FastQCFastQC Report
Fri 27 May 2016
ERR765296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14203804
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA329120.23171257502567622No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT321780.22654494528367186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254140.1789238995412778No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA165590.11658144536491773No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA158340.11147717893037667No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA142080.10002954138201288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG52650.025.524491
GTGATCG48200.023.1009378
GTATCAA811650.022.9716571
GTATAAG65450.022.183921
TATTCGC10500.021.924779
GTACTAG63400.021.4189431
TGATCGC53350.020.870959
CTATTGA79450.020.6967649
CATGGGG414550.020.4700094
GTACATA32850.020.3115181
GTACAAA53050.020.0176221
ATGGGAG133450.019.8929625
AGGGCTA87000.019.658565
GTACATG1276300.019.6450211
GGGCTAT91250.019.5649626
TACATGG1264200.019.3200572
CAACGCA910800.019.2215945
TTTAGGG91250.019.1556992
ATCAACG920800.018.9827633
ATTTAGG91100.018.9809911
Produced by FastQC (version 0.11.5)