##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765281_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7386117 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27592942272645 33.0 33.0 33.0 33.0 33.0 2 32.118066773109604 33.0 33.0 33.0 27.0 33.0 3 32.20192003457297 33.0 33.0 33.0 33.0 33.0 4 36.119959784011 37.0 37.0 37.0 33.0 37.0 5 36.05783498961633 37.0 37.0 37.0 33.0 37.0 6 36.062399092784474 37.0 37.0 37.0 33.0 37.0 7 35.97132133162797 37.0 37.0 37.0 33.0 37.0 8 35.937145051994165 37.0 37.0 37.0 33.0 37.0 9 35.679518480413996 37.0 37.0 37.0 33.0 37.0 10-11 35.85905652455817 37.0 37.0 37.0 33.0 37.0 12-13 35.784477080447004 37.0 37.0 37.0 33.0 37.0 14-15 37.15040013582238 37.0 37.0 40.0 33.0 40.0 16-17 37.29903655736837 37.0 37.0 40.0 33.0 40.0 18-19 37.3727422676895 37.0 37.0 40.0 33.0 40.0 20-21 37.39835362748789 37.0 37.0 40.0 33.0 40.0 22-23 37.31530288512895 37.0 37.0 40.0 33.0 40.0 24-25 37.258482366309664 37.0 37.0 40.0 33.0 40.0 26-27 37.113945866278584 37.0 37.0 40.0 33.0 40.0 28-29 37.0425867204649 37.0 37.0 40.0 33.0 40.0 30-31 36.919187375450456 37.0 37.0 40.0 33.0 40.0 32-33 36.72101572450044 37.0 37.0 40.0 33.0 40.0 34-35 36.61700491611492 37.0 37.0 40.0 33.0 40.0 36-37 36.446890226623815 37.0 37.0 40.0 33.0 40.0 38-39 36.290417617267636 37.0 37.0 40.0 33.0 40.0 40-41 36.14053940656505 37.0 37.0 40.0 33.0 40.0 42-43 35.94361008632818 37.0 37.0 40.0 33.0 40.0 44-45 35.70862606156929 37.0 37.0 40.0 27.0 40.0 46-47 35.42286312280187 37.0 37.0 40.0 27.0 40.0 48-49 35.14974776326993 37.0 33.0 40.0 27.0 40.0 50-51 34.83084894268531 37.0 33.0 40.0 27.0 40.0 52-53 34.67057034162876 37.0 33.0 40.0 27.0 40.0 54-55 34.548077494575296 37.0 33.0 40.0 27.0 40.0 56-57 34.376839278338 37.0 33.0 40.0 27.0 40.0 58-59 34.18553679829334 37.0 33.0 38.5 27.0 40.0 60-61 34.013474806857246 37.0 33.0 37.0 27.0 40.0 62-63 33.69704392714061 37.0 33.0 37.0 22.0 40.0 64-65 33.54974190092034 37.0 33.0 37.0 22.0 40.0 66-67 33.36121164882712 37.0 33.0 37.0 22.0 40.0 68-69 33.11335875128975 37.0 33.0 37.0 22.0 40.0 70-71 32.90687481663234 37.0 33.0 37.0 22.0 37.0 72-73 32.69483857350215 37.0 33.0 37.0 22.0 37.0 74-75 32.486095603413816 37.0 33.0 37.0 22.0 37.0 76-77 32.32221747908949 33.0 33.0 37.0 22.0 37.0 78-79 32.13278167675925 33.0 33.0 37.0 22.0 37.0 80-81 31.98854154354717 33.0 33.0 37.0 22.0 37.0 82-83 31.8476042960056 33.0 33.0 37.0 22.0 37.0 84-85 31.699070770203072 33.0 33.0 37.0 22.0 37.0 86-87 31.60302118149496 33.0 33.0 37.0 22.0 37.0 88-89 31.496921521822628 33.0 33.0 37.0 22.0 37.0 90-91 31.43856988726282 33.0 33.0 37.0 22.0 37.0 92-93 31.336430630600624 33.0 33.0 37.0 22.0 37.0 94-95 31.27157084026695 33.0 33.0 37.0 22.0 37.0 96-97 31.22423866288606 33.0 33.0 37.0 15.0 37.0 98-99 30.39985353603253 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13297342730697892 1101 2 0.3612228325160203 1101 3 0.23267941048056429 1101 4 0.22608847041413327 1101 5 0.05548850221008195 1101 6 0.17482771797178032 1101 7 0.16466346415966626 1101 8 0.12144495247346754 1101 9 0.36618152358197875 1101 10-11 0.3375580379608891 1101 12-13 0.28597005214714244 1101 14-15 0.5754715650062323 1101 16-17 0.41793741043247934 1101 18-19 0.2815821082120493 1101 20-21 0.33620126538862394 1101 22-23 0.20045148540072688 1101 24-25 0.38827042222742136 1101 26-27 0.25218777728250075 1101 28-29 0.1842783828468768 1101 30-31 0.27748069939972453 1101 32-33 0.3411312320808193 1101 34-35 0.3725236770665745 1101 36-37 0.32888491225617145 1101 38-39 0.12304795100035193 1101 40-41 0.0788279550472879 1101 42-43 0.2575106740019564 1101 44-45 0.3378695816143207 1101 46-47 0.38957223273988006 1101 48-49 0.45443875191577376 1101 50-51 0.42878164733679824 1101 52-53 0.45681937494516234 1101 54-55 0.4185184874530776 1101 56-57 0.5352249496201509 1101 58-59 0.5752737179568399 1101 60-61 0.3828798469358077 1101 62-63 0.45330960043223456 1101 64-65 0.3548760468864316 1101 66-67 0.38239310722759967 1101 68-69 0.24769327642023597 1101 70-71 0.29479159958153645 1101 72-73 0.3952676277887903 1101 74-75 0.2661897560092612 1101 76-77 0.24095561618553063 1101 78-79 0.5451501073939369 1101 80-81 0.5214039140731934 1101 82-83 0.44664041252970677 1101 84-85 0.457982122648513 1101 86-87 0.2858278858996961 1101 88-89 0.38425554978281085 1101 90-91 0.319943545764378 1101 92-93 0.4970249168541194 1101 94-95 0.5450091301941846 1101 96-97 0.39184735936373727 1101 98-99 0.4731252941514512 1102 1 0.1490559584703064 1102 2 0.3648520403708915 1102 3 0.207673676605026 1102 4 0.19654103269579792 1102 5 0.051766390848243304 1102 6 0.20886243270288674 1102 7 0.1701444929658038 1102 8 0.1263043833654649 1102 9 0.3552527895202715 1102 10-11 0.30837444368305 1102 12-13 0.2701084019753921 1102 14-15 0.5797559806106136 1102 16-17 0.3710471913280813 1102 18-19 0.2919087935215856 1102 20-21 0.40681813878563133 1102 22-23 0.22103060192257118 1102 24-25 0.3986304229079636 1102 26-27 0.2901569857982764 1102 28-29 0.29239786961511527 1102 30-31 0.2837906126734282 1102 32-33 0.3108322640475478 1102 34-35 0.34469501966376015 1102 36-37 0.3817745319619945 1102 38-39 0.23707871172940997 1102 40-41 0.21381639895690796 1102 42-43 0.17027253870736558 1102 44-45 0.3045461946328558 1102 46-47 0.40879336958826684 1102 48-49 0.44800011681699914 1102 50-51 0.3040494790353634 1102 52-53 0.41002508578704777 1102 54-55 0.35178776660759326 1102 56-57 0.4820730276021621 1102 58-59 0.33624099413202657 1102 60-61 0.2583758639780882 1102 62-63 0.36617388147949725 1102 64-65 0.28062442806858456 1102 66-67 0.31116775593979185 1102 68-69 0.25486893128444166 1102 70-71 0.24203095526733165 1102 72-73 0.402439421897995 1102 74-75 0.20277107217990675 1102 76-77 0.18187880940944723 1102 78-79 0.5176251000077912 1102 80-81 0.36856207939007035 1102 82-83 0.4332676896798908 1102 84-85 0.39196912597300937 1102 86-87 0.36260026690909797 1102 88-89 0.3748050350100236 1102 90-91 0.2698871721977234 1102 92-93 0.37600511805180403 1102 94-95 0.4013642355682592 1102 96-97 0.23332033391514528 1102 98-99 0.2512993091763356 1103 1 0.09243058530659454 1103 2 0.25734868764272534 1103 3 0.19780137167447265 1103 4 0.20490738225664273 1103 5 0.10787528810116953 1103 6 0.1918452959106247 1103 7 0.1700176794647632 1103 8 0.12578564702285178 1103 9 0.4364716936360651 1103 10-11 0.29191914704740185 1103 12-13 0.31213215544470785 1103 14-15 0.46831326032964427 1103 16-17 0.37937343460408357 1103 18-19 0.18042487768971682 1103 20-21 0.29238250030651614 1103 22-23 0.20238008201177848 1103 24-25 0.2973653706023853 1103 26-27 0.18095976702048944 1103 28-29 0.09202196471758128 1103 30-31 0.12399558502720254 1103 32-33 0.17293908445502382 1103 34-35 0.3326685559694482 1103 36-37 0.2934796490652971 1103 38-39 0.16804699128624634 1103 40-41 0.07733016618242061 1103 42-43 0.17293945513536357 1103 44-45 0.2471103092425153 1103 46-47 0.39854970683172297 1103 48-49 0.24618013996578725 1103 50-51 0.27464324304123977 1103 52-53 0.2522698931083269 1103 54-55 0.3197079475495883 1103 56-57 0.3713786249129214 1103 58-59 0.3832931846526222 1103 60-61 0.28669945960047016 1103 62-63 0.2788158001523726 1103 64-65 0.1526257564162563 1103 66-67 0.24963313436343526 1103 68-69 0.30088233053459135 1103 70-71 0.2680980174964631 1103 72-73 0.24867695442897286 1103 74-75 0.25346197644140744 1103 76-77 0.17446801645029808 1103 78-79 0.3714120407462502 1103 80-81 0.19830101491210783 1103 82-83 0.2796356802487132 1103 84-85 0.3438673088511912 1103 86-87 0.22730086730927113 1103 88-89 0.22273163426799414 1103 90-91 0.05517587078227848 1103 92-93 0.10987774782933357 1103 94-95 0.24706887948783773 1103 96-97 0.2003569480949139 1103 98-99 0.1720255557807313 1104 1 0.11979600719902805 1104 2 0.2720332800194356 1104 3 0.22001149996347635 1104 4 0.1627696711089328 1104 5 0.07579735205828797 1104 6 0.13350855901845904 1104 7 0.15092353240879675 1104 8 0.023539579676210565 1104 9 0.3513737551824505 1104 10-11 0.2599693510109944 1104 12-13 0.23938382537785685 1104 14-15 0.4568119789053142 1104 16-17 0.2630696110088522 1104 18-19 0.1944356268907299 1104 20-21 0.27729871403576567 1104 22-23 0.22284617320725175 1104 24-25 0.29818643889661445 1104 26-27 0.13378191598744138 1104 28-29 0.1752818762483983 1104 30-31 0.19141907580977602 1104 32-33 0.2681012022700884 1104 34-35 0.2746168945973011 1104 36-37 0.2401939353041911 1104 38-39 0.09632973449961213 1104 40-41 0.12183728651054082 1104 42-43 0.17492205006318784 1104 44-45 0.18771547901637575 1104 46-47 0.3757723636737751 1104 48-49 0.4578762149068325 1104 50-51 0.39036340354738996 1104 52-53 0.41018923022799214 1104 54-55 0.3144270659010502 1104 56-57 0.34388396700524737 1104 58-59 0.3205601867373318 1104 60-61 0.31698068542002744 1104 62-63 0.31779356434269346 1104 64-65 0.2031191472083833 1104 66-67 0.22999636590459716 1104 68-69 0.16734691556326453 1104 70-71 0.32454880235926 1104 72-73 0.30151828314490103 1104 74-75 0.2324050758561711 1104 76-77 0.22379900703725042 1104 78-79 0.41236423035256564 1104 80-81 0.29765003989865946 1104 82-83 0.2555931142771932 1104 84-85 0.21043247593897618 1104 86-87 0.25365293001152267 1104 88-89 0.1978275609186575 1104 90-91 0.23201089561168686 1104 92-93 0.24634828236566975 1104 94-95 0.3367851157811188 1104 96-97 0.28847692243973455 1104 98-99 0.2620030425473736 1105 1 0.14524539103828715 1105 2 0.28794156279013805 1105 3 0.23506525263166367 1105 4 0.1964571640609165 1105 5 -0.011387729589834805 1105 6 0.12809502830373987 1105 7 0.11960332860237344 1105 8 -0.03625506851386717 1105 9 0.2940845106188803 1105 10-11 0.18132620587888937 1105 12-13 0.18217785734605485 1105 14-15 0.401348771390559 1105 16-17 0.32360742917634155 1105 18-19 0.15730631208031554 1105 20-21 0.27340534191762345 1105 22-23 0.13161848590812752 1105 24-25 0.20516557077515785 1105 26-27 0.10062693450821314 1105 28-29 0.0446976913583228 1105 30-31 0.08741265539546816 1105 32-33 0.13093144629640108 1105 34-35 0.15229801494019313 1105 36-37 0.10128276382825163 1105 38-39 0.0736445020007892 1105 40-41 -0.02658418480074687 1105 42-43 0.13608571820804372 1105 44-45 0.1722140730814843 1105 46-47 0.2329919987913769 1105 48-49 0.2845133454167694 1105 50-51 0.15326122569234002 1105 52-53 0.05640145754842507 1105 54-55 0.14591156661715843 1105 56-57 0.17616141507909333 1105 58-59 0.15086713291831444 1105 60-61 0.09444865444493189 1105 62-63 0.11850596069717767 1105 64-65 -0.027156520476090407 1105 66-67 0.04590288161774225 1105 68-69 -0.012805257280518845 1105 70-71 0.08173846060756773 1105 72-73 0.14850132621602796 1105 74-75 0.07401439433953527 1105 76-77 0.08730391995030828 1105 78-79 0.24397413299045212 1105 80-81 0.12976320684012776 1105 82-83 0.05876353502740628 1105 84-85 0.03646256301270867 1105 86-87 0.03870349290415831 1105 88-89 0.08339496842551952 1105 90-91 0.05741587424113703 1105 92-93 -0.03977449003246036 1105 94-95 0.1916896530603296 1105 96-97 0.087543407230946 1105 98-99 0.08323027506584069 1106 1 0.09863689285005961 1106 2 0.2663634782735471 1106 3 0.1741868569689018 1106 4 0.1462497729947927 1106 5 -0.017261059269422674 1106 6 0.1303041321053655 1106 7 0.13764300189674827 1106 8 0.046962893053560606 1106 9 0.34492110777031115 1106 10-11 0.17320511788561532 1106 12-13 0.17478344761814668 1106 14-15 0.3405351618774546 1106 16-17 0.24018803135317768 1106 18-19 0.18170416434875847 1106 20-21 0.23600412116782366 1106 22-23 0.10931887133219931 1106 24-25 0.1971287641487649 1106 26-27 0.060012366046038323 1106 28-29 0.11052204823977974 1106 30-31 0.0477107060573303 1106 32-33 0.0699737963844882 1106 34-35 0.1913860621924499 1106 36-37 0.09562598300617253 1106 38-39 0.061831478437902376 1106 40-41 0.056208885713935786 1106 42-43 0.04173845233707141 1106 44-45 0.00586548976973944 1106 46-47 0.11016225180070194 1106 48-49 0.17779836607854094 1106 50-51 -0.057197932960328046 1106 52-53 0.012900065755538037 1106 54-55 0.02724094122223164 1106 56-57 0.040234495230841105 1106 58-59 0.055773920117069054 1106 60-61 0.09777206361508206 1106 62-63 0.0873453265522528 1106 64-65 -0.13328063184905403 1106 66-67 -0.06467952149374412 1106 68-69 -0.022298849970944445 1106 70-71 -0.004823459987328249 1106 72-73 0.11708634022575382 1106 74-75 0.028742918068800805 1106 76-77 0.06719076956890291 1106 78-79 0.15416356548901433 1106 80-81 0.019167003567854835 1106 82-83 0.008789938137173436 1106 84-85 -0.05011545842078036 1106 86-87 -0.04666526649263503 1106 88-89 -0.15987169563970482 1106 90-91 -0.13302197755700718 1106 92-93 -0.15475728514195097 1106 94-95 0.08672534891634953 1106 96-97 0.027825115658821176 1106 98-99 -0.08925489171435785 1107 1 0.09253371844147296 1107 2 0.18167506686894086 1107 3 0.18316377997326327 1107 4 0.12075651255256759 1107 5 0.026789206179195446 1107 6 0.11978111299769978 1107 7 0.036332248742645845 1107 8 -0.0417801832707525 1107 9 0.2827602501637614 1107 10-11 0.20708319790458773 1107 12-13 0.09254401796747658 1107 14-15 0.20685032061472697 1107 16-17 0.17568945500384103 1107 18-19 0.08531646500358647 1107 20-21 0.1691871617911005 1107 22-23 0.05396852166212085 1107 24-25 0.08519854011071715 1107 26-27 -0.04350445769082967 1107 28-29 -0.021904952842042746 1107 30-31 -0.0168486528629046 1107 32-33 -0.08304322371175488 1107 34-35 0.06844627401763148 1107 36-37 0.033273505769287226 1107 38-39 -0.05139964714959433 1107 40-41 -0.020916996812516686 1107 42-43 -0.07112800857760249 1107 44-45 -0.053188867969637954 1107 46-47 -0.020908885095771268 1107 48-49 0.11708961685338437 1107 50-51 -0.001926763621369787 1107 52-53 -0.027412371420204806 1107 54-55 0.04843436141128876 1107 56-57 0.06045146563699433 1107 58-59 0.025457936317522467 1107 60-61 0.036176389353663296 1107 62-63 0.07831743875374286 1107 64-65 -0.02924102495406089 1107 66-67 -0.049608357719478136 1107 68-69 -0.1062274907714098 1107 70-71 -0.03664123688425036 1107 72-73 0.06890874139546099 1107 74-75 0.006345532460493075 1107 76-77 0.03880626612917837 1107 78-79 0.04212614547496685 1107 80-81 0.026178591412115537 1107 82-83 -0.10433085086211591 1107 84-85 -0.13345669325768839 1107 86-87 -0.013149179511870557 1107 88-89 -0.058858854733454535 1107 90-91 -0.06107985809459393 1107 92-93 -0.06502606919169196 1107 94-95 0.09994807596090993 1107 96-97 -0.012618066318452748 1107 98-99 -0.0031297789953974586 1108 1 0.07875885487644041 1108 2 0.23058975251486657 1108 3 0.13851274185736173 1108 4 0.06330560434052757 1108 5 -0.04717985959866411 1108 6 0.023870463569771516 1108 7 0.028121334870256476 1108 8 -0.05516361292450256 1108 9 0.2300709668225025 1108 10-11 0.15956530218470277 1108 12-13 0.1629763811471534 1108 14-15 0.19932096579776015 1108 16-17 0.18332084437084717 1108 18-19 -0.06545186271696934 1108 20-21 0.14825919604744797 1108 22-23 0.04255690508143317 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80-81 -0.12627804725598324 2215 82-83 -0.09371845557316405 2215 84-85 0.018594504654874555 2215 86-87 -0.22558045325526166 2215 88-89 -0.1426324628001261 2215 90-91 -0.18536453049879142 2215 92-93 -0.06614999669079324 2215 94-95 -0.11039568716548587 2215 96-97 -0.05577532588392842 2215 98-99 0.08053630263630751 2216 1 -0.16335384890813032 2216 2 -0.027798142343151255 2216 3 -0.12137229487873924 2216 4 -0.30020079786079634 2216 5 -0.244816693806996 2216 6 -0.2706866459441386 2216 7 -0.26917013178553617 2216 8 -0.30925596409684886 2216 9 0.04417638228292731 2216 10-11 -0.24924483441782996 2216 12-13 -0.13285998452606407 2216 14-15 -0.4460675196715087 2216 16-17 -0.5999321139366955 2216 18-19 -0.5785822856629963 2216 20-21 -0.8512626724258112 2216 22-23 -0.6983816396342988 2216 24-25 -0.603767252319912 2216 26-27 -0.7156205720993398 2216 28-29 -0.8136711500275595 2216 30-31 -0.4344339058070972 2216 32-33 -0.5660038515360668 2216 34-35 -0.4872707778650138 2216 36-37 -0.48740434735169913 2216 38-39 -0.5179249609752716 2216 40-41 -0.5583137559802651 2216 42-43 -0.5924401548866172 2216 44-45 -0.4684462194154335 2216 46-47 -0.7985365775561206 2216 48-49 -0.9027747541346969 2216 50-51 -0.536978820591905 2216 52-53 -0.48878856431147 2216 54-55 -0.4766889614791623 2216 56-57 -0.5530382787666568 2216 58-59 -0.5388113931346297 2216 60-61 -0.4676698323541473 2216 62-63 -0.5337099195167312 2216 64-65 -0.6209870765711969 2216 66-67 -0.5613562246866479 2216 68-69 -0.511440748659922 2216 70-71 -0.49058107815238117 2216 72-73 -0.37911602879692907 2216 74-75 -0.43748447006576185 2216 76-77 -0.3501247095340787 2216 78-79 -0.3918874978169242 2216 80-81 -0.30175940624221553 2216 82-83 -0.2618662908621765 2216 84-85 -0.29690055138168603 2216 86-87 -0.45057841077602845 2216 88-89 -0.37473533882757337 2216 90-91 -0.3562230547437437 2216 92-93 -0.3137209210799803 2216 94-95 -0.31999441463270983 2216 96-97 -0.3327335739166699 2216 98-99 -0.3601321735305305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 11.0 9 46.0 10 105.0 11 201.0 12 325.0 13 790.0 14 2581.0 15 6573.0 16 13418.0 17 22488.0 18 34517.0 19 46062.0 20 57026.0 21 70608.0 22 80779.0 23 74098.0 24 59908.0 25 57220.0 26 67631.0 27 86950.0 28 114598.0 29 150739.0 30 197888.0 31 256972.0 32 339052.0 33 446103.0 34 588039.0 35 820933.0 36 1310487.0 37 1856431.0 38 622955.0 39 583.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.95198906099762 18.588176199867082 13.062823132836431 25.397011606298868 2 15.701383829251855 21.271728832671386 37.2293843589369 25.797502979139857 3 18.113834373324984 26.027573080686373 29.894002491430882 25.964590054557764 4 13.31089123012809 17.38190716448169 36.0602330019955 33.24696860339472 5 13.807108660803259 37.25158138728825 33.503111325332526 15.438198626575971 6 31.502655590210658 36.844095483459036 17.140454179103852 14.512794747226451 7 26.655832827993382 32.16556141745385 22.43540144300449 18.743204311548272 8 27.21017010697231 32.42044500513599 20.169650710921587 20.199734176970118 9 27.11911313084212 13.804161010544286 19.79591383576218 39.280812022851414 10-11 25.357126078560626 25.060840493049323 27.302268566826115 22.27976486156393 12-13 26.764821082579655 22.931853096830174 27.289982002722134 23.013343817868034 14-15 24.23859631735515 23.341063587995773 25.857603686043213 26.56273640860587 16-17 23.100300597356263 25.903337264980397 26.691275622585444 24.3050865150779 18-19 23.46468381153453 24.54015418385601 29.46200960531765 22.533152399291804 20-21 24.416232507554376 24.115336921957777 28.816284659449614 22.652145911038236 22-23 23.83695654969993 23.820743700648123 28.55201183517672 23.790287914475226 24-25 23.53891090541891 24.338823768970894 28.531040057989877 23.59122526762032 26-27 23.789746357930696 24.065446025293127 28.480790380114478 23.6640172366617 28-29 23.319160798563036 23.923517593885933 29.140446868090503 23.61687473946053 30-31 23.897197945821873 23.98951979775029 28.807179740044734 23.306102516383103 32-33 23.449865470584882 24.1120808132338 28.40013230226383 24.037921413917488 34-35 23.628558821908722 24.217616644848707 28.758629195827794 23.395195337414773 36-37 23.9739365081815 24.000188461677496 28.160202444667476 23.86567258547353 38-39 24.102617112618173 23.73835264185498 28.21748558816493 23.941544657361913 40-41 23.842419501342857 24.019386641181015 28.277625442434772 23.860568415041353 42-43 24.389344225118556 23.923240046156867 27.7484705427764 23.938945185948178 44-45 24.103195575185595 24.237082698551905 27.32118456730556 24.338537158956942 46-47 23.98212078146068 24.323545104958395 27.25072592270066 24.44360819088027 48-49 24.656101440039468 24.640369222420926 26.409268902726563 24.29426043481304 50-51 23.9350730566548 25.08787770353489 26.046669718337796 24.930379521472513 52-53 23.88122445412494 25.09628390465089 25.69794111222033 25.324550529003844 54-55 24.07458479198204 25.146616280245766 25.684524087555072 25.09427484021713 56-57 24.3200439748052 24.60016673329003 25.541739340289922 25.53804995161485 58-59 23.83930555121182 24.659330470936215 26.284440119212842 25.216923858639117 60-61 24.009882323824545 24.80037887295855 26.017933374193774 25.171805429023124 62-63 23.641339556359586 24.842153190912086 25.920229804104107 25.59627744862422 64-65 23.41456275333846 24.65571558100149 26.143222480770344 25.786499184889706 66-67 24.11889088678124 24.494758206510944 25.995167690953174 25.391183215754637 68-69 23.986609276173997 24.265731619453188 26.01629824823516 25.731360856137652 70-71 23.525302291198727 24.378872989633216 26.054522758320033 26.041301960848028 72-73 24.076879637839475 24.304448467306976 26.055896487965192 25.562775406888356 74-75 24.143416628791556 24.234723062198988 26.071222538175338 25.550637770834122 76-77 23.748967150127733 24.517097413972728 26.11495999860278 25.618975437296754 78-79 23.90246702697975 24.657184365745465 25.966248792576142 25.474099814698643 80-81 23.456668940772563 24.58365110567173 26.143963494440133 25.815716459115578 82-83 23.498436323172243 24.618253407033762 25.792219375891285 26.091090893902706 84-85 23.480666499054916 24.696968650780917 25.524203042004345 26.29816180815982 86-87 23.33137289864214 24.435241142267312 25.74699940442319 26.48638655466736 88-89 23.432434119307885 24.100362883501575 25.97219215455157 26.495010842638965 90-91 23.560954350290473 24.302022733805273 25.654016270848473 26.483006645055784 92-93 23.223953917984698 24.415084737122967 25.680869818279696 26.680091526612642 94-95 22.68980542715707 24.42990372291001 26.125729531387233 26.75456131854569 96-97 23.277574512976816 24.320351373716235 25.699869051255224 26.702205062051725 98-99 23.059484435461826 24.25531574980467 25.75662557200218 26.92857424273133 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 128.0 1 110.5 2 260.0 3 466.5 4 363.5 5 309.0 6 625.0 7 1625.5 8 2921.0 9 2898.0 10 1836.5 11 1253.0 12 1253.5 13 1489.0 14 1838.0 15 2317.0 16 2847.5 17 3424.5 18 4225.5 19 5332.5 20 6757.5 21 8519.0 22 10878.0 23 13857.0 24 17448.5 25 21932.5 26 27450.0 27 33228.0 28 40204.0 29 48725.0 30 56371.5 31 64297.0 32 72791.5 33 81311.0 34 89155.5 35 96862.5 36 103719.5 37 113593.0 38 128126.0 39 134851.5 40 141228.0 41 153248.5 42 168672.0 43 192514.5 44 219002.0 45 247217.5 46 280351.0 47 313421.0 48 337838.0 49 361646.0 50 391323.0 51 374420.5 52 335455.5 53 324141.5 54 305703.5 55 289352.0 56 267873.0 57 242691.0 58 230153.0 59 204906.5 60 169523.5 61 134929.5 62 109361.5 63 86340.5 64 63398.5 65 48134.5 66 35656.5 67 27006.0 68 21525.0 69 18522.0 70 16252.0 71 14244.0 72 13976.5 73 11888.5 74 8207.5 75 6453.5 76 4962.5 77 3294.0 78 1941.5 79 1271.0 80 863.0 81 626.5 82 416.5 83 254.5 84 162.0 85 98.0 86 58.0 87 24.0 88 12.5 89 8.5 90 5.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055387695591607874 2 0.008069192513468174 3 0.0 4 0.0 5 0.0026671659818007214 6 0.0 7 0.0 8 0.0 9 0.004264757788158514 10-11 0.0 12-13 0.0 14-15 0.0027416300066733304 16-17 0.0043798385538707275 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.024816557874726E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.3693098823102855E-4 54-55 0.0 56-57 9.409544961175133E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.001468972127032377 70-71 3.6555066755644406E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.483260013346661E-4 80-81 8.664904712449045E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.249339267168392E-4 92-93 9.274155825043118E-4 94-95 5.415565445280652E-4 96-97 2.8431718587723425E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7386117.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.24597894697634 #Duplication Level Percentage of deduplicated Percentage of total 1 70.72379743467235 27.756246651713706 2 15.463779928125726 12.137823629997952 3 5.6477558634210965 6.649551231404597 4 2.620154784033634 4.1132215796801335 5 1.383770253235312 2.7153709112962585 6 0.8540960947799812 2.0111902412657905 7 0.5843535972662598 1.6053470283131068 8 0.4176056410413771 1.3111473757158754 9 0.2996618803383553 1.058447146227339 >10 1.7071021206719847 12.29623332903493 >50 0.14673849053730204 4.0258599850439225 >100 0.12151496897207063 10.022371981555732 >500 0.01917723283431717 5.186005871295616 >1k 0.009970598643334064 6.886261295979011 >5k 3.4740761803604074E-4 0.955031824637235 >10k+ 1.7370380901802037E-4 1.2698899168387547 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 24538 0.3322178622407417 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 22958 0.31082637873188307 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20720 0.2805262900655378 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14331 0.19402617099079258 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10592 0.1434041729910317 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9350 0.12658884228343525 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 8090 0.10952981113080121 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 7998 0.10828423107838667 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 7508 0.10165016340791787 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.7077827226403264E-5 0.0 4 0.0 0.0 0.0 5.415565445280653E-5 0.0 5 0.0 0.0 0.0 6.769456806600817E-5 0.0 6 0.0 0.0 0.0 6.769456806600817E-5 0.0 7 0.0 0.0 0.0 6.769456806600817E-5 0.0 8 0.0 0.0 0.0 6.769456806600817E-5 0.0 9 0.0 0.0 0.0 9.477239529241143E-5 0.0 10-11 0.0 0.0 0.0 1.0831130890561306E-4 0.0 12-13 1.3538913613201632E-5 0.0 0.0 1.3538913613201633E-4 0.0 14-15 2.7077827226403264E-5 2.7077827226403264E-5 0.0 1.6923642016502042E-4 2.030837041980245E-5 16-17 2.7077827226403264E-5 5.415565445280653E-5 0.0 1.8954479058482285E-4 2.7077827226403264E-5 18-19 2.7077827226403264E-5 5.415565445280653E-5 0.0 2.3693098823102858E-4 2.7077827226403264E-5 20-21 4.06167408396049E-5 5.415565445280653E-5 0.0 5.754038285610693E-4 2.7077827226403264E-5 22-23 4.7386197646205714E-5 5.415565445280653E-5 0.0 0.0021662261781122613 2.7077827226403264E-5 24-25 8.80029384858106E-5 6.769456806600817E-5 0.0 0.005808193940063501 5.415565445280653E-5 26-27 1.2185022251881469E-4 1.0831130890561306E-4 0.0 0.009578781381340155 5.415565445280653E-5 28-29 1.2185022251881469E-4 1.0831130890561306E-4 0.0 0.023165081192187992 5.415565445280653E-5 30-31 1.4215859293861713E-4 1.0831130890561306E-4 0.0 0.060898033432180934 5.415565445280653E-5 32-33 1.4892804974521795E-4 1.0831130890561306E-4 0.0 0.12162683044419687 5.415565445280653E-5 34-35 1.4892804974521795E-4 1.0831130890561306E-4 0.0 0.19939435023842705 5.415565445280653E-5 36-37 1.4892804974521795E-4 1.3538913613201633E-4 0.0 0.3185570984050212 5.415565445280653E-5 38-39 1.4892804974521795E-4 1.3538913613201633E-4 0.0 0.49786647029826364 5.415565445280653E-5 40-41 1.4892804974521795E-4 1.3538913613201633E-4 0.0 0.7143130822325181 5.415565445280653E-5 42-43 1.4892804974521795E-4 1.3538913613201633E-4 0.0 0.9060850241067127 6.0925111259407344E-5 44-45 1.4892804974521795E-4 1.3538913613201633E-4 0.0 1.1239667608839665 6.769456806600817E-5 46-47 1.4892804974521795E-4 1.3538913613201633E-4 0.0 1.3592256932837645 6.769456806600817E-5 48-49 1.5569750655181877E-4 1.4215859293861713E-4 0.0 1.6105553161424333 8.12334816792098E-5 50-51 1.760058769716212E-4 1.4892804974521795E-4 0.0 1.8964836327396384 8.12334816792098E-5 52-53 1.760058769716212E-4 1.4892804974521795E-4 0.0 2.1770234617187896 8.80029384858106E-5 54-55 1.760058769716212E-4 1.4892804974521795E-4 0.0 2.4443966972091022 9.477239529241143E-5 56-57 1.760058769716212E-4 1.4892804974521795E-4 0.0 2.7462738540426583 9.477239529241143E-5 58-59 1.760058769716212E-4 1.4892804974521795E-4 0.0 3.0758583975856326 9.477239529241143E-5 60-61 1.760058769716212E-4 1.4892804974521795E-4 0.0 3.4034594902842725 9.477239529241143E-5 62-63 1.760058769716212E-4 1.4892804974521795E-4 0.0 3.7373358694426315 9.477239529241143E-5 64-65 1.760058769716212E-4 1.760058769716212E-4 0.0 4.087154319380535 9.477239529241143E-5 66-67 1.760058769716212E-4 1.760058769716212E-4 0.0 4.463698584790899 9.477239529241143E-5 68-69 1.8954479058482285E-4 1.760058769716212E-4 0.0 4.867266521773213 9.477239529241143E-5 70-71 1.8954479058482285E-4 1.760058769716212E-4 0.0 5.3236700691310475 9.477239529241143E-5 72-73 1.8954479058482285E-4 1.760058769716212E-4 0.0 5.7646866411674775 1.0831130890561306E-4 74-75 1.8954479058482285E-4 1.760058769716212E-4 0.0 6.199637779905192 1.0831130890561306E-4 76-77 1.8954479058482285E-4 1.8954479058482285E-4 0.0 6.660204543199086 1.2185022251881469E-4 78-79 1.8954479058482285E-4 1.8954479058482285E-4 0.0 7.135562569615401 1.4892804974521795E-4 80-81 1.8954479058482285E-4 1.8954479058482285E-4 0.0 7.647834443998112 1.4892804974521795E-4 82-83 1.8954479058482285E-4 1.8954479058482285E-4 0.0 8.207282662866023 1.4892804974521795E-4 84-85 1.8954479058482285E-4 1.8954479058482285E-4 0.0 8.745921571510443 1.4892804974521795E-4 86-87 1.8954479058482285E-4 1.8954479058482285E-4 0.0 9.29201365209893 1.4892804974521795E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 740 0.0 38.962517 2 TCACGCA 1200 0.0 27.901623 4 GTATCAA 58865 0.0 24.542618 1 TAACGCA 600 0.0 24.026398 4 ATCACGC 1375 0.0 22.997702 3 AGTACTT 38040 0.0 21.821655 12-13 CAACGCA 70195 0.0 20.35135 5 ATCAACG 70495 0.0 20.264742 3 AACGCAG 72740 0.0 19.894756 6 TATCAAC 72715 0.0 19.825525 2 TCAACGC 72920 0.0 19.641844 4 TACTTTT 43505 0.0 19.32606 14-15 CTAGGCA 4515 0.0 19.260258 4 TAGGCAT 4425 0.0 18.811264 5 GAGTACT 36880 0.0 18.529713 12-13 ACGCAGA 77160 0.0 18.363377 7 GTATAAG 2850 0.0 18.284773 1 TAGTACT 3875 0.0 18.24106 4 CGCAGAG 78110 0.0 18.116714 8 CTAGTAC 3830 0.0 17.848297 3 >>END_MODULE