FastQCFastQC Report
Fri 27 May 2016
ERR765289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13601354
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT317880.2337120260232915No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA291700.21446394233985822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG52400.019.7356031
GTATAAG51350.019.5899071
TAGTACT56650.018.4169544
TAGGCAT71100.018.3729655
GTACAAG67600.017.5927121
GTACATG936800.017.5722871
CATGGGG306050.017.3827634
TCTAACG24900.017.177042
TACATGG930350.017.1311972
CGTTAAC14900.016.7204741
GTACAAA66700.016.561551
GTATCAA674050.016.381361
ACATGGG864150.016.3213463
CTAGGCA80950.016.3137654
ATTTAGG64650.015.9233551
CTAGTAC64400.015.9092533
CTAACGC26650.015.6953833
GGGCTAT70750.015.6737776
TTTAGGG74150.015.4662482
TGATCGC34750.015.4124299
Produced by FastQC (version 0.11.5)