##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765295_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7751566 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.482958927267084 34.0 31.0 34.0 31.0 34.0 2 32.62367720277425 34.0 31.0 34.0 31.0 34.0 3 32.661465954105275 34.0 31.0 34.0 31.0 34.0 4 36.181597499137595 37.0 35.0 37.0 35.0 37.0 5 36.1196200870895 37.0 35.0 37.0 35.0 37.0 6 36.171036149340665 37.0 37.0 37.0 35.0 37.0 7 36.119983884546684 37.0 36.0 37.0 35.0 37.0 8 36.1267141375046 37.0 36.0 37.0 35.0 37.0 9 37.85074422381232 39.0 38.0 39.0 35.0 39.0 10-11 37.802320395646504 39.0 38.0 39.0 35.0 39.0 12-13 37.76516996694603 39.0 38.0 39.0 35.0 39.0 14-15 39.12343983138375 40.0 38.5 41.0 36.0 41.0 16-17 39.11159164483667 40.0 38.5 41.0 36.0 41.0 18-19 39.154531548850905 40.0 39.0 41.0 36.0 41.0 20-21 39.130816921380784 40.0 39.0 41.0 36.0 41.0 22-23 39.04966989895978 40.0 39.0 41.0 35.0 41.0 24-25 38.98021528811081 40.0 38.0 41.0 35.0 41.0 26-27 38.84408976199132 40.0 38.0 41.0 35.0 41.0 28-29 38.70556110855536 40.0 38.0 41.0 35.0 41.0 30-31 38.51975684655204 40.0 38.0 41.0 34.5 41.0 32-33 38.27290846778574 40.0 38.0 41.0 34.0 41.0 34-35 38.144199443054475 40.0 38.0 41.0 34.0 41.0 36-37 37.99429695883387 40.0 37.5 41.0 33.5 41.0 38-39 37.79977155583788 40.0 37.0 41.0 33.0 41.0 40-41 37.561197053601816 40.0 37.0 41.0 33.0 41.0 42-43 37.274095969769206 40.0 36.5 41.0 32.5 41.0 44-45 36.92068325290657 40.0 36.0 41.0 31.5 41.0 46-47 36.53133044342266 39.0 35.5 41.0 31.0 41.0 48-49 36.16738637070239 39.0 35.0 41.0 30.0 41.0 50-51 35.83519794580863 39.0 35.0 41.0 29.5 41.0 52-53 35.66382418726745 39.0 35.0 40.5 29.0 41.0 54-55 35.630416744177886 39.0 35.0 41.0 29.0 41.0 56-57 35.445089676073195 39.0 35.0 41.0 29.0 41.0 58-59 35.285092457446666 38.0 35.0 41.0 28.5 41.0 60-61 34.97747068140812 37.5 35.0 40.0 28.0 41.0 62-63 34.64986816857393 37.0 35.0 40.0 28.0 41.0 64-65 34.389082735024125 36.5 35.0 39.5 28.0 41.0 66-67 34.040367327066555 36.0 34.0 39.0 27.5 41.0 68-69 33.69290482723104 35.5 34.0 39.0 26.5 41.0 70-71 33.33134156375628 35.0 34.0 37.5 26.0 40.0 72-73 32.97422669174203 35.0 34.0 37.0 26.0 39.0 74-75 32.63615390748141 35.0 34.0 37.0 26.0 39.0 76-77 31.22363358578125 34.0 31.5 35.0 23.5 37.0 78-79 32.00113370640204 35.0 33.0 36.0 25.0 37.0 80-81 31.960045234730636 35.0 33.5 35.0 25.0 37.0 82-83 31.793575517514782 35.0 33.5 35.0 25.0 36.5 84-85 31.615118029053743 35.0 33.0 35.0 24.0 36.0 86-87 31.448464607022633 35.0 33.0 35.0 24.0 36.0 88-89 31.30114044052518 35.0 33.0 35.0 23.5 36.0 90-91 31.17291396344945 35.0 33.0 35.0 22.5 35.0 92-93 31.02798047517108 35.0 33.0 35.0 20.0 35.0 94-95 30.915716708081952 35.0 33.0 35.0 19.0 35.0 96-97 30.79149916287883 35.0 33.0 35.0 16.5 35.0 98-99 30.65191717389751 35.0 33.0 35.0 2.0 35.0 100 30.318709019571013 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38923236590207466 1101 2 0.32141571628443444 1101 3 0.3260593657432622 1101 4 0.24267833068790168 1101 5 0.2776981509265468 1101 6 0.2762353567023865 1101 7 0.2646724160094891 1101 8 0.2765007544323126 1101 9 0.35071836804898027 1101 10-11 0.3861849781629729 1101 12-13 0.39646851586616094 1101 14-15 0.5248138700375407 1101 16-17 0.4490981397169591 1101 18-19 0.48163015118071684 1101 20-21 0.43576607183202754 1101 22-23 0.46153437444645107 1101 24-25 0.49150046490483845 1101 26-27 0.5206168692944928 1101 28-29 0.48717660200369295 1101 30-31 0.4877681262082234 1101 32-33 0.5652597167329176 1101 34-35 0.4930249248753853 1101 36-37 0.6111174705567564 1101 38-39 0.6298316828487316 1101 40-41 0.6607899675911 1101 42-43 0.7095862286725563 1101 44-45 0.764127847446133 1101 46-47 0.7868757477637871 1101 48-49 0.8664576042046548 1101 50-51 0.9372215627605911 1101 52-53 0.9484902717536912 1101 54-55 1.023025319197238 1101 56-57 1.0190374178744293 1101 58-59 1.0065868775322357 1101 60-61 1.0556319115799582 1101 62-63 1.051225377598854 1101 64-65 1.0199929287689216 1101 66-67 1.0437890290294547 1101 68-69 1.0345332311025928 1101 70-71 1.0479654555488338 1101 72-73 1.0372781924209917 1101 74-75 1.0544920189132867 1101 76-77 1.008530358273049 1101 78-79 1.025350977431664 1101 80-81 1.0778338474291296 1101 82-83 1.1033784888336449 1101 84-85 1.051422090235171 1101 86-87 1.0398355237162882 1101 88-89 1.1468199147569607 1101 90-91 1.1091298225193498 1101 92-93 1.2235123519337634 1101 94-95 1.2071308595533594 1101 96-97 1.2328915149676263 1101 98-99 1.308036045599355 1101 100 1.2577394446889159 1102 1 0.37683498873999355 1102 2 0.30770305005918885 1102 3 0.3965049063308399 1102 4 0.26799259665474295 1102 5 0.24894033722682707 1102 6 0.28286635557832085 1102 7 0.2982181915073241 1102 8 0.2848561072430087 1102 9 0.3603617951636622 1102 10-11 0.36442985443257214 1102 12-13 0.406983708375229 1102 14-15 0.4940676547372931 1102 16-17 0.509715989175433 1102 18-19 0.4912725487489027 1102 20-21 0.4720162923162263 1102 22-23 0.5331490333031184 1102 24-25 0.49205268635606814 1102 26-27 0.5569134193710141 1102 28-29 0.5949345449549099 1102 30-31 0.6443428349396001 1102 32-33 0.5937247174858484 1102 34-35 0.6621786326940509 1102 36-37 0.665597994886177 1102 38-39 0.5859514312622949 1102 40-41 0.745546303705531 1102 42-43 0.7906233838892192 1102 44-45 0.7625184429978518 1102 46-47 0.8068329980162758 1102 48-49 0.8599049128832874 1102 50-51 0.8387050772403555 1102 52-53 0.8511276845802485 1102 54-55 0.958054162624542 1102 56-57 1.0101234306541969 1102 58-59 0.975022619318004 1102 60-61 1.0215993590563457 1102 62-63 0.9747240569263766 1102 64-65 1.06300011252263 1102 66-67 1.0164480945832821 1102 68-69 0.9996531261335093 1102 70-71 1.0347494772849188 1102 72-73 1.0452633674476175 1102 74-75 1.019561912716192 1102 76-77 0.9826382066176222 1102 78-79 1.0271664896459285 1102 80-81 1.0029226055008316 1102 82-83 1.04005388721232 1102 84-85 1.061719118120493 1102 86-87 1.0833737922076274 1102 88-89 1.0302144089407648 1102 90-91 1.0729816015632991 1102 92-93 1.0898717713290864 1102 94-95 1.1151440308021705 1102 96-97 1.1234543692773684 1102 98-99 1.1896945135200525 1102 100 1.215116467840815 1103 1 0.35194123730985893 1103 2 0.31478445710531133 1103 3 0.348080083977095 1103 4 0.24861001130880567 1103 5 0.28212220261789156 1103 6 0.2625679317515477 1103 7 0.2706857971769665 1103 8 0.2753618356506706 1103 9 0.29120878051569576 1103 10-11 0.35004811658510704 1103 12-13 0.3346131519232429 1103 14-15 0.4544536695797561 1103 16-17 0.4546733206139777 1103 18-19 0.4529201301832515 1103 20-21 0.4443617167651084 1103 22-23 0.4533037142479799 1103 24-25 0.4660581367119079 1103 26-27 0.46284621537398607 1103 28-29 0.4643174072312135 1103 30-31 0.5352032379112757 1103 32-33 0.5109362923330139 1103 34-35 0.4781533335257251 1103 36-37 0.5135131463508387 1103 38-39 0.5095822634902376 1103 40-41 0.5108617317435318 1103 42-43 0.5397454001876483 1103 44-45 0.5429064242747899 1103 46-47 0.5426419530472231 1103 48-49 0.5022406337836145 1103 50-51 0.6877900878600727 1103 52-53 0.684131817891874 1103 54-55 0.767261429076008 1103 56-57 0.6700483152371746 1103 58-59 0.6898603241612307 1103 60-61 0.7366613431676754 1103 62-63 0.7224843310773679 1103 64-65 0.7162651650499114 1103 66-67 0.6729675824479457 1103 68-69 0.7010735431888051 1103 70-71 0.6244279300012607 1103 72-73 0.6977024923165303 1103 74-75 0.7657493482526334 1103 76-77 0.7569792759817204 1103 78-79 0.8068174928410698 1103 80-81 0.7919891840014124 1103 82-83 0.7845267575800214 1103 84-85 0.8025084712875952 1103 86-87 0.7917372884457485 1103 88-89 0.841396841192271 1103 90-91 0.8532801068671034 1103 92-93 0.8316347636088608 1103 94-95 0.8988730167650942 1103 96-97 0.9080756203096207 1103 98-99 0.91472236333151 1103 100 0.9648219489610987 1104 1 0.26335279751911855 1104 2 0.1631543152612167 1104 3 0.23766517620540384 1104 4 0.19469981195896935 1104 5 0.21829182032389127 1104 6 0.18495847985946057 1104 7 0.16191528709591552 1104 8 0.15374898435486273 1104 9 0.23970770414173614 1104 10-11 0.257074453327121 1104 12-13 0.28859775014986155 1104 14-15 0.35098566831788247 1104 16-17 0.3712208151120251 1104 18-19 0.2865630073045793 1104 20-21 0.3323238200256782 1104 22-23 0.3118533488442594 1104 24-25 0.2851308909058119 1104 26-27 0.33237633505363817 1104 28-29 0.3735217708870664 1104 30-31 0.3379435911071269 1104 32-33 0.30040533543174774 1104 34-35 0.2891099716139536 1104 36-37 0.2602416806279919 1104 38-39 0.2775390029592657 1104 40-41 0.27721220589988604 1104 42-43 0.3080144910903684 1104 44-45 0.279515711150637 1104 46-47 0.22670502677069493 1104 48-49 0.19706406569803647 1104 50-51 0.19014450757683932 1104 52-53 0.25896241347867743 1104 54-55 0.2632505934511826 1104 56-57 0.1886587897165981 1104 58-59 0.31370302329479216 1104 60-61 0.3228820922778297 1104 62-63 0.3230050885860223 1104 64-65 0.28392062003018737 1104 66-67 0.3460202986883161 1104 68-69 0.41412076852971325 1104 70-71 0.3542924812280859 1104 72-73 0.34320865981108284 1104 74-75 0.32118562696113173 1104 76-77 0.21902305655522625 1104 78-79 0.3406768954635524 1104 80-81 0.30599467477866327 1104 82-83 0.29794901852489986 1104 84-85 0.322005513370037 1104 86-87 0.35430584957525824 1104 88-89 0.3549406490919438 1104 90-91 0.3301349145176786 1104 92-93 0.3915369915398941 1104 94-95 0.41828571744208176 1104 96-97 0.431513496992892 1104 98-99 0.43418419611088765 1104 100 0.4670118209485459 1105 1 0.2068108227668901 1105 2 0.16362492338493695 1105 3 0.2142446498803139 1105 4 0.1646215507241422 1105 5 0.16098658712541436 1105 6 0.14515250420208048 1105 7 0.16870515811624642 1105 8 0.16673599470769318 1105 9 0.21784624009502096 1105 10-11 0.1930842503549286 1105 12-13 0.18926611620811684 1105 14-15 0.21505957839134027 1105 16-17 0.2701883390210895 1105 18-19 0.28734860497744563 1105 20-21 0.30412650692110077 1105 22-23 0.30642772876984026 1105 24-25 0.27443136941702306 1105 26-27 0.2797671613262196 1105 28-29 0.27874158611115263 1105 30-31 0.29524382356415657 1105 32-33 0.3190002568985264 1105 34-35 0.34430784958840377 1105 36-37 0.3566963669957559 1105 38-39 0.34733013976461535 1105 40-41 0.3461536684227795 1105 42-43 0.36201990797020756 1105 44-45 0.3916082341607776 1105 46-47 0.45735172405571944 1105 48-49 0.410682150444309 1105 50-51 0.36582504450305464 1105 52-53 0.41556255872882275 1105 54-55 0.4199721615715859 1105 56-57 0.43842407571272446 1105 58-59 0.44466575748188575 1105 60-61 0.4933469406980251 1105 62-63 0.4158834427107223 1105 64-65 0.3565083735462977 1105 66-67 0.4394696168929073 1105 68-69 0.4694860385795252 1105 70-71 0.4098743947558958 1105 72-73 0.43093051562163254 1105 74-75 0.44599806688815846 1105 76-77 0.43903176433597935 1105 78-79 0.45032159979083985 1105 80-81 0.47967861220906727 1105 82-83 0.5196225103800138 1105 84-85 0.48423376078090996 1105 86-87 0.4518293245713103 1105 88-89 0.5011923481362572 1105 90-91 0.5088413333417954 1105 92-93 0.4696850458660933 1105 94-95 0.5010523953481183 1105 96-97 0.4948771137002197 1105 98-99 0.45823626887580815 1105 100 0.4986990781189391 1106 1 0.17107451911452642 1106 2 0.04423592711727764 1106 3 0.1367069932554159 1106 4 0.08969153206245295 1106 5 0.1343811819001388 1106 6 0.09641133518048406 1106 7 0.12604153508238625 1106 8 0.1740382141158463 1106 9 0.1788233128756076 1106 10-11 0.1649250927969419 1106 12-13 0.13577413073758038 1106 14-15 0.2614513739125428 1106 16-17 0.2398214368616607 1106 18-19 0.18497173880842865 1106 20-21 0.20554237935512276 1106 22-23 0.24736431234754974 1106 24-25 0.21494306629785598 1106 26-27 0.24016522184341227 1106 28-29 0.28412556704957126 1106 30-31 0.21599412348418667 1106 32-33 0.20768202509367484 1106 34-35 0.21857262898057428 1106 36-37 0.2662163283394037 1106 38-39 0.2520118079864133 1106 40-41 0.3001409384840912 1106 42-43 0.2798584843498446 1106 44-45 0.28460760099628857 1106 46-47 0.2617059795875747 1106 48-49 0.25199321749309433 1106 50-51 0.2031142673769537 1106 52-53 0.250410765873589 1106 54-55 0.23733544802853146 1106 56-57 0.2329663644357325 1106 58-59 0.19683809485859172 1106 60-61 0.18664122888784362 1106 62-63 0.08915598669899794 1106 64-65 0.21292791166946046 1106 66-67 0.2238958532298838 1106 68-69 0.21677426172002612 1106 70-71 0.1771135476484602 1106 72-73 0.1834444285781771 1106 74-75 0.19524201850105527 1106 76-77 0.122266235143762 1106 78-79 0.2014317204253402 1106 80-81 0.2611264761120289 1106 82-83 0.2355940846268858 1106 84-85 0.19860367880277252 1106 86-87 0.24013161062836375 1106 88-89 0.24477114379075005 1106 90-91 0.2635067558957829 1106 92-93 0.28180598028357906 1106 94-95 0.30697706543609726 1106 96-97 0.37856229765116467 1106 98-99 0.3222787742077209 1106 100 0.3967221053116887 1107 1 0.13371690646704337 1107 2 0.0626765883584639 1107 3 0.14333192382382975 1107 4 0.10038504841464402 1107 5 0.12033364651107092 1107 6 0.12597157023796512 1107 7 0.09388156049855922 1107 8 0.12391886542621222 1107 9 0.12651923618947336 1107 10-11 0.1616502362888852 1107 12-13 0.1877630037998017 1107 14-15 0.20986449395764595 1107 16-17 0.2495602688961327 1107 18-19 0.207505707686245 1107 20-21 0.2317650327778935 1107 22-23 0.20572192920301546 1107 24-25 0.26233880319843195 1107 26-27 0.25062756257156593 1107 28-29 0.26027095153223456 1107 30-31 0.2746193675467268 1107 32-33 0.2874577347686511 1107 34-35 0.2546682436394718 1107 36-37 0.3107553762429873 1107 38-39 0.31551764549967487 1107 40-41 0.34604258127751564 1107 42-43 0.34537875837317245 1107 44-45 0.279465192603908 1107 46-47 0.3417268038036383 1107 48-49 0.3162641174526968 1107 50-51 0.2953159955922686 1107 52-53 0.32171306048233106 1107 54-55 0.3504249064200806 1107 56-57 0.35955355998874694 1107 58-59 0.36785611445932886 1107 60-61 0.2972012998342919 1107 62-63 0.24191796995967252 1107 64-65 0.16708005205592968 1107 66-67 0.19849585891064692 1107 68-69 0.14676118543785321 1107 70-71 0.17564325489964006 1107 72-73 0.19191836964127873 1107 74-75 0.20636566879309015 1107 76-77 0.1606077249352147 1107 78-79 0.2514917629679658 1107 80-81 0.19120479178588923 1107 82-83 0.23239313067896106 1107 84-85 0.245974299352838 1107 86-87 0.3164840011789316 1107 88-89 0.33266771913033466 1107 90-91 0.3403091367680098 1107 92-93 0.347457490525791 1107 94-95 0.30736170064674795 1107 96-97 0.29944737154320933 1107 98-99 0.28126429982587453 1107 100 0.3480560859718338 1108 1 0.038999733802967285 1108 2 0.035018882053549305 1108 3 0.02703357241396276 1108 4 0.04957436532457393 1108 5 0.08023048442325376 1108 6 0.06794111408265735 1108 7 0.052367118961996084 1108 8 0.004637211802219099 1108 9 0.025506845438428627 1108 10-11 0.06304787165742454 1108 12-13 0.06679441215138837 1108 14-15 0.02105597851062413 1108 16-17 0.07720269383121803 1108 18-19 0.08357896708685786 1108 20-21 0.07993321067358039 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-0.7943918593960788 2316 54-55 -0.8498680464397594 2316 56-57 -0.8029173583152129 2316 58-59 -0.8272318056462069 2316 60-61 -0.7397411728899854 2316 62-63 -0.7263461586623663 2316 64-65 -0.8089932857907698 2316 66-67 -0.7300955580047983 2316 68-69 -0.6394465064982029 2316 70-71 -0.7039414650690361 2316 72-73 -0.7057221516290326 2316 74-75 -0.7219532575454082 2316 76-77 -0.7171668921296472 2316 78-79 -0.7380792177032163 2316 80-81 -0.7215726293986329 2316 82-83 -0.701911882666618 2316 84-85 -0.650593200403506 2316 86-87 -0.6689883923335351 2316 88-89 -0.6619603107097234 2316 90-91 -0.6446862479114088 2316 92-93 -0.6378423970793605 2316 94-95 -0.6517530398725775 2316 96-97 -0.6180650145822426 2316 98-99 -0.6271188023179128 2316 100 -0.7146352561113147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26.0 3 29.0 4 64.0 5 129.0 6 320.0 7 858.0 8 1896.0 9 3495.0 10 6139.0 11 11116.0 12 18172.0 13 25385.0 14 30402.0 15 41346.0 16 57088.0 17 76770.0 18 76311.0 19 46989.0 20 31020.0 21 26339.0 22 27082.0 23 30073.0 24 33459.0 25 38297.0 26 45897.0 27 57495.0 28 71725.0 29 90866.0 30 113289.0 31 143571.0 32 182740.0 33 240590.0 34 344356.0 35 562907.0 36 1087173.0 37 2038171.0 38 1861671.0 39 328084.0 40 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6146708935975 18.930652206276772 13.854013498691748 23.60066340143398 2 16.148094257853842 22.37608780931981 36.03597940176409 25.439838531062257 3 19.395497491078835 26.33249741339686 29.86140091303413 24.410604182490175 4 14.150254751727372 18.10929218085334 34.45129424611499 33.28915882130429 5 14.265014320977206 37.03803850731581 32.6312902450937 16.06565692661328 6 31.705980443177545 34.788454358770856 17.306709379756295 16.198855818295296 7 26.26378979421706 32.527607969796044 21.61818657030076 19.590415665686134 8 27.71967625638484 31.28566537393863 20.750194218819786 20.244464150856743 9 27.844115627732513 14.335167887366243 19.7134875714146 38.107228913486644 10-11 25.54318606850121 25.489958049070534 25.987303324177535 22.97955255825072 12-13 27.26127853534316 23.736189777282593 26.293349478062417 22.70918220931183 14-15 25.29864893202759 23.689122456979327 24.998018134344886 26.014210476648202 16-17 23.314198830050252 26.54407752689324 26.458178350479088 23.68354529257742 18-19 24.294703805656816 25.378155846186434 28.411542906297903 21.915597441858843 20-21 25.065303102831898 24.956444709115033 27.4285739350458 22.549678253007265 22-23 24.007014926037453 25.447684686725058 26.265011169580294 24.280289217657195 24-25 23.749702880375203 26.15545427776468 26.00812809637351 24.086714745486603 26-27 24.18131717753734 25.39227298795469 26.40597075038752 24.020439084120454 28-29 23.69217012465825 24.785568957076894 28.088336294291246 23.433924623973613 30-31 24.739946339884465 24.92628087820738 27.2508473006294 23.082925481278753 32-33 23.014507580180005 25.782345659820432 27.355712758813976 23.847434001185583 34-35 23.035582904714435 26.172990668709982 27.482520360392066 23.308906066183514 36-37 24.125625124818544 26.002070375211645 26.53369416966446 23.338610330305347 38-39 24.335920184248945 25.083580046165444 26.61736452382144 23.96313524576417 40-41 23.703844274058554 25.756256265328105 26.717935952825584 23.82196350778776 42-43 25.07743009553243 25.25957264328204 26.441799498964468 23.221197762221056 44-45 24.751712256421804 25.26880947084002 26.47580826030645 23.503670012431723 46-47 23.973622573107917 25.538546227871983 27.142540241598923 23.345290957421177 48-49 24.663896816680715 25.963647589524847 26.446066425571203 22.926389168223235 50-51 23.629943937408672 26.549955230815286 25.56794875987649 24.25215207189955 52-53 23.484733446359186 26.589669578065056 24.702758820796568 25.222838154779193 54-55 24.119463191333793 26.071950982075798 24.672655377976383 25.135930448614026 56-57 24.397483438222334 25.185980062766937 24.98494442502414 25.431592073986586 58-59 23.94202006862628 25.2126683122763 26.170677393883924 24.67463422521349 60-61 24.207013937327865 25.544917924309363 25.577519672183712 24.670548466179056 62-63 22.800096814163766 25.87019212657265 25.96858029460199 25.361130764661592 64-65 22.705251932917662 25.833625068321464 25.894075009145546 25.567047989615332 66-67 23.892660749057544 25.7020656761212 25.28612972314971 25.11914385167155 68-69 23.93285099764586 25.01077863129573 25.36641541038526 25.689954960673155 70-71 23.152293138886137 25.43814638302868 25.502172308174593 25.90738816991059 72-73 23.992223695629374 25.111678428050737 25.5070214179274 25.38907645839249 74-75 24.11278624043629 24.77293768068226 25.57837030148716 25.535905777394298 76-77 23.081881437806167 25.364724086096636 26.16151427160453 25.39188020449267 78-79 23.232829230320284 25.26024781402415 25.98084773212988 25.52607522352569 80-81 22.664355821778464 25.216324030524927 25.836224577072553 26.283095570624056 82-83 22.611676188109815 25.4769074848051 25.060462873338167 26.850953453746918 84-85 22.92091338974775 25.168807847189566 24.7995985326834 27.110680230379288 86-87 22.995918089323677 24.39883057729216 25.250618685348947 27.354632648035214 88-89 22.892060996507993 24.011293986880187 25.8404164788281 27.256228537783723 90-91 23.287428770394232 24.320916796028442 25.409136043951143 26.982518389626186 92-93 22.47841998044507 24.47315674039305 25.650522563442244 27.39790071571963 94-95 21.894080516218622 24.63811099982719 26.018627882841706 27.449180601112477 96-97 22.86226735955139 24.296199187930576 25.329785485594243 27.511747966923792 98-99 22.830619000083338 23.910394364183958 25.328991586990295 27.929995048742406 100 22.72602663558799 24.21513024109241 25.560003865053886 27.49883925826571 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1116.0 1 723.5 2 396.0 3 488.0 4 454.5 5 548.0 6 831.0 7 1695.5 8 2693.5 9 2810.0 10 2266.0 11 1957.5 12 2197.0 13 2473.0 14 2779.0 15 3212.0 16 3747.5 17 4414.5 18 5092.0 19 5971.0 20 7011.0 21 8050.0 22 9631.5 23 11630.0 24 13837.5 25 16757.0 26 21014.0 27 26008.5 28 31354.5 29 39161.5 30 48062.5 31 56206.0 32 65144.5 33 75469.0 34 86417.0 35 95546.0 36 104888.0 37 117705.0 38 129578.5 39 140141.5 40 147643.0 41 153723.5 42 163260.0 43 180633.5 44 202785.0 45 224832.0 46 253014.5 47 285446.0 48 352954.5 49 430487.0 50 516405.0 51 503014.0 52 400559.5 53 359430.5 54 330952.0 55 298684.5 56 269751.0 57 240926.0 58 225825.5 59 206702.0 60 170928.5 61 137907.0 62 111357.5 63 88885.0 64 69199.5 65 55443.0 66 44342.5 67 34674.0 68 27763.5 69 22739.5 70 18672.0 71 15098.0 72 12584.5 73 12050.5 74 9443.0 75 7010.5 76 5353.5 77 3866.5 78 2851.0 79 1765.5 80 1135.5 81 803.0 82 595.0 83 421.0 84 291.0 85 188.5 86 113.0 87 51.0 88 27.5 89 16.5 90 13.5 91 11.0 92 4.0 93 4.5 94 3.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.060491002721256584 3 0.026059250479193496 4 0.006321303334061788 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.2255588096650406E-4 12-13 2.1286021431024389E-4 14-15 4.0636950004682926E-4 16-17 4.708725952923577E-4 18-19 0.0 20-21 0.0027284809288858534 22-23 0.004895784929135609 24-25 7.546862143726829E-4 26-27 5.740775476852032E-4 28-29 0.005347306595854308 30-31 0.0023866145240845527 32-33 0.0013029625239596746 34-35 0.0015351736668435772 36-37 0.004050794381419187 38-39 0.004050794381419187 40-41 9.868973572565855E-4 42-43 0.002831685881278699 44-45 3.289657857521951E-4 46-47 0.002135052452626992 48-49 3.8701857147317067E-5 50-51 0.0018125369763993496 52-53 0.006927632429369756 54-55 0.01412617785877073 56-57 0.02509170405051057 58-59 0.026536573384010408 60-61 0.025562576645802925 62-63 0.008178992477133008 64-65 0.006766374691255934 66-67 1.5480742858926827E-4 68-69 0.0015222730477944715 70-71 2.3866145240845527E-4 72-73 8.901427143882927E-4 74-75 0.0050312414291512194 76-77 0.001161055714419512 78-79 0.0013932668573034144 80-81 0.0 82-83 0.0014835711906471545 84-85 1.290061904910569E-5 86-87 6.321303334061788E-4 88-89 9.675464286829267E-5 90-91 6.321303334061788E-4 92-93 8.643414762900813E-4 94-95 0.0015674252144663415 96-97 1.5480742858926827E-4 98-99 0.0 100 7.353352857990244E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7751566.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.56472951729198 #Duplication Level Percentage of deduplicated Percentage of total 1 74.33547705824395 27.180566121735527 2 13.287921362135185 9.71738500907038 3 4.465595274005152 4.898498499830873 4 2.089558999601483 3.056166385234058 5 1.245562785817495 2.277183318011069 6 0.7900083998359807 1.733186607383477 7 0.5854904339172171 1.498580954580147 8 0.42599232882917687 1.2461035424064122 9 0.3320947037207493 1.0928657714106975 >10 2.0976221310506107 14.423647666197336 >50 0.18107046025976403 4.5974076884098505 >100 0.13534630726729238 10.162220087497115 >500 0.017684555296562775 4.391477627275051 >1k 0.009195826104280993 5.896626972306463 >5k 7.073712384808927E-4 1.8092324859164277 >10k+ 6.720026765568481E-4 6.0188512627352075 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 63882 0.8241173460949698 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 59584 0.7686704854219134 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 31808 0.4103428907139538 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 28257 0.36453279247057946 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 27487 0.3545993158027681 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26019 0.33566120703868096 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 24317 0.31370435341710307 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 22057 0.2845489543661242 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 21706 0.2800208370798881 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 21699 0.2799305327465444 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 21347 0.2753895148412592 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 19902 0.2567481203153014 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19064 0.24593740155215088 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 18233 0.23521698712234404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14875 0.19189670835544714 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 13494 0.17408095344863217 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 11084 0.14299046154028747 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 10525 0.13577901549183738 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 10068 0.1298834325863961 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9563 0.12336861996659772 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 9080 0.11713762096587967 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 8539 0.11015838606031349 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 8433 0.10879092044110829 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 8212 0.10593988363125593 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7919 0.10216000224986797 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 7891 0.101798784916493 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003625073952798699 0.0 0.0 2.580123809821138E-5 0.0 2 0.005960086000686829 0.0 0.0 3.870185714731707E-5 0.0 3 0.006243899619767154 0.0 0.0 5.160247619642276E-5 0.0 4 0.007030837381762601 0.0 0.0 6.450309524552845E-5 0.0 5 0.02933600771766634 0.0 0.0 3.4831671432585367E-4 0.0 6 0.059278344530640646 0.0 0.0 6.579315715043902E-4 0.0 7 0.06536743672181854 0.0 0.0 8.256396191427642E-4 0.0 8 0.07379154096088456 0.0 0.0 9.417451905847153E-4 0.0 9 0.07988063315206244 0.0 0.0 0.001083652000124878 0.0 10-11 0.139823359563732 0.0 0.0 0.0019028413097430894 0.0 12-13 0.24988499098117722 0.0 0.0 0.003999191905222764 0.0 14-15 0.3823936995440663 1.9350928573658533E-5 0.0 0.006618017572191219 0.0 16-17 0.4444198759321665 2.580123809821138E-5 0.0 0.007804874524708943 0.0 18-19 0.47313665393547577 2.580123809821138E-5 0.0 0.008482157024786992 0.0 20-21 0.5878554088296482 2.580123809821138E-5 6.450309524552845E-6 0.010855870929822438 0.0 22-23 0.6194490248809079 2.580123809821138E-5 1.290061904910569E-5 0.013055426477694958 6.450309524552845E-6 24-25 0.7598013098256533 2.580123809821138E-5 1.290061904910569E-5 0.025046551883838697 1.290061904910569E-5 26-27 1.058695752574383 2.580123809821138E-5 1.290061904910569E-5 0.06393546800736781 1.290061904910569E-5 28-29 1.1273076949870515 2.580123809821138E-5 1.290061904910569E-5 0.09392295698701397 1.290061904910569E-5 30-31 1.1453427604177016 2.580123809821138E-5 1.290061904910569E-5 0.16327023468548163 1.290061904910569E-5 32-33 1.1616555932052957 3.225154762276423E-5 1.290061904910569E-5 0.27400914860300485 1.290061904910569E-5 34-35 1.1745046097782048 3.870185714731707E-5 1.290061904910569E-5 0.4184057776196448 1.290061904910569E-5 36-37 1.1934104669946692 3.870185714731707E-5 1.290061904910569E-5 0.6367165034781359 1.290061904910569E-5 38-39 1.2122518211158881 5.160247619642276E-5 1.290061904910569E-5 0.935533542512571 1.290061904910569E-5 40-41 1.228771063808268 5.160247619642276E-5 1.290061904910569E-5 1.323210045557246 1.290061904910569E-5 42-43 1.2607129965738535 5.160247619642276E-5 1.290061904910569E-5 1.6263552422826562 3.870185714731707E-5 44-45 1.2996470648640546 5.160247619642276E-5 1.290061904910569E-5 1.9499930723675707 7.09534047700813E-5 46-47 1.3171790061517892 5.160247619642276E-5 1.290061904910569E-5 2.3241368260297337 7.740371429463415E-5 48-49 1.3291765818674577 5.8052785720975607E-5 1.290061904910569E-5 2.671505344855478 9.030433334373983E-5 50-51 1.3494112028459797 6.450309524552845E-5 1.290061904910569E-5 3.0439720180412575 9.030433334373983E-5 52-53 1.355655102465747 9.030433334373983E-5 1.290061904910569E-5 3.457681970327028 9.030433334373983E-5 54-55 1.373380553039218 9.030433334373983E-5 1.290061904910569E-5 3.8428622035857014 9.030433334373983E-5 56-57 1.4112761214959662 9.030433334373983E-5 1.290061904910569E-5 4.252914830371051 9.030433334373983E-5 58-59 1.4226996196639492 9.675464286829267E-5 1.290061904910569E-5 4.678067631753377 9.030433334373983E-5 60-61 1.4303238855219707 1.0320495239284552E-4 1.290061904910569E-5 5.0727814224893395 9.030433334373983E-5 62-63 1.4362839715226574 1.0320495239284552E-4 1.290061904910569E-5 5.4847433408939565 1.1610557144195121E-4 64-65 1.4407862875707953 1.0320495239284552E-4 1.290061904910569E-5 5.937116448469896 1.1610557144195121E-4 66-67 1.446804426357203 1.0320495239284552E-4 1.290061904910569E-5 6.403002954499774 1.3545650001560975E-4 68-69 1.4532418352627068 1.0320495239284552E-4 1.290061904910569E-5 6.931915434894059 1.419068095401626E-4 70-71 1.4618917003351322 1.1610557144195121E-4 1.290061904910569E-5 7.525666426629148 1.419068095401626E-4 72-73 1.48370664714717 1.290061904910569E-4 1.290061904910569E-5 8.048683324117992 1.548074285892683E-4 74-75 1.5047153052686386 1.3545650001560975E-4 1.290061904910569E-5 8.566036849844277 1.6125773811382114E-4 76-77 1.5116429376980083 1.419068095401626E-4 1.290061904910569E-5 9.1347864418622 1.67708047638374E-4 78-79 1.51709989955578 1.419068095401626E-4 1.290061904910569E-5 9.711966588428712 1.67708047638374E-4 80-81 1.5276203543903257 1.548074285892683E-4 1.290061904910569E-5 10.339117282881936 1.8060866668747965E-4 82-83 1.5316904997003187 1.548074285892683E-4 1.290061904910569E-5 11.026700927270696 2.3221114288390243E-4 84-85 1.5461520936543662 1.548074285892683E-4 1.290061904910569E-5 11.691521429347308 2.3221114288390243E-4 86-87 1.5716824187525462 1.548074285892683E-4 1.290061904910569E-5 12.366913988734662 2.3221114288390243E-4 88 1.5779263183723133 1.548074285892683E-4 1.290061904910569E-5 12.89918708039124 2.451117619330081E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 2410 0.0 26.526424 3 GATCTAC 3680 0.0 21.983566 1 AGTACTT 30050 0.0 20.572428 12-13 CGATCTC 2690 0.0 20.264397 5 CCGATCT 3380 0.0 19.465328 4 TCTACAC 5180 0.0 19.328878 3 GAGTACT 28930 0.0 19.20031 12-13 TACACTC 4690 0.0 18.93725 5 ATCTACA 4380 0.0 18.362825 2 TCTCGCG 1110 0.0 18.204512 8 TACTTTT 34605 0.0 18.014591 14-15 CGTTAAC 780 0.0 17.487225 1 GTACTTT 32415 0.0 17.223698 14-15 GTATAAG 2340 0.0 16.683216 1 CTACACT 6410 0.0 16.055967 4 ACTTTTT 36095 0.0 15.761347 16-17 CATGGGG 18910 0.0 15.656669 4 GTATATA 1355 0.0 14.926113 1 AGTACTC 2515 0.0 14.7610445 5 CATGGGT 8135 0.0 14.731026 4 >>END_MODULE