FastQCFastQC Report
Fri 27 May 2016
ERR765276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10358850
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA185480.17905462478943127No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT169570.16369577704088775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA427200.020.7891641
GTATAAG40000.019.5008961
CATGGGG210500.019.308734
CGTTAAC10650.018.9725321
GTACATG680650.018.564081
GTACTAG37700.018.4470461
TACATGG670100.018.3722272
CAACGCA463000.018.1151835
ACATGGG632900.018.0043223
ATCAACG471950.017.772253
AACGCAG479650.017.6113246
GTGATCG24150.017.1195498
TCAACGC499000.016.883774
TAGTACT47250.016.7069474
TAGGCAT55300.016.653925
TATCAAC532600.016.5159972
ACGCAGA502800.016.4640797
CGCAGAG505900.016.3539058
GTATATA34600.016.2971461
ATGGGAG93000.016.0162855
Produced by FastQC (version 0.11.5)