##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765286_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17543982
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.717720298618637	33.0	33.0	33.0	27.0	33.0
2	31.588415845387892	33.0	33.0	33.0	27.0	33.0
3	31.63182657164149	33.0	33.0	33.0	27.0	33.0
4	35.34132194162078	37.0	37.0	37.0	33.0	37.0
5	35.425096879374365	37.0	37.0	37.0	33.0	37.0
6	35.41078177120793	37.0	37.0	37.0	33.0	37.0
7	35.33662466137961	37.0	37.0	37.0	33.0	37.0
8	35.31642987321806	37.0	37.0	37.0	33.0	37.0
9	35.2708130343499	37.0	37.0	37.0	33.0	37.0
10-11	35.26562065556155	37.0	37.0	37.0	33.0	37.0
12-13	35.218899990891465	37.0	37.0	37.0	33.0	37.0
14-15	36.70398382191682	37.0	37.0	40.0	33.0	40.0
16-17	36.77697446338009	37.0	37.0	40.0	33.0	40.0
18-19	36.78679925686198	38.5	37.0	40.0	33.0	40.0
20-21	36.8953473333477	40.0	37.0	40.0	33.0	40.0
22-23	36.88532557773942	40.0	37.0	40.0	33.0	40.0
24-25	36.87833346500241	37.0	37.0	40.0	33.0	40.0
26-27	36.84075881404803	37.0	37.0	40.0	33.0	40.0
28-29	36.78720093306069	37.0	37.0	40.0	33.0	40.0
30-31	36.692279751541015	37.0	37.0	40.0	33.0	40.0
32-33	36.572406224538994	37.0	37.0	40.0	33.0	40.0
34-35	36.43767674864235	37.0	37.0	40.0	33.0	40.0
36-37	36.334141559196766	37.0	37.0	40.0	33.0	40.0
38-39	36.218886538985274	37.0	37.0	40.0	33.0	40.0
40-41	36.113812816269416	37.0	37.0	40.0	27.0	40.0
42-43	35.9542057213693	37.0	37.0	40.0	27.0	40.0
44-45	35.79311065184631	37.0	37.0	40.0	27.0	40.0
46-47	35.58328354418056	37.0	37.0	40.0	27.0	40.0
48-49	35.307479026141266	37.0	33.0	40.0	27.0	40.0
50-51	35.19418670174194	37.0	33.0	40.0	27.0	40.0
52-53	35.028997835269095	37.0	33.0	40.0	27.0	40.0
54-55	34.87308819058296	37.0	33.0	40.0	27.0	40.0
56-57	34.72532393159091	37.0	33.0	40.0	27.0	40.0
58-59	34.56468796536613	37.0	33.0	40.0	27.0	40.0
60-61	34.37184368976211	37.0	33.0	40.0	27.0	40.0
62-63	34.14212793879975	37.0	33.0	37.0	27.0	40.0
64-65	33.93500389478284	37.0	33.0	37.0	27.0	40.0
66-67	33.733525832390846	37.0	33.0	37.0	27.0	40.0
68-69	33.524404721801474	37.0	33.0	37.0	24.5	40.0
70-71	33.29882848147017	37.0	33.0	37.0	22.0	40.0
72-73	33.05684692904951	37.0	33.0	37.0	22.0	37.0
74-75	32.81063879910502	37.0	33.0	37.0	22.0	37.0
76-77	32.591640626398274	37.0	33.0	37.0	22.0	37.0
78-79	32.39581398909324	37.0	33.0	37.0	22.0	37.0
80-81	32.172149429929874	35.0	33.0	37.0	22.0	37.0
82-83	31.981204210081838	33.0	33.0	37.0	22.0	37.0
84-85	31.794881942993328	33.0	33.0	37.0	22.0	37.0
86-87	31.636373059434284	33.0	33.0	37.0	22.0	37.0
88-89	31.467405660812922	33.0	33.0	37.0	18.5	37.0
90-91	31.29849677228351	33.0	33.0	37.0	15.0	37.0
92-93	31.150540310631875	33.0	33.0	37.0	15.0	37.0
94-95	30.985385102424296	33.0	33.0	37.0	15.0	37.0
96-97	30.86433812460592	33.0	27.0	37.0	15.0	37.0
98-99	30.71886599062858	33.0	27.0	37.0	15.0	37.0
100	30.62508762263892	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	20.0
7	214.0
8	1182.0
9	2422.0
10	4140.0
11	8531.0
12	19364.0
13	41931.0
14	61752.0
15	66160.0
16	70772.0
17	80506.0
18	93926.0
19	109111.0
20	127021.0
21	149265.0
22	171920.0
23	163342.0
24	147990.0
25	154639.0
26	177736.0
27	212909.0
28	265619.0
29	337711.0
30	434617.0
31	562140.0
32	729434.0
33	945644.0
34	1222581.0
35	1655118.0
36	2667336.0
37	4592178.0
38	2264213.0
39	2533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2577128435721	19.211686651974585	13.343185316615063	24.187415187838255
2	15.994117311748276	21.32133614547113	37.898118907826486	24.786427634954116
3	18.28011669191708	26.53427195181471	29.33542633033243	25.85018502593578
4	12.816693705431426	17.57275171972757	36.52096058610534	33.08959398873566
5	13.67778451061174	37.89898741750805	33.3312347876825	15.091993284197711
6	30.952425737782907	37.84468657115585	17.245771227991455	13.957116463069784
7	26.650916536508074	32.4783335961015	22.26822850137443	18.602521366015992
8	26.56166085897717	33.06305261827104	20.068032445541725	20.307254077210064
9	26.51786236442787	15.237099536467833	19.781495443850776	38.46354265525353
10-11	24.88272901784783	26.164855276299303	27.20788245222778	21.74453325362509
12-13	26.098655937973486	24.257950675051994	26.86789407330673	22.775499313667787
14-15	23.645293297724542	24.497309105766295	25.244514044759054	26.61288355175011
16-17	22.845920078883207	27.278295378998006	25.737381883167842	24.13840265895094
18-19	23.457250033708718	26.298863000839535	27.698845746763347	22.545041218688404
20-21	25.270901641271514	25.37989984249482	26.68452665867432	22.664671857559345
22-23	24.398246019703336	25.04788714153546	26.733903281070436	23.81996355769077
24-25	23.46531889621472	25.827154060124663	27.01702553394011	23.69050150972051
26-27	23.7622380193494	25.88354363419208	26.82216432296272	23.5320540234958
28-29	23.70164154391302	25.52896607861697	27.160233505705587	23.60915887176442
30-31	23.755534843319747	25.46394652811076	27.123126032244553	23.65739259632494
32-33	23.595859863297868	25.758359692988847	26.596565099403747	24.049215344309538
34-35	24.075845583757477	25.640030201460984	26.856839300071535	23.427284914710004
36-37	23.938358147931687	25.56998079303797	26.616760793626288	23.87490026540405
38-39	23.95316443882485	25.300334049420936	26.768095084787706	23.978406426966504
40-41	23.653327678013504	25.505280523753015	26.97180458346035	23.869587214773134
42-43	23.983224580589575	25.310499955717486	26.859099537942534	23.847175925750406
44-45	23.619228192511788	25.718676503196818	26.250346938332815	24.411748365958577
46-47	23.266796891134316	25.69026668974057	26.649627665334613	24.393308753790496
48-49	23.638861963932158	26.023816135321653	26.054526073033557	24.28279582771263
50-51	23.477855609672506	26.11294857418145	25.699287798306834	24.7099080178392
52-53	23.431770493676375	26.028502420471877	25.762548703921777	24.77717838192997
54-55	23.28832459384192	25.90861552607236	25.976168972958696	24.826890907127027
56-57	23.234797499038145	25.416996573454	25.83530289276827	25.512903034739594
58-59	23.128868265771	25.372248985002237	25.936345823341178	25.56253692588559
60-61	23.405574668683972	25.288464701402397	25.72952008510861	25.576440544805017
62-63	22.894175713028282	25.39618636929269	25.781815617333187	25.927822300345838
64-65	22.977326768976507	25.10644733487751	25.758969554682682	26.157256341463302
66-67	23.084793778486688	25.28435271968446	25.62783267061256	26.003020831216293
68-69	23.220092855711442	25.100461912886484	25.673461566557986	26.005983664844084
70-71	22.8909657573043	24.783958234637844	25.80865080278287	26.516425205274984
72-73	23.136771583240044	24.96574172580412	25.849207157917842	26.048279533038
74-75	23.08020791155018	25.214908655716386	25.856104077872804	25.84877935486063
76-77	23.115577259252436	25.042976016115254	25.818310004595375	26.02313672003693
78-79	23.173596277094656	25.130677022650477	25.677226578368405	26.01850012188646
80-81	23.003383036957185	25.03880555804064	25.999853564469667	25.957957840532504
82-83	23.161439189295294	24.826549146168453	25.733665455489163	26.278346209047093
84-85	23.03400779365222	24.960600810282642	25.50960161445476	26.495789781610384
86-87	22.999439053642853	24.85925467722727	25.796558362390044	26.344747906739833
88-89	23.119384793471095	24.53347961130583	25.75673172130022	26.590403873922856
90-91	23.256859506657193	24.498368957934566	25.685061465002075	26.559710070406172
92-93	23.289341496148065	24.576882924636845	25.678837263452557	26.454938315762533
94-95	22.861283443974138	24.572729801001593	25.925329099416096	26.640657655608173
96-97	23.154166377152638	24.408007698688657	25.632419764808777	26.805406159349925
98-99	23.295012758166553	24.504771380438466	25.534579006154072	26.66563685524091
100	23.093853205377073	24.597752148803675	25.547186754553614	26.76120789126564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1407.0
1	1373.0
2	1881.0
3	2468.5
4	2041.0
5	1667.5
6	2147.5
7	4050.5
8	6486.5
9	6123.0
10	3761.0
11	2668.0
12	2804.0
13	3146.0
14	3715.5
15	4466.0
16	5202.5
17	6186.5
18	7483.0
19	9142.5
20	11262.0
21	14133.0
22	18096.5
23	23120.5
24	29692.5
25	38825.5
26	50549.5
27	63104.5
28	76943.0
29	95979.5
30	117173.5
31	138220.5
32	162315.5
33	186799.0
34	212772.0
35	236524.5
36	264245.0
37	299747.5
38	322726.5
39	339140.5
40	374583.0
41	412804.0
42	435031.0
43	475362.5
44	522739.0
45	565913.5
46	641760.5
47	723542.5
48	786685.5
49	818650.5
50	858208.0
51	889742.0
52	871879.0
53	816419.0
54	744136.0
55	686295.5
56	629579.0
57	575930.5
58	533801.0
59	473133.0
60	388545.0
61	310328.5
62	248851.0
63	195694.5
64	146666.0
65	115884.0
66	97545.0
67	78591.5
68	61532.5
69	50427.5
70	42384.5
71	35470.0
72	30416.5
73	29235.5
74	23184.0
75	17117.5
76	13499.0
77	10428.5
78	8173.5
79	5849.5
80	4452.0
81	3484.5
82	2916.5
83	2653.5
84	2028.5
85	1296.0
86	906.5
87	504.0
88	224.0
89	152.5
90	121.5
91	99.5
92	71.0
93	42.0
94	34.0
95	25.0
96	16.5
97	13.0
98	10.0
99	7.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017173980228661886
2	0.005597360964004637
3	0.005905158817422407
4	0.0037106741217586745
5	0.04575928087477518
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.018471975176445E-4
18-19	0.0017185380149158842
20-21	6.526454484506425E-4
22-23	0.0051955137664869925
24-25	0.0075866470907231896
26-27	0.006039107883261622
28-29	0.022454993398876036
30-31	0.020665205880854187
32-33	0.013332207021188236
34-35	0.013944952747899536
36-37	0.013828103562805753
38-39	0.021448950415019808
40-41	0.030400737985253293
42-43	0.01823132285475441
44-45	0.017330729135495007
46-47	0.018487821065935887
48-49	0.013845203443551186
50-51	0.010433777234837564
52-53	0.02758780760262978
54-55	0.025857869667216943
56-57	0.013742604159078594
58-59	0.016720833388907945
60-61	0.017675576730527883
62-63	0.02102715335663249
64-65	0.011251721530494047
66-67	0.015865839351636362
68-69	0.00791439480501063
70-71	0.009111386457190847
72-73	0.010656075684528175
74-75	0.003742023903125299
76-77	0.0076863963950715404
78-79	0.005794009592577101
80-81	0.00242533308572706
82-83	2.0234858882094156E-4
84-85	0.0027017811577782053
86-87	0.002365483503118049
88-89	0.0012254914534226037
90-91	7.780445739171415E-4
92-93	0.0011228921689500138
94-95	0.0032774771428744054
96-97	0.0012254914534226037
98-99	0.001806887398767281
100	0.002183084775166778
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.7543982E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.84680000993947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26236388913522	25.90374506579302
2	13.64183551862374	9.780322992091882
3	5.197479701301387	5.589390462248122
4	2.4739450632193396	3.547320556672029
5	1.4712102702212908	2.6369090164595805
6	0.9370605958700994	2.01543742664101
7	0.6595288341809424	1.6549398753770945
8	0.47504270614230315	1.3623008706610868
9	0.371860618274584	1.1997011893375311
>10	2.162320272989758	14.271853137741749
>50	0.176955964956585	4.409455587558459
>100	0.13432863509005422	9.770618843635233
>500	0.020216862909370525	5.040887573489306
>1k	0.014893373050942993	10.029591308007381
>5k	7.661552275446663E-4	1.709733098509424
>10k+	1.9153880688616657E-4	1.0777929957770584
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34858	0.19868921434141917	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19914	0.11350900838817551	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.699960248477227E-6
2	0.0	0.0	0.0	0.0	5.699960248477227E-6
3	0.0	0.0	0.0	1.1399920496954454E-5	5.699960248477227E-6
4	0.0	0.0	0.0	1.1399920496954454E-5	5.699960248477227E-6
5	0.0	5.699960248477227E-6	0.0	1.1399920496954454E-5	5.699960248477227E-6
6	5.699960248477227E-6	5.699960248477227E-6	0.0	1.1399920496954454E-5	5.699960248477227E-6
7	5.699960248477227E-6	5.699960248477227E-6	0.0	1.1399920496954454E-5	1.1399920496954454E-5
8	5.699960248477227E-6	5.699960248477227E-6	0.0	1.1399920496954454E-5	1.1399920496954454E-5
9	1.1399920496954454E-5	5.699960248477227E-6	0.0	1.1399920496954454E-5	1.709988074543168E-5
10-11	1.1399920496954454E-5	5.699960248477227E-6	0.0	2.279984099390891E-5	1.709988074543168E-5
12-13	2.279984099390891E-5	1.1399920496954454E-5	0.0	2.8499801242386136E-5	1.9949860869670297E-5
14-15	5.699960248477227E-5	1.1399920496954454E-5	0.0	6.26995627332495E-5	3.134978136662475E-5
16-17	7.979944347868119E-5	1.1399920496954454E-5	0.0	9.404934409987425E-5	3.989972173934059E-5
18-19	9.404934409987424E-5	1.709988074543168E-5	0.0	1.225491453422604E-4	4.559968198781782E-5
20-21	1.1684918509378316E-4	2.8499801242386136E-5	0.0	2.1659848944213464E-4	4.559968198781782E-5
22-23	1.3394906583921485E-4	2.8499801242386136E-5	0.0	0.0012112415528014108	5.699960248477227E-5
24-25	1.9379864844822572E-4	2.8499801242386136E-5	0.0	0.004272120206233682	7.124950310596534E-5
26-27	3.4484759503287227E-4	2.8499801242386136E-5	0.0	0.0077490959578047905	7.409948323020395E-5
28-29	3.7049741615101975E-4	2.8499801242386136E-5	5.699960248477227E-6	0.030027390588978034	7.409948323020395E-5
30-31	3.76197376399497E-4	2.8499801242386136E-5	5.699960248477227E-6	0.11591724159315712	7.409948323020395E-5
32-33	3.76197376399497E-4	2.8499801242386136E-5	5.699960248477227E-6	0.2690552236088706	8.549940372715841E-5
34-35	3.76197376399497E-4	3.134978136662475E-5	5.699960248477227E-6	0.4647092091179756	9.119936397563563E-5
36-37	3.818973366479742E-4	3.419976149086336E-5	5.699960248477227E-6	0.7811368023519404	9.119936397563563E-5
38-39	3.818973366479742E-4	3.7049741615101975E-5	5.699960248477227E-6	1.2897100555620724	9.119936397563563E-5
40-41	3.875972968964515E-4	3.989972173934059E-5	5.699960248477227E-6	1.8826826201714069	9.974930434835148E-5
42-43	4.160970981388376E-4	3.989972173934059E-5	5.699960248477227E-6	2.4054516243803716	1.0544926459682871E-4
44-45	4.445968993812237E-4	3.989972173934059E-5	5.699960248477227E-6	2.9830228963983205	1.0829924472106732E-4
46-47	4.445968993812237E-4	3.989972173934059E-5	5.699960248477227E-6	3.599903944269893	1.0829924472106732E-4
48-49	4.445968993812237E-4	3.989972173934059E-5	5.699960248477227E-6	4.25360103538638	1.1399920496954454E-4
50-51	4.5029685962970096E-4	3.989972173934059E-5	5.699960248477227E-6	4.925270101166315	1.1399920496954454E-4
52-53	4.5029685962970096E-4	3.989972173934059E-5	5.699960248477227E-6	5.5427410949236045	1.25399125466499E-4
54-55	4.5029685962970096E-4	3.989972173934059E-5	5.699960248477227E-6	6.136927751065864	1.424990062119307E-4
56-57	4.5599681987817817E-4	3.989972173934059E-5	5.699960248477227E-6	6.807197476604799	1.481989664604079E-4
58-59	4.5599681987817817E-4	4.559968198781782E-5	5.699960248477227E-6	7.528687044936548	1.5389892670888514E-4
60-61	4.6739674037513264E-4	4.559968198781782E-5	5.699960248477227E-6	8.271232266426175	1.5389892670888514E-4
62-63	4.6739674037513264E-4	4.559968198781782E-5	5.699960248477227E-6	9.020112993731981	1.5389892670888514E-4
64-65	4.6739674037513264E-4	5.129964223629505E-5	5.699960248477227E-6	9.723111891017673	1.5389892670888514E-4
66-67	4.6739674037513264E-4	5.129964223629505E-5	5.699960248477227E-6	10.45231920552586	1.5674890683312374E-4
68-69	4.702467204993712E-4	5.129964223629505E-5	5.699960248477227E-6	11.262802253217085	1.5959888695736237E-4
70-71	4.7879666087208706E-4	5.699960248477228E-5	5.699960248477227E-6	12.120090524488681	1.7099880745431682E-4
72-73	4.7879666087208706E-4	6.26995627332495E-5	5.699960248477227E-6	12.953652711225992	1.7669876770279403E-4
74-75	4.816466409963257E-4	6.26995627332495E-5	5.699960248477227E-6	13.752308341401626	1.7669876770279403E-4
76-77	4.8449662112056433E-4	7.124950310596534E-5	5.699960248477227E-6	14.569893539562456	1.7669876770279403E-4
78-79	4.873466012448029E-4	8.549940372715841E-5	5.699960248477227E-6	15.430573287181895	1.7669876770279403E-4
80-81	4.958965416175187E-4	8.549940372715841E-5	5.699960248477227E-6	16.340611840573025	1.8239872795127127E-4
82-83	5.01596501865996E-4	9.404934409987424E-5	5.699960248477227E-6	17.26737122735306	1.880986881997485E-4
84-85	5.01596501865996E-4	9.974930434835148E-5	5.699960248477227E-6	18.148599331668258	1.880986881997485E-4
86-87	5.01596501865996E-4	1.0544926459682871E-4	5.699960248477227E-6	19.023332331280322	1.880986881997485E-4
88	5.072964621144732E-4	1.0829924472106732E-4	5.699960248477227E-6	19.678918959219178	1.880986881997485E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	57015	0.0	33.65043	1
GTTATAT	6660	0.0	30.832418	1
ATCAACG	68225	0.0	26.288548	3
CAACGCA	69125	0.0	26.054367	5
AACGCAG	70040	0.0	25.617664	6
TCAACGC	72080	0.0	24.934162	4
TATCAAC	79800	0.0	24.33649	2
GTACAAG	7895	0.0	23.807196	1
TTATATA	9705	0.0	23.385519	2
ACGCAGA	77445	0.0	22.968	7
CGCAGAG	77085	0.0	22.90461	8
AATACGG	6965	0.0	22.464666	5
TATATAC	15930	0.0	21.886488	3
GCAGAGT	78665	0.0	21.560642	9
GTACATG	102345	0.0	21.28058	1
GTACATA	5840	0.0	21.241808	1
GGTATCA	36685	0.0	21.198729	1
TATACTT	12890	0.0	20.960024	5
TAATACG	7670	0.0	20.951197	4
TACATGG	101605	0.0	20.26066	2
>>END_MODULE