FastQCFastQC Report
Fri 27 May 2016
ERR765288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11334196
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT486850.4295408337741821No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA465300.41052757513633964No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG298160.2630623292556437No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG222260.19609683827595709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193320.17056348769687765No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG174400.15387064067005723No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC166450.1468564686899715No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT157380.13885413663218812No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC147130.12981070734968764No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC138030.1217819067183945No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA126150.11130035160853051No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA121020.10677422553836197No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC120850.10662423695514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117330.10351859099666177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC17550.021.9570481
CTATTGA62100.021.5017099
GTACTAG48200.020.7668111
TAGTACT53850.020.5103514
TCTACAC111100.020.3458543
GATCTAC95750.020.1225191
CTACACT134900.019.8936774
TACACTC105800.019.5952685
GTATAAG46450.019.424631
AGGGCTA69500.019.4131225
ATTTAGG70450.019.144241
TTTAGGG71600.018.8356762
GTATATA23300.018.757041
GTACAAA44000.018.3701631
CATGGGG269150.018.10824
TATTCGC11400.017.7341279
TCTAACG31300.017.5652312
ATCTACA113800.017.5079772
GTGATCG38750.017.4717738
TAGGCAT70800.017.4630785
Produced by FastQC (version 0.11.5)