##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765289_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13601354
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432161827418064	33.0	33.0	33.0	27.0	33.0
2	31.359496782452688	33.0	33.0	33.0	27.0	33.0
3	31.28190002260069	33.0	33.0	33.0	27.0	33.0
4	34.844258299578115	37.0	37.0	37.0	33.0	37.0
5	35.09020954825527	37.0	37.0	37.0	33.0	37.0
6	35.09476725625993	37.0	37.0	37.0	33.0	37.0
7	34.94787107224766	37.0	37.0	37.0	33.0	37.0
8	34.93738888054822	37.0	37.0	37.0	33.0	37.0
9	34.65085351061372	37.0	37.0	37.0	27.0	37.0
10-11	34.64214084862434	37.0	37.0	37.0	27.0	37.0
12-13	34.63083723135212	37.0	37.0	37.0	27.0	37.0
14-15	35.833376809397066	37.0	37.0	40.0	27.0	40.0
16-17	36.06449019707891	37.0	37.0	40.0	27.0	40.0
18-19	35.86069732469282	37.0	37.0	40.0	27.0	40.0
20-21	35.83075155605832	37.0	37.0	40.0	27.0	40.0
22-23	35.994949583695856	37.0	37.0	40.0	27.0	40.0
24-25	36.008024936340895	37.0	37.0	40.0	27.0	40.0
26-27	35.683262195807856	37.0	37.0	40.0	27.0	40.0
28-29	35.560960033831925	37.0	35.0	40.0	27.0	40.0
30-31	35.3571762414242	37.0	33.0	40.0	27.0	40.0
32-33	35.30906639883059	37.0	33.0	40.0	27.0	40.0
34-35	35.26252397371614	37.0	33.0	40.0	27.0	40.0
36-37	35.03934828841305	37.0	33.0	40.0	27.0	40.0
38-39	34.92305166823832	37.0	33.0	40.0	27.0	40.0
40-41	35.06111630503845	37.0	33.0	40.0	27.0	40.0
42-43	34.98465244710196	37.0	33.0	40.0	27.0	40.0
44-45	34.928700701415465	37.0	33.0	40.0	27.0	40.0
46-47	34.89585231735017	37.0	33.0	40.0	27.0	40.0
48-49	34.72927294591406	37.0	33.0	40.0	27.0	40.0
50-51	34.57499235002633	37.0	33.0	40.0	27.0	40.0
52-53	34.45223335853181	37.0	33.0	40.0	27.0	40.0
54-55	34.328647243502374	37.0	33.0	40.0	27.0	40.0
56-57	34.12910733004964	37.0	33.0	40.0	27.0	40.0
58-59	33.928057750720996	37.0	33.0	37.0	24.5	40.0
60-61	33.654635339981596	37.0	33.0	37.0	22.0	40.0
62-63	33.42524876567436	37.0	33.0	37.0	22.0	40.0
64-65	33.21757848520082	37.0	33.0	37.0	22.0	40.0
66-67	32.985214266167915	37.0	33.0	37.0	22.0	40.0
68-69	32.77644229390692	37.0	33.0	37.0	22.0	38.5
70-71	32.53344946392837	37.0	33.0	37.0	22.0	37.0
72-73	32.28133820353474	33.0	33.0	37.0	22.0	37.0
74-75	32.048924467372885	33.0	33.0	37.0	22.0	37.0
76-77	31.839140941409216	33.0	33.0	37.0	22.0	37.0
78-79	31.6357072979646	33.0	33.0	37.0	22.0	37.0
80-81	31.479272284215234	33.0	33.0	37.0	22.0	37.0
82-83	31.32026807772226	33.0	27.0	37.0	22.0	37.0
84-85	31.147102634046583	33.0	27.0	37.0	22.0	37.0
86-87	30.940269365829316	33.0	27.0	37.0	18.5	37.0
88-89	30.78054570890516	33.0	27.0	37.0	15.0	37.0
90-91	30.662988442180094	33.0	27.0	37.0	15.0	37.0
92-93	30.55042755302156	33.0	27.0	37.0	15.0	37.0
94-95	30.396423032589254	33.0	27.0	37.0	15.0	37.0
96-97	30.270196261342804	33.0	27.0	37.0	15.0	37.0
98-99	30.121873932551125	33.0	27.0	37.0	15.0	37.0
100	30.026390534354153	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	10.0
7	278.0
8	1789.0
9	3966.0
10	6696.0
11	11894.0
12	21482.0
13	33455.0
14	44758.0
15	51537.0
16	59078.0
17	67771.0
18	79853.0
19	92391.0
20	106533.0
21	123042.0
22	140036.0
23	148808.0
24	156235.0
25	173520.0
26	200054.0
27	239834.0
28	291516.0
29	357385.0
30	440576.0
31	546081.0
32	696535.0
33	899477.0
34	1160188.0
35	1529902.0
36	2156187.0
37	2787939.0
38	971819.0
39	728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.188857404179274	18.516696989967933	12.486481770784676	25.807963835068115
2	16.170131965642913	20.437440510592396	37.460410564517204	25.932016959247488
3	18.184369281272854	25.568207765390305	29.007498300353383	27.239924652983454
4	13.049147445277375	17.201296985080347	36.155268163549195	33.59428740609308
5	14.54116394050298	36.73294498402819	33.32338672027151	15.402504355197328
6	32.17075174273618	36.15169140460969	17.185176796071737	14.492380056582391
7	27.947983968699464	31.296623562716373	22.006082537585545	18.749309930998617
8	27.159222454631227	32.83401752165208	19.90651991567396	20.100240108042737
9	27.12794471810003	14.233971664239734	19.459915512540224	39.178168105120015
10-11	25.450530266558165	24.90150785040369	26.961931857784275	22.68603002525387
12-13	26.65000785224371	22.76711377401184	26.36383841788171	24.219039955862744
14-15	24.39283570281864	23.47436651053907	24.274899998566287	27.857897788076
16-17	24.02794526619464	26.03579530460883	25.176220362822004	24.760039066374528
18-19	24.36235867083279	25.46497129200024	26.843192033852205	23.32947800331477
20-21	25.398375099222225	24.8219588109796	26.05275006638712	23.72691602341106
22-23	24.988342734275008	24.702674460973252	25.594424955409718	24.714557849342018
24-25	24.550209628111418	25.15447617240616	25.763405204778095	24.531908994704327
26-27	24.651077498248856	24.913249930253926	25.933224904402742	24.502447667094476
28-29	24.31354842468098	24.94474919894981	26.275968759152995	24.465733617216213
30-31	24.776754331074287	24.88525253709532	26.10281409452819	24.235179037302206
32-33	24.322163005975835	25.071122382130362	25.791161340845285	24.815553271048522
34-35	24.519036833496777	25.064413823175556	26.23668361713077	24.179865726196898
36-37	24.746579234329573	24.83335202855404	25.926680409186282	24.493388327930106
38-39	24.75449929832772	24.586637897012004	26.072644211731493	24.586218592928784
40-41	24.511853036522137	24.76952270626382	26.201706614225245	24.51691764298879
42-43	24.906346411554072	24.76195103725255	25.743517371565012	24.58818517962837
44-45	24.609164479971046	25.027204038379914	25.621260640140893	24.742370841508155
46-47	24.350876335982093	24.91735944852987	25.89309288825205	24.838671327235986
48-49	24.845625560966997	25.00936538999161	25.50125068051734	24.64375836852405
50-51	24.115971100224876	25.316424089696504	25.292543442625842	25.27506136745277
52-53	23.991552285854766	25.284758364312264	25.096316991466814	25.627372358366152
54-55	24.201410388995097	25.17221447217681	25.07835616954018	25.54801896928791
56-57	24.412569513299925	24.720027873695514	25.056744350599214	25.810658262405344
58-59	24.012510078040762	24.716656885777695	25.69530577617493	25.575527260006613
60-61	24.117209213141575	24.960592158692435	25.33216545940941	25.59003316875658
62-63	23.788681575808152	24.850780533442638	25.258462901455932	26.102074989293282
64-65	23.616275690736803	24.707680187834608	25.40513049185824	26.270913629570348
66-67	24.254770875453225	24.586830960723727	25.175274561000716	25.98312360282233
68-69	24.063227315191842	24.32793231851196	25.305531428765534	26.303308937530673
70-71	23.617694356274022	24.45253106448867	25.40163222858332	26.528142350653987
72-73	24.100252108069004	24.400943732625755	25.415765099097072	26.08303906020817
74-75	24.1126155942326	24.368571823280178	25.4288195972326	26.08999298525462
76-77	23.712553815027942	24.456186673727736	25.64590072791014	26.185358783334177
78-79	23.752265675333916	24.522192755382495	25.544370475496077	26.18117109378751
80-81	23.498610100896624	24.465797394919782	25.78768117598941	26.247911328194185
82-83	23.60753997184392	24.374406491603317	25.605139617290202	26.412913919262564
84-85	23.71242277643008	24.456207807641906	25.28117700423592	26.55019241169209
86-87	23.49606777645686	24.224688803895702	25.592274559146354	26.686968860501075
88-89	23.618597084683234	23.805914303897833	25.727480024986416	26.84800858643252
90-91	23.776612455968653	23.933705913782706	25.571915842433334	26.717765787815313
92-93	23.613619592401285	23.98724090087766	25.5392227378331	26.859916768887953
94-95	23.08723259091853	24.0006206917316	25.918048619308298	26.994098098041576
96-97	23.55159412369526	23.838980436077986	25.620672639473895	26.988752800752852
98-99	23.337244507656532	23.970829749438167	25.55786437015039	27.134061372754914
100	23.37539093779412	23.93421223769018	25.546842356367925	27.143554468147773
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	474.0
1	468.0
2	575.0
3	748.0
4	705.5
5	667.5
6	848.0
7	1475.0
8	2380.0
9	2518.5
10	1960.5
11	1595.5
12	1593.0
13	1741.0
14	2032.5
15	2465.5
16	2953.0
17	3533.0
18	4334.5
19	5380.5
20	6634.0
21	8185.5
22	10489.5
23	13762.5
24	17508.0
25	22020.0
26	28767.0
27	36410.0
28	44820.0
29	57629.0
30	73224.5
31	88388.0
32	106482.0
33	125372.5
34	144857.0
35	164063.5
36	181935.0
37	206704.5
38	226156.0
39	238104.0
40	248316.0
41	258913.5
42	278207.5
43	312647.0
44	358007.0
45	408239.5
46	465738.5
47	521376.5
48	576722.5
49	622046.5
50	668527.0
51	681537.5
52	648215.0
53	631596.0
54	621190.5
55	591887.0
56	556580.0
57	511194.0
58	465878.5
59	424699.5
60	368618.0
61	306583.5
62	251138.0
63	203940.5
64	161325.5
65	126355.5
66	99239.0
67	76824.5
68	60525.5
69	49327.5
70	40961.5
71	34495.0
72	28763.5
73	25972.0
74	21321.5
75	16020.5
76	11993.5
77	8501.0
78	6006.0
79	3943.5
80	2612.5
81	1832.5
82	1298.0
83	870.0
84	593.0
85	395.5
86	245.5
87	143.0
88	89.5
89	52.5
90	35.0
91	28.5
92	19.0
93	10.0
94	8.0
95	5.5
96	5.5
97	3.5
98	4.0
99	3.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0496127076760152
2	0.0245931397712316
3	0.021608142836367614
4	0.022409533639077404
5	0.015329356180274404
6	0.02561509684991656
7	0.04925244942525575
8	0.005955289451329625
9	0.0038157965743704633
10-11	0.0019850964837765414
12-13	0.001073422543079167
14-15	0.002198310550552541
16-17	0.006697862580446035
18-19	0.005164926962418595
20-21	0.00882632714360644
22-23	0.018667259156698663
24-25	0.025468052665933112
26-27	0.02804500199024303
28-29	0.04467202309417136
30-31	0.04218330028025151
32-33	0.0398710304871118
34-35	0.047392340497865136
36-37	0.04469407972176888
38-39	0.054413700283074753
40-41	0.05154266259079795
42-43	0.05715975041896565
44-45	0.05722959640635778
46-47	0.06213351994220575
48-49	0.064361239329555
50-51	0.03359959604021776
52-53	3.0511668176565367E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.808245267346178E-4
64-65	1.9483354377806798E-4
66-67	3.71286564558205E-4
68-69	0.001290312714454752
70-71	8.455040579048233E-5
72-73	3.2349720476358457E-4
74-75	3.676104599586188E-5
76-77	1.3601587018468897E-4
78-79	7.352209199172377E-6
80-81	5.146546439420664E-5
82-83	2.205662759751713E-4
84-85	0.0013197215512514417
86-87	0.0038599098295654974
88-89	0.0026614997301004
90-91	0.003918727503158877
92-93	0.004047391164144393
94-95	0.0029372075750693644
96-97	0.002378439675932264
98-99	0.004260605230920392
100	0.0029776447256648123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3601354E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.03658636522901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5947218438723	29.46481437127201
2	12.910979577620063	10.338230978381874
3	5.03265118853978	6.044705218681361
4	2.542177578126028	4.071204486495658
5	1.3881902826257813	2.778920007085939
6	0.8997615405807646	2.161402837654398
7	0.6138706552642527	1.7204099854596886
8	0.4404129314356773	1.4106104292630544
9	0.33276733932237806	1.1990581488277074
>10	1.9447197374652665	14.411326064017654
>50	0.16392419696220534	4.5635588435666685
>100	0.10924849605553583	8.71643786689498
>500	0.016524620138218763	4.60197795591208
>1k	0.009612231269817103	6.798529648187487
>5k	3.616449442380315E-4	1.0691772278241898
>10k+	7.613577773432242E-5	0.6496359304753783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	31788	0.2337120260232915	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	29170	0.21446394233985822	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4704418398344754E-5	0.0
2	0.0	0.0	0.0	2.2056627597517128E-5	0.0
3	0.0	0.0	0.0	2.2056627597517128E-5	0.0
4	0.0	7.352209199172377E-6	0.0	2.2056627597517128E-5	0.0
5	0.0	1.4704418398344754E-5	0.0	4.4113255195034256E-5	0.0
6	7.352209199172377E-6	1.4704418398344754E-5	0.0	4.4113255195034256E-5	1.4704418398344754E-5
7	7.352209199172377E-6	1.4704418398344754E-5	0.0	4.4113255195034256E-5	1.4704418398344754E-5
8	7.352209199172377E-6	1.4704418398344754E-5	0.0	4.4113255195034256E-5	1.4704418398344754E-5
9	1.4704418398344754E-5	1.4704418398344754E-5	0.0	4.4113255195034256E-5	1.4704418398344754E-5
10-11	2.9408836796689507E-5	1.4704418398344754E-5	0.0	4.4113255195034256E-5	1.4704418398344754E-5
12-13	3.676104599586188E-5	1.4704418398344754E-5	0.0	5.146546439420664E-5	1.4704418398344754E-5
14-15	4.0437150595448064E-5	1.4704418398344754E-5	0.0	9.55787195892409E-5	2.573273219710332E-5
16-17	4.4113255195034256E-5	1.4704418398344754E-5	0.0	1.3969197478427516E-4	3.676104599586188E-5
18-19	5.514156899379283E-5	1.4704418398344754E-5	0.0	1.543963931826199E-4	3.676104599586188E-5
20-21	7.352209199172376E-5	1.4704418398344754E-5	0.0	3.014405771660674E-4	3.676104599586188E-5
22-23	7.719819659130995E-5	1.4704418398344754E-5	0.0	0.001249875563859304	4.778935979462045E-5
24-25	8.455040579048232E-5	1.4704418398344754E-5	0.0	0.003554793147799844	5.8817673593379015E-5
26-27	8.822651039006851E-5	1.4704418398344754E-5	0.0	0.006319223806688657	5.8817673593379015E-5
28-29	8.822651039006851E-5	1.4704418398344754E-5	0.0	0.01924808368343328	6.24937781929652E-5
30-31	8.822651039006851E-5	1.4704418398344754E-5	0.0	0.06199382796742148	8.087430119089613E-5
32-33	8.822651039006851E-5	1.4704418398344754E-5	0.0	0.15387806243407826	9.55787195892409E-5
34-35	9.19026149896547E-5	1.4704418398344754E-5	0.0	0.2796339246813222	9.55787195892409E-5
36-37	9.55787195892409E-5	1.4704418398344754E-5	0.0	0.47067372851261724	9.55787195892409E-5
38-39	1.0660703338799947E-4	1.4704418398344754E-5	0.0	0.7672471431888326	9.55787195892409E-5
40-41	1.1028313798758565E-4	1.4704418398344754E-5	0.0	1.118362921809108	1.0293092878841328E-4
42-43	1.2131145178634421E-4	1.838052299793094E-5	0.0	1.4445583873487888	1.1028313798758566E-4
44-45	1.3969197478427516E-4	2.9408836796689507E-5	0.0	1.8265534446055884	1.2131145178634421E-4
46-47	1.3969197478427516E-4	3.676104599586188E-5	0.0	2.2523272315388603	1.286636609855166E-4
48-49	1.3969197478427516E-4	3.676104599586188E-5	0.0	2.6921033008919553	1.3969197478427516E-4
50-51	1.4704418398344752E-4	3.676104599586188E-5	0.0	3.1548770806200617	1.3969197478427516E-4
52-53	1.4704418398344752E-4	3.676104599586188E-5	0.0	3.6196139002043473	1.5439639318261993E-4
54-55	1.507202885830337E-4	3.676104599586188E-5	0.0	4.090471433946944	1.7277691618055083E-4
56-57	1.543963931826199E-4	3.676104599586188E-5	0.0	4.620966412608627	1.7645302078013702E-4
58-59	1.543963931826199E-4	3.676104599586188E-5	0.0	5.1880055470947966	1.838052299793094E-4
60-61	1.543963931826199E-4	3.676104599586188E-5	0.0	5.7519199926713185	1.9850964837765417E-4
62-63	1.543963931826199E-4	3.676104599586188E-5	0.0	6.323477059710379	2.0586185757682655E-4
64-65	1.543963931826199E-4	3.676104599586188E-5	7.352209199172377E-6	6.918289164446422	2.0586185757682655E-4
66-67	1.543963931826199E-4	3.676104599586188E-5	7.352209199172377E-6	7.560129675324971	2.0586185757682655E-4
68-69	1.543963931826199E-4	3.676104599586188E-5	7.352209199172377E-6	8.248917718044837	2.0586185757682655E-4
70-71	1.6174860238179229E-4	3.676104599586188E-5	7.352209199172377E-6	8.992292237963955	2.205662759751713E-4
72-73	1.6174860238179229E-4	3.676104599586188E-5	7.352209199172377E-6	9.707897463737801	2.499751127718608E-4
74-75	1.6174860238179229E-4	3.676104599586188E-5	7.352209199172377E-6	10.413827182205537	2.573273219710332E-4
76-77	1.6910081158096467E-4	3.676104599586188E-5	7.352209199172377E-6	11.165928774444074	2.6835563576979175E-4
78-79	1.6910081158096467E-4	3.676104599586188E-5	7.352209199172377E-6	11.935683020969824	2.7938394956855033E-4
80-81	1.7645302078013702E-4	3.676104599586188E-5	7.352209199172377E-6	12.749929161464365	2.7938394956855033E-4
82-83	1.7645302078013702E-4	3.676104599586188E-5	7.352209199172377E-6	13.594764168332064	2.7938394956855033E-4
84-85	1.838052299793094E-4	4.4113255195034256E-5	7.352209199172377E-6	14.41591403326463	2.7938394956855033E-4
86-87	1.838052299793094E-4	5.146546439420664E-5	7.352209199172377E-6	15.24883478512507	2.7938394956855033E-4
88	1.838052299793094E-4	5.146546439420664E-5	7.352209199172377E-6	15.897123183471292	2.7938394956855033E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	5240	0.0	19.735603	1
GTATAAG	5135	0.0	19.589907	1
TAGTACT	5665	0.0	18.416954	4
TAGGCAT	7110	0.0	18.372965	5
GTACAAG	6760	0.0	17.592712	1
GTACATG	93680	0.0	17.572287	1
CATGGGG	30605	0.0	17.382763	4
TCTAACG	2490	0.0	17.17704	2
TACATGG	93035	0.0	17.131197	2
CGTTAAC	1490	0.0	16.720474	1
GTACAAA	6670	0.0	16.56155	1
GTATCAA	67405	0.0	16.38136	1
ACATGGG	86415	0.0	16.321346	3
CTAGGCA	8095	0.0	16.313765	4
ATTTAGG	6465	0.0	15.923355	1
CTAGTAC	6440	0.0	15.909253	3
CTAACGC	2665	0.0	15.695383	3
GGGCTAT	7075	0.0	15.673777	6
TTTAGGG	7415	0.0	15.466248	2
TGATCGC	3475	0.0	15.412429	9
>>END_MODULE