##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765278_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7097011 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10288528508692 33.0 31.0 34.0 30.0 34.0 2 32.111027304311634 34.0 31.0 34.0 30.0 34.0 3 32.13418944961477 34.0 31.0 34.0 30.0 34.0 4 35.61183884314115 37.0 35.0 37.0 33.0 37.0 5 35.63678032907093 37.0 35.0 37.0 35.0 37.0 6 35.74817609836028 37.0 36.0 37.0 35.0 37.0 7 35.70369469626016 37.0 36.0 37.0 35.0 37.0 8 35.74301082526151 37.0 36.0 37.0 35.0 37.0 9 37.415054027674465 39.0 38.0 39.0 35.0 39.0 10-11 37.336390911610536 39.0 37.5 39.0 34.5 39.0 12-13 37.327016331241424 39.0 37.5 39.0 34.5 39.0 14-15 38.681989713697774 40.5 38.0 41.0 34.5 41.0 16-17 38.62250065555767 40.0 38.0 41.0 34.0 41.0 18-19 38.59151155324403 40.0 38.0 41.0 34.0 41.0 20-21 38.58359357199812 40.0 38.5 41.0 34.0 41.0 22-23 38.50172614921972 40.0 38.0 41.0 34.0 41.0 24-25 38.433520675112376 40.0 38.0 41.0 34.0 41.0 26-27 38.30369334357802 40.0 38.0 41.0 34.0 41.0 28-29 38.16764043341627 40.0 38.0 41.0 34.0 41.0 30-31 37.95688551137937 40.0 38.0 41.0 33.0 41.0 32-33 37.679252927746624 40.0 37.0 41.0 33.0 41.0 34-35 37.55063448541929 40.0 37.0 41.0 33.0 41.0 36-37 37.42517850120283 40.0 37.0 41.0 32.0 41.0 38-39 37.24308275695219 40.0 37.0 41.0 31.5 41.0 40-41 36.99563013217818 40.0 36.5 41.0 31.0 41.0 42-43 36.75282946581314 40.0 36.0 41.0 31.0 41.0 44-45 36.42952399256532 39.0 35.5 41.0 30.0 41.0 46-47 36.04366887693988 39.0 35.0 41.0 29.5 41.0 48-49 35.678712841222875 39.0 35.0 41.0 28.0 41.0 50-51 34.4511742337725 38.0 34.0 39.5 25.5 40.5 52-53 34.422647999277444 38.0 34.0 39.5 26.0 40.0 54-55 35.26952776316678 39.0 35.0 40.0 27.5 41.0 56-57 35.297290436776834 39.0 35.0 41.0 27.0 41.0 58-59 35.09925650390002 38.0 35.0 41.0 27.0 41.0 60-61 34.748183777649494 38.0 35.0 40.0 26.0 41.0 62-63 34.28212581324729 37.0 34.0 40.0 26.0 41.0 64-65 34.009907480769016 36.5 34.0 39.5 26.0 41.0 66-67 33.71982972831802 36.0 34.0 39.0 26.0 41.0 68-69 33.49058413464485 36.0 34.0 39.0 25.0 41.0 70-71 33.10056092628291 35.0 34.0 38.5 24.0 40.5 72-73 32.709820091866845 35.0 34.0 37.0 24.0 39.5 74-75 32.333406979360745 35.0 33.0 37.0 22.5 39.0 76-77 31.95546146117006 35.0 33.0 36.0 21.0 39.0 78-79 31.654576201164126 35.0 33.0 36.0 20.0 37.0 80-81 31.36872755305015 35.0 33.0 35.5 20.0 37.0 82-83 31.13960045996829 35.0 33.0 35.0 18.5 36.5 84-85 30.906995635204737 35.0 33.0 35.0 17.5 36.0 86-87 30.652430015960242 35.0 32.0 35.0 13.0 36.0 88-89 30.45558292075354 35.0 32.0 35.0 7.5 36.0 90-91 30.220154865196065 35.0 32.0 35.0 2.0 35.0 92-93 29.929078593791104 35.0 31.0 35.0 2.0 35.0 94-95 29.666830021821863 35.0 31.0 35.0 2.0 35.0 96-97 29.411037900321702 35.0 31.0 35.0 2.0 35.0 98-99 29.093211282890785 35.0 31.0 35.0 2.0 35.0 100 28.526421475181593 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4576554320541746 1101 2 0.5946314124839631 1101 3 0.6447426172124366 1101 4 0.44555345289172266 1101 5 0.48799313678969725 1101 6 0.4638590690568023 1101 7 0.5317296572160188 1101 8 0.5057099038086008 1101 9 0.6238592813720842 1101 10-11 0.6644158877295823 1101 12-13 0.6238603848140087 1101 14-15 0.8491516951757987 1101 16-17 0.8323950131639108 1101 18-19 0.8184937134797465 1101 20-21 0.7770132812020236 1101 22-23 0.744668080182997 1101 24-25 0.8219263718307914 1101 26-27 0.8508658467721375 1101 28-29 0.8646324775851539 1101 30-31 0.8996845448103556 1101 32-33 0.9566002264775477 1101 34-35 1.0183104885863656 1101 36-37 1.063572755047879 1101 38-39 1.0021844006962723 1101 40-41 1.0160683666295753 1101 42-43 1.0030303500979656 1101 44-45 1.0308358869956251 1101 46-47 1.1028555823514026 1101 48-49 1.2266367721080869 1101 50-51 1.1215150063757164 1101 52-53 1.162639901329186 1101 54-55 1.3408419395725204 1101 56-57 1.3238802424555942 1101 58-59 1.3110174292811507 1101 60-61 1.268457641891409 1101 62-63 1.2468409680227452 1101 64-65 1.2259399974270977 1101 66-67 1.125595888524579 1101 68-69 1.1793799672487282 1101 70-71 1.1129113923852643 1101 72-73 1.0679275073173997 1101 74-75 1.0324344205961893 1101 76-77 0.9812846845410377 1101 78-79 1.0569477942930305 1101 80-81 1.089675050125937 1101 82-83 1.1073570310298102 1101 84-85 1.0553978178373313 1101 86-87 1.043403600914388 1101 88-89 1.102902977601559 1101 90-91 1.1061516232068662 1101 92-93 1.0770642900295435 1101 94-95 1.045073898095744 1101 96-97 1.1043379655400827 1101 98-99 1.0224567178398907 1101 100 1.076532208619259 1102 1 0.45613690214024416 1102 2 0.5838183925847531 1102 3 0.6344340189150373 1102 4 0.4644277170171591 1102 5 0.49035154228729283 1102 6 0.47208141349947397 1102 7 0.49591759956078363 1102 8 0.46083120827736224 1102 9 0.558900127720726 1102 10-11 0.5327542243280874 1102 12-13 0.620370949284478 1102 14-15 0.7649569820225395 1102 16-17 0.7847197582781931 1102 18-19 0.805132502102957 1102 20-21 0.8034277556267924 1102 22-23 0.8039533834617245 1102 24-25 0.7775910112894806 1102 26-27 0.7526116901807924 1102 28-29 0.7911439691988633 1102 30-31 0.8742854193267249 1102 32-33 0.8795123204680877 1102 34-35 0.8551755800455325 1102 36-37 0.8646718131623388 1102 38-39 0.8781954830839638 1102 40-41 0.9389616696294283 1102 42-43 0.862449653413961 1102 44-45 0.8490713905784375 1102 46-47 0.8804777032145665 1102 48-49 1.0036421490547482 1102 50-51 0.9974551610948623 1102 52-53 0.9776652016884952 1102 54-55 1.1280551515332036 1102 56-57 1.0658830873087055 1102 58-59 1.0787305856858609 1102 60-61 1.054313000525184 1102 62-63 0.9690600769049453 1102 64-65 0.982126832767662 1102 66-67 1.0415111072057144 1102 68-69 0.9656530547495095 1102 70-71 0.8811295755766579 1102 72-73 0.908005797665254 1102 74-75 0.9739538619185595 1102 76-77 0.929762208821657 1102 78-79 0.8850079903390409 1102 80-81 0.8643370301601614 1102 82-83 0.8418543729367549 1102 84-85 0.8637146134850298 1102 86-87 0.8776217649819458 1102 88-89 0.7595534208752781 1102 90-91 0.7707416685448969 1102 92-93 0.7982734966345824 1102 94-95 0.8168837628030303 1102 96-97 0.9145497463595476 1102 98-99 1.0086492431287546 1102 100 0.9849640438617868 1103 1 0.5112952592027469 1103 2 0.5467699014496787 1103 3 0.6157260287662538 1103 4 0.5296669570199484 1103 5 0.5103296205616914 1103 6 0.5042389372446863 1103 7 0.5546977474885608 1103 8 0.5202442921905757 1103 9 0.6226316935812264 1103 10-11 0.6352471511587723 1103 12-13 0.6662431685737431 1103 14-15 0.8267250732291345 1103 16-17 0.8595869864379111 1103 18-19 0.8557307174844198 1103 20-21 0.7828055742544251 1103 22-23 0.8080019745310665 1103 24-25 0.7500497496632974 1103 26-27 0.8412043536841338 1103 28-29 0.8909980389675738 1103 30-31 0.9712275220643889 1103 32-33 0.9730332700281821 1103 34-35 1.0048173094483843 1103 36-37 0.9871384596750161 1103 38-39 1.0044399181870247 1103 40-41 1.0414726575553885 1103 42-43 1.0529809048632686 1103 44-45 1.116458784207417 1103 46-47 1.0720789748524098 1103 48-49 0.996008430573788 1103 50-51 0.972824299685449 1103 52-53 1.0925733652921679 1103 54-55 1.1374715912776807 1103 56-57 1.1825213841918654 1103 58-59 1.1412724267484577 1103 60-61 1.1498659372535798 1103 62-63 1.1781051759726182 1103 64-65 1.1681447190634415 1103 66-67 1.1451862381258664 1103 68-69 1.109899416521337 1103 70-71 1.1272898550917674 1103 72-73 1.1137967089802672 1103 74-75 1.1021956433510383 1103 76-77 1.1118039478419064 1103 78-79 1.0434619959082454 1103 80-81 1.0607090518029345 1103 82-83 1.0558092696529293 1103 84-85 1.1227250774113315 1103 86-87 1.0759807634925025 1103 88-89 1.0773030676583701 1103 90-91 1.0332628789368279 1103 92-93 1.057104147259043 1103 94-95 1.1073861392899218 1103 96-97 1.0816764961634675 1103 98-99 1.15345846155736 1103 100 1.2325375613506822 1104 1 0.38701568217000926 1104 2 0.37549486851539626 1104 3 0.39044209788685436 1104 4 0.3538979402368625 1104 5 0.402403072038247 1104 6 0.3767294687993825 1104 7 0.45980227216377756 1104 8 0.43803408908149066 1104 9 0.5401771434965781 1104 10-11 0.5288693837940386 1104 12-13 0.5151231887992225 1104 14-15 0.5928085443606221 1104 16-17 0.5843247854963138 1104 18-19 0.5763976489793876 1104 20-21 0.5654913966080457 1104 22-23 0.5092391622627233 1104 24-25 0.5879981771414862 1104 26-27 0.6096495904133832 1104 28-29 0.5768345798227585 1104 30-31 0.5407821797984766 1104 32-33 0.5794191771887824 1104 34-35 0.5390502288650225 1104 36-37 0.5643767274733875 1104 38-39 0.4857273185076991 1104 40-41 0.5038504955500755 1104 42-43 0.5004995837810284 1104 44-45 0.49418988985298284 1104 46-47 0.4135492802136653 1104 48-49 0.4611155752936398 1104 50-51 0.3393540765897214 1104 52-53 0.37652659724021476 1104 54-55 0.49826618028841807 1104 56-57 0.5118706404288602 1104 58-59 0.5601332574689764 1104 60-61 0.49561328431803986 1104 62-63 0.45668695918091373 1104 64-65 0.4483107259898702 1104 66-67 0.43243611291590867 1104 68-69 0.40741661037289845 1104 70-71 0.38075847982758404 1104 72-73 0.39186058172876415 1104 74-75 0.3970038927253938 1104 76-77 0.35076914044172725 1104 78-79 0.32193507839433266 1104 80-81 0.3896502720660173 1104 82-83 0.4160368542626607 1104 84-85 0.33428480213006395 1104 86-87 0.3502936958266609 1104 88-89 0.3965567315616525 1104 90-91 0.3078298800717043 1104 92-93 0.28685322822760995 1104 94-95 0.24138565324970074 1104 96-97 0.2634165252707632 1104 98-99 0.25280946721255404 1104 100 0.24511056051996505 1105 1 0.24167484290280328 1105 2 0.3628635138364942 1105 3 0.3882865117086851 1105 4 0.3378752163117724 1105 5 0.416326984939964 1105 6 0.37848069730884504 1105 7 0.37495086847673775 1105 8 0.3817366208338484 1105 9 0.45725723513577776 1105 10-11 0.44535974346396046 1105 12-13 0.4717415894399224 1105 14-15 0.5518768043311795 1105 16-17 0.526825736081129 1105 18-19 0.5947670336999664 1105 20-21 0.5433439787557717 1105 22-23 0.45713268089313885 1105 24-25 0.44570246988130435 1105 26-27 0.4519207315885154 1105 28-29 0.45340687538956814 1105 30-31 0.4173607685149676 1105 32-33 0.4626797227975601 1105 34-35 0.4720741292442767 1105 36-37 0.483535747431155 1105 38-39 0.47789921032791227 1105 40-41 0.5033461999281812 1105 42-43 0.5177567454993834 1105 44-45 0.5149000526421759 1105 46-47 0.39425026503686667 1105 48-49 0.4357122277210621 1105 50-51 0.38429040313961593 1105 52-53 0.3767841831533332 1105 54-55 0.44134619184956847 1105 56-57 0.5053274520474318 1105 58-59 0.42975214706004294 1105 60-61 0.41528731649318473 1105 62-63 0.5156971853667471 1105 64-65 0.4714346309020172 1105 66-67 0.4074997808937084 1105 68-69 0.409039695761912 1105 70-71 0.39349141415090827 1105 72-73 0.3821186264033827 1105 74-75 0.4071343588389311 1105 76-77 0.417494608535776 1105 78-79 0.49179954675132365 1105 80-81 0.3852594320828473 1105 82-83 0.4363393303981731 1105 84-85 0.4670857426002435 1105 86-87 0.41835691425468013 1105 88-89 0.4725237811771237 1105 90-91 0.5479088207941594 1105 92-93 0.5488736229576716 1105 94-95 0.5283099337990436 1105 96-97 0.440162119587157 1105 98-99 0.4397113386767053 1105 100 0.5745765101567066 1106 1 0.16188118621435876 1106 2 0.2422005268340044 1106 3 0.3227019377357223 1106 4 0.235466012260801 1106 5 0.3585350278308965 1106 6 0.301054139159902 1106 7 0.2495344857415418 1106 8 0.27473219249996106 1106 9 0.27846422500707746 1106 10-11 0.3684917282470863 1106 12-13 0.3592110354720006 1106 14-15 0.45592889127575376 1106 16-17 0.42229599299644605 1106 18-19 0.4235322895760163 1106 20-21 0.38712084228635746 1106 22-23 0.48685474251372796 1106 24-25 0.5295150836386284 1106 26-27 0.5304245781713774 1106 28-29 0.47798237194206195 1106 30-31 0.4812868651961679 1106 32-33 0.4807072480240606 1106 34-35 0.48378100156890724 1106 36-37 0.5520654267041749 1106 38-39 0.578588374111348 1106 40-41 0.5569032416316091 1106 42-43 0.4996444704147862 1106 44-45 0.5635352171678534 1106 46-47 0.4992807005247002 1106 48-49 0.559943480744856 1106 50-51 0.5363244039765505 1106 52-53 0.5635116044016257 1106 54-55 0.574042672250549 1106 56-57 0.5437914774404007 1106 58-59 0.5712596957797516 1106 60-61 0.6049911124509961 1106 62-63 0.5665861657680438 1106 64-65 0.5416664882697262 1106 66-67 0.4962930769868805 1106 68-69 0.4488747171695735 1106 70-71 0.46824170118669173 1106 72-73 0.4826917921332523 1106 74-75 0.5310190920737483 1106 76-77 0.5938197749244623 1106 78-79 0.5160857482312906 1106 80-81 0.6183427876307555 1106 82-83 0.6115267485042502 1106 84-85 0.6120374237255817 1106 86-87 0.6630410297892553 1106 88-89 0.5928321976844657 1106 90-91 0.6216973401350074 1106 92-93 0.5356577258444659 1106 94-95 0.5325729789448133 1106 96-97 0.504383970998397 1106 98-99 0.6241121376631504 1106 100 0.6607373526165432 1107 1 0.12793697456153552 1107 2 0.2378725696139341 1107 3 0.2740345803773394 1107 4 0.2711659806979796 1107 5 0.31043011349211724 1107 6 0.21382990000120827 1107 7 0.27473939491018484 1107 8 0.2580885235169674 1107 9 0.3052738871480756 1107 10-11 0.34633426843853243 1107 12-13 0.3492043585417832 1107 14-15 0.3835865355306254 1107 16-17 0.35710095479618076 1107 18-19 0.35051579589589466 1107 20-21 0.33537905827709835 1107 22-23 0.35676262409872805 1107 24-25 0.3876954862831141 1107 26-27 0.37259178674868565 1107 28-29 0.34927777739683563 1107 30-31 0.37674064768738447 1107 32-33 0.33623608137884275 1107 34-35 0.32719207842013986 1107 36-37 0.33730544654273587 1107 38-39 0.41457731622337946 1107 40-41 0.3532954583117416 1107 42-43 0.36753598727883485 1107 44-45 0.39998921813656096 1107 46-47 0.3375487974303013 1107 48-49 0.35119339749382306 1107 50-51 0.3247432580620284 1107 52-53 0.4125132122197357 1107 54-55 0.4688507251728069 1107 56-57 0.4166051420857215 1107 58-59 0.3592834208556184 1107 60-61 0.4028327850657618 1107 62-63 0.4758552238893259 1107 64-65 0.439087268460689 1107 66-67 0.3957968633176492 1107 68-69 0.32166180035343217 1107 70-71 0.3580942247214338 1107 72-73 0.3340316957055691 1107 74-75 0.28619753934167136 1107 76-77 0.3307205291660722 1107 78-79 0.337698463665717 1107 80-81 0.408523102502631 1107 82-83 0.40088963522619636 1107 84-85 0.429865817991967 1107 86-87 0.37552682839734075 1107 88-89 0.4158636263094273 1107 90-91 0.4182968800351823 1107 92-93 0.4718380524491259 1107 94-95 0.3632968531387277 1107 96-97 0.43291386068970183 1107 98-99 0.46697722378536 1107 100 0.4696958714694972 1108 1 0.15408373413288246 1108 2 0.14596928842799173 1108 3 0.21595080520504695 1108 4 0.2558421837853544 1108 5 0.2685500771565259 1108 6 0.24391941116040528 1108 7 0.34731214880111594 1108 8 0.2895284775892222 1108 9 0.37584963870238397 1108 10-11 0.33178869533913513 1108 12-13 0.3740423548162255 1108 14-15 0.38397970935128 1108 16-17 0.3637765232325947 1108 18-19 0.41994479371633986 1108 20-21 0.3896328911867428 1108 22-23 0.3831020010931496 1108 24-25 0.45107228083187323 1108 26-27 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48-49 -1.299019541743668 2316 50-51 -1.2630152110777715 2316 52-53 -1.2860010441303729 2316 54-55 -1.5198896911265507 2316 56-57 -1.419448593442965 2316 58-59 -1.417351665656966 2316 60-61 -1.385697896727244 2316 62-63 -1.3859546009872616 2316 64-65 -1.4198221870427332 2316 66-67 -1.306219072008652 2316 68-69 -1.3014308544825113 2316 70-71 -1.3179058467074043 2316 72-73 -1.2404491498077341 2316 74-75 -1.1685922581974424 2316 76-77 -1.1796454288085805 2316 78-79 -1.168271049615349 2316 80-81 -1.1245333643817368 2316 82-83 -1.0863857246802588 2316 84-85 -1.1547284813812624 2316 86-87 -1.1542716306850167 2316 88-89 -1.1026908422417172 2316 90-91 -1.0921187592441086 2316 92-93 -1.0810128110027009 2316 94-95 -0.9727102543490744 2316 96-97 -0.9638786259536687 2316 98-99 -0.9881251546194072 2316 100 -1.1221021214121727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20507.0 3 3768.0 4 3528.0 5 4080.0 6 4709.0 7 5579.0 8 6822.0 9 8742.0 10 10973.0 11 15211.0 12 22301.0 13 29486.0 14 30482.0 15 35535.0 16 45276.0 17 58555.0 18 57021.0 19 36489.0 20 33625.0 21 33273.0 22 36326.0 23 42707.0 24 47711.0 25 52961.0 26 59274.0 27 68727.0 28 80734.0 29 96451.0 30 116793.0 31 145499.0 32 186478.0 33 249944.0 34 357468.0 35 565968.0 36 1011486.0 37 1759331.0 38 1468987.0 39 283756.0 40 448.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.75349703150302 17.67424781783344 13.034979413709324 24.53727573695422 2 14.458472506917783 21.947541093036733 36.64458274512488 26.9494036549206 3 19.289011204431876 27.195803026715648 29.044326372040093 24.470859396812383 4 13.41187499054165 18.37925128912875 34.48974347260784 33.71913024772176 5 13.57886256995873 36.55756402283905 33.67618293854938 16.187390468652836 6 31.26820878085122 33.24042927853438 18.675831494347587 16.815530446266813 7 25.480044909748678 31.76687347203273 22.60128542583823 20.15179619238036 8 27.551125933786956 31.20543221379304 21.760796756292628 19.482645096127378 9 27.965061975317184 14.690208132861638 19.639959306746228 37.704770585074954 10-11 24.717311800886524 25.79966205764731 26.2391325496772 23.243893591788964 12-13 27.146829914573605 23.50375306188676 26.290970254827496 23.058446768712145 14-15 25.959455291744643 22.86072662058778 24.865679681239595 26.314138406427983 16-17 23.487326336345042 25.89468396795128 27.66757885721502 22.950410838488654 18-19 24.805716646895405 25.26912767381283 28.462546510449698 21.46260916884207 20-21 24.04885154169147 25.064262282265194 28.007876658755055 22.87900951728828 22-23 22.893109215541863 26.02126421950384 25.44992601408756 25.635700550866737 24-25 23.130942351624398 26.9489036011241 24.814824930682196 25.1053291165693 26-27 24.347441197160435 24.874665350841042 25.381212110262872 25.39668134173565 28-29 23.540933665816826 24.356704856346184 27.972963931488376 24.129397546348617 30-31 25.194569754919915 24.630093643711053 26.98326009578167 23.19207650558736 32-33 22.002859452608988 25.67037218776366 27.427078663188254 24.899689696439097 34-35 21.845186651337517 26.306380881559612 27.932477437841502 23.915955029261372 36-37 24.107073473336136 25.9298281342708 26.34216287564292 23.62093551675014 38-39 24.83024462627587 24.663115844037016 25.985472445891862 24.52116708379525 40-41 23.409194161125185 25.93645075909364 26.190188266390745 24.46416681339043 42-43 25.52241275752954 25.024378897741745 25.748712947195283 23.70449539753344 44-45 25.413335697195965 24.476803222751528 26.301744667434075 23.80811641261843 46-47 23.888330958700294 24.89619652789468 28.31816573814446 22.89730677526056 48-49 24.58570514890805 25.81866560546028 27.231763775006357 22.36386547062531 50-51 22.83784204393783 26.429422051321755 26.357029634026937 24.375706270713483 52-53 22.429225622538958 26.842144649080357 24.525149156919827 26.20348057146085 54-55 23.350029047313672 26.411589825283986 24.267812777814946 25.970568349587396 56-57 24.387987573935835 24.531909209722652 24.819280098520714 26.2608231178208 58-59 23.900481979053094 24.4899412959853 26.92175015024597 24.68782657471564 60-61 24.23190413679062 25.067990354673817 26.020486411060496 24.679619097475065 62-63 21.85558425850026 25.567123707309698 26.843601609646594 25.733690424543443 64-65 21.761329226939566 25.663971331505632 26.97915233374772 25.595547107807082 66-67 23.951184831883854 25.207044356608332 25.706406437206564 25.135364374301243 68-69 24.410871910489185 24.216501474596345 25.634762550252383 25.737864064662087 70-71 22.929112187130496 24.99325273675064 26.080165246931493 25.99746982918737 72-73 24.50736662219152 24.230545887130972 26.027880341868283 25.234207148809222 74-75 24.78876082878476 23.478764199406697 26.483311891942453 25.249163079866094 76-77 23.306151053232178 24.52322656234228 27.868825380744628 24.301797003680917 78-79 23.638765420059762 24.504625056528546 27.484301310686117 24.372308212725574 80-81 22.345202557567877 24.76702665916092 27.208390812889437 25.679379970381767 82-83 22.276846392870613 25.56363215399351 25.37758248586427 26.781938967271614 84-85 22.78533454329396 25.346186577622596 25.229838419448942 26.638640459634498 86-87 23.191737285668506 23.66619732792381 26.060650628977665 27.081414757430018 88-89 23.2462971566702 23.179888156828827 27.2484149623266 26.32539972417437 90-91 23.861208585473108 23.849351211054348 26.504633763111396 25.784806440361148 92-93 22.29869466125884 24.207382234210886 27.044686657840426 26.449236446689845 94-95 21.83583276231704 24.570765761304514 27.498370367919705 26.095031108458738 96-97 23.64020062548244 23.913558465443682 26.305538536082096 26.140702372991782 98-99 23.900395945790073 23.348206200154497 26.08341247605121 26.66798537800422 100 23.345501782692683 23.830570714843713 26.78005121130739 26.04387629115621 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1388.0 1 1831.0 2 1546.0 3 752.5 4 614.0 5 601.5 6 767.5 7 1139.5 8 1609.0 9 1729.5 10 1498.0 11 1428.5 12 1580.0 13 1746.0 14 1951.0 15 2235.5 16 2637.0 17 3046.0 18 3485.0 19 4174.0 20 5074.5 21 6084.5 22 7445.5 23 9230.0 24 11387.0 25 14293.5 26 18422.0 27 23402.0 28 28843.0 29 35896.5 30 43845.0 31 51162.5 32 58437.0 33 66900.5 34 76126.5 35 83348.0 36 89932.0 37 97591.0 38 103839.5 39 111987.0 40 118539.5 41 121910.0 42 128700.0 43 144422.0 44 165431.5 45 184369.0 46 211919.5 47 254123.5 48 364979.5 49 518714.5 50 697615.5 51 642126.0 52 393057.5 53 293846.5 54 258515.5 55 228895.0 56 209225.5 57 189282.0 58 175464.0 59 160569.5 60 137792.5 61 113420.0 62 90640.5 63 70618.5 64 51968.5 65 40810.5 66 31825.5 67 22716.5 68 17078.0 69 13804.5 70 11748.0 71 10189.5 72 8427.0 73 8441.0 74 7096.5 75 5624.5 76 4333.0 77 2795.5 78 2003.0 79 1405.0 80 998.0 81 791.5 82 666.5 83 519.0 84 339.5 85 221.5 86 154.0 87 104.5 88 76.5 89 60.5 90 56.0 91 50.0 92 50.0 93 41.0 94 32.5 95 28.5 96 23.5 97 18.5 98 12.0 99 5.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11370984207295155 2 0.36783090796956636 3 0.3772433211671787 4 0.37393206802131207 5 0.3016903876857454 6 0.13767767867345845 7 0.1521626498817601 8 0.12782846186937008 9 0.2079185166825865 10-11 0.20766488878205205 12-13 0.21971221405743913 14-15 0.1505633850645011 16-17 0.1363390869761932 18-19 0.1545580244979189 20-21 0.15515686815195862 22-23 0.16261071033988816 24-25 0.17516529141634415 26-27 0.1687893678056861 28-29 0.17682091798927746 30-31 0.21824680840990665 32-33 0.21468192736350555 34-35 0.13279534158817002 36-37 0.09206692789401058 38-39 0.085557145113626 40-41 0.08484557794823765 42-43 0.07502454202198644 44-45 0.09751288253604229 46-47 0.14515970173922516 48-49 0.22209349823467936 50-51 0.17902607168003545 52-53 0.11154695969894932 54-55 0.060201400279638856 56-57 0.07474273324361481 58-59 0.10994064966223104 60-61 0.08617007920658429 62-63 0.0952372766506914 64-65 0.10450174023965864 66-67 0.10096504007109472 68-69 0.0905522057102631 70-71 0.12613760919914033 72-73 0.15014771711640296 74-75 0.19372239947211578 76-77 0.19332082196293623 78-79 0.15484687849574982 80-81 0.09083401448863472 82-83 0.13227399534818252 84-85 0.16036328533237443 86-87 0.1721922088045235 88-89 0.16027874269886294 90-91 0.18052670342486435 92-93 0.10096504007109472 94-95 0.3395161709626771 96-97 0.3414183802166856 98-99 0.38012481592602854 100 0.38168180942653185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7097011.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.559731193737456 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09906091335964 30.084991967765905 2 11.504596962996578 8.642191388448795 3 3.3153766317416697 3.735739652826474 4 1.4338536189260782 2.154206259921246 5 0.7951737050601309 1.4933255307193392 6 0.47057382703768574 1.0604775870206267 7 0.336054430364932 0.8835479849679986 8 0.25285168615645554 0.7597633091135789 9 0.18961840415794606 0.6409814660602131 >10 1.2792106025479577 9.488701294187567 >50 0.1600221134435952 4.225508810721923 >100 0.13569546126450957 10.481087340530209 >500 0.016875932030384105 4.349657767970249 >1k 0.008987847031729003 6.156482526804121 >5k 7.963915091385257E-4 2.2694852157806493 >10k+ 0.0012514723715033977 13.573851897161113 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 128773 1.8144680908624773 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 117034 1.6490604283972508 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 54017 0.7611232390650092 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 50013 0.7047051216350094 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 46411 0.6539513606502794 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 42387 0.597251434441908 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 41014 0.5779052618066958 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 39906 0.5622930554849076 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 39208 0.5524579291197379 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 37658 0.5306177487959368 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 32413 0.45671339666797756 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 27913 0.39330642153436146 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 23658 0.33335160393579777 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 20035 0.2823019437337775 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 17158 0.24176375096501895 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 16920 0.23841022650239657 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 16751 0.23602894232515634 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 15896 0.22398161704976927 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 15487 0.2182186275320695 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 15365 0.21649959398400256 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15266 0.21510464053106298 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 13976 0.19692797432609307 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 13030 0.1835984191091151 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 12332 0.1737632927439453 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 12332 0.1737632927439453 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 11598 0.16342091057770658 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 11570 0.1630263782879863 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 11533 0.1625050320479988 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 11385 0.16041964708804876 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 11093 0.156305238923823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10486 0.1477523425002441 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10255 0.14449745111005183 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10084 0.1420879860549744 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 9856 0.13887536598153788 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9577 0.13494413352325366 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 9553 0.1346059629892077 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 9536 0.13436642552759184 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 9436 0.1329573816357337 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 8538 0.12030416748684764 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 8478 0.11945874115173276 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 8278 0.11664065336801648 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 7992 0.11261078783730222 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 7625 0.10743959675418285 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7411 0.10442424282560644 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 7341 0.10343791210130573 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 7113 0.10022529202786919 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.454263351148815E-5 0.0 0.0 0.0 1.4090438918581358E-5 2 1.2681395026723223E-4 0.0 0.0 0.0 1.4090438918581358E-5 3 1.2681395026723223E-4 0.0 0.0 0.0 1.4090438918581358E-5 4 2.1135658377872038E-4 0.0 0.0 0.0 1.4090438918581358E-5 5 0.0010145116021378579 0.0 0.0 1.4090438918581358E-5 1.4090438918581358E-5 6 0.0021417467156243666 0.0 0.0 1.4090438918581358E-5 2.8180877837162717E-5 7 0.002437645932914575 0.0 0.0 2.8180877837162717E-5 2.8180877837162717E-5 8 0.0027053642723676207 1.4090438918581358E-5 0.0 2.8180877837162717E-5 2.8180877837162717E-5 9 0.0030153539285764103 4.227131675574407E-5 0.0 4.227131675574407E-5 4.227131675574407E-5 10-11 0.005100738888526451 4.227131675574407E-5 0.0 1.1976873080794156E-4 4.931653621503475E-5 12-13 0.010849637967307646 4.227131675574407E-5 0.0 1.690852670229763E-4 5.636175567432543E-5 14-15 0.017401692064447978 4.227131675574407E-5 0.0 3.099896562087899E-4 5.636175567432543E-5 16-17 0.019853428436281136 4.227131675574407E-5 0.0 4.790749232317662E-4 5.636175567432543E-5 18-19 0.020952482671930478 4.227131675574407E-5 0.0 6.058888734989983E-4 5.636175567432543E-5 20-21 0.025073936055615524 4.227131675574407E-5 0.0 0.001049737699434311 6.340697513361612E-5 22-23 0.027039552284757625 4.227131675574407E-5 0.0 0.0024165102745367026 7.045219459290679E-5 24-25 0.03682536211371237 4.227131675574407E-5 0.0 0.005326185911223753 7.045219459290679E-5 26-27 0.0693813212350946 4.227131675574407E-5 0.0 0.010765095333796156 7.045219459290679E-5 28-29 0.07680698254518697 4.227131675574407E-5 0.0 0.032337557318144215 7.045219459290679E-5 30-31 0.07834988560677164 4.227131675574407E-5 0.0 0.08932633752434652 7.045219459290679E-5 32-33 0.0794418946229617 4.227131675574407E-5 0.0 0.1912988439781198 7.045219459290679E-5 34-35 0.08024504964132084 4.227131675574407E-5 0.0 0.32645433408515223 7.749741405219747E-5 36-37 0.08179499792236478 4.227131675574407E-5 0.0 0.5224241585647817 8.454263351148815E-5 38-39 0.08328153922827511 4.227131675574407E-5 0.0 0.8140398824237415 9.158785297077882E-5 40-41 0.08497943711796417 5.636175567432543E-5 0.0 1.236985542223339 9.86330724300695E-5 42-43 0.08895998611246339 5.636175567432543E-5 0.0 1.5465877113618678 9.86330724300695E-5 44-45 0.0935675596388395 5.636175567432543E-5 0.0 1.867786311730389 1.1272351134865087E-4 46-47 0.09563885415987096 5.636175567432543E-5 0.0 2.242289042527904 1.1272351134865087E-4 48-49 0.09697744585713619 5.636175567432543E-5 0.0 2.5799170946754906 1.1272351134865087E-4 50-51 0.09895715252519688 5.636175567432543E-5 0.0 2.95148901417794 1.1272351134865087E-4 52-53 0.0996969005684224 5.636175567432543E-5 0.0 3.400255403295838 1.2681395026723223E-4 54-55 0.10197955167323258 5.636175567432543E-5 0.0 3.7984723427933256 1.4090438918581359E-4 56-57 0.10715778797581121 5.636175567432543E-5 0.0 4.219649652508641 1.4090438918581359E-4 58-59 0.108848640646041 5.636175567432543E-5 0.0 4.662279655477496 1.4090438918581359E-4 60-61 0.10987019746763814 5.636175567432543E-5 0.0 5.063983978607332 1.4794960864510426E-4 62-63 0.11082130209464239 5.636175567432543E-5 0.0 5.475791146441791 1.5499482810439494E-4 64-65 0.11146946228489713 5.636175567432543E-5 0.0 5.927586979927184 1.5499482810439494E-4 66-67 0.11234306949784917 5.636175567432543E-5 0.0 6.39692540986621 1.6204004756368562E-4 68-69 0.11337871675836489 5.636175567432543E-5 0.0 6.959944122955425 1.690852670229763E-4 70-71 0.11459753972482217 5.636175567432543E-5 0.0 7.655624318463083 1.8317570594155765E-4 72-73 0.11805674247933391 6.340697513361612E-5 0.0 8.21970685969065 1.97266144860139E-4 74-75 0.1215370808922235 7.045219459290679E-5 0.0 8.765753639102433 2.043113643194297E-4 76-77 0.12276294907814009 7.749741405219747E-5 0.0 9.358827258404983 2.1840180323801106E-4 78-79 0.12367178238838858 9.158785297077882E-5 0.0 9.931927680540442 2.2544702269730173E-4 80-81 0.12516536891375818 9.86330724300695E-5 0.0 10.57385989679317 2.4658268107517376E-4 82-83 0.12571489603158287 9.86330724300695E-5 0.0 11.321167460498511 2.8180877837162717E-4 84-85 0.128117315867201 1.0567829188936018E-4 0.0 12.015826099184572 2.8180877837162717E-4 86-87 0.13246421627358335 1.2681395026723223E-4 0.0 12.711449932936556 2.888539978309179E-4 88 0.1335632705092327 1.2681395026723223E-4 0.0 13.265655076482197 2.958992172902085E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTACA 6605 0.0 29.087713 2 TCTACAC 7970 0.0 26.648405 3 TACACTC 7595 0.0 26.451342 5 CTACACT 9695 0.0 23.160692 4 CACTCTT 9230 0.0 21.85905 7 ACTCTTT 12020 0.0 19.639294 8 ACACTCT 10435 0.0 19.467434 6 GTACTAG 1760 0.0 18.467026 1 AGTACTT 21940 0.0 17.95493 12-13 AGTACTC 2535 0.0 17.63166 5 GAGTACT 21430 0.0 17.482857 12-13 TATCACG 565 0.0 16.66878 2 GTATATA 1435 0.0 16.084394 1 CATGGGT 7390 0.0 15.733794 4 CGTTAAC 570 0.0 15.701431 1 GTATTAT 1960 0.0 15.621323 1 TAGGCAT 2840 0.0 15.406791 5 GTATAAT 1865 0.0 15.406768 1 GTATAAG 2145 0.0 15.372031 1 GTACTTT 24790 0.0 15.188775 14-15 >>END_MODULE