##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765276_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10358850
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94753172408134	33.0	33.0	33.0	27.0	33.0
2	31.87110625214189	33.0	33.0	33.0	27.0	33.0
3	31.902493230426156	33.0	33.0	33.0	27.0	33.0
4	35.68446632589525	37.0	37.0	37.0	33.0	37.0
5	35.716196585528316	37.0	37.0	37.0	33.0	37.0
6	35.70669331055088	37.0	37.0	37.0	33.0	37.0
7	35.63762386751425	37.0	37.0	37.0	33.0	37.0
8	35.63596991944087	37.0	37.0	37.0	33.0	37.0
9	35.63404161658871	37.0	37.0	37.0	33.0	37.0
10-11	35.59535653088905	37.0	37.0	37.0	33.0	37.0
12-13	35.545405474545916	37.0	37.0	37.0	33.0	37.0
14-15	37.1434321860052	40.0	37.0	40.0	33.0	40.0
16-17	37.17075930243222	40.0	37.0	40.0	33.0	40.0
18-19	37.129628674997704	40.0	37.0	40.0	33.0	40.0
20-21	37.213163333767746	40.0	37.0	40.0	33.0	40.0
22-23	37.18506166225015	40.0	37.0	40.0	33.0	40.0
24-25	37.15976705908474	40.0	37.0	40.0	33.0	40.0
26-27	37.10814129946857	40.0	37.0	40.0	33.0	40.0
28-29	37.052055440517044	40.0	37.0	40.0	33.0	40.0
30-31	36.98321010536884	40.0	37.0	40.0	33.0	40.0
32-33	36.88382016343513	37.0	37.0	40.0	33.0	40.0
34-35	36.78202898970446	37.0	37.0	40.0	33.0	40.0
36-37	36.701337021001365	37.0	37.0	40.0	33.0	40.0
38-39	36.60275624224697	37.0	37.0	40.0	33.0	40.0
40-41	36.51625122479812	37.0	37.0	40.0	33.0	40.0
42-43	36.38469637073614	37.0	37.0	40.0	33.0	40.0
44-45	36.254974876554826	37.0	37.0	40.0	33.0	40.0
46-47	36.09405609696057	37.0	37.0	40.0	30.0	40.0
48-49	35.8720098756136	37.0	37.0	40.0	27.0	40.0
50-51	35.780586696399695	37.0	37.0	40.0	27.0	40.0
52-53	35.628309030442566	37.0	35.0	40.0	27.0	40.0
54-55	35.462875994922214	37.0	33.0	40.0	27.0	40.0
56-57	35.301214130912214	37.0	33.0	40.0	27.0	40.0
58-59	35.141190093494934	37.0	33.0	40.0	27.0	40.0
60-61	34.96200722087877	37.0	33.0	40.0	27.0	40.0
62-63	34.74757926796893	37.0	33.0	37.0	27.0	40.0
64-65	34.55862224088581	37.0	33.0	37.0	27.0	40.0
66-67	34.368196276613716	37.0	33.0	37.0	27.0	40.0
68-69	34.18020649010266	37.0	33.0	37.0	27.0	40.0
70-71	33.988615242039415	37.0	33.0	37.0	27.0	40.0
72-73	33.78704754871438	37.0	33.0	37.0	27.0	37.0
74-75	33.58345516152855	37.0	33.0	37.0	27.0	37.0
76-77	33.40096130361961	37.0	33.0	37.0	27.0	37.0
78-79	33.255359668302944	37.0	33.0	37.0	27.0	37.0
80-81	33.09687050203449	37.0	33.0	37.0	27.0	37.0
82-83	32.98720306790811	37.0	33.0	37.0	27.0	37.0
84-85	32.85314532983874	37.0	33.0	37.0	27.0	37.0
86-87	32.74228789875324	33.0	33.0	37.0	27.0	37.0
88-89	32.63676590548179	33.0	33.0	37.0	27.0	37.0
90-91	32.53457304623583	33.0	33.0	37.0	22.0	37.0
92-93	32.45379713964388	33.0	33.0	37.0	22.0	37.0
94-95	32.35348561857735	33.0	33.0	37.0	22.0	37.0
96-97	32.285033473792936	33.0	33.0	37.0	22.0	37.0
98-99	32.18739840812445	33.0	33.0	37.0	22.0	37.0
100	32.14211799572347	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	8.0
7	140.0
8	823.0
9	1656.0
10	2503.0
11	3708.0
12	6124.0
13	10185.0
14	14454.0
15	17483.0
16	21132.0
17	25757.0
18	31809.0
19	38336.0
20	46128.0
21	55197.0
22	65451.0
23	71195.0
24	75978.0
25	85168.0
26	98495.0
27	118130.0
28	145561.0
29	183958.0
30	235964.0
31	306310.0
32	401577.0
33	531428.0
34	700729.0
35	978230.0
36	1653248.0
37	2905083.0
38	1524634.0
39	2266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69484839321018	19.384713067316124	12.851740536089673	25.068698003384014
2	16.0539081403327	21.226586091635692	37.670629094033885	25.04887667399773
3	17.90251041375733	26.321620617844477	29.532920525656408	26.24294844274179
4	12.878640216674075	17.351293610074514	37.53931465383459	32.23075151941682
5	14.133413277197493	37.43554622533739	33.65560059695196	14.775439900513158
6	31.008625474835526	37.67815925512967	17.73300125013877	13.580214019896031
7	27.11332821693528	31.98877288502102	22.791255786115254	18.10664311192845
8	26.451662105349534	32.91638550611313	20.761899245572625	19.87005314296471
9	26.991654479020355	14.692538264382629	20.2395053504974	38.076301906099616
10-11	25.447950303363793	25.367946248859667	27.415586672265746	21.768516775510797
12-13	26.035544486115736	23.35249086529875	27.175347649594308	23.436616998991198
14-15	24.021479218252992	24.387031378965812	24.615768159593003	26.975721243188193
16-17	23.77622711785918	26.694610981342414	25.438564862942098	24.090597037856305
18-19	24.103154094719137	25.988443022770042	27.055007745921234	22.853395136589587
20-21	25.465726473155957	25.31034152204753	25.780898434126883	23.443033570669623
22-23	24.481792717086833	25.12831912016918	25.867234710530774	24.522653452213213
24-25	24.113354909674836	25.728544252473995	25.96360173813655	24.194499099714626
26-27	24.365207826170234	25.662311242647423	25.761806602491227	24.210674328691116
28-29	23.80788969796646	25.553176016622857	26.444594676341804	24.19433960906888
30-31	24.45267661881866	25.417691014931897	26.107233917154915	24.022398449094524
32-33	23.98152880791895	25.4728249021437	25.677550274032413	24.868096015904946
34-35	24.543661769350862	25.39039794104288	26.242193260105395	23.823747029500865
36-37	24.494358330107808	25.401813355619314	25.759738152599432	24.34409016167345
38-39	24.542660532099855	25.007363575432628	25.9725531868362	24.477422705631312
40-41	24.346422790300142	25.394527614108465	26.203108247513768	24.05594134807762
42-43	24.921143032813518	25.185142129853087	25.8810984950048	24.012616342328595
44-45	24.363936796806346	25.50488108885765	25.608606369319254	24.52257574501675
46-47	24.078750463575673	25.247197714626246	26.067488893735856	24.60656292806223
48-49	24.771814288510736	25.556852788325003	25.573174266903244	24.09815865626102
50-51	24.07727633586961	25.83264697729961	25.420596821287372	24.66947986554341
52-53	24.169021126723504	25.45058062411703	25.496075749697916	24.884322499461547
54-55	24.47184130495553	25.409408746342173	25.626896506107684	24.491853442594618
56-57	24.39048433133979	25.070527351346584	25.300539275548605	25.238449041765023
58-59	24.056312685532085	25.135701433721813	25.87593978664803	24.932046094098073
60-61	24.164779155113816	25.279448100923123	25.702510091858628	24.853262652104434
62-63	23.76987309672975	25.371828884679182	25.502420089166034	25.355877929425034
64-65	23.85371876259023	25.117436412002508	25.70198034647157	25.32686447893569
66-67	24.303692533887762	25.243012691172336	25.511321603996727	24.94197317094318
68-69	24.436682403143433	24.941987430127146	25.40799953659455	25.213330630134873
70-71	23.729177236240165	24.990656939521365	25.741958716754414	25.538207107484055
72-73	24.338693427408565	25.094577188352424	25.617969706818798	24.948759677420213
74-75	24.56258112025978	24.960878609071703	25.567377825871567	24.909162444796955
76-77	24.056864621055386	25.332830989437262	25.586207329329387	25.02409706017797
78-79	24.18081227586166	25.396723722155002	25.516429901144775	24.906034100838568
80-81	23.791300435941167	25.161299197842187	25.88440210076195	25.162998265454696
82-83	24.04349639913641	25.36358699427045	25.427990957863067	25.164925648730076
84-85	24.06641483700145	25.408204727497154	25.163345123549163	25.362035311952237
86-87	23.70525617667793	25.092554536088173	25.474690288211583	25.727498999022313
88-89	23.836552515074228	24.874265596008783	25.581002129602638	25.70817975931436
90-91	24.151328631363924	24.945104539337645	25.42883911039717	25.474727718901264
92-93	23.874185096432086	25.125799554212236	25.304928465727638	25.695086883628036
94-95	23.339083358992603	25.227956410116924	25.742017102951493	25.69094312793898
96-97	23.887865676833897	25.108996338784923	25.374072538599002	25.629065445782174
98-99	23.520527751659	25.108604780927262	25.50615137479172	25.864716092622018
100	23.550738104006946	25.21887294995914	25.238431511487757	25.991957434546155
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	539.0
1	494.0
2	590.5
3	750.5
4	616.0
5	516.0
6	699.0
7	1338.0
8	2103.5
9	1994.0
10	1317.0
11	990.5
12	1085.0
13	1292.5
14	1467.0
15	1765.5
16	2090.5
17	2496.5
18	3132.5
19	3850.5
20	4861.0
21	6344.0
22	8302.5
23	10863.0
24	14271.5
25	19082.5
26	26484.5
27	35567.0
28	44611.0
29	57429.5
30	71886.5
31	85456.0
32	101774.0
33	118795.5
34	138218.5
35	154348.5
36	169520.0
37	191034.0
38	203538.0
39	212232.5
40	222398.5
41	230468.5
42	240174.0
43	265760.0
44	301121.0
45	328697.5
46	366624.5
47	404200.5
48	439528.0
49	467732.0
50	485337.5
51	471714.0
52	441817.0
53	437816.0
54	419505.5
55	391978.0
56	374459.0
57	347616.5
58	328985.0
59	306982.5
60	264093.0
61	214098.5
62	175648.0
63	137818.5
64	100479.5
65	84765.0
66	71882.5
67	54179.5
68	41339.5
69	33737.0
70	29870.5
71	27118.5
72	24787.5
73	27003.0
74	21360.5
75	14970.0
76	12024.0
77	10343.5
78	8493.5
79	5348.5
80	3947.0
81	3490.5
82	3626.5
83	3698.5
84	2897.5
85	1986.5
86	1469.0
87	714.5
88	310.0
89	258.5
90	204.0
91	152.0
92	118.5
93	78.5
94	48.5
95	35.0
96	23.0
97	12.0
98	4.5
99	3.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01859279746303885
2	0.006149331248159786
3	0.0065354744976517665
4	0.004199307838225285
5	0.04747631252503898
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.33048552686833E-4
18-19	0.001665242763434165
20-21	5.840416648566202E-4
22-23	0.004778522712463256
24-25	0.007196744812406782
26-27	0.005579769955159115
28-29	0.021899149036813934
30-31	0.019900857720692933
32-33	0.012704112908286153
34-35	0.013259193829430872
36-37	0.013196445551388427
38-39	0.020547647663592
40-41	0.03003229122923877
42-43	0.01744402129580021
44-45	0.01625180401299372
46-47	0.017926700357665186
48-49	0.012863396998701593
50-51	0.009740463468435202
52-53	0.026986586348870772
54-55	0.025437186560284198
56-57	0.012969586392311888
58-59	0.01588496792597634
60-61	0.016787577771663842
62-63	0.020306308132659514
64-65	0.010783050242063549
66-67	0.015175429705034825
68-69	0.00724018592797463
70-71	0.00863995520738306
72-73	0.010044551277410137
74-75	0.0037311091482162594
76-77	0.0071436501156016356
78-79	0.005512194886498019
80-81	0.002196189731485638
82-83	1.4963050917814235E-4
84-85	0.0025195847029351715
86-87	0.002196189731485638
88-89	0.0010618939361029458
90-91	6.757506866109656E-4
92-93	0.0010522403548656463
94-95	0.00333531231748698
96-97	0.0012597923514675858
98-99	0.0018341804350869065
100	0.002075519966019394
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.035885E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.18890401065013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7969228240087	33.59963832170068
2	12.98106151565942	11.212756395123183
3	3.983146241674533	5.160831620761897
4	1.708586135546581	2.951678504081957
5	0.8960806657957492	1.9350370930426033
6	0.5502656839817477	1.425922308350546
7	0.3507674812438648	1.0604484154249156
8	0.25814414632663585	0.8919170205289838
9	0.1996986189100806	0.7762288034850182
>10	1.048277707698015	8.43737173435766
>50	0.09613217201737119	2.890445826804392
>100	0.09687281725004111	9.107663489825892
>500	0.01864523174137411	5.5678779854661835
>1k	0.014273118052235455	11.656594265556855
>5k	0.0010806144901372103	2.98037195037085
>10k+	4.50256037557171E-5	0.3452162651182996
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	18548	0.17905462478943127	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	16957	0.16369577704088775	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.653581237299507E-6
2	0.0	0.0	0.0	0.0	9.653581237299507E-6
3	0.0	0.0	0.0	0.0	9.653581237299507E-6
4	0.0	0.0	0.0	9.653581237299507E-6	9.653581237299507E-6
5	0.0	9.653581237299507E-6	0.0	9.653581237299507E-6	9.653581237299507E-6
6	0.0	9.653581237299507E-6	0.0	9.653581237299507E-6	1.9307162474599015E-5
7	0.0	9.653581237299507E-6	0.0	9.653581237299507E-6	1.9307162474599015E-5
8	0.0	9.653581237299507E-6	0.0	9.653581237299507E-6	1.9307162474599015E-5
9	0.0	9.653581237299507E-6	0.0	2.896074371189852E-5	1.9307162474599015E-5
10-11	0.0	9.653581237299507E-6	0.0	5.792148742379704E-5	3.861432494919803E-5
12-13	0.0	9.653581237299507E-6	0.0	6.757506866109655E-5	7.722864989839606E-5
14-15	0.0	9.653581237299507E-6	0.0	7.722864989839606E-5	9.653581237299507E-5
16-17	0.0	9.653581237299507E-6	0.0	7.722864989839606E-5	9.653581237299507E-5
18-19	0.0	9.653581237299507E-6	0.0	8.688223113569556E-5	9.653581237299507E-5
20-21	1.4480371855949262E-5	9.653581237299507E-6	0.0	1.7376446227139111E-4	1.0618939361029458E-4
22-23	1.9307162474599015E-5	9.653581237299507E-6	0.0	4.633718993903763E-4	1.158429748475941E-4
24-25	2.4133953093248767E-5	9.653581237299507E-6	0.0	0.0013080602576540832	1.3032334670354335E-4
26-27	4.8267906186497535E-5	9.653581237299507E-6	0.0	0.002442356053036775	1.351501373221931E-4
28-29	4.8267906186497535E-5	9.653581237299507E-6	0.0	0.009798384955859	1.351501373221931E-4
30-31	4.8267906186497535E-5	9.653581237299507E-6	0.0	0.03569894341553358	1.351501373221931E-4
32-33	4.8267906186497535E-5	9.653581237299507E-6	0.0	0.0890977280296558	1.351501373221931E-4
34-35	4.8267906186497535E-5	9.653581237299507E-6	0.0	0.16102656182877445	1.351501373221931E-4
36-37	4.8267906186497535E-5	1.4480371855949262E-5	0.0	0.2734376885465085	1.351501373221931E-4
38-39	4.8267906186497535E-5	1.9307162474599015E-5	0.0	0.46048065180980513	1.351501373221931E-4
40-41	4.8267906186497535E-5	1.9307162474599015E-5	0.0	0.6830922351419317	1.351501373221931E-4
42-43	4.8267906186497535E-5	1.9307162474599015E-5	0.0	0.8824386876921666	1.351501373221931E-4
44-45	4.8267906186497535E-5	1.9307162474599015E-5	0.0	1.1179571091385627	1.351501373221931E-4
46-47	4.8267906186497535E-5	1.9307162474599015E-5	0.0	1.3840146348291558	1.4963050917814235E-4
48-49	4.8267906186497535E-5	1.9307162474599015E-5	0.0	1.6631817238400015	1.6411088103409163E-4
50-51	4.8267906186497535E-5	1.9307162474599015E-5	0.0	1.9560472446265753	1.6411088103409163E-4
52-53	4.8267906186497535E-5	1.9307162474599015E-5	0.0	2.2478315643145717	1.882448341273404E-4
54-55	4.8267906186497535E-5	3.861432494919803E-5	0.0	2.541541773459409	2.0272520598328965E-4
56-57	4.8267906186497535E-5	3.861432494919803E-5	0.0	2.8786544838471455	2.0272520598328965E-4
58-59	4.8267906186497535E-5	6.757506866109655E-5	0.0	3.250457338411117	2.0272520598328965E-4
60-61	4.8267906186497535E-5	6.757506866109655E-5	0.0	3.635080148858223	2.0272520598328965E-4
62-63	4.8267906186497535E-5	6.757506866109655E-5	0.0	4.020475245804312	2.2203236845788868E-4
64-65	4.8267906186497535E-5	6.757506866109655E-5	0.0	4.4093359784145925	2.2203236845788868E-4
66-67	4.8267906186497535E-5	1.0618939361029458E-4	0.0	4.830425191985597	2.2685915907653842E-4
68-69	4.8267906186497535E-5	1.0618939361029458E-4	0.0	5.300057438808362	2.3651274031383793E-4
70-71	4.8267906186497535E-5	1.0618939361029458E-4	0.0	5.829397085583825	2.461663215511374E-4
72-73	4.8267906186497535E-5	1.0618939361029458E-4	0.0	6.350352597054693	2.606466934070867E-4
74-75	4.8267906186497535E-5	1.1101618422894432E-4	0.0	6.857754480468392	2.703002746443862E-4
76-77	4.8267906186497535E-5	1.1584297484759408E-4	0.0	7.4008987484131925	2.703002746443862E-4
78-79	4.8267906186497535E-5	1.254965560848936E-4	0.0	7.977024476655227	2.703002746443862E-4
80-81	4.8267906186497535E-5	1.254965560848936E-4	0.0	8.60785222297842	2.703002746443862E-4
82-83	4.8267906186497535E-5	1.254965560848936E-4	0.0	9.275556649628097	2.703002746443862E-4
84-85	4.8267906186497535E-5	1.254965560848936E-4	0.0	9.922418994386444	2.703002746443862E-4
86-87	4.8267906186497535E-5	1.351501373221931E-4	0.0	10.585484875251597	2.75127065263036E-4
88	4.8267906186497535E-5	1.351501373221931E-4	0.0	11.099050570285312	2.799538558816857E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	42720	0.0	20.789164	1
GTATAAG	4000	0.0	19.500896	1
CATGGGG	21050	0.0	19.30873	4
CGTTAAC	1065	0.0	18.972532	1
GTACATG	68065	0.0	18.56408	1
GTACTAG	3770	0.0	18.447046	1
TACATGG	67010	0.0	18.372227	2
CAACGCA	46300	0.0	18.115183	5
ACATGGG	63290	0.0	18.004322	3
ATCAACG	47195	0.0	17.77225	3
AACGCAG	47965	0.0	17.611324	6
GTGATCG	2415	0.0	17.119549	8
TCAACGC	49900	0.0	16.88377	4
TAGTACT	4725	0.0	16.706947	4
TAGGCAT	5530	0.0	16.65392	5
TATCAAC	53260	0.0	16.515997	2
ACGCAGA	50280	0.0	16.464079	7
CGCAGAG	50590	0.0	16.353905	8
GTATATA	3460	0.0	16.297146	1
ATGGGAG	9300	0.0	16.016285	5
>>END_MODULE