FastQCFastQC Report
Fri 27 May 2016
ERR765286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17543982
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348580.19868921434141917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199140.11350900838817551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA570150.033.650431
GTTATAT66600.030.8324181
ATCAACG682250.026.2885483
CAACGCA691250.026.0543675
AACGCAG700400.025.6176646
TCAACGC720800.024.9341624
TATCAAC798000.024.336492
GTACAAG78950.023.8071961
TTATATA97050.023.3855192
ACGCAGA774450.022.9687
CGCAGAG770850.022.904618
AATACGG69650.022.4646665
TATATAC159300.021.8864883
GCAGAGT786650.021.5606429
GTACATG1023450.021.280581
GTACATA58400.021.2418081
GGTATCA366850.021.1987291
TATACTT128900.020.9600245
TAATACG76700.020.9511974
TACATGG1016050.020.260662
Produced by FastQC (version 0.11.5)