FastQCFastQC Report
Fri 27 May 2016
ERR765287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15103042
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA384490.25457785259419924No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT377180.24973776806023582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184680.12228000160497468No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG155410.102899799921102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG68650.020.739511
CTAACGC30000.020.6749653
CATGGGG356950.020.3376924
AATACGG29500.019.7505325
GTGATCG48000.019.4781578
GTACTAG60150.019.3736951
TCTAACG32500.019.0846462
CGTTAAC19500.019.0365771
GTATCAA768850.019.0315151
TATTCGC10900.018.9654089
CTATTGA79550.018.4858829
TGATCGC50450.018.4391149
GTACATG1157000.018.0442711
TACATGG1138750.017.8092882
TAGTACT78250.017.6540184
ACATGGG1092100.017.3824523
ATGGGGG198300.017.3210355
GGGCTAT89600.017.3038186
CTAGTAC89050.017.1491073
TTTAGGG90800.017.0773942
Produced by FastQC (version 0.11.5)