6
24-25	3.85231458617281E-5	0.0	7.704629172345621E-6	0.00559356077912292	7.704629172345621E-6
26-27	6.934166255111059E-5	0.0	7.704629172345621E-6	0.009388090646503137	7.704629172345621E-6
28-29	7.70462917234562E-5	7.704629172345621E-6	7.704629172345621E-6	0.02389590737802994	7.704629172345621E-6
30-31	8.0898606309629E-5	7.704629172345621E-6	7.704629172345621E-6	0.06960747225755651	7.704629172345621E-6
32-33	8.475092089580182E-5	7.704629172345621E-6	7.704629172345621E-6	0.1551712315310408	7.704629172345621E-6
34-35	1.0016017924049307E-4	7.704629172345621E-6	7.704629172345621E-6	0.268375347960315	7.704629172345621E-6
36-37	1.155694375851843E-4	7.704629172345621E-6	7.704629172345621E-6	0.4375690045850248	7.704629172345621E-6
38-39	1.2327406675752994E-4	7.704629172345621E-6	7.704629172345621E-6	0.6928079598064905	7.704629172345621E-6
40-41	1.2327406675752994E-4	7.704629172345621E-6	7.704629172345621E-6	1.010211715505027	7.704629172345621E-6
42-43	1.2712638134370274E-4	7.704629172345621E-6	7.704629172345621E-6	1.286503569939927	7.704629172345621E-6
44-45	1.3483101051604834E-4	1.5409258344691242E-5	7.704629172345621E-6	1.6092890091153467	7.704629172345621E-6
46-47	1.502402688607396E-4	1.5409258344691242E-5	7.704629172345621E-6	1.960889761395339	7.704629172345621E-6
48-49	1.540925834469124E-4	1.5409258344691242E-5	7.704629172345621E-6	2.3317752004937127	7.704629172345621E-6
50-51	1.540925834469124E-4	1.5409258344691242E-5	7.704629172345621E-6	2.7345616566801834	7.704629172345621E-6
52-53	1.6179721261925804E-4	1.5409258344691242E-5	7.704629172345621E-6	3.139601716899565	7.704629172345621E-6
54-55	1.6179721261925804E-4	1.5409258344691242E-5	7.704629172345621E-6	3.543204863778304	7.704629172345621E-6
56-57	1.6179721261925804E-4	1.5409258344691242E-5	7.704629172345621E-6	3.9944611420880007	7.704629172345621E-6
58-59	1.6179721261925804E-4	1.5409258344691242E-5	7.704629172345621E-6	4.486733013796679	7.704629172345621E-6
60-61	1.6179721261925804E-4	1.5409258344691242E-5	7.704629172345621E-6	4.9708649447847755	7.704629172345621E-6
62-63	1.6950184179160367E-4	1.5409258344691242E-5	7.704629172345621E-6	5.455000728087457	7.704629172345621E-6
64-65	1.7720647096394927E-4	1.5409258344691242E-5	7.704629172345621E-6	5.966911699556444	7.704629172345621E-6
66-67	1.7720647096394927E-4	1.5409258344691242E-5	7.704629172345621E-6	6.512561242171134	7.704629172345621E-6
68-69	1.8491110013629488E-4	1.5409258344691242E-5	7.704629172345621E-6	7.090342940749091	7.704629172345621E-6
70-71	1.926157293086405E-4	1.5409258344691242E-5	7.704629172345621E-6	7.747732720250308	7.704629172345621E-6
72-73	1.926157293086405E-4	1.5409258344691242E-5	7.704629172345621E-6	8.370424702273867	7.704629172345621E-6
74-75	1.926157293086405E-4	1.5409258344691242E-5	7.704629172345621E-6	8.982411102062452	7.704629172345621E-6
76-77	1.926157293086405E-4	1.5409258344691242E-5	7.704629172345621E-6	9.655418164896016	1.5409258344691242E-5
78-79	1.926157293086405E-4	2.311388751703686E-5	7.704629172345621E-6	10.334434838484007	1.5409258344691242E-5
80-81	1.926157293086405E-4	2.311388751703686E-5	7.704629172345621E-6	11.060430488450375	1.5409258344691242E-5
82-83	1.926157293086405E-4	3.467083127555529E-5	7.704629172345621E-6	11.826717496673528	1.5409258344691242E-5
84-85	1.926157293086405E-4	3.85231458617281E-5	7.704629172345621E-6	12.561850836838296	1.5409258344691242E-5
86-87	1.926157293086405E-4	4.622777503407372E-5	7.704629172345621E-6	13.321454079254439	1.5409258344691242E-5
88	1.926157293086405E-4	4.622777503407372E-5	7.704629172345621E-6	13.904929498790759	1.5409258344691242E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	2180	0.0	28.011784	1
GTACTAG	8225	0.0	26.099619	1
TAGGCAT	10735	0.0	24.675772	5
GTGATCG	5985	0.0	23.77746	8
GTACAAG	4335	0.0	23.405535	1
TACATGA	3905	0.0	22.614258	2
GTATAAG	7770	0.0	22.126562	1
ATAAGGT	6650	0.0	22.108871	3
CTAGGCA	12090	0.0	22.067213	4
TAAGGTG	6990	0.0	21.70455	4
TGATCGC	6645	0.0	21.415813	9
ATGGGTA	6585	0.0	21.325983	5
CATGGGT	9460	0.0	21.300589	4
TAGTACT	9025	0.0	21.026142	4
ACGTTAA	1960	0.0	20.847319	8
TCTAACG	3980	0.0	20.771849	2
TATTCGC	1340	0.0	20.67922	9
CTAACGC	4120	0.0	20.635986	3
CATGGGG	36690	0.0	20.419186	4
GTACAAA	4540	0.0	20.38282	1
>>END_MODULE