##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765296_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14203804
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.866649244103904	33.0	33.0	33.0	27.0	33.0
2	31.770319415840994	33.0	33.0	33.0	27.0	33.0
3	31.792988695141105	33.0	33.0	33.0	27.0	33.0
4	35.53977075436975	37.0	37.0	37.0	33.0	37.0
5	35.62515816185579	37.0	37.0	37.0	33.0	37.0
6	35.596535195782764	37.0	37.0	37.0	33.0	37.0
7	35.52506511635897	37.0	37.0	37.0	33.0	37.0
8	35.50581590678103	37.0	37.0	37.0	33.0	37.0
9	35.45586963886576	37.0	37.0	37.0	33.0	37.0
10-11	35.44896852983891	37.0	37.0	37.0	33.0	37.0
12-13	35.40291991497489	37.0	37.0	37.0	33.0	37.0
14-15	36.966757320785334	40.0	37.0	40.0	33.0	40.0
16-17	37.02594104368097	40.0	37.0	40.0	33.0	40.0
18-19	37.01871283918027	40.0	37.0	40.0	33.0	40.0
20-21	37.12156951053394	40.0	37.0	40.0	33.0	40.0
22-23	37.116076298997086	40.0	37.0	40.0	33.0	40.0
24-25	37.102121868198125	40.0	37.0	40.0	33.0	40.0
26-27	37.06260136369102	40.0	37.0	40.0	33.0	40.0
28-29	37.00681982798411	40.0	37.0	40.0	33.0	40.0
30-31	36.910185926249056	37.0	37.0	40.0	33.0	40.0
32-33	36.781384163003096	37.0	37.0	40.0	33.0	40.0
34-35	36.65247176038194	37.0	37.0	40.0	33.0	40.0
36-37	36.5429575063131	37.0	37.0	40.0	33.0	40.0
38-39	36.41583525793513	37.0	37.0	40.0	33.0	40.0
40-41	36.291157601160926	37.0	37.0	40.0	33.0	40.0
42-43	36.12352676789964	37.0	37.0	40.0	30.0	40.0
44-45	35.94751659485023	37.0	37.0	40.0	27.0	40.0
46-47	35.72944941369228	37.0	37.0	40.0	27.0	40.0
48-49	35.454339309385006	37.0	35.0	40.0	27.0	40.0
50-51	35.32869275723602	37.0	33.0	40.0	27.0	40.0
52-53	35.161721465601744	37.0	33.0	40.0	27.0	40.0
54-55	34.99020132212469	37.0	33.0	40.0	27.0	40.0
56-57	34.817403774369176	37.0	33.0	40.0	27.0	40.0
58-59	34.64230152711204	37.0	33.0	40.0	27.0	40.0
60-61	34.45301843083726	37.0	33.0	37.0	27.0	40.0
62-63	34.23183363414476	37.0	33.0	37.0	27.0	40.0
64-65	34.03866745837946	37.0	33.0	37.0	27.0	40.0
66-67	33.849075747595506	37.0	33.0	37.0	27.0	40.0
68-69	33.659313378303445	37.0	33.0	37.0	27.0	40.0
70-71	33.47734399179262	37.0	33.0	37.0	27.0	40.0
72-73	33.26727135913731	37.0	33.0	37.0	27.0	37.0
74-75	33.051905461382034	37.0	33.0	37.0	24.5	37.0
76-77	32.86333013325163	37.0	33.0	37.0	22.0	37.0
78-79	32.70283534608053	37.0	33.0	37.0	22.0	37.0
80-81	32.53419119272556	37.0	33.0	37.0	22.0	37.0
82-83	32.39414064711116	35.0	33.0	37.0	22.0	37.0
84-85	32.24250295906646	33.0	33.0	37.0	22.0	37.0
86-87	32.11220110471814	33.0	33.0	37.0	22.0	37.0
88-89	31.980777086194657	33.0	33.0	37.0	22.0	37.0
90-91	31.84000141793001	33.0	33.0	37.0	22.0	37.0
92-93	31.714334659926312	33.0	33.0	37.0	22.0	37.0
94-95	31.588985704111376	33.0	33.0	37.0	22.0	37.0
96-97	31.492341136219565	33.0	33.0	37.0	15.0	37.0
98-99	31.364598561061527	33.0	33.0	37.0	15.0	37.0
100	31.28687941624652	33.0	33.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	10.0
7	213.0
8	1077.0
9	2289.0
10	3356.0
11	5587.0
12	10831.0
13	21669.0
14	36045.0
15	43385.0
16	47684.0
17	56541.0
18	67958.0
19	79384.0
20	91585.0
21	107704.0
22	124686.0
23	121346.0
24	114312.0
25	120014.0
26	135376.0
27	160055.0
28	199111.0
29	253243.0
30	328075.0
31	426264.0
32	563615.0
33	741589.0
34	972120.0
35	1349358.0
36	2282055.0
37	3894880.0
38	1839724.0
39	2658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.830413825485245	20.016207021747096	13.022929245956623	24.130449906811034
2	15.986468150918363	21.563575195750005	37.734293970324586	24.715662683007043
3	17.794592974825623	27.803976274411134	29.375360357921448	25.0260703928418
4	12.537468558234801	17.78829087049281	37.572450008244594	32.10179056302779
5	13.507358471508207	38.400481055787004	33.09651981185461	14.995640660850176
6	29.792645688436703	38.54688504572437	17.878837246698136	13.781632019140789
7	26.015671576431217	32.97378645889509	22.837466639218622	18.173075325455066
8	26.716343030360036	32.48655782634004	20.35182265257955	20.445276490720374
9	27.25606464296466	14.630749621721053	20.464820550889044	37.648365184425245
10-11	25.380169284228366	26.049859600991397	26.53201916894939	22.03795194583085
12-13	25.94942875866212	24.383721431244755	26.805836661784404	22.86101314830872
14-15	24.225760225922578	24.664850345724286	24.806101238794902	26.303288189558234
16-17	23.21909329847305	26.846403498569533	25.648170468808658	24.28633273414876
18-19	23.434143890001753	26.176704000937228	27.962240765914377	22.42691134314664
20-21	25.47000286861541	25.08645888560223	26.459888156737005	22.98365008904536
22-23	24.65795726756714	24.970466002441857	26.35040547068416	24.021171259306843
24-25	23.631583321982834	25.89132439828314	26.762651796688324	23.714440483045703
26-27	23.97417329183544	25.68086192385868	26.556150591444894	23.78881419286098
28-29	23.27989492827972	25.622886266806173	27.266645897570708	23.8305729073434
30-31	23.998059132040073	25.495689945040663	26.902607961220387	23.603642961698874
32-33	23.561778363113593	25.729426329437004	26.186625267551968	24.52217003989744
34-35	24.0962092545936	25.53951847350882	27.087627219979442	23.276645051918138
36-37	23.967073169958876	25.504812646631393	26.5255526866811	24.00256149672863
38-39	24.153114390218594	24.99202948795459	26.681387218020358	24.173468903806455
40-41	23.76346030296719	25.37941676025929	27.0941626551981	23.762960281575428
42-43	24.324720368736646	25.09887424318703	26.653297652427906	23.923107735648415
44-45	23.74266180986509	25.721506838415113	26.316121168420835	24.219710183298957
46-47	23.425629754611712	25.430749552509724	26.68256397467627	24.461056718202297
48-49	24.17657577703963	25.700373907347977	26.11204377100157	24.01100654461082
50-51	23.44793257291058	26.038522603597723	25.616592816279937	24.89695200721176
52-53	23.287494354239627	25.824650737514915	25.572823504138032	25.315031404107422
54-55	23.620207840341774	25.800427567938428	25.759565129287097	24.8197994624327
56-57	23.869566792503207	25.15461058896424	25.436632921274317	25.53918969725824
58-59	23.37354096754493	25.234298274774638	26.146603068461534	25.245557689218895
60-61	23.523817004673546	25.43137647091588	25.862963309696667	25.181843214713908
62-63	23.209248956421796	25.32801278070596	25.489806704638525	25.972931558233718
64-65	23.01643840448798	25.28875897004545	25.811368161671016	25.883434463795556
66-67	23.780393195688543	25.20809253663041	25.61648444893832	25.39502981874273
68-69	23.712165153786806	24.96367243612735	25.543022974341383	25.781139435744464
70-71	23.100402899819734	25.194384271318267	25.74906330122123	25.956149527640765
72-73	23.813659461067118	25.260605896740017	25.612573833173926	25.313160809018942
74-75	24.03168277794597	25.251723191089393	25.626579738511474	25.090014292453162
76-77	23.506041595546325	25.51643092411739	25.605301059200062	25.372226421136222
78-79	23.537636017761216	25.7713342430726	25.411972798225552	25.27905694094063
80-81	23.246124860249676	25.461726549570983	26.024050978298906	25.26809761188043
82-83	23.661398475946825	25.433984267345366	25.541286768980587	25.36333048772722
84-85	23.71303229603804	25.673581303690764	24.97105103726596	25.64233536300524
86-87	23.34374428286936	25.330708670564466	25.466840844693255	25.858706201872916
88-89	23.542303445834527	24.89995523683292	25.447652792150073	26.110088525182483
90-91	23.764857011271616	24.991657977316386	25.337596263280748	25.90588874813125
92-93	23.66770271542308	25.163350698127235	25.219051196935883	25.949895389513806
94-95	22.970705280153542	25.345824772399283	25.657772800070973	26.025697147376203
96-97	23.52043060656769	25.20527201851475	25.115206773047266	26.15909060187029
98-99	23.22239794135792	25.439898335185863	25.189626175873443	26.148077547582776
100	23.377671043409418	25.439538254167903	24.773152504473376	26.409638197949302
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1783.0
1	1459.0
2	1486.0
3	1783.5
4	1411.0
5	1204.0
6	1541.5
7	2899.0
8	4597.0
9	4344.0
10	2708.0
11	1877.0
12	1906.0
13	2177.0
14	2655.5
15	3242.0
16	3918.5
17	4761.5
18	5926.0
19	7275.5
20	9018.5
21	11277.0
22	14358.5
23	18323.5
24	23052.0
25	29726.5
26	39262.5
27	50029.5
28	62144.0
29	81981.5
30	105315.0
31	126595.5
32	152807.5
33	179334.0
34	205587.0
35	225518.0
36	243374.5
37	276774.5
38	292890.0
39	291489.0
40	291177.5
41	291658.5
42	302498.5
43	336248.0
44	388155.0
45	440992.5
46	505110.5
47	570808.0
48	633641.0
49	673371.0
50	705581.5
51	693342.0
52	634963.0
53	611649.5
54	581997.5
55	538548.5
56	517474.5
57	480995.0
58	438453.5
59	403255.5
60	347028.0
61	267059.5
62	201735.0
63	156436.5
64	113946.5
65	96493.5
66	88281.5
67	68914.5
68	50956.5
69	40192.5
70	33911.0
71	29928.0
72	27446.5
73	30292.5
74	23644.5
75	16198.0
76	13487.0
77	12255.5
78	10281.0
79	6903.5
80	5407.5
81	4785.5
82	4902.0
83	5213.0
84	4144.5
85	2670.5
86	1883.5
87	931.0
88	432.0
89	328.0
90	268.5
91	196.5
92	124.5
93	91.0
94	67.0
95	39.0
96	34.0
97	23.5
98	10.0
99	3.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01796701784958452
2	0.006103998618961512
3	0.006413774788781934
4	0.003956686532706309
5	0.04715638148766345
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.942605797714471E-4
18-19	0.0016333652590531382
20-21	6.160321558928861E-4
22-23	0.0047944902647206345
24-25	0.007381825319470755
26-27	0.005861105940352317
28-29	0.022655902601866375
30-31	0.020705720805496895
32-33	0.013306294567286341
34-35	0.013566788164635333
36-37	0.013735756984537382
38-39	0.021279510756414266
40-41	0.031058581208245343
42-43	0.018005739870812075
44-45	0.01699544713514774
46-47	0.01870977662040394
48-49	0.013306294567286341
50-51	0.010145169561618846
52-53	0.028087546124967652
54-55	0.02620072763606144
56-57	0.013478783570936348
58-59	0.016580065452888537
60-61	0.01753403524858552
62-63	0.021286551123910185
64-65	0.011296269647201552
66-67	0.015837306682069112
68-69	0.0076740005705513824
70-71	0.008913105249833072
72-73	0.010673197123812747
74-75	0.0035483452179430244
76-77	0.007445188626934025
78-79	0.005868146307848236
80-81	0.0024535680723276666
82-83	2.428926786091951E-4
84-85	0.002527491931034813
86-87	0.0023127607224092926
88-89	0.0011405395343388291
90-91	8.061220782826911E-4
92-93	0.0011334991668429106
94-95	0.0033547351118052597
96-97	0.0014925579091347643
98-99	0.0018762579376623333
100	0.002245877231198065
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4203804E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.83322204460945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.86138375669432	20.001338505329645
2	14.155211882163218	7.8797031080948585
3	5.481943172826574	4.5774042469563865
4	2.6040044431066276	2.8991133548054533
5	1.4516106243203553	2.02015004145113
6	0.9193149823142562	1.5352498819013343
7	0.5960919494575164	1.1613811711778619
8	0.40155732293608687	0.8941307306335239
9	0.31825415788768313	0.7972234778797271
>10	1.776433798239321	9.22935515149071
>50	0.181274184628995	3.5321200176192615
>100	0.17754517668058348	10.745627317620032
>500	0.03521067614162585	6.822127043148008
>1k	0.036850863746352026	20.259817168117667
>5k	0.002701376452110084	5.167508825955098
>10k+	6.116324042513397E-4	2.477749957819473
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	32912	0.23171257502567622	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	32178	0.22654494528367186	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25414	0.1789238995412778	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	16559	0.11658144536491773	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	15834	0.11147717893037667	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA	14208	0.10002954138201288	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.4080734991837397E-5	0.0	0.0	0.0	0.0
3	1.4080734991837397E-5	0.0	0.0	0.0	0.0
4	1.4080734991837397E-5	7.040367495918699E-6	0.0	0.0	0.0
5	2.11211024877561E-5	7.040367495918699E-6	0.0	0.0	0.0
6	2.11211024877561E-5	7.040367495918699E-6	0.0	0.0	0.0
7	2.8161469983674794E-5	1.4080734991837397E-5	0.0	0.0	0.0
8	2.8161469983674794E-5	1.4080734991837397E-5	0.0	0.0	0.0
9	2.8161469983674794E-5	5.632293996734959E-5	0.0	0.0	0.0
10-11	2.8161469983674794E-5	5.632293996734959E-5	0.0	3.5201837479593493E-6	0.0
12-13	3.52018374795935E-5	5.632293996734959E-5	0.0	2.8161469983674794E-5	0.0
14-15	5.280275621939024E-5	8.44844099510244E-5	0.0	2.8161469983674794E-5	0.0
16-17	7.0403674959187E-5	8.44844099510244E-5	0.0	4.22422049755122E-5	0.0
18-19	7.0403674959187E-5	9.504496119490245E-5	0.0	4.928257247143089E-5	0.0
20-21	1.0560551243878049E-4	1.0560551243878049E-4	0.0	1.40807349918374E-4	3.5201837479593493E-6
22-23	1.0560551243878049E-4	1.3024679867449594E-4	0.0	7.638798733071788E-4	1.0560551243878047E-5
24-25	1.4432753366633332E-4	1.3376698242245527E-4	0.0	0.0030414387582368783	1.4080734991837397E-5
26-27	2.1825139237347966E-4	1.3376698242245527E-4	0.0	0.005463325176832911	2.11211024877561E-5
28-29	2.2881194361735772E-4	1.3376698242245527E-4	0.0	0.020934532749114252	2.11211024877561E-5
30-31	2.3233212736531708E-4	1.4784771741429268E-4	0.0	0.08225613363856613	2.11211024877561E-5
32-33	2.3233212736531708E-4	1.4784771741429268E-4	0.0	0.2012665057895758	2.11211024877561E-5
34-35	2.3233212736531708E-4	1.7248900365000812E-4	0.0	0.35127561602511553	2.11211024877561E-5
36-37	2.3233212736531708E-4	1.936101061377642E-4	0.0	0.5921371486117382	2.11211024877561E-5
38-39	2.3233212736531708E-4	2.0417065738164228E-4	0.0	0.9897700644137304	2.11211024877561E-5
40-41	2.3937249486123577E-4	2.3937249486123577E-4	0.0	1.485376734288927	2.11211024877561E-5
42-43	2.5345322985307315E-4	2.569734136010325E-4	0.0	1.905204408621803	2.11211024877561E-5
44-45	2.675339648449106E-4	2.851348835847073E-4	0.0	2.3750890958506607	2.11211024877561E-5
46-47	2.81614699836748E-4	3.1329635356838205E-4	0.0	2.8900356552371465	2.11211024877561E-5
48-49	2.81614699836748E-4	3.2385690481226013E-4	0.0	3.4430741229603	2.8161469983674794E-5
50-51	2.81614699836748E-4	4.0130094726736584E-4	0.0	4.032145191527565	2.8161469983674794E-5
52-53	2.81614699836748E-4	4.2242204975512194E-4	0.0	4.597592306962276	2.8161469983674794E-5
54-55	2.81614699836748E-4	4.294624172510406E-4	0.0	5.137690579227931	2.8161469983674794E-5
56-57	2.81614699836748E-4	4.4354315224287805E-4	0.0	5.744221759185075	2.8161469983674794E-5
58-59	2.81614699836748E-4	4.4354315224287805E-4	0.0	6.415408154040988	2.8161469983674794E-5
60-61	2.81614699836748E-4	4.470633359908374E-4	0.0	7.113076891232799	2.8161469983674794E-5
62-63	2.81614699836748E-4	4.822651734704309E-4	0.0	7.810185919208685	2.8161469983674794E-5
64-65	2.81614699836748E-4	5.315477459418617E-4	0.0	8.493365580093895	3.52018374795935E-5
66-67	2.81614699836748E-4	5.421082971857398E-4	0.0	9.206445681734273	5.632293996734959E-5
68-69	2.81614699836748E-4	5.667495834214553E-4	0.0	10.003144228123677	5.632293996734959E-5
70-71	2.81614699836748E-4	5.913908696571707E-4	0.0	10.89149427857495	5.632293996734959E-5
72-73	2.8865506733266664E-4	6.054716046490082E-4	0.0	11.73491622385102	5.632293996734959E-5
74-75	2.8865506733266664E-4	6.195523396408455E-4	0.0	12.542097877441847	5.632293996734959E-5
76-77	2.8865506733266664E-4	6.301128908847236E-4	0.0	13.388061395383941	5.984312371530894E-5
78-79	2.92175251080626E-4	6.512339933724796E-4	0.0	14.255916936054595	6.336330746326829E-5
80-81	2.9569543482858536E-4	6.723550958602357E-4	0.0	15.20284636425566	7.0403674959187E-5
82-83	2.9569543482858536E-4	6.899560146000325E-4	0.0	16.189715797261073	7.392385870714634E-5
84-85	2.9569543482858536E-4	6.899560146000325E-4	0.0	17.11667874324371	7.744404245510569E-5
86-87	2.9569543482858536E-4	7.075569333398293E-4	0.0	18.046690168352082	7.744404245510569E-5
88	2.9569543482858536E-4	7.25157852079626E-4	0.0	18.753497302553598	7.744404245510569E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAG	5265	0.0	25.52449	1
GTGATCG	4820	0.0	23.100937	8
GTATCAA	81165	0.0	22.971657	1
GTATAAG	6545	0.0	22.18392	1
TATTCGC	1050	0.0	21.92477	9
GTACTAG	6340	0.0	21.418943	1
TGATCGC	5335	0.0	20.87095	9
CTATTGA	7945	0.0	20.696764	9
CATGGGG	41455	0.0	20.470009	4
GTACATA	3285	0.0	20.311518	1
GTACAAA	5305	0.0	20.017622	1
ATGGGAG	13345	0.0	19.892962	5
AGGGCTA	8700	0.0	19.65856	5
GTACATG	127630	0.0	19.645021	1
GGGCTAT	9125	0.0	19.564962	6
TACATGG	126420	0.0	19.320057	2
CAACGCA	91080	0.0	19.221594	5
TTTAGGG	9125	0.0	19.155699	2
ATCAACG	92080	0.0	18.982763	3
ATTTAGG	9110	0.0	18.980991	1
>>END_MODULE