FastQCFastQC Report
Fri 27 May 2016
ERR765281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7386117
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA245730.3326917242172037No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT217490.2944578321735223No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG96670.13088067789882019No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG88610.11996831352657966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA22650.026.2773551
GTACTAG27700.022.4939061
GTACAAG44050.022.4841
CTAACGC12400.022.1243323
GTACAAA48850.021.13141
GTATAAG30500.021.038711
CGTTAAC7500.019.838069
TCTAACG13700.019.685542
TAGTACT36400.019.1607324
TACATGA30750.018.1457942
CTAGTAC38900.017.6909833
ATACGGG21400.017.3826543
GTATAAT22200.017.175161
ACGCCTA17250.016.711076
GTATTAG25100.016.6728061
TAGGCAT46050.016.357155
TAACGCC32750.016.1851144
CATGGGG179350.016.09954
ACGTTAA9900.015.9679188
TACGGGC22750.015.9417294
Produced by FastQC (version 0.11.5)