##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765285_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16151764
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04979505644089	33.0	33.0	33.0	27.0	33.0
2	31.93990582081313	33.0	33.0	33.0	27.0	33.0
3	31.98203719420368	33.0	33.0	33.0	27.0	33.0
4	35.76534451593027	37.0	37.0	37.0	33.0	37.0
5	35.79098722591539	37.0	37.0	37.0	33.0	37.0
6	35.79327911180475	37.0	37.0	37.0	33.0	37.0
7	35.72413867612231	37.0	37.0	37.0	33.0	37.0
8	35.71194366138584	37.0	37.0	37.0	33.0	37.0
9	35.697022195222765	37.0	37.0	37.0	33.0	37.0
10-11	35.67010061563555	37.0	37.0	37.0	33.0	37.0
12-13	35.6285489931626	37.0	37.0	37.0	33.0	37.0
14-15	37.26906215940252	40.0	37.0	40.0	33.0	40.0
16-17	37.30577465099168	40.0	37.0	40.0	33.0	40.0
18-19	37.272795250103954	40.0	37.0	40.0	33.0	40.0
20-21	37.36195730695421	40.0	37.0	40.0	33.0	40.0
22-23	37.344294561262785	40.0	37.0	40.0	33.0	40.0
24-25	37.32765579041398	40.0	37.0	40.0	33.0	40.0
26-27	37.28447719394612	40.0	37.0	40.0	33.0	40.0
28-29	37.23998121815053	40.0	37.0	40.0	33.0	40.0
30-31	37.173280361203894	40.0	37.0	40.0	33.0	40.0
32-33	37.07857290386363	40.0	37.0	40.0	33.0	40.0
34-35	36.98039232742628	40.0	37.0	40.0	33.0	40.0
36-37	36.909931571560854	38.5	37.0	40.0	33.0	40.0
38-39	36.816632412410186	37.0	37.0	40.0	33.0	40.0
40-41	36.730949511149376	37.0	37.0	40.0	33.0	40.0
42-43	36.60367585856257	37.0	37.0	40.0	33.0	40.0
44-45	36.46498020278157	37.0	37.0	40.0	33.0	40.0
46-47	36.305328383946176	37.0	37.0	40.0	33.0	40.0
48-49	36.081483143265345	37.0	37.0	40.0	33.0	40.0
50-51	35.99079747574321	37.0	37.0	40.0	27.0	40.0
52-53	35.84186253588153	37.0	37.0	40.0	27.0	40.0
54-55	35.68805292722207	37.0	37.0	40.0	27.0	40.0
56-57	35.52737307825944	37.0	33.0	40.0	27.0	40.0
58-59	35.36341640454876	37.0	33.0	40.0	27.0	40.0
60-61	35.17718104969835	37.0	33.0	40.0	27.0	40.0
62-63	34.95162574812262	37.0	33.0	38.5	27.0	40.0
64-65	34.7559512075585	37.0	33.0	37.0	27.0	40.0
66-67	34.563918281619266	37.0	33.0	37.0	27.0	40.0
68-69	34.3622638988534	37.0	33.0	37.0	27.0	40.0
70-71	34.157309597886645	37.0	33.0	37.0	27.0	40.0
72-73	33.944484670528865	37.0	33.0	37.0	27.0	38.5
74-75	33.72892180693081	37.0	33.0	37.0	27.0	37.0
76-77	33.53709365738628	37.0	33.0	37.0	27.0	37.0
78-79	33.38015240935913	37.0	33.0	37.0	27.0	37.0
80-81	33.220344446588	37.0	33.0	37.0	27.0	37.0
82-83	33.09906252344945	37.0	33.0	37.0	27.0	37.0
84-85	32.96285501695047	37.0	33.0	37.0	27.0	37.0
86-87	32.85186859466248	37.0	33.0	37.0	27.0	37.0
88-89	32.7466943239141	33.0	33.0	37.0	27.0	37.0
90-91	32.63675726069302	33.0	33.0	37.0	27.0	37.0
92-93	32.549184163413976	33.0	33.0	37.0	27.0	37.0
94-95	32.450454266171796	33.0	33.0	37.0	22.0	37.0
96-97	32.379213038278664	33.0	33.0	37.0	22.0	37.0
98-99	32.28861073626385	33.0	33.0	37.0	22.0	37.0
100	32.236864344971856	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	9.0
7	247.0
8	1281.0
9	2460.0
10	3400.0
11	5042.0
12	8070.0
13	13203.0
14	20699.0
15	26605.0
16	31376.0
17	38580.0
18	47572.0
19	57934.0
20	68773.0
21	82974.0
22	98660.0
23	106410.0
24	109597.0
25	120032.0
26	139236.0
27	167259.0
28	206242.0
29	264239.0
30	341741.0
31	447921.0
32	596233.0
33	796742.0
34	1066377.0
35	1495601.0
36	2535584.0
37	4691599.0
38	2556394.0
39	3670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.146733884733344	19.339403658456312	12.651514818439203	24.862347638371144
2	15.911427413766372	21.26777685480871	38.03302082822522	24.787774903199697
3	18.025453713293913	26.63557952310261	29.462380890818046	25.876585872785423
4	12.719289610076817	17.470127477148658	37.53140492337069	32.27917798940384
5	13.699832830818199	37.892802359545804	33.95007372341789	14.457291086218119
6	30.760745389791484	37.81857511043376	17.89413218271391	13.52654731706085
7	26.884927243860172	31.983435369659936	23.011195557339743	18.120441829140148
8	26.500480071402727	33.09533868870298	20.67975361700431	19.724427622889984
9	26.804818346776237	14.717674180974907	20.176669248015262	38.300838224233594
10-11	24.982110313152173	25.710176300248072	27.79992946900413	21.507783917595628
12-13	25.979818674913773	23.65677829368978	27.311465794076735	23.051937237319713
14-15	23.78765254370978	24.375740012050695	24.992975999401676	26.84363144483785
16-17	23.33435056352881	27.00052124534484	25.768979283484843	23.896148907641503
18-19	23.82261444458812	26.378755453326285	27.29005187166724	22.50857823041835
20-21	25.176043001636145	25.577893225803443	26.21681808707703	23.029245685483378
22-23	24.323972157200295	25.414430422163086	26.140795823802943	24.120801596833672
24-25	23.813947357121148	25.994927921936178	26.27549989769717	23.91562482324551
26-27	24.15821408014098	25.87371772421168	26.05220086118818	23.915867334459158
28-29	23.87507838742108	25.629950312910292	26.589858527831517	23.90511277183711
30-31	24.217095384849365	25.652093598613913	26.362209660880303	23.768601355656415
32-33	23.89768500995765	25.83467895177214	25.94048937630953	24.32714666196068
34-35	24.281632531493567	25.684078627733037	26.438993511220023	23.595295329553377
36-37	24.287920713187987	25.716559281977215	26.00676513326577	23.988754871569036
38-39	24.41296069292464	25.294269729474163	26.15352390164577	24.13924567595543
40-41	24.057033862717994	25.577233324798115	26.373234842990996	23.992497969492895
42-43	24.707577574150687	25.417494756826574	26.106540491367948	23.768387177654787
44-45	24.26686552274881	25.791687520599144	25.738408787027957	24.203038169624094
46-47	23.868166037962048	25.563755057415054	26.313011364983918	24.25506753963898
48-49	24.475058876169665	25.85053487172695	25.830757259030936	23.843648993072446
50-51	24.002763317474454	26.01905381329824	25.545225484860733	24.432957384366574
52-53	23.963787770809468	25.925789794985942	25.53000244777651	24.58041998642808
54-55	24.180905227792	25.81009961739163	25.625976074931838	24.38301907988453
56-57	24.234898493340907	25.396053792149907	25.414391701394813	24.954656013114377
58-59	23.932940030351485	25.359354553365186	26.022424431288936	24.68528098499439
60-61	24.17742755221196	25.556689959707924	25.7134988435012	24.552383644578914
62-63	23.645851933591356	25.68790133789956	25.589436532433734	25.07681019607535
64-65	23.628690951128704	25.462205192950822	25.75309737417469	25.156006481745784
66-67	24.115497972493557	25.52452446691013	25.530856022258096	24.829121538338217
68-69	24.166019965324402	25.297566463906822	25.575111165708492	24.961302405060284
70-71	23.668685015642716	25.15776923182184	25.8145081750347	25.359037577500743
72-73	24.22606992016808	25.23831636281802	25.774944004280837	24.76066971273307
74-75	24.241382476547084	25.371952492166365	25.706974266724263	24.679690764562288
76-77	23.950378512628397	25.44586932583986	25.8064166596081	24.797335501923644
78-79	23.965923448774884	25.56171968800569	25.704294037631342	24.768062825588082
80-81	23.674576361853912	25.357232146162918	26.057273541950103	24.910917950033067
82-83	23.88514095829251	25.282573480682686	25.77361072449957	25.05867483652523
84-85	23.92212476389354	25.434757069030926	25.397131725387002	25.245986441688533
86-87	23.56543965260671	25.26010940119842	25.746030818301126	25.42842012789374
88-89	23.7787164198926	24.860368945272082	25.828441268835423	25.5324733659999
90-91	23.960904665691658	24.898861701154175	25.748385025278537	25.391848607875634
92-93	23.76095593652495	25.10685568146932	25.698485308661397	25.43370307334434
94-95	23.11022011654276	25.219702178275544	26.119979831888447	25.550097873293254
96-97	23.5976376011985	25.07355796029223	25.704441722586484	25.62436271592279
98-99	23.496986017651704	25.142603879776527	25.685325327788245	25.675084774783524
100	23.384283830241895	25.250230180871302	25.570821009088423	25.79466497979838
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1229.0
1	1105.0
2	1372.0
3	1799.5
4	1488.5
5	1262.0
6	1675.0
7	3130.5
8	4988.0
9	4730.0
10	2909.5
11	2035.5
12	2111.0
13	2419.5
14	2853.5
15	3442.5
16	4108.5
17	4869.5
18	5927.5
19	7239.0
20	8999.5
21	11517.5
22	14574.0
23	18617.0
24	23960.5
25	30981.0
26	42330.5
27	55715.5
28	69649.0
29	91446.0
30	115742.0
31	137637.5
32	165488.5
33	196060.5
34	226804.0
35	249753.5
36	275138.0
37	312683.5
38	329286.0
39	334165.0
40	355040.5
41	374597.5
42	385436.5
43	420848.0
44	466646.0
45	502854.5
46	565209.5
47	627056.5
48	681616.5
49	722416.5
50	765474.5
51	770102.0
52	736384.5
53	717586.0
54	669657.5
55	619331.0
56	586249.0
57	541112.0
58	499636.5
59	455665.5
60	386662.0
61	308686.0
62	248210.5
63	195402.5
64	142927.5
65	115213.0
66	97827.5
67	76678.0
68	58512.5
69	47523.5
70	40457.5
71	34777.5
72	30512.0
73	31772.0
74	25218.5
75	17731.0
76	14010.0
77	11024.5
78	8687.0
79	5861.5
80	4384.0
81	3638.0
82	3278.0
83	3092.0
84	2295.5
85	1401.0
86	1018.5
87	530.0
88	236.5
89	171.0
90	126.5
91	96.5
92	77.5
93	54.0
94	31.5
95	27.5
96	20.5
97	8.0
98	7.0
99	7.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01814662472780063
2	0.006116978925645521
3	0.006414160088025061
4	0.003925267853096417
5	0.0473013350120767
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.7766772719066477E-4
18-19	0.001662357127060549
20-21	6.160317845159204E-4
22-23	0.004965401921424806
24-25	0.007333564309136761
26-27	0.00581051085194162
28-29	0.02232263918665478
30-31	0.02028880560661981
32-33	0.013165744620835222
34-35	0.013589846904647691
36-37	0.013577464356215211
38-39	0.02104414106100114
40-41	0.030557033894254522
42-43	0.018007321057935222
44-45	0.01691146552166067
46-47	0.01827045021212544
48-49	0.01356508180778273
50-51	0.009974142762363294
52-53	0.02759141354467537
54-55	0.025891908772317375
56-57	0.013363865395754916
58-59	0.016323294471117827
60-61	0.0173758110878787
62-63	0.020768629358378442
64-65	0.011107145943935288
66-67	0.01543794225819545
68-69	0.007506919987191492
70-71	0.008884478500304983
72-73	0.010187741722823588
74-75	0.0035599826743382334
76-77	0.007305703575163679
78-79	0.005525712237994563
80-81	0.0022412412662790268
82-83	2.228858717846546E-4
84-85	0.002578665711064129
86-87	0.002396023121685037
88-89	0.0011237162702476336
90-91	7.305703575163679E-4
92-93	0.0011732464639775569
94-95	0.0032473233264180925
96-97	0.0013311239564916873
98-99	0.0018109477082503189
100	0.002074076862440536
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.6151764E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.31843131455959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4995410770298	24.537520894745796
2	14.879282081852857	10.21267240272049
3	5.349843080021559	5.507946668561755
4	2.501465016978214	3.4338542148376194
5	1.3937476375438125	2.391561628443851
6	0.8681012507401162	1.787512388856477
7	0.5757548307524426	1.3831301829242506
8	0.4307202932566053	1.182531583993102
9	0.3142431241703737	0.9705897965622231
>10	1.824853750179398	11.800290680341755
>50	0.17588362899528376	4.20242534279972
>100	0.14162598552730382	10.108913722401295
>500	0.023590564555474116	5.690033214011044
>1k	0.01990040554203412	12.782401514302666
>5k	0.0012663637481750737	2.9909219059264354
>10k+	1.809091068821534E-4	1.0176938585715583
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	33771	0.20908552155665472	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	30708	0.19012164863231037	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19659	0.12171425981707014	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.191274216240406E-6	0.0	0.0	0.0	0.0
3	6.191274216240406E-6	0.0	0.0	0.0	0.0
4	6.191274216240406E-6	0.0	0.0	0.0	0.0
5	6.191274216240406E-6	0.0	0.0	0.0	0.0
6	1.8573822648721218E-5	0.0	0.0	0.0	0.0
7	1.8573822648721218E-5	0.0	0.0	0.0	0.0
8	1.8573822648721218E-5	0.0	0.0	0.0	0.0
9	1.8573822648721218E-5	0.0	0.0	6.191274216240406E-6	0.0
10-11	1.8573822648721218E-5	0.0	0.0	2.166945975684142E-5	0.0
12-13	1.8573822648721218E-5	3.095637108120203E-6	0.0	3.095637108120203E-5	0.0
14-15	2.1669459756841422E-5	1.2382548432480811E-5	0.0	5.572146794616365E-5	0.0
16-17	3.095637108120203E-5	1.8573822648721218E-5	0.0	7.429529059488487E-5	0.0
18-19	3.405200818932223E-5	1.8573822648721218E-5	0.0	9.28691132436061E-5	1.5478185540601014E-5
20-21	4.333891951368284E-5	1.8573822648721218E-5	0.0	1.5787749251413034E-4	1.8573822648721218E-5
22-23	4.333891951368284E-5	1.8573822648721218E-5	0.0	5.634059536778769E-4	2.1669459756841422E-5
24-25	4.9530193729923246E-5	2.1669459756841422E-5	0.0	0.001448758166600255	2.4765096864961623E-5
26-27	8.358220191924547E-5	2.4765096864961623E-5	0.0	0.002659152275875254	2.4765096864961623E-5
28-29	1.021560245679667E-4	2.7860733973081824E-5	0.0	0.01085020806396131	3.095637108120203E-5
30-31	1.238254843248081E-4	3.095637108120203E-5	0.0	0.03798656295374301	3.095637108120203E-5
32-33	1.238254843248081E-4	3.095637108120203E-5	0.0	0.08898099303580712	3.095637108120203E-5
34-35	1.3001675854104852E-4	3.7147645297442436E-5	0.0	0.1546580299216853	3.095637108120203E-5
36-37	1.331123956491687E-4	4.9530193729923246E-5	0.0	0.26145441451472423	3.095637108120203E-5
38-39	1.3930366986540915E-4	6.191274216240406E-5	0.0	0.4334170558708015	3.095637108120203E-5
40-41	1.4239930697352934E-4	6.191274216240406E-5	0.0	0.645096721324061	3.095637108120203E-5
42-43	1.5478185540601015E-4	6.810401637864446E-5	0.0	0.8276681110496662	3.405200818932223E-5
44-45	1.5787749251413034E-4	6.810401637864446E-5	0.0	1.033147215375361	3.7147645297442436E-5
46-47	1.6097312962225056E-4	6.810401637864446E-5	0.0	1.2649175656603204	3.7147645297442436E-5
48-49	1.6097312962225056E-4	6.810401637864446E-5	0.0	1.5130762187956683	3.7147645297442436E-5
50-51	1.6406876673037075E-4	6.810401637864446E-5	0.0	1.7735586032584427	3.7147645297442436E-5
52-53	1.6716440383849094E-4	7.739092770300508E-5	0.0	2.0322733789324805	3.7147645297442436E-5
54-55	1.6716440383849094E-4	8.048656481112528E-5	0.0	2.2851126353753064	4.333891951368284E-5
56-57	1.6716440383849094E-4	8.048656481112528E-5	0.0	2.5690506622063074	4.333891951368284E-5
58-59	1.6716440383849094E-4	8.048656481112528E-5	0.0	2.889867013906345	4.333891951368284E-5
60-61	1.7645131516285154E-4	8.667783902736567E-5	0.0	3.218759882821468	4.333891951368284E-5
62-63	1.7954695227097176E-4	8.667783902736567E-5	0.0	3.5517049407111196	4.333891951368284E-5
64-65	1.7954695227097176E-4	8.667783902736567E-5	0.0	3.8809104689741627	4.333891951368284E-5
66-67	1.7954695227097176E-4	9.286911324360608E-5	0.0	4.231274676871207	4.333891951368284E-5
68-69	1.7954695227097176E-4	9.286911324360608E-5	0.0	4.6345123665749455	4.333891951368284E-5
70-71	1.950251378115728E-4	9.286911324360608E-5	0.0	5.08826156697188	4.9530193729923246E-5
72-73	2.0121641202781317E-4	9.286911324360608E-5	0.0	5.52438111403807	6.191274216240406E-5
74-75	2.043120491359334E-4	9.286911324360608E-5	0.0	5.948635084068837	6.191274216240406E-5
76-77	2.043120491359334E-4	9.906038745984649E-5	0.0	6.405353000452458	6.191274216240406E-5
78-79	2.043120491359334E-4	9.906038745984649E-5	0.0	6.891380408975762	6.191274216240406E-5
80-81	2.043120491359334E-4	1.052516616760869E-4	0.0	7.429104957204675	7.429529059488487E-5
82-83	2.043120491359334E-4	1.1144293589232731E-4	0.0	7.9971079319881095	9.286911324360608E-5
84-85	2.043120491359334E-4	1.1144293589232731E-4	0.0	8.54364266342673	9.286911324360608E-5
86-87	2.105033233521738E-4	1.1144293589232731E-4	0.0	9.102810689903592	9.286911324360608E-5
88	2.29077146000895E-4	1.1144293589232731E-4	0.0	9.537602208650398	9.286911324360608E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAT	6220	0.0	23.118261	1
GTACTAG	5985	0.0	22.455671	1
CATGGGG	36975	0.0	21.439379	4
TATATAC	10960	0.0	20.880322	3
ATATACT	7890	0.0	19.951387	4
GTATCAA	73895	0.0	19.63115	1
GTACATG	116300	0.0	19.617048	1
GTATAAG	6900	0.0	19.409748	1
TACATGG	114440	0.0	19.348452	2
TAGTACT	7365	0.0	19.33193	4
ACATGGG	108050	0.0	18.87921	3
TTATATA	8170	0.0	18.520494	2
GTGATCG	4460	0.0	18.119287	8
CTAACGC	2895	0.0	17.692804	3
ATGGGGG	19060	0.0	17.40531	5
CAACGCA	79835	0.0	17.121838	5
CTAGTAC	8415	0.0	17.031956	3
TCTAACG	2950	0.0	16.88506	2
AACGCAG	82490	0.0	16.80228	6
ATCAACG	81660	0.0	16.716892	3
>>END_MODULE