FastQCFastQC Report
Fri 27 May 2016
ERR765285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16151764
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA337710.20908552155665472No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT307080.19012164863231037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196590.12171425981707014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAT62200.023.1182611
GTACTAG59850.022.4556711
CATGGGG369750.021.4393794
TATATAC109600.020.8803223
ATATACT78900.019.9513874
GTATCAA738950.019.631151
GTACATG1163000.019.6170481
GTATAAG69000.019.4097481
TACATGG1144400.019.3484522
TAGTACT73650.019.331934
ACATGGG1080500.018.879213
TTATATA81700.018.5204942
GTGATCG44600.018.1192878
CTAACGC28950.017.6928043
ATGGGGG190600.017.405315
CAACGCA798350.017.1218385
CTAGTAC84150.017.0319563
TCTAACG29500.016.885062
AACGCAG824900.016.802286
ATCAACG816600.016.7168923
Produced by FastQC (version 0.11.5)