##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765297_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6100651 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4381220954944 34.0 31.0 34.0 31.0 34.0 2 32.457148261718295 34.0 31.0 34.0 31.0 34.0 3 32.492150919631364 34.0 31.0 34.0 31.0 34.0 4 35.937955473932206 37.0 35.0 37.0 35.0 37.0 5 35.95325367735345 37.0 37.0 37.0 35.0 37.0 6 36.073695085983445 37.0 37.0 37.0 35.0 37.0 7 36.029752234638565 37.0 37.0 37.0 35.0 37.0 8 36.07065049287363 37.0 37.0 37.0 35.0 37.0 9 37.79438194382862 39.0 38.0 39.0 35.0 39.0 10-11 37.73978260680704 39.0 38.0 39.0 35.0 39.0 12-13 37.71797009860095 39.0 38.0 39.0 35.0 39.0 14-15 39.081065446949836 41.0 39.0 41.0 36.0 41.0 16-17 39.03182447250302 41.0 39.0 41.0 36.0 41.0 18-19 39.05392883480796 41.0 39.0 41.0 36.0 41.0 20-21 39.05005318284885 41.0 39.0 41.0 36.0 41.0 22-23 38.98206994630573 41.0 39.0 41.0 35.5 41.0 24-25 38.918992825519766 41.0 39.0 41.0 35.0 41.0 26-27 38.765666565748475 41.0 39.0 41.0 35.0 41.0 28-29 38.59052746993723 40.0 38.0 41.0 35.0 41.0 30-31 38.30135882219783 40.0 38.0 41.0 34.5 41.0 32-33 37.931775641648734 40.0 37.5 41.0 34.0 41.0 34-35 37.81945139953097 40.0 38.0 41.0 34.0 41.0 36-37 37.66908211926891 40.0 37.5 41.0 33.5 41.0 38-39 37.43179686889153 40.0 37.0 41.0 33.0 41.0 40-41 37.1288350210494 40.0 37.0 41.0 32.5 41.0 42-43 36.79419679965302 40.0 36.5 41.0 31.5 41.0 44-45 36.27479698478081 40.0 36.0 41.0 30.5 41.0 46-47 35.763640224625206 39.0 35.0 41.0 28.5 41.0 48-49 35.33445102825912 39.0 35.0 41.0 26.5 41.0 50-51 34.08797774204753 38.0 34.0 40.0 23.5 40.5 52-53 34.00940219330691 38.0 34.5 39.5 23.0 40.5 54-55 34.753809306580564 39.0 35.0 40.5 23.0 41.0 56-57 34.71437728530938 39.0 35.0 41.0 22.0 41.0 58-59 34.49515182887859 39.0 35.0 41.0 20.0 41.0 60-61 34.06602787145175 38.0 35.0 40.5 16.5 41.0 62-63 33.61125984751463 37.5 34.0 40.0 9.5 41.0 64-65 33.33793582029196 37.0 34.0 40.0 4.5 41.0 66-67 32.944640580161035 37.0 34.0 39.0 2.0 41.0 68-69 32.631477116130725 36.0 34.0 39.0 2.0 41.0 70-71 32.17528358858751 35.0 34.0 39.0 2.0 40.5 72-73 31.676292251433495 35.0 33.5 37.5 2.0 39.5 74-75 31.201525296234777 35.0 33.0 37.0 2.0 39.0 76-77 30.75267909932891 35.0 33.0 36.5 2.0 38.5 78-79 30.42062494641965 35.0 32.5 36.0 2.0 37.0 80-81 30.097694164114614 35.0 32.0 35.5 2.0 37.0 82-83 29.82318682055407 35.0 32.0 35.0 2.0 36.5 84-85 29.53365649010245 35.0 31.5 35.0 2.0 36.0 86-87 29.22537340687084 35.0 31.0 35.0 2.0 36.0 88-89 28.944247179522314 35.0 31.0 35.0 2.0 35.5 90-91 28.683048087818825 35.0 31.0 35.0 2.0 35.0 92-93 28.351518551052994 35.0 30.0 35.0 2.0 35.0 94-95 28.010033273498188 35.0 29.0 35.0 2.0 35.0 96-97 27.664060114240268 35.0 29.0 35.0 2.0 35.0 98-99 27.284415630397476 35.0 28.0 35.0 2.0 35.0 100 26.69157603016465 34.0 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40094066736729417 1101 2 0.47257042238213387 1101 3 0.4423412785882661 1101 4 0.40415781577100773 1101 5 0.4360573823957168 1101 6 0.37158770663941 1101 7 0.35486014766516405 1101 8 0.3001266620935752 1101 9 0.4251330208907902 1101 10-11 0.38527470956876897 1101 12-13 0.42723121198987 1101 14-15 0.5801196946248126 1101 16-17 0.5991994761456425 1101 18-19 0.5606945487718775 1101 20-21 0.5921410558720197 1101 22-23 0.6497509353200925 1101 24-25 0.5831611682157956 1101 26-27 0.6213709569492067 1101 28-29 0.6092961748850456 1101 30-31 0.6405687809768352 1101 32-33 0.6319630380379095 1101 34-35 0.709752779931577 1101 36-37 0.7111175085528529 1101 38-39 0.7244536824154295 1101 40-41 0.7666126631659793 1101 42-43 0.7037135604243545 1101 44-45 0.7456876896165525 1101 46-47 0.8437809764646858 1101 48-49 0.7899488771547638 1101 50-51 0.7790070591763012 1101 52-53 0.8566048630404808 1101 54-55 1.0023335975782501 1101 56-57 1.0526361738109387 1101 58-59 1.009666453238843 1101 60-61 1.0315814138831314 1101 62-63 1.0642629208634204 1101 64-65 0.979232680298864 1101 66-67 0.9391057406787482 1101 68-69 0.9202188336912016 1101 70-71 0.8563735651253594 1101 72-73 0.840982699990068 1101 74-75 0.7960101561183528 1101 76-77 0.8794829724067306 1101 78-79 0.8904362996604007 1101 80-81 0.8759990055498932 1101 82-83 0.8564942399464108 1101 84-85 0.8228974912787947 1101 86-87 0.8349556725490253 1101 88-89 0.901505304008154 1101 90-91 0.9585253576755122 1101 92-93 0.9570483323476466 1101 94-95 0.8675691480693608 1101 96-97 0.9248442284882543 1101 98-99 1.0174564107894177 1101 100 1.0399476167834472 1102 1 0.44901038926840897 1102 2 0.5513926243387814 1102 3 0.5785465872288853 1102 4 0.4547743774686239 1102 5 0.4240747127597828 1102 6 0.37197042348056897 1102 7 0.4163273922655435 1102 8 0.36483596327730794 1102 9 0.37518569225260023 1102 10-11 0.4447702759106207 1102 12-13 0.47899883543124844 1102 14-15 0.6714246443423875 1102 16-17 0.6362852942272212 1102 18-19 0.6116703202930509 1102 20-21 0.5961259081410475 1102 22-23 0.5821015623448034 1102 24-25 0.6030217737739463 1102 26-27 0.5517861873414418 1102 28-29 0.58562921396787 1102 30-31 0.506106945684472 1102 32-33 0.6182126928193199 1102 34-35 0.6448741065581487 1102 36-37 0.6476481265084644 1102 38-39 0.6880806787956075 1102 40-41 0.6729625299711302 1102 42-43 0.7004957495264534 1102 44-45 0.6541379801389553 1102 46-47 0.5927348924557378 1102 48-49 0.54149108524323 1102 50-51 0.5293524950172639 1102 52-53 0.6242905263320466 1102 54-55 0.8377304212370333 1102 56-57 0.9021239554628124 1102 58-59 0.8991679584700876 1102 60-61 0.9110017542925561 1102 62-63 0.8487575356272856 1102 64-65 0.9105762858078279 1102 66-67 0.8650227881069625 1102 68-69 0.8930581477138446 1102 70-71 0.8476286504189048 1102 72-73 0.8019463208598516 1102 74-75 0.8195780051923514 1102 76-77 0.7820837957867361 1102 78-79 0.7875238801165381 1102 80-81 0.8764017967757702 1102 82-83 0.8717533359455949 1102 84-85 0.7904321708646762 1102 86-87 0.7681705843929301 1102 88-89 0.7378250279514731 1102 90-91 0.8143718093151016 1102 92-93 0.8065130414924049 1102 94-95 0.7688515362641617 1102 96-97 0.827247697202889 1102 98-99 0.8373975224514929 1102 100 0.8348517124967252 1103 1 0.40562931822238113 1103 2 0.5048298035367864 1103 3 0.542528472353375 1103 4 0.41243461527755443 1103 5 0.43070128322045065 1103 6 0.39107640159588186 1103 7 0.4170868744908134 1103 8 0.41889884509669173 1103 9 0.49409875713234896 1103 10-11 0.473947276629886 1103 12-13 0.509520552953667 1103 14-15 0.6603821757475075 1103 16-17 0.6298283848718711 1103 18-19 0.6227975580136871 1103 20-21 0.6411000244822986 1103 22-23 0.6374679639912415 1103 24-25 0.6236893466102842 1103 26-27 0.6243110066767272 1103 28-29 0.667784911673472 1103 30-31 0.7114379981959544 1103 32-33 0.7425036830324601 1103 34-35 0.7206875175088783 1103 36-37 0.683093824553147 1103 38-39 0.6579196190947201 1103 40-41 0.6280617983030083 1103 42-43 0.6140903748854143 1103 44-45 0.6371892738959986 1103 46-47 0.6775434542848586 1103 48-49 0.7311676554008528 1103 50-51 0.7279749107260329 1103 52-53 0.708639860636417 1103 54-55 0.7201958170056528 1103 56-57 0.6968316428513077 1103 58-59 0.6955392092381274 1103 60-61 0.6924208779124825 1103 62-63 0.7014136430912785 1103 64-65 0.713405551615466 1103 66-67 0.6813194928239099 1103 68-69 0.6506859357376271 1103 70-71 0.6695210183637599 1103 72-73 0.6523380688844007 1103 74-75 0.6760411473307606 1103 76-77 0.7116236575057684 1103 78-79 0.7236739727401158 1103 80-81 0.725986354347274 1103 82-83 0.6280845249067077 1103 84-85 0.6682713427841627 1103 86-87 0.6721510804503872 1103 88-89 0.7406426799793735 1103 90-91 0.7549844435676931 1103 92-93 0.733864139813619 1103 94-95 0.673086503503967 1103 96-97 0.7548808678614023 1103 98-99 0.7955848440370552 1103 100 0.7247610250936454 1104 1 0.3965333076000661 1104 2 0.4454360826069177 1104 3 0.49806395847355844 1104 4 0.41718894028193176 1104 5 0.4038493010321744 1104 6 0.3589068525731065 1104 7 0.3797721880646989 1104 8 0.35772809072660294 1104 9 0.45463077619201897 1104 10-11 0.44228423434893926 1104 12-13 0.4541302181849787 1104 14-15 0.545706953404391 1104 16-17 0.5636970046530649 1104 18-19 0.6041105843824255 1104 20-21 0.5930587308378392 1104 22-23 0.5688379026276209 1104 24-25 0.59319525448592 1104 26-27 0.5427506417114003 1104 28-29 0.5823978686708386 1104 30-31 0.5812530991086362 1104 32-33 0.6221049269671823 1104 34-35 0.5920350312717559 1104 36-37 0.5406906377978942 1104 38-39 0.5987614712989497 1104 40-41 0.6562539537515661 1104 42-43 0.7058039833976224 1104 44-45 0.7431356992348483 1104 46-47 0.6945874664636449 1104 48-49 0.6899622047911791 1104 50-51 0.69561161420404 1104 52-53 0.7028326379467842 1104 54-55 0.7751836342292506 1104 56-57 0.7911081882943805 1104 58-59 0.7745698866731701 1104 60-61 0.7614579428514219 1104 62-63 0.7500624922511108 1104 64-65 0.773104963462977 1104 66-67 0.7545221761183925 1104 68-69 0.7648946109359969 1104 70-71 0.7301898274177248 1104 72-73 0.721333704014679 1104 74-75 0.6842367852959086 1104 76-77 0.6844547127776544 1104 78-79 0.7366573547500188 1104 80-81 0.7050192898765388 1104 82-83 0.7751647186157555 1104 84-85 0.7083292367007807 1104 86-87 0.6700232319060042 1104 88-89 0.6231612572337539 1104 90-91 0.5865304456967131 1104 92-93 0.6827102762898143 1104 94-95 0.713635787371441 1104 96-97 0.6777656687913591 1104 98-99 0.709093822920817 1104 100 0.7484940476368145 1105 1 0.27117658915607024 1105 2 0.2410246853165816 1105 3 0.28085302814465507 1105 4 0.28617651549576806 1105 5 0.2672232801101302 1105 6 0.3036129934603409 1105 7 0.23492968482077004 1105 8 0.25411375474156017 1105 9 0.2661640009919566 1105 10-11 0.3171922838269339 1105 12-13 0.3140472210059855 1105 14-15 0.4214166759344735 1105 16-17 0.39655526241188 1105 18-19 0.3811429511359634 1105 20-21 0.37085942207687594 1105 22-23 0.4186801404753737 1105 24-25 0.4368843421998818 1105 26-27 0.3255958639841836 1105 28-29 0.4458987726649042 1105 30-31 0.45361465272122814 1105 32-33 0.4483126417403511 1105 34-35 0.45782594941111654 1105 36-37 0.43106255683776595 1105 38-39 0.4245277081030423 1105 40-41 0.41891441273991603 1105 42-43 0.41005089618463586 1105 44-45 0.44953060610048823 1105 46-47 0.42799486508425844 1105 48-49 0.5309570713193139 1105 50-51 0.46804649730729864 1105 52-53 0.5172002701676561 1105 54-55 0.6133423783643437 1105 56-57 0.5670570322724302 1105 58-59 0.5665515973955522 1105 60-61 0.5467932197292527 1105 62-63 0.5928278042947 1105 64-65 0.5511880477829223 1105 66-67 0.4995390869319962 1105 68-69 0.4480492005936014 1105 70-71 0.46403023102639196 1105 72-73 0.469916449364451 1105 74-75 0.45303594450451357 1105 76-77 0.47554653975374706 1105 78-79 0.48937717316809426 1105 80-81 0.464191240892994 1105 82-83 0.4467188633586545 1105 84-85 0.44197710497516596 1105 86-87 0.5066108408487615 1105 88-89 0.5169646656678069 1105 90-91 0.5657719987713783 1105 92-93 0.6009579802732681 1105 94-95 0.6078827792848891 1105 96-97 0.6389833064610855 1105 98-99 0.6268875686926059 1105 100 0.7367893120418749 1106 1 0.2688340018499389 1106 2 0.29378556358850716 1106 3 0.32153711601996804 1106 4 0.34261227395344207 1106 5 0.3079745998087873 1106 6 0.305888098311911 1106 7 0.3046436030738917 1106 8 0.29779479669572595 1106 9 0.29971759947974874 1106 10-11 0.30451661014276254 1106 12-13 0.3882351414085221 1106 14-15 0.5071550455182674 1106 16-17 0.47752048582704987 1106 18-19 0.45232708775905195 1106 20-21 0.49784755418760085 1106 22-23 0.48988468787800343 1106 24-25 0.49097683978003914 1106 26-27 0.4489129536996401 1106 28-29 0.4572221969589876 1106 30-31 0.5006778532668008 1106 32-33 0.4959666583812634 1106 34-35 0.46604347337020613 1106 36-37 0.45924235578641515 1106 38-39 0.49726049500185354 1106 40-41 0.408424979477239 1106 42-43 0.3546182108416005 1106 44-45 0.3380269247038896 1106 46-47 0.296785774364281 1106 48-49 0.34216367327132957 1106 50-51 0.34864368758099573 1106 52-53 0.3846979850375547 1106 54-55 0.34414398032387794 1106 56-57 0.35551225675528997 1106 58-59 0.32309463854468135 1106 60-61 0.3464344599427349 1106 62-63 0.31340181995314254 1106 64-65 0.3182261457326092 1106 66-67 0.26968173807389206 1106 68-69 0.24820721104292431 1106 70-71 0.28569786659156904 1106 72-73 0.15659751187036974 1106 74-75 0.29917980155850543 1106 76-77 0.25476791503739804 1106 78-79 0.19307496731264706 1106 80-81 0.22123523118253985 1106 82-83 0.2121220155725041 1106 84-85 0.11827816607253894 1106 86-87 0.16661166229256708 1106 88-89 0.17195748383034726 1106 90-91 0.19132511053335222 1106 92-93 0.2532234295572877 1106 94-95 0.22688357654278235 1106 96-97 0.14945702827948537 1106 98-99 0.05096119186255876 1106 100 0.07451659695894008 1107 1 0.22678217404271095 1107 2 0.2410184873312815 1107 3 0.29540465802733706 1107 4 0.27091895020467405 1107 5 0.3189884300421113 1107 6 0.27619933472129077 1107 7 0.26012636956983215 1107 8 0.3063184174163638 1107 9 0.34476975301256374 1107 10-11 0.3237257615340212 1107 12-13 0.3258259327344817 1107 14-15 0.3607383545157816 1107 16-17 0.3925381667357897 1107 18-19 0.3706483561419489 1107 20-21 0.384311363080279 1107 22-23 0.3408189025423951 1107 24-25 0.3816217296323785 1107 26-27 0.357482578478745 1107 28-29 0.3889929834926704 1107 30-31 0.2838719108341152 1107 32-33 0.2519630978637011 1107 34-35 0.31360226309374895 1107 36-37 0.23879633637766773 1107 38-39 0.24526963778197342 1107 40-41 0.22627277577520033 1107 42-43 0.19430342701854642 1107 44-45 0.21041269611192348 1107 46-47 0.1442977644352652 1107 48-49 0.1647223225959138 1107 50-51 0.18461581932389493 1107 52-53 0.2178731355452328 1107 54-55 0.2585406298431536 1107 56-57 0.20492314272887313 1107 58-59 0.15072262623450428 1107 60-61 0.2200931819003813 1107 62-63 0.17292353686391237 1107 64-65 0.2681945091674791 1107 66-67 0.2499741703828775 1107 68-69 0.25198604898882593 1107 70-71 0.18722601564658703 1107 72-73 0.23631136007475817 1107 74-75 0.17742137949302972 1107 76-77 0.18650406982818524 1107 78-79 0.18857939268779234 1107 80-81 0.21800020519937036 1107 82-83 0.2107180259957211 1107 84-85 0.21037918031460734 1107 86-87 0.12001465222877172 1107 88-89 0.10470006514942654 1107 90-91 0.05393303053215348 1107 92-93 0.03847058788238655 1107 94-95 -0.06483269775559464 1107 96-97 -0.07878862122480967 1107 98-99 -0.13116152805836734 1107 100 -0.046415109244072994 1108 1 0.12286166266486731 1108 2 0.12802190374389966 1108 3 0.21883473429640787 1108 4 0.16643072708506423 1108 5 0.19043617894742226 1108 6 0.17627491877688328 1108 7 0.197616476804086 1108 8 0.22199589064105396 1108 9 0.21377939239049226 1108 10-11 0.22383705099202444 1108 12-13 0.24418983221999468 1108 14-15 0.3072927707129196 1108 16-17 0.2960561423068029 1108 18-19 0.2825318705710984 1108 20-21 0.30621284077682986 1108 22-23 0.2751669343641865 1108 24-25 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2340.0 4 2185.0 5 2398.0 6 2918.0 7 3520.0 8 4657.0 9 5835.0 10 8446.0 11 14354.0 12 25632.0 13 39501.0 14 39910.0 15 46959.0 16 62675.0 17 86854.0 18 86883.0 19 49462.0 20 41634.0 21 37040.0 22 37375.0 23 42856.0 24 46830.0 25 46914.0 26 48675.0 27 54224.0 28 62359.0 29 71878.0 30 86224.0 31 107108.0 32 136610.0 33 182865.0 34 262986.0 35 416930.0 36 766589.0 37 1548872.0 38 1345342.0 39 256035.0 40 330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.30230946518477 16.917889036471596 13.765687589577743 23.014113908765886 2 13.193936937283457 22.689671247134534 37.06570947939545 27.050682336186547 3 19.371370239290922 29.328104888885964 29.28469159465364 22.01583327716947 4 12.852236377343901 19.031819925897405 33.49647323866605 34.61947045809264 5 12.28015391445908 36.79647443803134 33.333481328005526 17.589890319504054 6 29.478739035554007 32.84917890721603 19.062057660142116 18.610024397087848 7 23.346303693637847 33.37209388252778 22.03896034583978 21.24264207799459 8 28.072023290257825 30.3548533573319 21.787895671885 19.78522768052527 9 28.360559345918723 14.945241387918943 19.088320024176568 37.60587924198576 10-11 23.709352785557236 27.091467814069265 25.27220803593606 23.926971364437442 12-13 27.55331464851946 24.64884642798345 26.050807183302098 21.74703174019499 14-15 26.671426467480742 22.263964203145477 26.021826124919567 25.042783204454217 16-17 22.016143589341155 26.375017781950316 28.936989469227353 22.671849159481173 18-19 24.16315336285406 25.202719655104016 30.647776815938567 19.986350166103357 20-21 23.434163787284586 24.666076404632424 30.046689064430844 21.853070743652143 22-23 21.684004491628766 27.01155794793842 26.21443997240922 25.089997588023593 24-25 21.8893669211954 27.912491867460904 25.36212432144672 24.836016889896978 26-27 23.39711844507511 24.84872665989286 26.26110111061606 25.49305378441597 28-29 22.689030281659424 23.780458605734548 30.444128869316987 23.086382243289037 30-31 25.114214718247357 24.097732073083616 28.89669757145517 21.89135563721386 32-33 19.84617151257013 26.234707792735833 29.81463961325864 24.104481081435395 34-35 19.283397974450644 27.085853888201783 30.101893071455 23.52885506589257 36-37 22.45074373066025 27.037333819006403 27.749378907808484 22.76254354252486 38-39 23.939512167104613 24.493281066988306 27.295429976841167 24.271776789065914 40-41 21.890562896860153 26.497818256254078 27.254596388687997 24.357022458197775 42-43 24.958623230563585 25.20195227783526 26.945151266248125 22.894273225353025 44-45 24.585144656804005 24.30029796101829 28.417005255002586 22.697552127175115 46-47 22.36654417048184 25.108600053378225 30.499201379621837 22.025654396518096 48-49 22.905519852077965 26.41455371474256 29.422211381833396 21.257715051346075 50-51 21.60417173810704 27.096759304429984 27.215780055696325 24.083288901766654 52-53 20.676732888440668 28.147553221663703 24.728286204650626 26.447427685245007 54-55 21.969996805029268 26.9705894536436 24.533060564587007 26.52635317674012 56-57 23.147553381060337 24.48231375423295 25.553348565449134 26.816784299257574 58-59 23.026994029076807 24.049776542809944 28.519355333003453 24.403874095109796 60-61 23.13865153666227 25.23705181538555 27.040916885356015 24.58337976259617 62-63 19.653578162238627 25.8629051035212 28.312843792064168 26.170672942176004 64-65 19.89135404246129 25.967271648370836 27.78690189830938 26.35447241085849 66-67 22.636248170279554 25.56068279804719 25.911631364348043 25.891437667325214 68-69 23.593405867640683 23.94876736390998 26.00585049367386 26.45197627477548 70-71 22.1371930956455 25.02561391539091 26.38899226547973 26.44820072348386 72-73 24.307672279360425 23.94813909709325 26.31015250918061 25.434036114365714 74-75 24.44178123529658 23.052234755454172 27.128389946371783 25.377594062877463 76-77 22.370783961670103 24.73698685693691 28.641204804044474 24.251024377348514 78-79 22.728865082510357 24.445021647296794 28.43673521876654 24.38937805142631 80-81 21.972937996527662 24.93581454205294 27.371334143578853 25.719913317840547 82-83 22.074086026473942 25.66706223577829 25.357610590757666 26.901241146990106 84-85 22.72483626899625 25.35848085673351 25.327628256876544 26.589054617393693 86-87 23.232917816224223 23.34135892216093 26.28407300097274 27.141650260642102 88-89 23.461372820124577 22.38576264874905 28.01776459011947 26.1350999410069 90-91 24.07887039123225 23.31717819142866 26.953661391575256 25.650290025763834 92-93 22.638678752781964 23.885652259467122 27.523368803597016 25.952300184153902 94-95 22.323907691826843 23.986841553318524 27.812027830303908 25.877222924550725 96-97 24.236480000342166 23.590556059159066 26.124045921292705 26.048918019206063 98-99 25.091622641360384 22.641500273487523 25.761323523276392 26.5055535618757 100 24.648729968643927 22.95899871289616 26.537749942295996 25.85452137616392 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1714.0 1 2574.0 2 2164.0 3 780.5 4 578.0 5 607.0 6 805.5 7 1197.0 8 1847.0 9 2038.5 10 1710.0 11 1622.0 12 1804.5 13 2038.0 14 2381.5 15 2717.5 16 3159.5 17 3829.0 18 4532.5 19 5268.0 20 6236.5 21 7581.0 22 9289.5 23 11224.5 24 13717.5 25 16985.0 26 21153.0 27 25954.0 28 31213.0 29 38242.5 30 46014.0 31 54099.0 32 63092.5 33 71250.5 34 79056.5 35 85174.5 36 89813.0 37 95925.0 38 99220.0 39 98612.5 40 97072.5 41 96315.0 42 98491.0 43 106995.5 44 120028.5 45 132964.0 46 157094.0 47 198105.0 48 339410.5 49 500640.0 50 657618.5 51 623887.0 52 396467.5 53 290655.5 54 228364.5 55 172037.0 56 142556.0 57 122858.0 58 110574.0 59 99122.5 60 82852.5 61 67950.5 62 55334.5 63 42673.5 64 31226.5 65 25004.0 66 20000.5 67 15005.5 68 11442.5 69 9234.5 70 7874.5 71 6559.5 72 5388.5 73 5346.0 74 4442.0 75 3484.5 76 2700.0 77 1805.5 78 1319.5 79 892.5 80 620.0 81 477.0 82 362.0 83 277.0 84 199.5 85 129.0 86 90.5 87 65.0 88 50.5 89 42.0 90 36.5 91 33.0 92 26.5 93 21.0 94 20.5 95 16.5 96 10.5 97 6.5 98 4.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08830205169907276 2 0.27854404390613396 3 0.28293701770515967 4 0.2906738969332945 5 0.31719565666024824 6 0.16670352065705776 7 0.18237397943268677 8 0.1504265692300707 9 0.19851979731343425 10-11 0.2195667314848858 12-13 0.23645017556323086 14-15 0.16490043439626362 16-17 0.15576206539269336 18-19 0.17157185356120191 20-21 0.20316684235829915 22-23 0.16735918838825561 24-25 0.19154513182281693 26-27 0.18863560626562642 28-29 0.21887008452048806 30-31 0.25246485989773876 32-33 0.22099280880024116 34-35 0.14865626635583645 36-37 0.14361582067225284 38-39 0.11250438682691405 40-41 0.10373483092214258 42-43 0.11893812643929312 44-45 0.13237111908220942 46-47 0.19712650338463877 48-49 0.20175715673622371 50-51 0.20714182797868624 52-53 0.1376082650851524 54-55 0.08414675745260629 56-57 0.12105265487240624 58-59 0.14005882323050442 60-61 0.11723339033817867 62-63 0.1376328526250723 64-65 0.11331577564427141 66-67 0.11745467819745793 68-69 0.10316112165734442 70-71 0.12738804432510564 72-73 0.1521149136379052 74-75 0.18860282287906652 76-77 0.1699162925399273 78-79 0.1950611500313655 80-81 0.10166128172222931 82-83 0.1407144909617023 84-85 0.17051458934464533 86-87 0.1831034097836444 88-89 0.16568723567370106 90-91 0.16932619158184922 92-93 0.1209379130194466 94-95 0.3551424266033248 96-97 0.3554456729290038 98-99 0.40168663967173346 100 0.43518306488930447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6100651.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.52806585345059 #Duplication Level Percentage of deduplicated Percentage of total 1 80.90995789020357 27.127543963426596 2 10.655246407725917 7.144996064859548 3 3.099808814274316 3.1179178217428754 4 1.4806664576618085 1.9857552999792207 5 0.7834294724010578 1.3133437471098357 6 0.5309299685854901 1.068063297018286 7 0.3461870228714176 0.8124886910310026 8 0.26984589429452094 0.723792873135197 9 0.21744818931764995 0.6561555490040182 >10 1.3739563014032607 8.959418563978947 >50 0.16316192930989293 3.826585328283452 >100 0.14129626861082178 9.54930736718512 >500 0.015805242300753742 3.659991802717303 >1k 0.009010465101425074 6.024339339047092 >5k 0.0013294128838168137 3.174430597392467 >10k+ 0.0019202630544020642 20.85586969408906 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 145221 2.380418089807137 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 122882 2.014244053626408 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 85183 1.396293608665698 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 61450 1.0072695520527235 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 59142 0.9694375239626065 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 59059 0.9680770134203711 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 55845 0.9153941112186225 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 55182 0.9045264185740177 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 49713 0.8148802480259892 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 45750 0.7499199675575606 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 40437 0.6628309011612039 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 38487 0.6308670992653079 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 25858 0.4238564048328613 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25741 0.42193857671910756 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 25158 0.4123822195368986 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 23050 0.377828530102771 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 22760 0.37307493905158645 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 19687 0.3227032656023103 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 19476 0.3192446183202416 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 18553 0.30411508542285076 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 17749 0.2909361640257736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17488 0.2866579320797076 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 17120 0.2806257889526872 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 16494 0.27036458895944054 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 16332 0.2677091346480892 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 15351 0.2516288835404615 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 15054 0.24676055063631736 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 14921 0.24458045543008441 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 14765 0.24202335127841276 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 14246 0.2335160624661204 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 13276 0.21761611998457214 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 12224 0.20037205865406824 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 12178 0.19961804076319067 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 12150 0.19915907335135216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11934 0.19561846760288368 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 11928 0.19552011744320402 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 10750 0.17621070275942682 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 10440 0.17112927784264334 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 10316 0.16909670787592998 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 9678 0.158638807563324 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 9551 0.15655706251677076 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 9274 0.1520165634782255 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 9166 0.1502462606039913 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 9065 0.14859069958271665 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 8940 0.14654173792272332 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 8591 0.14082103696802195 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 8465 0.13875568361474866 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8158 0.13372343377780502 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 7719 0.12652748042790843 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 7644 0.12529810343191244 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA 7506 0.12303604975927979 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT 7086 0.11615153858170219 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 6891 0.11295515839211258 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT 6562 0.10756229130301012 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 6163 0.1010220056843114 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.786587857590936E-4 0.0 0.0 0.0 0.0 2 5.409258782382405E-4 0.0 0.0 0.0 0.0 3 5.573175715181871E-4 0.0 0.0 0.0 0.0 4 6.228843446379738E-4 3.278338655989336E-5 0.0 1.639169327994668E-5 0.0 5 0.0028521546307107223 3.278338655989336E-5 0.0 4.9175079839840044E-5 0.0 6 0.005228950156302991 3.278338655989336E-5 0.0 4.9175079839840044E-5 0.0 7 0.0060485348203003254 3.278338655989336E-5 0.0 4.9175079839840044E-5 0.0 8 0.006868119484297659 3.278338655989336E-5 0.0 4.9175079839840044E-5 0.0 9 0.007474612135655687 3.278338655989336E-5 0.0 1.3113354623957345E-4 0.0 10-11 0.014023093600994387 3.278338655989336E-5 0.0 2.622670924791469E-4 0.0 12-13 0.026488976340393836 3.278338655989336E-5 0.0 3.196380189589603E-4 0.0 14-15 0.040921862273386886 3.278338655989336E-5 0.0 4.671632584784804E-4 0.0 16-17 0.04629014182256942 3.278338655989336E-5 0.0 0.0010162849833566944 0.0 18-19 0.04909312137344031 4.09792331998667E-5 0.0 0.001491644088475148 6.556677311978673E-5 20-21 0.05891994149476834 4.9175079839840044E-5 0.0 0.0029505047903904025 7.376261975976006E-5 22-23 0.06284575203531557 6.556677311978673E-5 0.0 0.008031929707173873 8.19584663997334E-5 24-25 0.08692514946355726 1.1474185295962676E-4 0.0 0.019366785610257002 8.19584663997334E-5 26-27 0.16505615548242308 1.1474185295962676E-4 0.0 0.03865161275411427 8.19584663997334E-5 28-29 0.1817510950880488 1.1474185295962676E-4 0.0 0.11215196542139519 9.835015967968009E-5 30-31 0.18557035962227636 1.1474185295962676E-4 0.0 0.2876250419832244 9.835015967968009E-5 32-33 0.18829957655338753 1.1474185295962676E-4 0.0 0.5454991606633456 9.835015967968009E-5 34-35 0.19036492990666076 1.229376995996001E-4 0.0 0.8664567109313416 1.1474185295962676E-4 36-37 0.19473331616576656 1.3113354623957345E-4 0.0 1.31884285791795 1.3113354623957345E-4 38-39 0.1994213404438313 1.3113354623957345E-4 0.0 1.9894270300005688 1.4752523951952012E-4 40-41 0.20386348932269688 1.803086260794135E-4 0.0 3.038257720364597 1.5572108615949346E-4 42-43 0.21432958548194284 1.8850447271938684E-4 0.0 3.7360193199053677 1.639169327994668E-4 44-45 0.22560707045854617 2.048961659993335E-4 0.0 4.389556130976842 1.7211277943944016E-4 46-47 0.2299918484109319 2.1309201263930685E-4 0.0 5.1339193145125 1.9670031935936018E-4 48-49 0.23299972412780212 2.1309201263930685E-4 0.0 5.775555756262733 2.1309201263930685E-4 50-51 0.23712223498770868 2.1309201263930685E-4 0.0 6.5075841906052325 2.212878592792802E-4 52-53 0.23895810463506273 2.1309201263930685E-4 0.0 7.464711552914599 2.458753991992002E-4 54-55 0.24470339312968403 2.1309201263930685E-4 0.0 8.209820558494496 2.622670924791469E-4 56-57 0.2570955132493237 2.1309201263930685E-4 0.0 8.932825365686384 2.704629391191202E-4 58-59 0.26136554934874984 2.1309201263930685E-4 0.0 9.676778756890045 2.8685463239906694E-4 60-61 0.26440620845218 2.1309201263930685E-4 0.0 10.28085363348928 2.9505047903904025E-4 62-63 0.26649614934537313 2.1309201263930685E-4 0.0 10.898050060559111 2.9505047903904025E-4 64-65 0.26824186467968747 2.1309201263930685E-4 0.0 11.576936625288024 2.9505047903904025E-4 66-67 0.2707497937515193 2.1309201263930685E-4 0.0 12.228662154252063 2.9505047903904025E-4 68-69 0.27338066052295074 2.1309201263930685E-4 0.0 13.036059594295757 2.9505047903904025E-4 70-71 0.27660982409910023 2.212878592792802E-4 0.0 14.057663682121794 3.0324632567901356E-4 72-73 0.28504335029163286 2.2948370591925352E-4 0.0 14.810673483862622 3.60617252158827E-4 74-75 0.2935178557173652 2.2948370591925352E-4 0.0 15.50967265624603 3.60617252158827E-4 76-77 0.29580449692991784 2.3767955255922686E-4 0.0 16.24443850336628 3.60617252158827E-4 78-79 0.297574799804152 2.540712458391736E-4 0.0 16.913645773213382 3.60617252158827E-4 80-81 0.3008203550735815 2.622670924791469E-4 0.0 17.678785427981374 3.9340063871872035E-4 82-83 0.3023693700885365 2.786587857590936E-4 0.0 18.65185371200549 4.5896741183850705E-4 84-85 0.30822940043611735 3.114421723189869E-4 0.0 19.483281374397585 4.5896741183850705E-4 86-87 0.32017894483719855 3.278338655989336E-4 0.0 20.251978026607325 4.5896741183850705E-4 88 0.32306388285446913 3.60617252158827E-4 0.0 20.8622325715731 4.5896741183850705E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 10340 0.0 33.283142 1 ATCTACA 11735 0.0 29.50098 2 TATCACG 370 0.0 26.696804 2 TCTACAC 14220 0.0 26.061993 3 TACACTC 15075 0.0 24.648878 5 CTACACT 15780 0.0 23.93573 4 AGTACTC 3640 0.0 23.2594 5 GTATATA 1035 0.0 21.833752 1 ACACTCT 17475 0.0 21.250874 6 GATGTAA 2395 0.0 21.229792 1 CACTCTT 17540 0.0 21.118692 7 AAAGTAC 4320 0.0 20.793644 3 AAGTACT 4360 0.0 20.71347 4 GTAAGAA 2450 0.0 20.350632 4 ACTCTTT 19145 0.0 20.035734 8 TATACCG 590 0.0 19.930372 5 TGTAAGA 2680 0.0 18.95265 3 AAAAGTA 4730 0.0 18.397223 2 TCACGCA 615 0.0 18.355839 4 GTATAAG 1335 0.0 18.337898 1 >>END_MODULE