FastQCFastQC Report
Fri 27 May 2016
ERR765281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7386117
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT245380.3322178622407417No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA229580.31082637873188307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207200.2805262900655378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143310.19402617099079258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105920.1434041729910317No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG93500.12658884228343525No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG80900.10952981113080121No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT79980.10828423107838667No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG75080.10165016340791787No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG7400.038.9625172
TCACGCA12000.027.9016234
GTATCAA588650.024.5426181
TAACGCA6000.024.0263984
ATCACGC13750.022.9977023
AGTACTT380400.021.82165512-13
CAACGCA701950.020.351355
ATCAACG704950.020.2647423
AACGCAG727400.019.8947566
TATCAAC727150.019.8255252
TCAACGC729200.019.6418444
TACTTTT435050.019.3260614-15
CTAGGCA45150.019.2602584
TAGGCAT44250.018.8112645
GAGTACT368800.018.52971312-13
ACGCAGA771600.018.3633777
GTATAAG28500.018.2847731
TAGTACT38750.018.241064
CGCAGAG781100.018.1167148
CTAGTAC38300.017.8482973
Produced by FastQC (version 0.11.5)