##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765295_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7751566
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.295043220944	33.0	31.0	34.0	31.0	34.0
2	32.32743693338868	34.0	31.0	34.0	31.0	34.0
3	32.343638691846266	34.0	31.0	34.0	31.0	34.0
4	35.81190020184309	37.0	35.0	37.0	35.0	37.0
5	35.84431765142682	37.0	35.0	37.0	35.0	37.0
6	35.94197004837474	37.0	37.0	37.0	35.0	37.0
7	35.90026454009422	37.0	37.0	37.0	35.0	37.0
8	35.93168206269546	37.0	37.0	37.0	35.0	37.0
9	37.63049066472504	39.0	38.0	39.0	35.0	39.0
10-11	37.572085111576165	39.0	38.0	39.0	35.0	39.0
12-13	37.54970950386026	39.0	38.0	39.0	35.0	39.0
14-15	38.93790235418237	41.0	39.0	41.0	36.0	41.0
16-17	38.904191682042054	41.0	39.0	41.0	36.0	41.0
18-19	38.90298592568263	41.0	39.0	41.0	35.5	41.0
20-21	38.88309955691534	41.0	39.0	41.0	35.0	41.0
22-23	38.775493235818416	40.5	39.0	41.0	35.0	41.0
24-25	38.69124084088299	41.0	38.5	41.0	35.0	41.0
26-27	38.56475497983246	40.0	38.0	41.0	34.5	41.0
28-29	38.424013612217195	40.0	38.0	41.0	34.0	41.0
30-31	38.1956702684335	40.0	38.0	41.0	34.0	41.0
32-33	37.94113279819845	40.0	38.0	41.0	33.5	41.0
34-35	37.771394515637226	40.0	37.0	41.0	33.0	41.0
36-37	37.594811809639495	40.0	37.0	41.0	33.0	41.0
38-39	37.390810192417895	40.0	37.0	41.0	33.0	41.0
40-41	37.12789015793712	40.0	36.5	41.0	32.0	41.0
42-43	36.84140720726624	40.0	36.0	41.0	31.0	41.0
44-45	36.473198060882154	40.0	35.5	41.0	30.5	41.0
46-47	36.00627847327882	39.0	35.0	41.0	29.5	41.0
48-49	35.56296076173511	39.0	35.0	41.0	27.5	41.0
50-51	34.269394210150566	38.0	34.0	40.0	25.0	40.5
52-53	34.18211900666265	38.0	34.0	39.5	24.5	40.0
54-55	34.9831066393552	39.0	35.0	40.5	26.0	41.0
56-57	34.997352599461834	39.0	35.0	41.0	26.0	41.0
58-59	34.77577363077344	38.0	35.0	41.0	26.0	41.0
60-61	34.44478051273769	37.5	35.0	40.0	26.0	41.0
62-63	34.02272527899524	37.0	34.5	40.0	24.0	41.0
64-65	33.69526118980345	36.0	34.0	39.5	23.0	41.0
66-67	33.39739937710651	36.0	34.0	39.0	23.0	41.0
68-69	33.145343405448656	35.5	34.0	39.0	22.0	41.0
70-71	32.78904430407998	35.0	34.0	38.5	21.5	40.5
72-73	32.41810422564937	35.0	34.0	37.0	20.0	39.5
74-75	32.03530622070431	35.0	34.0	37.0	18.5	39.0
76-77	31.684917537952977	35.0	33.0	36.0	16.0	39.0
78-79	31.390637969153588	35.0	33.0	36.0	12.0	37.0
80-81	31.094255792958478	35.0	33.0	35.5	7.5	37.0
82-83	30.842942703448568	35.0	33.0	35.0	3.5	36.5
84-85	30.605872542399823	35.0	33.0	35.0	2.0	36.0
86-87	30.35733644014642	35.0	33.0	35.0	2.0	36.0
88-89	30.151753336035583	35.0	32.0	35.0	2.0	36.0
90-91	29.923502296181184	35.0	32.0	35.0	2.0	35.0
92-93	29.664543267773247	35.0	32.0	35.0	2.0	35.0
94-95	29.377836749374254	35.0	31.0	35.0	2.0	35.0
96-97	29.109918563552192	35.0	31.0	35.0	2.0	35.0
98-99	28.812973404341783	35.0	31.0	35.0	2.0	35.0
100	28.294943112140178	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3861081492720331
1101	2	0.4871556705495266
1101	3	0.46649154506584267
1101	4	0.3389016699246028
1101	5	0.4015350961271693
1101	6	0.38011856546921763
1101	7	0.4698961072363801
1101	8	0.42009713126993375
1101	9	0.5212705056896567
1101	10-11	0.5077884788415901
1101	12-13	0.4668320115988678
1101	14-15	0.7341582697042028
1101	16-17	0.7012511872007465
1101	18-19	0.6866616700814276
1101	20-21	0.7130889708683554
1101	22-23	0.7002787858495623
1101	24-25	0.7215768706707095
1101	26-27	0.7420957464485625
1101	28-29	0.8286633656279534
1101	30-31	0.8595132972181503
1101	32-33	0.8280781873640919
1101	34-35	0.9082930299627137
1101	36-37	1.0008986855377273
1101	38-39	1.0034851530594437
1101	40-41	1.0238226255437795
1101	42-43	1.0446710125310759
1101	44-45	1.0776037405235925
1101	46-47	1.1981560650953895
1101	48-49	1.3117583109699709
1101	50-51	1.2571437323065098
1101	52-53	1.3170964030567873
1101	54-55	1.4089083899033668
1101	56-57	1.4490419395745917
1101	58-59	1.448127685463227
1101	60-61	1.4073114110465283
1101	62-63	1.393833307088407
1101	64-65	1.3696206822929398
1101	66-67	1.3915002552085909
1101	68-69	1.3578620051774664
1101	70-71	1.3694278155006927
1101	72-73	1.3433168937855626
1101	74-75	1.2574625520497236
1101	76-77	1.2345451945089394
1101	78-79	1.2842827774361467
1101	80-81	1.2728173307496569
1101	82-83	1.3193793236599305
1101	84-85	1.302036434084961
1101	86-87	1.3238411740385736
1101	88-89	1.33733477466145
1101	90-91	1.3914605651435004
1101	92-93	1.4237717268751062
1101	94-95	1.3122254792219152
1101	96-97	1.3668864152211277
1101	98-99	1.3441224217828527
1101	100	1.4708636561340214
1102	1	0.405265553534754
1102	2	0.5062315793847958
1102	3	0.5381637592928854
1102	4	0.40954451131415226
1102	5	0.4708428761991712
1102	6	0.3904444454647802
1102	7	0.42271347982875795
1102	8	0.43607876912211907
1102	9	0.5166673164771183
1102	10-11	0.5212531608072339
1102	12-13	0.6256131997252936
1102	14-15	0.761307745714646
1102	16-17	0.7485919182184517
1102	18-19	0.7109211470569292
1102	20-21	0.7216109117445981
1102	22-23	0.7367184428890141
1102	24-25	0.7118775379408788
1102	26-27	0.7378118903082154
1102	28-29	0.7421407494684402
1102	30-31	0.8479666479702601
1102	32-33	0.9033467315431665
1102	34-35	0.8448262392873787
1102	36-37	0.8437616707611326
1102	38-39	0.885317626742129
1102	40-41	0.9622784323224565
1102	42-43	0.9028181984828052
1102	44-45	1.0164011210596016
1102	46-47	0.9656523022890084
1102	48-49	1.1185810411734636
1102	50-51	1.1398988284996463
1102	52-53	1.161489703710906
1102	54-55	1.2675956660235173
1102	56-57	1.291166926023024
1102	58-59	1.2724181728753479
1102	60-61	1.2504040099468696
1102	62-63	1.321549033671623
1102	64-65	1.2570865615095173
1102	66-67	1.2193975292919816
1102	68-69	1.1940115285718633
1102	70-71	1.1195264286891629
1102	72-73	1.0498173139149571
1102	74-75	1.0077710562774413
1102	76-77	0.9702039467333208
1102	78-79	1.0479054017576033
1102	80-81	0.9805094571501733
1102	82-83	0.9556877321274229
1102	84-85	1.050348796507162
1102	86-87	1.0485383943374629
1102	88-89	1.0565697422282518
1102	90-91	1.099912161945305
1102	92-93	1.0868507258383637
1102	94-95	0.9843916773002128
1102	96-97	1.0699376437181805
1102	98-99	1.1422184057824616
1102	100	1.1297301229611634
1103	1	0.4164721245019152
1103	2	0.47553682334282854
1103	3	0.530599303291929
1103	4	0.41801127969284835
1103	5	0.4419708026036204
1103	6	0.4593007326561249
1103	7	0.45541870457103073
1103	8	0.4453162049629569
1103	9	0.5474385373422663
1103	10-11	0.5299086080252877
1103	12-13	0.5523560426887997
1103	14-15	0.675012844259065
1103	16-17	0.709181957504569
1103	18-19	0.672469949030976
1103	20-21	0.6792608431047782
1103	22-23	0.7091816657350734
1103	24-25	0.6932424700515085
1103	26-27	0.6944109967980978
1103	28-29	0.7177433213413735
1103	30-31	0.7733967608841468
1103	32-33	0.7599512370308972
1103	34-35	0.8212886112934896
1103	36-37	0.7899030282884425
1103	38-39	0.8102556275254997
1103	40-41	0.8191773994728848
1103	42-43	0.8735711588798907
1103	44-45	0.924640197747209
1103	46-47	0.9221692565840982
1103	48-49	0.9459371681800519
1103	50-51	0.8568335828351721
1103	52-53	0.8628276023825023
1103	54-55	0.9669072812109647
1103	56-57	0.9119419470136663
1103	58-59	0.9320034973418458
1103	60-61	0.9047425681704695
1103	62-63	0.928117275013193
1103	64-65	0.9457840307367178
1103	66-67	0.9146382566218989
1103	68-69	0.8395192923032937
1103	70-71	0.8929860012267667
1103	72-73	0.8620486714317437
1103	74-75	0.9104905903093581
1103	76-77	0.9068400126705711
1103	78-79	0.8600485878492705
1103	80-81	0.9377882331564003
1103	82-83	0.8937972278317297
1103	84-85	0.8658152042355418
1103	86-87	0.8696655792093573
1103	88-89	0.8469732650205621
1103	90-91	0.8629799567423753
1103	92-93	0.9328654254854101
1103	94-95	0.9726774082867564
1103	96-97	0.9488836668767888
1103	98-99	0.9590495652734354
1103	100	1.0082667469108237
1104	1	0.3097870399510043
1104	2	0.37964324249885806
1104	3	0.383368114200735
1104	4	0.3394300021485961
1104	5	0.3556350860034527
1104	6	0.3193835658771462
1104	7	0.3544657393722588
1104	8	0.31717025067891313
1104	9	0.4475890482010456
1104	10-11	0.43177808044733723
1104	12-13	0.4226658289460232
1104	14-15	0.5226656527871185
1104	16-17	0.48216240351854367
1104	18-19	0.49492563791428523
1104	20-21	0.468329231426587
1104	22-23	0.42531212401475216
1104	24-25	0.4255425639626793
1104	26-27	0.44621804272861
1104	28-29	0.48343953596342715
1104	30-31	0.46303645044829267
1104	32-33	0.47566610942624976
1104	34-35	0.46760590661443047
1104	36-37	0.4992781178521781
1104	38-39	0.4748145297155162
1104	40-41	0.4674109282665668
1104	42-43	0.5033110648962023
1104	44-45	0.5389325287454625
1104	46-47	0.5267522123196358
1104	48-49	0.4206056787536099
1104	50-51	0.31761967732578
1104	52-53	0.32531014974104977
1104	54-55	0.4861198785679406
1104	56-57	0.44036412474765285
1104	58-59	0.46912139517881
1104	60-61	0.4028600773708746
1104	62-63	0.40919938879112294
1104	64-65	0.4059143990631
1104	66-67	0.37657449451938163
1104	68-69	0.4134916038377412
1104	70-71	0.37103552153795505
1104	72-73	0.37038100341671054
1104	74-75	0.3891313967325729
1104	76-77	0.3706752205844168
1104	78-79	0.4032755900379499
1104	80-81	0.4824771903843583
1104	82-83	0.4407187522737601
1104	84-85	0.43105870482939324
1104	86-87	0.36769326751195663
1104	88-89	0.42412704314377336
1104	90-91	0.3669792732113528
1104	92-93	0.41993344670159516
1104	94-95	0.3822753481931862
1104	96-97	0.4404044580160793
1104	98-99	0.46019491157826664
1104	100	0.5090690444028922
1105	1	0.29722933366277005
1105	2	0.33497806555650556
1105	3	0.37549251804394856
1105	4	0.2986712487950811
1105	5	0.3609808454171173
1105	6	0.33937535743296365
1105	7	0.3697091499089069
1105	8	0.3504191331920623
1105	9	0.442078109429481
1105	10-11	0.4081128828898457
1105	12-13	0.4434172925018842
1105	14-15	0.5356556410888587
1105	16-17	0.5122819780321635
1105	18-19	0.5484474285160488
1105	20-21	0.5387472310864894
1105	22-23	0.4288892675875502
1105	24-25	0.5372164777092578
1105	26-27	0.4666614222577081
1105	28-29	0.4619609826720321
1105	30-31	0.4825387612444487
1105	32-33	0.4780300384586482
1105	34-35	0.4999792905885059
1105	36-37	0.48768470461047997
1105	38-39	0.4685255902676744
1105	40-41	0.5681196930137347
1105	42-43	0.5694471243761186
1105	44-45	0.5864061604369368
1105	46-47	0.5236637934947055
1105	48-49	0.5131989057951287
1105	50-51	0.46527715264688396
1105	52-53	0.45314996515025996
1105	54-55	0.4840706717146688
1105	56-57	0.5347150976555497
1105	58-59	0.4630427929642238
1105	60-61	0.4205723919437716
1105	62-63	0.5132059968505231
1105	64-65	0.48407056668663273
1105	66-67	0.4254279675951622
1105	68-69	0.4021005620464706
1105	70-71	0.41778836973863775
1105	72-73	0.4195324834351908
1105	74-75	0.45123166240986023
1105	76-77	0.40297605508499146
1105	78-79	0.4321632894273044
1105	80-81	0.4115887208955762
1105	82-83	0.4335057082601139
1105	84-85	0.4659117337390235
1105	86-87	0.40592521738918563
1105	88-89	0.41535182060801645
1105	90-91	0.47789444614315
1105	92-93	0.5134252122026659
1105	94-95	0.5483742008291124
1105	96-97	0.6044442848650391
1105	98-99	0.5742670514777721
1105	100	0.6071710159378334
1106	1	0.13899236352147426
1106	2	0.21768434388228997
1106	3	0.24486901764404934
1106	4	0.19530298033333793
1106	5	0.26139906722463735
1106	6	0.24949932437511535
1106	7	0.25845964977560953
1106	8	0.22489843871058923
1106	9	0.26404641788055727
1106	10-11	0.30040202245262293
1106	12-13	0.33532320092630385
1106	14-15	0.36702144354153887
1106	16-17	0.370059487363001
1106	18-19	0.3538993080148529
1106	20-21	0.3313712813397487
1106	22-23	0.3574270741724632
1106	24-25	0.4070865956777965
1106	26-27	0.43290375763493927
1106	28-29	0.3688774716083145
1106	30-31	0.3962742651100868
1106	32-33	0.38763747647518443
1106	34-35	0.4412116286316987
1106	36-37	0.4622289928002985
1106	38-39	0.5151527396944928
1106	40-41	0.5053135246586251
1106	42-43	0.47473696375227803
1106	44-45	0.4778871821644799
1106	46-47	0.45604015979702694
1106	48-49	0.49102565199613224
1106	50-51	0.43250672476098373
1106	52-53	0.4651426004475141
1106	54-55	0.5141459849644718
1106	56-57	0.5079784774209415
1106	58-59	0.4848557261820261
1106	60-61	0.4812270507014347
1106	62-63	0.5273668039686257
1106	64-65	0.4990540710854532
1106	66-67	0.4474741719406694
1106	68-69	0.3917095829742294
1106	70-71	0.35630476536647393
1106	72-73	0.31996111913232994
1106	74-75	0.4087617377231112
1106	76-77	0.40734155761764157
1106	78-79	0.3618257960921909
1106	80-81	0.4226524372378826
1106	82-83	0.4270303516885363
1106	84-85	0.39974187903361624
1106	86-87	0.4909337984342379
1106	88-89	0.44399834820065465
1106	90-91	0.4476775039943881
1106	92-93	0.47008676912082237
1106	94-95	0.3916222513489771
1106	96-97	0.4000446247743987
1106	98-99	0.43839078514879404
1106	100	0.5308247082865414
1107	1	0.2065406184742571
1107	2	0.2917978287588241
1107	3	0.2919851872086099
1107	4	0.28918579454283133
1107	5	0.33524850167280107
1107	6	0.26564216829290643
1107	7	0.3064355612229832
1107	8	0.24148864296569172
1107	9	0.3474881942351047
1107	10-11	0.3888556284767688
1107	12-13	0.3754618905208247
1107	14-15	0.41652327190664806
1107	16-17	0.40390909539013364
1107	18-19	0.45041815553467046
1107	20-21	0.40923972636677064
1107	22-23	0.4054856986275297
1107	24-25	0.4730775053999068
1107	26-27	0.4352570676280365
1107	28-29	0.42258227795730363
1107	30-31	0.3934641268360295
1107	32-33	0.43098750845579303
1107	34-35	0.35406814930968267
1107	36-37	0.36946549992860866
1107	38-39	0.46645871102135317
1107	40-41	0.4381109811481636
1107	42-43	0.43672014552837624
1107	44-45	0.3717540323689903
1107	46-47	0.3514072845719056
1107	48-49	0.38916346527987145
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2316	82-83	-0.626730149156753
2316	84-85	-0.6352514174414701
2316	86-87	-0.6903415055423423
2316	88-89	-0.6213747312325602
2316	90-91	-0.5839443246941087
2316	92-93	-0.5069390978500117
2316	94-95	-0.48022109016989134
2316	96-97	-0.5174974836311179
2316	98-99	-0.5963217567973054
2316	100	-0.7060896800939886
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20140.0
3	3448.0
4	3211.0
5	3771.0
6	4443.0
7	5203.0
8	6351.0
9	7717.0
10	10272.0
11	15246.0
12	23849.0
13	34428.0
14	36949.0
15	46401.0
16	63241.0
17	88640.0
18	88678.0
19	50100.0
20	40781.0
21	36672.0
22	37831.0
23	43480.0
24	48169.0
25	53067.0
26	59048.0
27	68138.0
28	80272.0
29	95063.0
30	116901.0
31	145555.0
32	187716.0
33	252745.0
34	365963.0
35	592668.0
36	1078708.0
37	1904942.0
38	1680996.0
39	350319.0
40	444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60065792541041	18.106677629076632	13.330900112271712	23.96176433324125
2	15.706336429009255	21.605552898353878	37.08008264918662	25.60802802345024
3	18.945655963024755	26.33671641141641	29.567958275945838	25.149669349612996
4	13.577389991211547	17.5995803503805	34.90392460706083	33.919105051347124
5	14.131000330090698	36.26206121778494	33.14884399028542	16.45809446183894
6	31.343592849273787	34.6197042853511	17.792304226237103	16.244398639138012
7	26.128892219437194	32.07465309181116	21.853678389622473	19.942776299129175
8	27.288458910364565	31.12661916855835	20.96443686996095	20.620485051116137
9	27.57531894682942	14.373688601062861	19.85295914168596	38.198033310421756
10-11	25.201378569475626	25.267592305917287	26.41638512039537	23.11464400421172
12-13	27.021596479525016	23.461397012835114	26.56553095151486	22.951475556125008
14-15	25.161717222268052	23.23078112704864	25.314605283362297	26.29289636732101
16-17	23.0550925874493	26.307124530138687	26.6433099938846	23.99447288852741
18-19	23.870813904984026	25.15326746090088	28.885836212498578	22.090082421616515
20-21	24.570529597740563	24.49189524110326	28.054544541886074	22.88303061927011
22-23	23.667265195618953	25.041510670535917	26.930210645493837	24.3610134883513
24-25	23.294492755458663	25.82321513760955	26.636409919180004	24.24588218775178
26-27	23.823351026392793	24.981722958232332	26.922594571697594	24.27233144367728
28-29	23.250570066465592	24.519271247992112	28.56741678489944	23.662741900642853
30-31	24.29932520369718	24.58878034707079	27.843429571226668	23.26846487800536
32-33	22.646905071154116	25.424639933972298	27.751892695162102	24.176562299711485
34-35	22.698665575389636	25.72180522125939	28.033141081012726	23.546388122338254
36-37	23.573392898554392	25.61323658762456	27.11985464901575	23.6935158648053
38-39	24.004813907808835	24.75296831179724	27.04091085684435	24.20130692354958
40-41	23.445880639232925	25.440352540258054	27.07958314084748	24.03418367966154
42-43	24.602924443600408	25.093380655020475	26.690210027964007	23.61348487341511
44-45	24.324006560057448	25.038592086312494	26.768235515385534	23.86916583824452
46-47	23.603738183012375	25.185780993304242	27.53566194735391	23.674818876329475
48-49	24.147459890723283	25.693953177889718	26.78166500665889	23.376921924728112
50-51	23.299062574956782	26.141100717103793	25.92830099335831	24.63153571458111
52-53	23.12313419583558	26.255457366193223	25.102399271597804	25.51900916637339
54-55	23.665455977100702	25.876671017834408	25.059486217985437	25.398386787079453
56-57	24.06444561883698	25.02326891624591	25.325830991917798	25.58645447299931
58-59	23.754595699814594	24.894764479586236	26.551986618293327	24.798653202305847
60-61	23.815889827143806	25.227950496533698	26.066679466333927	24.88948020998857
62-63	22.65255947135142	25.37105797508482	26.45116553841386	25.525217015149902
64-65	22.623703406778432	25.29327588518049	26.548738164684416	25.534282543356664
66-67	23.78583409022277	25.058628665490122	25.92186679895202	25.233670445335083
68-69	23.87003318954297	24.406212683714916	25.951281201062727	25.77247292567939
70-71	23.141072373343032	24.835291433992055	26.12140422141667	25.90223197124824
72-73	24.10357682958105	24.500200582561526	26.02785804310232	25.368364544755106
74-75	24.261805371859673	24.190559554336215	26.229027513332902	25.31860756047121
76-77	23.44964377741289	24.83398694179036	26.77125565270981	24.945113628086933
78-79	23.645698011020823	24.750473973314076	26.58011723696896	25.023710778696145
80-81	23.164222247028217	24.730718507718564	26.388472556391147	25.716586688862076
82-83	23.210868812353443	25.025541070907494	25.633715014481133	26.129875102257937
84-85	23.54430538399929	24.864764777705396	25.414330588799196	26.176599249496117
86-87	23.713093036997503	24.063228418930922	25.806164602850046	26.417513941221525
88-89	23.840205477898856	23.59638912234963	26.403633489729238	26.159771910022272
90-91	24.307898950535993	24.017228044320884	25.84469002825353	25.83018297688959
92-93	23.762643392506615	24.10511440860232	26.10354749119529	26.02869470769578
94-95	23.392075496902706	24.206357527633322	26.399742617134837	26.001824358329138
96-97	24.47164959212882	23.917580100020782	25.607133812180233	26.00363649567016
98-99	24.60690766151772	23.510206486543183	25.540166491956086	26.342719359983008
100	24.653726160328155	23.69978964576471	25.794474432973352	25.852009760933786
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2120.0
1	2394.5
2	1889.5
3	1052.0
4	910.0
5	955.0
6	1253.0
7	2138.0
8	3286.5
9	3535.5
10	2910.5
11	2553.5
12	2830.5
13	3141.0
14	3481.5
15	4004.0
16	4630.5
17	5276.0
18	6042.5
19	6965.5
20	8051.5
21	9470.0
22	11348.0
23	13700.5
24	16401.5
25	20025.0
26	24968.5
27	30398.5
28	36070.0
29	44035.0
30	53188.0
31	61557.0
32	70306.5
33	79783.5
34	89277.5
35	96269.5
36	103594.0
37	114122.0
38	123271.5
39	133355.0
40	141883.5
41	148895.5
42	159444.0
43	178231.0
44	201856.0
45	224386.0
46	252998.0
47	284989.0
48	352244.0
49	429682.5
50	513052.5
51	494441.0
52	387695.5
53	352315.0
54	327258.0
55	292498.5
56	266998.5
57	239739.0
58	222241.5
59	203694.0
60	170362.0
61	138770.0
62	112590.5
63	89469.0
64	69781.5
65	56471.0
66	45389.5
67	35343.5
68	28751.5
69	24259.0
70	20277.5
71	16723.0
72	13634.0
73	12369.0
74	10247.0
75	8304.5
76	6604.5
77	4860.5
78	3596.5
79	2596.0
80	1853.0
81	1338.0
82	1008.5
83	758.5
84	547.0
85	382.5
86	272.0
87	188.0
88	143.0
89	118.0
90	99.0
91	84.5
92	66.5
93	51.5
94	48.0
95	48.0
96	39.5
97	30.0
98	22.5
99	12.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11279011234633105
2	0.36534553147067317
3	0.3768270824243772
4	0.3732536109477749
5	0.3017067777014348
6	0.13661755573002926
7	0.15178868373177756
8	0.12621965677645008
9	0.20678402273811514
10-11	0.2065518115952312
12-13	0.21984589952533462
14-15	0.1495117244696104
16-17	0.13483727030125267
18-19	0.15363992256532422
20-21	0.15411724547014113
22-23	0.16244459506633885
24-25	0.17502914894874144
26-27	0.16812731775746992
28-29	0.17612570156791543
30-31	0.21752378809649558
32-33	0.21454374509615215
34-35	0.13128960006274862
36-37	0.09033013458183804
38-39	0.08394432815253072
40-41	0.08266716686666926
42-43	0.07374638879421268
44-45	0.09580644736818342
46-47	0.14277760132597725
48-49	0.2214326756683746
50-51	0.17769312678238178
52-53	0.110829218250867
54-55	0.059387999792558044
56-57	0.07375928941326178
58-59	0.10911343591733594
60-61	0.0847957690097717
62-63	0.09405841348702959
64-65	0.10325655486904195
66-67	0.09934121698763837
68-69	0.08976250734367738
70-71	0.12526501096681625
72-73	0.14978908777916616
74-75	0.19314161809368585
76-77	0.19222567414119934
78-79	0.1540656429939447
80-81	0.0905816966532956
82-83	0.13046396044360586
84-85	0.15952260485171643
86-87	0.17123636694830438
88-89	0.15918718875643967
90-91	0.17995718542549985
92-93	0.09932186605906472
94-95	0.3368351633721496
96-97	0.33891861334858014
98-99	0.37761402018637263
100	0.37868477156744845
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7751566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.49399455284267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.08987383369046	28.15419320494033
2	12.967002759247306	9.723694616438287
3	4.350077401163626	4.893053351510189
4	2.0281593232648376	3.0417517847515554
5	1.1755193794626455	2.2037458605166713
6	0.7705140631770517	1.7333790052589428
7	0.5569189919178468	1.4616782354539666
8	0.39699681982274837	1.1907997279943994
9	0.32635102707757785	1.1012583268405185
>10	2.005587440578107	14.207912747257915
>50	0.17386964665556784	4.518042649384977
>100	0.13223379654072667	10.14309025672158
>500	0.016814128284684496	4.284259735667084
>1k	0.008838478130412717	5.908318582250849
>5k	6.559807984038566E-4	1.7640684337902062
>10k+	5.869301880455558E-4	5.670753481222586
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	64450	0.8314448977148617	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	59817	0.771676329660355	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	30049	0.3876507018065769	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29790	0.3843094414728585	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	27272	0.3518256827072104	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	24460	0.31554914194112516	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	23222	0.2995781755583323	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	23132	0.29841711984391284	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	20876	0.2693133232691304	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	20871	0.26924882017388485	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	20217	0.26081181531576975	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20003	0.25805108283926115	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	19972	0.25765116364873886	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	16075	0.20737745121437398	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14308	0.18458205735460423	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	12745	0.16441838978085202	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	10809	0.13944279130178341	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	9987	0.12883848244341853	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	9633	0.12427166330003511	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	9001	0.11611847206100032	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	8981	0.11586045968001821	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	8558	0.11040349782224648	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	7957	0.10265022577373398	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1610557144195121E-4	0.0	0.0	0.0	0.0
2	2.1931052383479673E-4	0.0	0.0	0.0	0.0
3	2.451117619330081E-4	0.0	0.0	0.0	0.0
4	3.096148571785366E-4	0.0	0.0	1.290061904910569E-5	0.0
5	0.0013287637620578861	0.0	0.0	7.740371429463415E-5	0.0
6	0.0028123349527050404	0.0	0.0	7.740371429463415E-5	1.290061904910569E-5
7	0.003238055381325528	0.0	0.0	7.740371429463415E-5	1.290061904910569E-5
8	0.0035734714766022762	0.0	0.0	9.030433334373983E-5	1.290061904910569E-5
9	0.003908887571879024	0.0	0.0	1.1610557144195121E-4	1.290061904910569E-5
10-11	0.006559964786470243	0.0	0.0	1.7415835716292683E-4	1.290061904910569E-5
12-13	0.013435994739643575	0.0	0.0	2.451117619330081E-4	1.9350928573658533E-5
14-15	0.021595636288202928	6.450309524552845E-6	0.0	2.90263928604878E-4	2.580123809821138E-5
16-17	0.024994949407642277	1.290061904910569E-5	0.0	4.3862104766959347E-4	2.580123809821138E-5
18-19	0.026846188241188944	1.290061904910569E-5	0.0	5.224750714887805E-4	3.225154762276423E-5
20-21	0.03399958150391805	1.290061904910569E-5	6.450309524552845E-6	8.449905477164228E-4	5.160247619642276E-5
22-23	0.036728062432803896	1.290061904910569E-5	1.290061904910569E-5	0.002399515143133658	7.740371429463413E-5
24-25	0.054053593815752844	1.290061904910569E-5	1.290061904910569E-5	0.006269700857865365	9.030433334373983E-5
26-27	0.11008743265554341	1.290061904910569E-5	1.290061904910569E-5	0.011758914263259836	9.030433334373983E-5
28-29	0.12223336549027641	1.290061904910569E-5	1.290061904910569E-5	0.03488972421830634	9.030433334373983E-5
30-31	0.12392334658570926	1.290061904910569E-5	1.290061904910569E-5	0.1037016262262361	9.030433334373983E-5
32-33	0.12530371282396358	1.290061904910569E-5	1.290061904910569E-5	0.2139374160008442	9.030433334373983E-5
34-35	0.12636801389551477	1.290061904910569E-5	1.290061904910569E-5	0.35855980585084357	9.030433334373983E-5
36-37	0.1281741005623896	1.290061904910569E-5	1.290061904910569E-5	0.5770317894474484	9.030433334373983E-5
38-39	0.13009629280070634	1.290061904910569E-5	1.290061904910569E-5	0.8769002289343857	9.030433334373983E-5
40-41	0.13195398194377755	1.290061904910569E-5	1.290061904910569E-5	1.2643251699076032	9.030433334373983E-5
42-43	0.13570161177754275	1.290061904910569E-5	1.290061904910569E-5	1.566618925775772	9.030433334373983E-5
44-45	0.14042968865904	1.290061904910569E-5	1.290061904910569E-5	1.888837687765285	1.0320495239284552E-4
46-47	0.1429195081355174	1.9350928573658533E-5	1.290061904910569E-5	2.26151077085585	1.290061904910569E-4
48-49	0.1447255948023922	2.580123809821138E-5	1.290061904910569E-5	2.61003389508649	1.290061904910569E-4
50-51	0.14717671242172226	2.580123809821138E-5	1.290061904910569E-5	2.9852548504392535	1.290061904910569E-4
52-53	0.14832486751709267	2.580123809821138E-5	1.9350928573658533E-5	3.399912998225133	1.290061904910569E-4
54-55	0.15076308451737364	3.8701857147317067E-5	2.580123809821138E-5	3.7852093370552478	1.3545650001560975E-4
56-57	0.15607168925608064	5.160247619642276E-5	2.580123809821138E-5	4.193584883364213	1.419068095401626E-4
58-59	0.15791647778010276	5.160247619642276E-5	2.580123809821138E-5	4.617292815413041	1.419068095401626E-4
60-61	0.15910978504214504	5.160247619642276E-5	2.580123809821138E-5	5.009800600291605	1.419068095401626E-4
62-63	0.15981931908984584	5.160247619642276E-5	2.580123809821138E-5	5.4196403668626445	1.67708047638374E-4
64-65	0.16043209849467838	5.160247619642276E-5	2.580123809821138E-5	5.870594406343183	1.67708047638374E-4
66-67	0.1612061356376247	5.160247619642276E-5	2.580123809821138E-5	6.335074744896708	1.67708047638374E-4
68-69	0.16218013237583218	5.160247619642276E-5	2.580123809821138E-5	6.862258542338413	1.67708047638374E-4
70-71	0.16332183716167803	5.160247619642276E-5	2.580123809821138E-5	7.454455009478085	1.67708047638374E-4
72-73	0.16604386778103936	5.160247619642276E-5	2.580123809821138E-5	7.974898233466631	1.8060866668747965E-4
74-75	0.1683208270432065	5.160247619642276E-5	2.580123809821138E-5	8.488510579668676	1.8060866668747965E-4
76-77	0.16908196356710373	6.450309524552845E-5	2.580123809821138E-5	9.053854408257635	1.8060866668747965E-4
78-79	0.16968184235288714	6.450309524552845E-5	2.580123809821138E-5	9.628157716776197	1.870589762120325E-4
80-81	0.17099770549589594	6.450309524552845E-5	2.580123809821138E-5	10.251418874586115	2.0640990478569104E-4
82-83	0.17159113397215478	7.09534047700813E-5	2.580123809821138E-5	10.936538500736496	2.451117619330081E-4
84-85	0.17362943178191348	7.740371429463415E-5	2.580123809821138E-5	11.597404963074558	2.451117619330081E-4
86-87	0.17735771068710504	7.740371429463415E-5	2.580123809821138E-5	12.266901939556472	2.451117619330081E-4
88	0.17814464844910047	7.740371429463415E-5	2.580123809821138E-5	12.795659612522167	2.580123809821138E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	7035	0.0	25.845589	1
ATCTACA	8185	0.0	22.785328	2
TCTACAC	9260	0.0	22.22812	3
TACACTC	9100	0.0	21.251442	5
AGTACTT	33275	0.0	21.156137	12-13
GAGTACT	32080	0.0	20.508133	12-13
CTACACT	11235	0.0	19.864351	4
GTGATCG	1645	0.0	19.15443	8
TATACCG	1340	0.0	18.2594	5
TACTTTT	39030	0.0	18.252161	14-15
GTATAAG	2505	0.0	18.240099	1
GTACTTT	36720	0.0	17.967577	14-15
CACTCTT	11810	0.0	16.567614	7
CATGGGG	15465	0.0	16.37953	4
ACTTTTT	41035	0.0	16.342932	16-17
ATAAGGT	2485	0.0	15.921544	3
TATAAGG	2875	0.0	15.72578	2
ACACTCT	12525	0.0	15.619614	6
TGATCGC	2005	0.0	15.480675	9
ACTCTTT	14480	0.0	15.427139	8
>>END_MODULE