##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765288_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11334196
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07180156404566	33.0	33.0	33.0	33.0	33.0
2	32.030593612462674	33.0	33.0	33.0	33.0	33.0
3	32.04026028842275	33.0	33.0	33.0	33.0	33.0
4	35.81114884549376	37.0	37.0	37.0	33.0	37.0
5	35.8135654262552	37.0	37.0	37.0	33.0	37.0
6	35.756324312725845	37.0	37.0	37.0	33.0	37.0
7	35.71280838976139	37.0	37.0	37.0	33.0	37.0
8	35.69446857986222	37.0	37.0	37.0	33.0	37.0
9	35.68986825355764	37.0	37.0	37.0	33.0	37.0
10-11	35.65987958034253	37.0	37.0	37.0	33.0	37.0
12-13	35.63429611593094	37.0	37.0	37.0	33.0	37.0
14-15	37.2647166151	40.0	37.0	40.0	33.0	40.0
16-17	37.30878414313639	40.0	37.0	40.0	33.0	40.0
18-19	37.35105666074594	40.0	37.0	40.0	33.0	40.0
20-21	37.36171714341273	40.0	37.0	40.0	33.0	40.0
22-23	37.34536869664156	40.0	37.0	40.0	33.0	40.0
24-25	37.31747880484862	40.0	37.0	40.0	33.0	40.0
26-27	37.29284304771154	40.0	37.0	40.0	33.0	40.0
28-29	37.26985156247518	40.0	37.0	40.0	33.0	40.0
30-31	37.18282223106077	40.0	37.0	40.0	33.0	40.0
32-33	37.071564802655615	38.5	37.0	40.0	33.0	40.0
34-35	36.96092867989931	37.0	37.0	40.0	33.0	40.0
36-37	36.892260730271474	37.0	37.0	40.0	33.0	40.0
38-39	36.78959592722765	37.0	37.0	40.0	33.0	40.0
40-41	36.63451929011991	37.0	37.0	40.0	33.0	40.0
42-43	36.514784506991056	37.0	37.0	40.0	33.0	40.0
44-45	36.34772223808376	37.0	37.0	40.0	33.0	40.0
46-47	36.11823295626792	37.0	37.0	40.0	33.0	40.0
48-49	35.87290686520684	37.0	37.0	40.0	27.0	40.0
50-51	35.642433614170784	37.0	37.0	40.0	27.0	40.0
52-53	35.462347351325135	37.0	35.0	40.0	27.0	40.0
54-55	35.32976384032886	37.0	33.0	40.0	27.0	40.0
56-57	35.15797344602123	37.0	33.0	40.0	27.0	40.0
58-59	34.971631644626584	37.0	33.0	40.0	27.0	40.0
60-61	34.69671518826743	37.0	33.0	37.0	27.0	40.0
62-63	34.47129046471404	37.0	33.0	37.0	27.0	40.0
64-65	34.240754836073066	37.0	33.0	37.0	27.0	40.0
66-67	34.01017663714303	37.0	33.0	37.0	27.0	40.0
68-69	33.77063812025132	37.0	33.0	37.0	27.0	40.0
70-71	33.55872992667499	37.0	33.0	37.0	27.0	37.0
72-73	33.31038981503408	37.0	33.0	37.0	27.0	37.0
74-75	33.12511240320884	37.0	33.0	37.0	27.0	37.0
76-77	32.922244859714795	37.0	33.0	37.0	27.0	37.0
78-79	32.72824371486077	35.0	33.0	37.0	27.0	37.0
80-81	32.56644489825304	33.0	33.0	37.0	24.5	37.0
82-83	32.40621266828278	33.0	33.0	37.0	22.0	37.0
84-85	32.260967826919526	33.0	33.0	37.0	22.0	37.0
86-87	32.13005854142632	33.0	33.0	37.0	22.0	37.0
88-89	32.02303171746809	33.0	33.0	37.0	22.0	37.0
90-91	31.9037207844297	33.0	33.0	37.0	22.0	37.0
92-93	31.74351568474729	33.0	33.0	37.0	22.0	37.0
94-95	31.667666325869078	33.0	33.0	37.0	22.0	37.0
96-97	31.50446026343642	33.0	33.0	37.0	22.0	37.0
98-99	31.392868978090725	33.0	33.0	37.0	22.0	37.0
100	31.297595435970933	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	11.0
7	223.0
8	1468.0
9	2014.0
10	2156.0
11	4259.0
12	10372.0
13	19587.0
14	25944.0
15	26688.0
16	27838.0
17	32275.0
18	39685.0
19	47216.0
20	58149.0
21	74915.0
22	86906.0
23	84999.0
24	81324.0
25	83013.0
26	88287.0
27	103266.0
28	129321.0
29	166544.0
30	220235.0
31	295183.0
32	406725.0
33	567135.0
34	827052.0
35	1325776.0
36	2385521.0
37	3226073.0
38	883760.0
39	276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4002695474878	19.036641302751672	12.672976699462318	24.89011245029821
2	16.137053069594174	21.082144665952555	37.09978982513663	25.681012439316643
3	18.401329783925114	25.96662886839274	29.396127351976343	26.2359139957058
4	13.18413541991583	17.302313720628483	36.16571715721591	33.347833702239775
5	13.939988728264252	37.41241219821701	33.28180724731842	15.365791826200317
6	31.557381316287724	37.12047214956062	16.847636861025656	14.474509673126004
7	26.790606900547363	32.4070331331135	21.796237780091367	19.00612218624777
8	26.869080639845688	32.90317827203545	19.913333089209477	20.314407998909385
9	26.984611857919695	14.43816591706397	19.704046566337876	38.87317565867846
10-11	25.236492375064152	25.791707907084295	26.537543525831765	22.434256192019795
12-13	26.460656322653975	23.84733498271992	26.310179877160035	23.381828817466072
14-15	24.390815852957076	23.869511741596934	24.55788200742166	27.181790398024326
16-17	23.45024509762833	26.667691344189564	25.560130821085213	24.321932737096894
18-19	23.969621086869825	26.17578383051787	27.43877465346077	22.41582042915154
20-21	25.098680322869004	25.263349354966515	26.371123244670336	23.266847077494145
22-23	24.82817107267403	25.202808270269212	25.70796676649904	24.26105389055771
24-25	24.259938513054948	25.72652232422205	25.743515760132446	24.270023402590553
26-27	24.370924768250767	25.367658671443788	25.86387722341715	24.397539336888293
28-29	24.027722382842416	25.155265980905185	26.84025970135835	23.976751934894054
30-31	24.684463323666964	25.046251678053284	26.42379646905333	23.845488529226422
32-33	23.856879522616996	25.733688773485706	26.010006913689544	24.399424790207753
34-35	24.131934533292192	25.63190369505599	26.481261072978267	23.754900698673552
36-37	24.427861745692823	25.302643173866308	26.055698502924717	24.213796577516156
38-39	24.587545123367534	24.73355899153065	26.26382156458556	24.415074320516254
40-41	24.1623206964659	24.91801697573419	26.512563965201412	24.407098362598497
42-43	24.935145111544585	24.950007782057224	26.088777744092784	24.026069362305407
44-45	24.426759434881625	25.203712057959194	26.163767968760958	24.20576053839822
46-47	24.060323869884844	25.084650598384627	26.424968284427774	24.430057247302752
48-49	24.73301114698068	25.11398541235569	26.099419784832882	24.053583655830746
50-51	23.980567738415175	25.44579619137132	25.584855203039393	24.988780867174114
52-53	23.627811669647954	25.805252303475445	25.046682827690947	25.520253199185657
54-55	23.934181322380883	25.501942188534	24.975045583371625	25.588830905713493
56-57	24.424045315562097	24.80003960249612	25.149526336321728	25.626388745620055
58-59	23.820087108136917	24.770635239970918	26.06912178903454	25.340155862857628
60-61	24.14613089157431	25.184151713579432	25.43011821718756	25.239599177658693
62-63	23.630504610774857	25.143677767887812	25.371187605322298	25.854630016015033
64-65	23.10442809676652	25.260024197982982	25.55404592675522	26.081501778495277
66-67	24.27972920777912	24.953427087746448	25.213217062024242	25.553626642450194
68-69	23.992397624648135	24.615617348224912	25.467158915514922	25.92482611161203
70-71	23.61412997563394	25.037941408433255	25.335583105217246	26.012345510715555
72-73	24.4908411766481	24.821296309205167	25.3857405528595	25.302121961287234
74-75	24.39958481797494	25.031869208486036	25.43668844690018	25.131857526638846
76-77	24.01224615831777	25.09696551851322	25.533962759364258	25.35682556380475
78-79	23.882723245912917	25.27946559653274	25.483750817293082	25.35406034026126
80-81	23.699257455936774	25.23843349236326	25.740956940420755	25.32135211127921
82-83	23.948266406987585	24.841769087575035	25.715896419349953	25.49406808608743
84-85	24.17446339938973	25.144704026953686	25.072429771610793	25.608402802045795
86-87	23.785127382448746	24.842484732864165	25.556255427339515	25.816132457347575
88-89	24.007993253255222	24.24296204223756	25.769648180305403	25.979396524201814
90-91	23.964405000712897	24.53183606515721	25.629474123315344	25.87428481081455
92-93	23.97162506093859	24.638851598705205	25.627297452615906	25.762225887740303
94-95	23.18389053258745	24.709770027979	26.090323885328708	26.016015554104836
96-97	23.677188264495513	24.733750703355174	25.4890267770154	26.100034255133913
98-99	23.63757491064897	24.798871398132412	25.418701569632923	26.144852121585693
100	23.65204246631595	24.72817968402438	25.4144341074519	26.20534374220777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	829.0
1	765.0
2	757.5
3	784.0
4	620.5
5	529.0
6	649.0
7	1259.5
8	1976.5
9	1904.0
10	1377.5
11	1151.5
12	1281.0
13	1527.0
14	1897.5
15	2421.0
16	3075.0
17	3816.0
18	4792.5
19	5909.0
20	7204.5
21	8887.0
22	10810.0
23	13045.5
24	15607.0
25	19018.0
26	24438.5
27	30092.0
28	36544.5
29	48733.5
30	63464.5
31	77923.0
32	96958.5
33	116759.0
34	137786.0
35	155210.0
36	171241.5
37	198750.5
38	215124.0
39	220905.0
40	222840.0
41	223817.0
42	235097.0
43	261563.0
44	299355.0
45	341108.5
46	388156.0
47	437846.5
48	496667.5
49	544587.0
50	602359.0
51	614221.5
52	562883.0
53	535983.5
54	503644.5
55	461632.5
56	441375.5
57	396870.5
58	345087.0
59	318746.0
60	278327.0
61	226944.0
62	180102.0
63	143887.5
64	115100.0
65	87858.5
66	68697.0
67	53795.0
68	41582.5
69	34061.0
70	29198.5
71	25716.5
72	22379.0
73	20546.5
74	16595.0
75	12682.5
76	10098.5
77	7711.0
78	5820.5
79	4027.0
80	2746.0
81	2057.0
82	1566.0
83	1109.0
84	795.0
85	564.0
86	376.0
87	204.0
88	107.0
89	81.5
90	61.0
91	45.0
92	29.5
93	20.0
94	20.0
95	16.0
96	9.0
97	4.5
98	1.5
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023045304669162242
2	0.006846537681190619
3	0.010825646565490838
4	0.010155109369910315
5	0.012572572417134838
6	0.007393554867058943
7	0.010172755085583487
8	0.010155109369910315
9	0.007578834881627246
10-11	0.028947796561838175
12-13	0.031047636726945608
14-15	0.013759246796155632
16-17	0.004843748952285632
18-19	0.013521029634567817
20-21	0.0022013030302281697
22-23	2.690971640158684E-4
24-25	0.0035997259973270268
26-27	0.0015704686949122814
28-29	0.0015660572659939884
30-31	0.026340642071127057
32-33	0.020561670188163322
34-35	0.007614126312973589
36-37	0.008514057812305346
38-39	0.0060304233313064285
40-41	0.014707704013588613
42-43	0.0038114745854050873
44-45	0.00378500601189533
46-47	0.012660800995500696
48-49	0.01706781848487533
50-51	0.007746469180522377
52-53	0.021227795954825556
54-55	0.02678178496295635
56-57	0.013913646808295885
58-59	0.010199223659093242
60-61	0.008342012084491921
62-63	0.015166492621091076
64-65	0.008999314993317567
66-67	0.017336915648891196
68-69	0.005584869010558844
70-71	0.005315771846542975
72-73	0.010499200825537161
74-75	0.0032821031152099364
76-77	0.01034480081339691
78-79	0.002536571628018432
80-81	0.0070097605511674575
82-83	0.001821920143254978
84-85	0.008615520677426084
86-87	0.0032997488308831083
88-89	0.0012131429525305544
90-91	0.0011646172344293322
92-93	3.485028845451411E-4
94-95	0.0029909488066026035
96-97	0.0011337372320012818
98-99	0.004649646079880743
100	0.006970057690902821
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1334196E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.806224994458944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.20668507086707	23.68828756538905
2	14.441107014797158	9.475164117929898
3	5.244120750397493	5.161194157069531
4	2.5597913314625567	3.3590836143530507
5	1.3577001801218689	2.2270508792047736
6	0.82393996260872	1.6218215877160653
7	0.552199349830661	1.2680903278637026
8	0.3942940024834104	1.0348238207549212
9	0.2868785417773507	0.8470261788867053
>10	1.7330311030370784	10.666860150656959
>50	0.18087990067315649	4.149476373227123
>100	0.16491592234529842	11.460801285005994
>500	0.030281017363007538	6.954901170447313
>1k	0.022880717428620658	13.473674473950581
>5k	8.634232044309034E-4	1.8257124481439089
>10k+	4.317116022154517E-4	2.786031849400052
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	48685	0.4295408337741821	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	46530	0.41052757513633964	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	29816	0.2630623292556437	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	22226	0.19609683827595709	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19332	0.17056348769687765	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	17440	0.15387064067005723	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	16645	0.1468564686899715	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	15738	0.13885413663218812	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	14713	0.12981070734968764	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13803	0.1217819067183945	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	12615	0.11130035160853051	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12102	0.10677422553836197	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	12085	0.10662423695514	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11733	0.10351859099666177	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.352457642341812E-4	0.0	0.0	1.76457156731717E-5	2.6468573509757552E-5
2	0.0012263772392854331	0.0	0.0	3.52914313463434E-5	2.6468573509757552E-5
3	0.0012528458127951908	0.0	0.0	4.4114289182929256E-5	2.6468573509757552E-5
4	0.0014116572538537362	0.0	0.0	4.4114289182929256E-5	2.6468573509757552E-5
5	0.004896686099305147	0.0	0.0	1.411657253853736E-4	4.4114289182929256E-5
6	0.009572800752695648	0.0	0.0	2.5586287726098964E-4	6.176000485610096E-5
7	0.01088740657034694	0.0	0.0	2.823314507707472E-4	6.176000485610096E-5
8	0.013304869617571463	0.0	0.0	3.0880002428050477E-4	6.176000485610096E-5
9	0.014787109734117886	0.0	0.0	3.5291431346343405E-4	7.05828626926868E-5
10-11	0.028312550797603996	0.0	0.0	5.778971882963732E-4	8.381714944756558E-5
12-13	0.055769284385059154	0.0	0.0	0.0012484343838768978	1.941028724048887E-4
14-15	0.09040782425149521	0.0	0.0	0.0020424915891696246	2.3821716158781795E-4
16-17	0.10620073977898388	0.0	0.0	0.002360114471286715	2.823314507707472E-4
18-19	0.11530151763742219	0.0	0.0	0.002554217343691604	3.2203431103538355E-4
20-21	0.14888131456346793	0.0	0.0	0.00316740596333432	3.9261717372807034E-4
22-23	0.15826883530159527	0.0	0.0	0.0039349945951172895	4.2349717615612083E-4
24-25	0.20120527296334034	0.0	0.0	0.007817052043215063	4.323200339927067E-4
26-27	0.31504660762880754	0.0	0.0	0.019229418654838862	4.764343231756359E-4
28-29	0.3363405750174075	0.0	0.0	0.036235477134858085	4.8525718101222176E-4
30-31	0.34290478124782736	0.0	0.0	0.08968434990889516	4.984914677671007E-4
32-33	0.3484058331089387	0.0	0.0	0.19020758067003607	5.249600412768581E-4
34-35	0.3522790676991998	0.0	0.0	0.3311571460384133	5.734857593780803E-4
36-37	0.3595005768384454	0.0	0.0	0.5593427182660331	5.734857593780803E-4
38-39	0.36575598304458473	0.0	0.0	0.9059707455208997	6.176000485610095E-4
40-41	0.37229813213041313	0.0	0.0	1.3574716724503442	6.264229063975954E-4
42-43	0.38923360774773963	0.0	0.0	1.7068788999237352	6.396571931524741E-4
44-45	0.4095350036297237	0.0	0.0	2.0776903805086837	6.661257666622318E-4
46-47	0.41723735852106314	0.0	0.0	2.5185112380269405	6.881829112536964E-4
48-49	0.42341335900667326	0.0	0.0	2.955555912391139	7.058286269268681E-4
50-51	0.43494042277017264	0.0	0.0	3.4319505327065105	7.322972004366255E-4
52-53	0.43835928018184966	0.0	0.0	3.9303317147506536	7.631772028646761E-4
54-55	0.4488231895760405	8.82285783658585E-6	0.0	4.382516413162433	7.852343474561407E-4
56-57	0.4730331114796321	8.82285783658585E-6	0.0	4.8762126576953495	8.161143498841912E-4
58-59	0.479914940592169	8.82285783658585E-6	0.0	5.404909179266001	8.381714944756558E-4
60-61	0.4840793294910376	1.3234286754878776E-5	0.0	5.922052168499645	8.514057812305346E-4
62-63	0.4872511468832902	1.76457156731717E-5	0.0	6.451948598736072	9.352229306781001E-4
64-65	0.48980977565590006	2.6468573509757552E-5	0.0	6.994920504286321	9.572800752695647E-4
66-67	0.49290659875654175	2.6468573509757552E-5	0.0	7.558140868571534	9.705143620244435E-4
68-69	0.49569903326182113	2.6468573509757552E-5	0.0	8.182949191985035	9.793372198610294E-4
70-71	0.4994752164158799	2.6468573509757552E-5	0.0	8.901244517034996	0.0010234515090439588
72-73	0.510574371574305	2.6468573509757552E-5	0.0	9.565093986375391	0.0010763886560634737
74-75	0.5219426238967457	2.6468573509757552E-5	0.0	10.207883294059851	0.0011072686584915241
76-77	0.5252644298722202	2.6468573509757552E-5	0.0	10.91567500685536	0.001120502945246403
78-79	0.5276113100567521	2.6468573509757552E-5	0.0	11.633167451842194	0.001120502945246403
80-81	0.5328035618935829	2.6468573509757552E-5	0.0	12.424458691203153	0.001124914374164696
82-83	0.5344402020222696	3.52914313463434E-5	0.0	13.276596769634123	0.0011557943765927465
84-85	0.5408058939513662	3.52914313463434E-5	0.0	14.089076102089642	0.0011646172344293325
86-87	0.5533872892263377	3.52914313463434E-5	0.0	14.904846360518205	0.001195497236857383
88	0.5561753123026988	3.52914313463434E-5	0.0	15.54137585056761	0.0012087315236122616
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAC	1755	0.0	21.957048	1
CTATTGA	6210	0.0	21.501709	9
GTACTAG	4820	0.0	20.766811	1
TAGTACT	5385	0.0	20.510351	4
TCTACAC	11110	0.0	20.345854	3
GATCTAC	9575	0.0	20.122519	1
CTACACT	13490	0.0	19.893677	4
TACACTC	10580	0.0	19.595268	5
GTATAAG	4645	0.0	19.42463	1
AGGGCTA	6950	0.0	19.413122	5
ATTTAGG	7045	0.0	19.14424	1
TTTAGGG	7160	0.0	18.835676	2
GTATATA	2330	0.0	18.75704	1
GTACAAA	4400	0.0	18.370163	1
CATGGGG	26915	0.0	18.1082	4
TATTCGC	1140	0.0	17.734127	9
TCTAACG	3130	0.0	17.565231	2
ATCTACA	11380	0.0	17.507977	2
GTGATCG	3875	0.0	17.471773	8
TAGGCAT	7080	0.0	17.463078	5
>>END_MODULE