##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765297_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6100651 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.551777179189564 34.0 31.0 34.0 31.0 34.0 2 32.66185461190945 34.0 31.0 34.0 31.0 34.0 3 32.710537613117026 34.0 31.0 34.0 31.0 34.0 4 36.20107575404658 37.0 35.0 37.0 35.0 37.0 5 36.13551242318238 37.0 35.0 37.0 35.0 37.0 6 36.232771551757345 37.0 37.0 37.0 35.0 37.0 7 36.16500271856233 37.0 37.0 37.0 35.0 37.0 8 36.19185214823795 37.0 37.0 37.0 35.0 37.0 9 37.930750341234074 39.0 38.0 39.0 35.0 39.0 10-11 37.87528183467633 39.0 38.0 39.0 35.0 39.0 12-13 37.86649834583227 39.0 38.0 39.0 35.0 39.0 14-15 39.163431492802985 40.5 38.5 41.0 36.0 41.0 16-17 39.1274412353698 40.0 38.5 41.0 36.0 41.0 18-19 39.18063080481083 40.0 39.0 41.0 36.0 41.0 20-21 39.20680251992779 40.0 39.0 41.0 36.0 41.0 22-23 39.163824073857036 40.0 39.0 41.0 35.5 41.0 24-25 39.117657525401796 40.0 39.0 41.0 35.0 41.0 26-27 38.93980134251247 40.0 38.0 41.0 35.0 41.0 28-29 38.77884106138836 40.0 38.0 41.0 35.0 41.0 30-31 38.52915680638017 40.0 38.0 41.0 34.5 41.0 32-33 38.144523428729165 40.0 37.0 41.0 34.0 41.0 34-35 38.11915949625704 40.0 38.0 41.0 34.0 41.0 36-37 38.016189009992544 40.0 38.0 41.0 34.0 41.0 38-39 37.78787575293194 40.0 37.5 41.0 33.0 41.0 40-41 37.495046594207736 40.0 37.0 41.0 33.0 41.0 42-43 37.173322978154296 40.0 37.0 41.0 32.5 41.0 44-45 36.697372870534636 40.0 36.0 41.0 31.5 41.0 46-47 36.28100345356586 39.0 36.0 41.0 30.5 41.0 48-49 35.958179135308676 39.0 35.5 41.0 30.0 41.0 50-51 35.68647296821274 39.0 35.0 41.0 28.5 41.0 52-53 35.537803916336145 39.0 35.0 41.0 28.0 41.0 54-55 35.49481842183728 39.0 35.0 41.0 28.0 41.0 56-57 35.29393272947428 39.0 35.0 41.0 27.0 41.0 58-59 35.17074907251701 39.0 35.0 41.0 27.0 41.0 60-61 34.78633821210228 38.5 35.0 41.0 26.0 41.0 62-63 34.37300183209956 38.0 35.0 40.0 26.0 41.0 64-65 34.18087036940811 37.0 35.0 40.0 26.0 41.0 66-67 33.74930650843656 37.0 35.0 39.0 24.5 41.0 68-69 33.369651697827 36.0 34.0 39.0 23.0 41.0 70-71 32.93050143337162 35.0 34.0 38.5 22.5 40.5 72-73 32.466605285239226 35.0 34.0 37.0 20.5 39.0 74-75 32.07453073450686 35.0 33.5 37.0 19.5 39.0 76-77 30.6444208986877 34.0 31.0 35.5 16.5 37.0 78-79 31.36395042102884 35.0 33.0 36.0 16.0 37.0 80-81 31.299644005205344 35.0 33.0 35.0 13.0 37.0 82-83 31.130609913597745 35.0 33.0 35.0 7.5 36.5 84-85 30.933702813027658 35.0 33.0 35.0 3.5 36.0 86-87 30.741647899543835 35.0 33.0 35.0 2.0 36.0 88-89 30.573273327715356 35.0 33.0 35.0 2.0 35.5 90-91 30.440307845834816 35.0 33.0 35.0 2.0 35.0 92-93 30.275587720064628 35.0 33.0 35.0 2.0 35.0 94-95 30.19900794193931 35.0 33.0 35.0 2.0 35.0 96-97 30.098306967567886 35.0 33.0 35.0 2.0 35.0 98-99 29.958666296432956 35.0 33.0 35.0 2.0 35.0 100 29.625454889978133 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.401735604189156 1101 2 0.33406552103291887 1101 3 0.36276581895232596 1101 4 0.24808965558474227 1101 5 0.22439785239447474 1101 6 0.23783862926032384 1101 7 0.23839643554762802 1101 8 0.26995219123732284 1101 9 0.2957494303221395 1101 10-11 0.3206592152764003 1101 12-13 0.36414584669305583 1101 14-15 0.5105535682422371 1101 16-17 0.48704061476640703 1101 18-19 0.4502756566434414 1101 20-21 0.44719455505175887 1101 22-23 0.4519089840588535 1101 24-25 0.42144866257611824 1101 26-27 0.481550164784899 1101 28-29 0.514949175013399 1101 30-31 0.5064989823421158 1101 32-33 0.5548278552494494 1101 34-35 0.5644624595280447 1101 36-37 0.6004495326823474 1101 38-39 0.6601945114403307 1101 40-41 0.6622961967660359 1101 42-43 0.6728584840864258 1101 44-45 0.7542668758125686 1101 46-47 0.7856599613718416 1101 48-49 0.7893841959559325 1101 50-51 0.8142783841279169 1101 52-53 0.8906779122000259 1101 54-55 0.9820188028496446 1101 56-57 0.9767569054073988 1101 58-59 0.9359540165828051 1101 60-61 1.000063047587581 1101 62-63 0.9367326322829967 1101 64-65 0.9801567301413954 1101 66-67 1.0375481021548225 1101 68-69 0.9712522684824947 1101 70-71 0.9450089699640358 1101 72-73 1.0008403332065754 1101 74-75 0.9585601272854873 1101 76-77 0.9720227087030295 1101 78-79 1.0243035689986293 1101 80-81 0.9844062461528722 1101 82-83 0.973778446844527 1101 84-85 1.003895926875817 1101 86-87 1.0546308958421164 1101 88-89 1.006855679786348 1101 90-91 1.0934234293994152 1101 92-93 1.172511002591932 1101 94-95 1.1837507983400464 1101 96-97 1.1926456938153862 1101 98-99 1.1691091921667152 1101 100 1.2143190665128145 1102 1 0.3461803962419978 1102 2 0.2760497670729265 1102 3 0.341538156484944 1102 4 0.21593266381558607 1102 5 0.24737372437447647 1102 6 0.2405872557292028 1102 7 0.22057976734279805 1102 8 0.24886852554691785 1102 9 0.2748393081221465 1102 10-11 0.3064919572409508 1102 12-13 0.30854857813029213 1102 14-15 0.41560702954412676 1102 16-17 0.43951637733666615 1102 18-19 0.3523189596114449 1102 20-21 0.37494619267834395 1102 22-23 0.40428055101318705 1102 24-25 0.3818945207438986 1102 26-27 0.39678598840215074 1102 28-29 0.4532633716356571 1102 30-31 0.5037460976704153 1102 32-33 0.4988920595625359 1102 34-35 0.511959818138763 1102 36-37 0.5575447066438315 1102 38-39 0.664376457668439 1102 40-41 0.6405876180863572 1102 42-43 0.6738764118155132 1102 44-45 0.6870766500739407 1102 46-47 0.6993700013972131 1102 48-49 0.6249616021750981 1102 50-51 0.6564801200494728 1102 52-53 0.6710256022814178 1102 54-55 0.767579358751945 1102 56-57 0.8630405997407422 1102 58-59 0.8238191987530641 1102 60-61 0.7878379124070278 1102 62-63 0.7174624743419074 1102 64-65 0.7561126488782008 1102 66-67 0.7575089145225888 1102 68-69 0.7549141004378228 1102 70-71 0.7219922018565583 1102 72-73 0.744429478508799 1102 74-75 0.7018373375056655 1102 76-77 0.6864316501642165 1102 78-79 0.7472545956687107 1102 80-81 0.8432317017240933 1102 82-83 0.8559505421983786 1102 84-85 0.8367742408221588 1102 86-87 0.8787464801518396 1102 88-89 0.8565004500789009 1102 90-91 0.8385699037552143 1102 92-93 0.8387207814764963 1102 94-95 0.8695460941904152 1102 96-97 0.9368834763797338 1102 98-99 0.9447892401922893 1102 100 0.9927654690353407 1103 1 0.32386965375782495 1103 2 0.2915961790292556 1103 3 0.29021540594221307 1103 4 0.19156723238660334 1103 5 0.28275529273270905 1103 6 0.24030248060817883 1103 7 0.2780613429575922 1103 8 0.2950574612676746 1103 9 0.2997048364418333 1103 10-11 0.3012236994045594 1103 12-13 0.29974987048679935 1103 14-15 0.5151743056803326 1103 16-17 0.5029052143812507 1103 18-19 0.41349999091836054 1103 20-21 0.4407883383151514 1103 22-23 0.4289971395694039 1103 24-25 0.4547484654239824 1103 26-27 0.4792402618062468 1103 28-29 0.4654234003474045 1103 30-31 0.5484343741576581 1103 32-33 0.49914694025756745 1103 34-35 0.5227536715826986 1103 36-37 0.48392505514568285 1103 38-39 0.5461751667861705 1103 40-41 0.5240401032304618 1103 42-43 0.6593222900457718 1103 44-45 0.6979031007275793 1103 46-47 0.7635943671726295 1103 48-49 0.6116182865605637 1103 50-51 0.6368449286915663 1103 52-53 0.6365957823539787 1103 54-55 0.6663447926707633 1103 56-57 0.6659948897710564 1103 58-59 0.651098132748956 1103 60-61 0.6385125927455206 1103 62-63 0.6718345709993514 1103 64-65 0.6307566799769191 1103 66-67 0.6307650252299197 1103 68-69 0.5680090302285166 1103 70-71 0.5190448407808645 1103 72-73 0.5806266159496047 1103 74-75 0.5563570143444636 1103 76-77 0.574738703430512 1103 78-79 0.6107197085459681 1103 80-81 0.55526741876524 1103 82-83 0.516292203545472 1103 84-85 0.5354989527996032 1103 86-87 0.5288946746022205 1103 88-89 0.5714623400094219 1103 90-91 0.6005117441084948 1103 92-93 0.6003952100465924 1103 94-95 0.6131469261236298 1103 96-97 0.6695345124386343 1103 98-99 0.6629927506333075 1103 100 0.6613967318561578 1104 1 0.28728592637637007 1104 2 0.28532697062057366 1104 3 0.268818021848233 1104 4 0.18388684703735692 1104 5 0.1767288728042402 1104 6 0.12673150026279956 1104 7 0.1797125564438531 1104 8 0.1810585362562307 1104 9 0.18353633149547477 1104 10-11 0.309681694743162 1104 12-13 0.28620871281806615 1104 14-15 0.4641784333559187 1104 16-17 0.41252148764819907 1104 18-19 0.43763372151543223 1104 20-21 0.4029328025123675 1104 22-23 0.3761227515072818 1104 24-25 0.3946645932455297 1104 26-27 0.4809130421656036 1104 28-29 0.43059853609882737 1104 30-31 0.4137456326534945 1104 32-33 0.5423453757714825 1104 34-35 0.5219695587343764 1104 36-37 0.4742207162044636 1104 38-39 0.4907340343221449 1104 40-41 0.5030802201778783 1104 42-43 0.47849212640065986 1104 44-45 0.5610623464321307 1104 46-47 0.5209969013801228 1104 48-49 0.5936432157698732 1104 50-51 0.6424235523277346 1104 52-53 0.5200961216712727 1104 54-55 0.6102214254821021 1104 56-57 0.6236809373198042 1104 58-59 0.5783850110652793 1104 60-61 0.5365810513764444 1104 62-63 0.5590393157566709 1104 64-65 0.5200252625278239 1104 66-67 0.4887870123573492 1104 68-69 0.5788729325907767 1104 70-71 0.5483600305471086 1104 72-73 0.5154184155164927 1104 74-75 0.5304089672491372 1104 76-77 0.509396789138119 1104 78-79 0.5444040806566726 1104 80-81 0.560706824673094 1104 82-83 0.6435065966606004 1104 84-85 0.6536345820052993 1104 86-87 0.6129618445738352 1104 88-89 0.6789961037955798 1104 90-91 0.697410072643784 1104 92-93 0.7420496255782965 1104 94-95 0.7463920894856244 1104 96-97 0.8236871590042156 1104 98-99 0.8131644223400372 1104 100 0.8396598872152268 1105 1 0.19370679214658537 1105 2 0.209269417786615 1105 3 0.1834804323050392 1105 4 0.12098639043706783 1105 5 0.19682454243852732 1105 6 0.15904177858647017 1105 7 0.1952332578042828 1105 8 0.14939040461850794 1105 9 0.15961410062443093 1105 10-11 0.17841068272657168 1105 12-13 0.12061210950881218 1105 14-15 0.21739494844904783 1105 16-17 0.2543288434386568 1105 18-19 0.26081395017589415 1105 20-21 0.22878738585004754 1105 22-23 0.1896090408825799 1105 24-25 0.1659562182080876 1105 26-27 0.16826182534874334 1105 28-29 0.21466697496447296 1105 30-31 0.24719925741345605 1105 32-33 0.2514484613187449 1105 34-35 0.29219773118912684 1105 36-37 0.2876463193389256 1105 38-39 0.2960059978256382 1105 40-41 0.3014895105315887 1105 42-43 0.303743531973133 1105 44-45 0.2733261255793096 1105 46-47 0.21768498119988067 1105 48-49 0.20588724047593132 1105 50-51 0.225521744872772 1105 52-53 0.2351817261172613 1105 54-55 0.1800282106113471 1105 56-57 0.21672043265208174 1105 58-59 0.18625806427903058 1105 60-61 0.1821711975610043 1105 62-63 0.2320265659068852 1105 64-65 0.16594524369506303 1105 66-67 0.19506042788188438 1105 68-69 0.2006614902493098 1105 70-71 0.21202715439250852 1105 72-73 0.24442237703414804 1105 74-75 0.2333110362628119 1105 76-77 0.19819244844522288 1105 78-79 0.2454168447016727 1105 80-81 0.25657100472302474 1105 82-83 0.2671494976286368 1105 84-85 0.24446386882515014 1105 86-87 0.2696137476512206 1105 88-89 0.26204943538699865 1105 90-91 0.22598906407242225 1105 92-93 0.23134178119737925 1105 94-95 0.22299143087520434 1105 96-97 0.15378064400175973 1105 98-99 0.13486605638244242 1105 100 0.13127340531138998 1106 1 0.17935762251286747 1106 2 0.1483359881941908 1106 3 0.15049344833590794 1106 4 0.14439208768558842 1106 5 0.21146945690561836 1106 6 0.18098626452430722 1106 7 0.16056591685610044 1106 8 0.12409986664862771 1106 9 0.1615076507805 1106 10-11 0.19767469915193914 1106 12-13 0.20636297932444592 1106 14-15 0.22205133180052172 1106 16-17 0.23097272417729897 1106 18-19 0.19349529877734284 1106 20-21 0.20859816429555877 1106 22-23 0.15965607308316976 1106 24-25 0.1836389083131209 1106 26-27 0.21682705876410324 1106 28-29 0.28456438659566174 1106 30-31 0.2846525836154967 1106 32-33 0.2961165516542579 1106 34-35 0.21733100747043466 1106 36-37 0.2392810583040088 1106 38-39 0.21748790626202918 1106 40-41 0.25289323017450727 1106 42-43 0.26189220753753517 1106 44-45 0.19390549534707446 1106 46-47 0.25954313533607376 1106 48-49 0.34538741541651774 1106 50-51 0.30478786096114874 1106 52-53 0.24744907455932008 1106 54-55 0.2560650531492783 1106 56-57 0.21311272163187311 1106 58-59 0.3055968220655103 1106 60-61 0.26465947553909075 1106 62-63 0.35472812102344875 1106 64-65 0.3869125937160902 1106 66-67 0.34277641935641157 1106 68-69 0.34973707774141616 1106 70-71 0.31777341384694324 1106 72-73 0.28112059837960857 1106 74-75 0.27301933849698656 1106 76-77 0.11663216386851616 1106 78-79 0.1819425628478264 1106 80-81 0.14333478806135957 1106 82-83 0.11469135993385038 1106 84-85 0.15194901078016443 1106 86-87 0.20214759978281194 1106 88-89 0.15632679792455306 1106 90-91 0.11798812621042032 1106 92-93 0.14687376725426304 1106 94-95 0.14764518698496687 1106 96-97 0.13617273598541857 1106 98-99 0.16702791539937678 1106 100 0.2266257689893152 1107 1 0.14532819114736384 1107 2 0.1377564587366109 1107 3 0.14398127668227545 1107 4 0.11491439052801411 1107 5 0.08929007196537242 1107 6 0.1436803894831229 1107 7 0.10398092494308031 1107 8 0.12014108403862878 1107 9 0.11651820878211794 1107 10-11 0.11024031226482123 1107 12-13 0.11195006224593129 1107 14-15 0.20982258258675301 1107 16-17 0.23283628634221287 1107 18-19 0.18761117846104725 1107 20-21 0.1618949190204475 1107 22-23 0.1886812176004966 1107 24-25 0.172278994649119 1107 26-27 0.24311886957369921 1107 28-29 0.23591614776019298 1107 30-31 0.17515777650494613 1107 32-33 0.19434415521252646 1107 34-35 0.1597391210326009 1107 36-37 0.10179725098380032 1107 38-39 0.17336027986552693 1107 40-41 0.18005939889287248 1107 42-43 0.2014059965871695 1107 44-45 0.19970607828354758 1107 46-47 0.22231752169902563 1107 48-49 0.23323719601975057 1107 50-51 0.10289977577829035 1107 52-53 0.09133994151352454 1107 54-55 0.1254790466197946 1107 56-57 0.17753936972843576 1107 58-59 0.199434618213715 1107 60-61 0.23490636242620866 1107 62-63 0.3085717296884951 1107 64-65 0.2967734209093038 1107 66-67 0.1494669444859511 1107 68-69 0.117174628285035 1107 70-71 0.13750232217952885 1107 72-73 0.15305405114565218 1107 74-75 0.17411318677626042 1107 76-77 0.1852938061244842 1107 78-79 0.27742017202205105 1107 80-81 0.25867393578352704 1107 82-83 0.265589585665424 1107 84-85 0.18597446417519947 1107 86-87 0.22553689614067451 1107 88-89 0.22001082913429926 1107 90-91 0.2605389319132385 1107 92-93 0.25191160945332314 1107 94-95 0.25927359025569885 1107 96-97 0.32545463275661746 1107 98-99 0.3929066724754833 1107 100 0.4077212365365632 1108 1 0.08945443214910398 1108 2 0.07976573100148698 1108 3 0.057916958076027925 1108 4 0.07607896691147431 1108 5 0.07154718877117006 1108 6 0.08259214534761838 1108 7 0.11412466766067553 1108 8 0.11178169698442275 1108 9 0.09208124847821608 1108 10-11 0.07211517260225264 1108 12-13 0.08582480586535013 1108 14-15 0.16035492976929788 1108 16-17 0.16601838505837918 1108 18-19 0.18770703021654356 1108 20-21 0.14846918891771566 1108 22-23 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62-63 -0.930290925942522 2316 64-65 -0.869233106342449 2316 66-67 -0.7308730086070767 2316 68-69 -0.667226083481971 2316 70-71 -0.7025585834188348 2316 72-73 -0.7566673775035113 2316 74-75 -0.7630266444239524 2316 76-77 -0.855264125615534 2316 78-79 -0.8303784517862276 2316 80-81 -0.8429548842504282 2316 82-83 -0.8906213025196834 2316 84-85 -0.8452350635989525 2316 86-87 -0.8113489463346184 2316 88-89 -0.7733707715796392 2316 90-91 -0.851318137870404 2316 92-93 -0.790923310119183 2316 94-95 -0.8404921469782032 2316 96-97 -0.8551232562120816 2316 98-99 -0.8937703469185365 2316 100 -0.9216254124991785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 19.0 4 26.0 5 91.0 6 246.0 7 608.0 8 1518.0 9 3237.0 10 5826.0 11 12130.0 12 21356.0 13 31089.0 14 33375.0 15 41262.0 16 54305.0 17 71668.0 18 71416.0 19 45295.0 20 30817.0 21 26487.0 22 28190.0 23 30877.0 24 32752.0 25 32630.0 26 36327.0 27 43539.0 28 53412.0 29 66203.0 30 81967.0 31 101592.0 32 128440.0 33 166891.0 34 236999.0 35 383470.0 36 765138.0 37 1665243.0 38 1542994.0 39 253009.0 40 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.891266792328736 17.72456466093701 13.970768323322883 22.413400223411376 2 13.761228071498877 23.62228483772901 35.76631311244585 26.850173978326257 3 20.390142592765617 29.172653792502036 29.352882262207423 21.08432135252492 4 13.773284195407998 19.464021134793647 32.77573163913163 33.98696303066673 5 12.801567588398546 38.05045781705054 32.22284893848635 16.925125656064562 6 30.566262518541055 32.47074779396494 18.418853987877686 18.54413569961632 7 23.965016192534204 33.98168490543058 21.801804430379644 20.251494471655565 8 29.1387099507905 30.411967509696915 21.526194499570618 18.923128039941968 9 29.301230682833175 14.851235051258099 19.014188283540122 36.83334598236861 10-11 24.620183977087034 27.32832938648679 24.411353804700514 23.640132831725662 12-13 28.167608669959975 25.130195121799296 25.483649203994784 21.21854700424594 14-15 27.37267711265568 22.935962080112436 25.48855032028549 24.202810486946394 16-17 22.884598709219723 26.689061544415505 28.660711783053973 21.765627963310802 18-19 25.237683650482545 25.525005446140092 29.768749269545168 19.468561633832195 20-21 24.472345219597923 25.524720655762106 28.95632694803337 21.046607176606603 22-23 22.53598018996501 27.64812222908955 25.003530422571295 24.81236715837414 24-25 22.951225179526205 28.46103124655506 24.25224768899582 24.335495884922913 26-27 24.317212211125092 25.474326556205355 25.404426922178462 24.804034310491094 28-29 23.745028240511857 24.205149582260734 29.45808477267929 22.59173740454812 30-31 26.252200088421546 24.673603348544006 27.72382708259874 21.350369480435706 32-33 20.65984728307217 26.815987699373945 29.248582391368576 23.2755826261853 34-35 20.04476847355242 27.876513763723764 29.048739606001366 23.029978156722443 36-37 23.675456930743948 27.758529376618984 26.607603024660808 21.958410667976253 38-39 24.810630865460094 25.116213007431504 26.380422351647393 23.69273377546101 40-41 22.602350023014374 27.07950580080177 26.423727357727255 23.894416818456605 42-43 26.08559384143192 25.45798227656086 26.428630791299547 22.027793090707675 44-45 25.6177440421316 24.611351783393633 27.793383956473107 21.97752021800166 46-47 23.10838060310627 25.632840144226734 29.80081987911385 21.457959373553148 48-49 24.06404331909236 26.69273190730175 28.763990758598002 20.479234015007894 50-51 22.42253765852067 27.77191218161656 26.525179307750374 23.28037085211239 52-53 21.483940943726974 28.73154614413722 23.94149616339463 25.843016748741178 54-55 22.784702228231257 27.381506985393194 23.73297746852524 26.100813317850307 56-57 23.826151188634494 24.816410755877584 24.821214975720654 26.536223079767268 58-59 23.40842512176454 24.518681984553197 27.739854853928865 24.333038039753397 60-61 23.969938100621125 25.73600091156283 26.156951117443228 24.137109870372814 62-63 20.10778983138709 26.852011163425722 27.496800888496814 25.543398116690376 64-65 20.013120224217484 26.96397044474429 26.53820244896099 26.48470688207723 66-67 22.912877566865394 26.79559584585575 24.701976056782343 25.589550530496513 68-69 23.79356298099383 25.065763484218895 24.84103333251375 26.299640202273526 70-71 22.23892991092262 26.113475430736816 25.133293151829207 26.514301506511355 72-73 24.20155365456991 25.0240573774086 25.332580282711987 25.441808685309503 74-75 24.52015805391507 24.135473098321118 25.976897429283934 25.367471418479877 76-77 21.870327308467388 25.633207586262742 27.34547190885627 25.1509931964136 78-79 22.206932908361622 25.287688215583533 27.371555488748477 25.13382338730637 80-81 21.220749587537526 25.707218441600805 26.44597089936899 26.626061071492675 82-83 20.984263523507593 26.66382157802757 24.33040084851067 28.02151404995417 84-85 21.659330678919623 25.687857962884596 24.454841567499077 28.197969790696703 86-87 22.232778552291123 23.599397108975257 25.45450000450775 28.71332433422587 88-89 22.093959687538675 23.108344350018317 27.101487641779205 27.696208320663807 90-91 22.74268214392056 23.618029250688682 26.33503690656432 27.304251698826437 92-93 20.42011003478993 24.498952236954434 26.889661634169403 28.191276094086238 94-95 19.74479157574355 24.799842114446864 27.336509894934473 28.11885641487511 96-97 21.490842361750275 24.13555451923526 26.005422377469923 28.368180741544542 98-99 22.259812928161274 23.200753493356693 25.84245517404618 28.696978404435853 100 22.058494482507765 24.140206707409728 26.287864270953214 27.51343453912929 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 336.0 1 235.5 2 192.0 3 250.0 4 216.5 5 282.0 6 519.0 7 973.5 8 1510.0 9 1667.5 10 1370.5 11 1265.5 12 1430.0 13 1587.5 14 1885.5 15 2247.0 16 2659.5 17 3156.0 18 3758.5 19 4471.0 20 5335.0 21 6351.5 22 7632.0 23 9084.0 24 10908.5 25 13460.0 26 16901.0 27 21252.0 28 25810.5 29 32160.0 30 39843.0 31 47594.5 32 56456.5 33 64983.0 34 73965.0 35 82163.5 36 89130.5 37 98054.0 38 103761.5 39 104634.5 40 103366.5 41 101188.0 42 101754.0 43 109040.5 44 121214.0 45 132700.0 46 154203.5 47 196974.5 48 335064.5 49 488661.5 50 648685.5 51 641396.0 52 444096.0 53 318289.0 54 227829.5 55 173064.0 56 142472.0 57 120004.5 58 109829.0 59 100116.5 60 82791.0 61 66706.0 62 54094.0 63 41989.0 64 30980.5 65 24591.0 66 19206.5 67 14309.0 68 10922.5 69 8765.0 70 7273.5 71 6045.5 72 5171.0 73 5193.0 74 4019.5 75 2874.0 76 2144.0 77 1432.5 78 1007.0 79 573.0 80 360.5 81 274.0 82 225.0 83 172.0 84 105.5 85 58.5 86 35.0 87 21.0 88 13.0 89 10.5 90 7.0 91 2.5 92 1.5 93 1.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01135944344300305 2 0.04532303191905257 3 9.998932900767475E-4 4 0.0 5 0.0011474185295962678 6 0.0 7 0.0 8 0.0 9 8.851514371171207E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.934006387187203E-4 22-23 0.0017457153343143216 24-25 0.0035815849816683495 26-27 0.00780244600125462 28-29 0.003196380189589603 30-31 0.005376475395822511 32-33 0.0024341664520720822 34-35 0.006163276673259953 36-37 0.0 38-39 0.0 40-41 0.0018850447271938685 42-43 0.0023276204457524288 44-45 0.0024095789121521623 46-47 0.001926023960393735 48-49 0.001008089136716721 50-51 0.001737519487674348 52-53 0.006581264851898592 54-55 0.003393080508948963 56-57 0.030259065794781576 58-59 0.01756369934946287 60-61 0.0202601328940141 62-63 0.014924636731391454 64-65 0.0023849913722322424 66-67 0.001516231628395068 68-69 1.639169327994668E-5 70-71 0.0 72-73 0.0021555076663129884 74-75 0.0012211811493560278 76-77 7.376261975976006E-4 78-79 0.0027292169311111227 80-81 0.0015736025548748812 82-83 0.001303139615755761 84-85 0.00402416070022691 86-87 7.540178908775474E-4 88-89 9.179348236770142E-4 90-91 6.96646964397734E-4 92-93 5.737092647981339E-4 94-95 4.835549517584271E-4 96-97 9.835015967968007E-5 98-99 0.0 100 0.002360403832312322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6100651.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.49706170972296 #Duplication Level Percentage of deduplicated Percentage of total 1 80.28634007482731 26.090701478594667 2 10.886677697507444 7.075700738995281 3 3.3398635314946037 3.2560725385510025 4 1.4833509292439102 1.9281818673925704 5 0.8062741221227253 1.3100769950787456 6 0.5382390296141428 1.0494712175971321 7 0.39257789346300637 0.8930339620818255 8 0.27685029176976134 0.7197456812797395 9 0.21033770781908476 0.6151821723790628 >10 1.433598981472508 9.100957479741364 >50 0.1685506232992849 3.821863260793432 >100 0.14878215710899215 9.759158811828353 >500 0.016412630264196072 3.7036972357927946 >1k 0.008689039451207138 5.458211322074832 >5k 0.0012195143089413524 2.690011694088854 >10k+ 0.0022357762330591463 22.527933543730256 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 147841 2.423364326200597 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 122881 2.0142276619331283 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 88639 1.4529433006411938 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 69735 1.1430747308770817 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 61649 1.0105314990154328 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 61059 1.0008603999802645 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 57223 0.9379818645583891 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 56800 0.9310481783009715 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 54924 0.9002973617077916 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 47092 0.7719176199392491 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 43468 0.7125141234927224 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 41684 0.6832713426812974 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 26956 0.4418544840542427 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 26095 0.42774123614020865 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 23679 0.3881389051758575 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 22600 0.370452268126795 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 21944 0.3596993173351499 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 21936 0.35956818378891037 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 21327 0.3495856425814229 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18785 0.30791795826379836 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 17765 0.2911984311182528 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 17534 0.2874119499705851 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 17495 0.2867726739326672 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 16931 0.27752775892277726 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 16877 0.2766426074856601 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 16703 0.2737904528549494 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 16297 0.2671354253832911 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 16007 0.2623818343321065 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 14955 0.2451377730016026 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14847 0.2433674701273684 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 13448 0.22043549122872297 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 13049 0.21389520561002426 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 12852 0.21066604203387476 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 12460 0.20424049826813562 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 12088 0.19814278836799548 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11990 0.1965364024265607 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 11948 0.19584795130880295 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 11335 0.18579984332819563 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 11164 0.18299686377732477 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10933 0.17921038262965705 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 10413 0.1706867021240848 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 10343 0.16953928359448853 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 10343 0.16953928359448853 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 10180 0.16686743758985723 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 9164 0.1502134772174314 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 9146 0.14991842673839234 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 8753 0.1434764912793733 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA 8621 0.14131278776642034 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 8556 0.14024732770322382 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8445 0.13842784974914973 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 8400 0.13769022355155214 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 7348 0.12044616222104822 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT 7098 0.11634823890106154 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 6673 0.1093817692570842 No Hit GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA 6558 0.10749672452989034 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT 6524 0.10693940695837213 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG 6513 0.10675909833229275 No Hit ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT 6355 0.10416921079406116 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.008245021719813181 0.0 0.0 3.278338655989336E-5 0.0 2 0.011965936094361078 0.0 0.0 3.278338655989336E-5 0.0 3 0.012605212132278998 0.0 0.0 3.278338655989336E-5 0.0 4 0.015195099670510573 1.639169327994668E-5 0.0 4.9175079839840044E-5 0.0 5 0.05771515203869226 1.639169327994668E-5 0.0 4.5896741183850705E-4 0.0 6 0.12501944464615333 1.639169327994668E-5 0.0 8.359763572772808E-4 0.0 7 0.14314865741377436 1.639169327994668E-5 0.0 9.998932900767475E-4 0.0 8 0.16899835771625027 3.278338655989336E-5 0.0 0.0011965936094361076 0.0 9 0.18753736281586997 8.19584663997334E-5 0.0 0.0014260773153553612 3.278338655989336E-5 10-11 0.3661576444874489 8.19584663997334E-5 0.0 0.003122617569829843 3.278338655989336E-5 12-13 0.6154097325023182 8.19584663997334E-5 0.0 0.005925597120700725 3.278338655989336E-5 14-15 0.8851514371171207 8.19584663997334E-5 0.0 0.009482594562449155 4.9175079839840044E-5 16-17 0.9939840846493269 8.19584663997334E-5 0.0 0.011326660056443158 4.9175079839840044E-5 18-19 1.0422740130520496 9.835015967968007E-5 0.0 0.01239212011963969 6.556677311978673E-5 20-21 1.2046501266832015 1.3113354623957345E-4 0.0 0.0160474677210678 6.556677311978673E-5 22-23 1.25121892729153 1.3113354623957345E-4 0.0 0.02180914790896906 8.19584663997334E-5 24-25 1.4623685242771631 1.3113354623957345E-4 0.0 0.040479286554828325 8.19584663997334E-5 26-27 1.929343278282924 1.3113354623957345E-4 0.0 0.09249012933209916 8.19584663997334E-5 28-29 2.0417165315635986 1.393293928795468E-4 0.0 0.17290777656351758 8.19584663997334E-5 30-31 2.0809746369690707 1.4752523951952012E-4 0.0 0.34927420040910384 8.19584663997334E-5 32-33 2.1159872938150373 1.4752523951952012E-4 0.0 0.6076892449674633 8.19584663997334E-5 34-35 2.1430909586534286 1.639169327994668E-4 0.0 0.928146848590421 8.19584663997334E-5 36-37 2.189831872041197 1.803086260794135E-4 0.0 1.3783856837573563 8.19584663997334E-5 38-39 2.2365481978890447 1.803086260794135E-4 0.0 2.0461422887491842 8.19584663997334E-5 40-41 2.2803631940263425 2.1309201263930685E-4 0.0 3.094202569529055 8.19584663997334E-5 42-43 2.373631928789239 2.1309201263930685E-4 0.0 3.7931033917527817 8.19584663997334E-5 44-45 2.475448931597628 2.212878592792802E-4 0.0 4.449377615602007 8.19584663997334E-5 46-47 2.5112074104878315 2.2948370591925352E-4 0.0 5.195109505526542 8.19584663997334E-5 48-49 2.5325084159051223 2.3767955255922686E-4 0.0 5.835369045041259 9.835015967968009E-5 50-51 2.5765283082084194 3.114421723189869E-4 0.0 6.564225686734088 9.835015967968009E-5 52-53 2.5910267609145317 3.114421723189869E-4 0.0 7.518402544253064 9.835015967968009E-5 54-55 2.6396609148761336 3.114421723189869E-4 0.0 8.2638885587784 1.0654600631965342E-4 56-57 2.7340442847820667 3.114421723189869E-4 0.0 8.990237271399398 1.229376995996001E-4 58-59 2.7643853090432478 3.114421723189869E-4 0.0 9.734920092954013 1.3113354623957345E-4 60-61 2.785940385706378 3.114421723189869E-4 0.0 10.34128980661244 1.4752523951952012E-4 62-63 2.804692482818637 3.278338655989336E-4 0.0 10.95981396083795 1.4752523951952012E-4 64-65 2.8196499029365882 3.442255588788803E-4 0.0 11.640380674128057 1.5572108615949346E-4 66-67 2.8402542613894814 3.9340063871872035E-4 0.0 12.29390928935289 1.803086260794135E-4 68-69 2.863235415367966 3.9340063871872035E-4 0.0 13.103732700002016 1.803086260794135E-4 70-71 2.8927978341983502 3.9340063871872035E-4 0.0 14.128057808912525 1.9670031935936018E-4 72-73 2.9658801986869925 3.9340063871872035E-4 0.0 14.885944139404138 1.9670031935936018E-4 74-75 3.0393149845811536 4.015964853586937E-4 0.0 15.590762362901927 1.9670031935936018E-4 76-77 3.062337117792839 4.1798817863864034E-4 0.0 16.329880204588 2.048961659993335E-4 78-79 3.0769093331187114 4.261840252786137E-4 0.0 17.00361158178037 2.1309201263930685E-4 80-81 3.108668238848608 4.425757185585604E-4 0.0 17.77294751002803 2.3767955255922686E-4 82-83 3.121248863440967 4.753591051184537E-4 0.0 18.749441657947653 2.622670924791469E-4 84-85 3.165285147437544 4.917507983984005E-4 0.0 19.587557131197965 2.622670924791469E-4 86-87 3.2415720879624157 4.917507983984005E-4 0.0 20.36662972525391 2.622670924791469E-4 88 3.2595373837972375 4.917507983984005E-4 0.0 20.98261316702103 2.786587857590936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 3265 0.0 37.44702 1 TCCGATC 3280 0.0 31.377338 3 TATCACG 365 0.0 30.916624 2 AGTACTC 3590 0.0 29.453297 5 ATCTACA 4200 0.0 29.107023 2 CGATCTC 3210 0.0 28.98718 5 CCGATCT 3640 0.0 28.919611 4 TCTACAC 5110 0.0 26.94589 3 TCTCGCG 1405 0.0 24.751076 8 TACACTC 5465 0.0 24.335602 5 AAGTACT 4280 0.0 23.826584 4 CTACACT 6175 0.0 23.287899 4 CTCGCGC 1560 0.0 22.593077 9 AAAGTAC 4515 0.0 22.482357 3 AAAAAGT 4515 0.0 21.663704 1 GATGTAA 2375 0.0 21.383917 1 TTCCGAT 4960 0.0 21.04481 2 GTACTAG 1000 0.0 20.690918 1 AAAAGTA 4910 0.0 20.588783 2 ATCACGC 555 0.0 20.321888 3 >>END_MODULE