##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765275_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6208
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.287048969072163	33.0	27.0	33.0	15.0	33.0
2	27.11855670103093	33.0	27.0	33.0	15.0	33.0
3	27.269329896907216	33.0	27.0	33.0	15.0	33.0
4	30.061855670103093	33.0	27.0	37.0	15.0	37.0
5	30.10228737113402	37.0	27.0	37.0	15.0	37.0
6	30.113885309278352	37.0	27.0	37.0	15.0	37.0
7	29.95956829896907	33.0	27.0	37.0	15.0	37.0
8	29.84890463917526	33.0	27.0	37.0	15.0	37.0
9	29.87403350515464	37.0	27.0	37.0	15.0	37.0
10-11	29.862516108247423	33.0	27.0	37.0	15.0	37.0
12-13	29.851884664948454	33.0	27.0	37.0	15.0	37.0
14-15	30.148115335051546	33.0	27.0	37.0	15.0	40.0
16-17	30.132731958762886	33.0	27.0	37.0	15.0	40.0
18-19	29.962709407216494	33.0	27.0	37.0	15.0	40.0
20-21	29.819587628865982	33.0	27.0	37.0	15.0	40.0
22-23	29.884584407216494	33.0	27.0	37.0	15.0	40.0
24-25	29.84358891752577	33.0	24.5	37.0	15.0	40.0
26-27	29.64376610824742	33.0	24.5	37.0	10.5	40.0
28-29	29.34375	33.0	22.0	37.0	6.0	40.0
30-31	29.063869201030926	33.0	22.0	37.0	6.0	40.0
32-33	28.743234536082475	33.0	22.0	37.0	6.0	40.0
34-35	28.73654961340206	33.0	22.0	37.0	6.0	40.0
36-37	28.574178479381445	33.0	22.0	37.0	6.0	40.0
38-39	28.346971649484537	33.0	22.0	37.0	6.0	40.0
40-41	28.108972293814432	33.0	22.0	37.0	6.0	40.0
42-43	27.983086340206185	33.0	22.0	37.0	6.0	40.0
44-45	27.793170103092784	33.0	22.0	37.0	6.0	40.0
46-47	27.436775128865982	33.0	15.0	37.0	6.0	40.0
48-49	27.32409793814433	33.0	15.0	37.0	6.0	40.0
50-51	27.33601804123711	33.0	15.0	37.0	6.0	40.0
52-53	27.039626288659793	33.0	15.0	37.0	6.0	40.0
54-55	26.868878865979383	33.0	15.0	37.0	6.0	37.0
56-57	26.75523518041237	33.0	15.0	37.0	6.0	37.0
58-59	26.389980670103093	33.0	15.0	37.0	6.0	37.0
60-61	26.19837306701031	33.0	15.0	37.0	6.0	37.0
62-63	26.065238402061855	27.0	15.0	37.0	6.0	37.0
64-65	25.94297680412371	27.0	15.0	37.0	6.0	37.0
66-67	25.73083118556701	27.0	15.0	37.0	6.0	37.0
68-69	25.48179768041237	27.0	15.0	33.0	6.0	37.0
70-71	25.31467461340206	27.0	15.0	33.0	6.0	37.0
72-73	25.00257731958763	27.0	15.0	33.0	6.0	37.0
74-75	24.782619201030926	27.0	15.0	33.0	6.0	37.0
76-77	24.601240335051546	27.0	15.0	33.0	6.0	37.0
78-79	24.325144974226802	27.0	15.0	33.0	6.0	37.0
80-81	24.275370489690722	27.0	15.0	33.0	6.0	37.0
82-83	24.127094072164947	27.0	15.0	33.0	6.0	37.0
84-85	23.79695554123711	27.0	15.0	33.0	6.0	37.0
86-87	23.815963273195877	27.0	15.0	33.0	6.0	37.0
88-89	23.66502899484536	27.0	15.0	33.0	6.0	37.0
90-91	23.467380798969074	27.0	15.0	33.0	6.0	35.0
92-93	23.23429445876289	27.0	15.0	33.0	6.0	33.0
94-95	23.229945231958762	27.0	15.0	33.0	6.0	33.0
96-97	23.093427835051546	27.0	15.0	33.0	6.0	33.0
98-99	22.978495489690722	27.0	15.0	33.0	6.0	33.0
100	22.854864690721648	27.0	15.0	33.0	6.0	33.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	0.0
7	0.0
8	13.0
9	55.0
10	122.0
11	170.0
12	193.0
13	193.0
14	179.0
15	157.0
16	164.0
17	154.0
18	131.0
19	127.0
20	108.0
21	117.0
22	104.0
23	123.0
24	121.0
25	127.0
26	142.0
27	166.0
28	212.0
29	243.0
30	310.0
31	345.0
32	394.0
33	415.0
34	442.0
35	408.0
36	424.0
37	294.0
38	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.26554946825652	20.834676119883984	11.601675797615211	25.29809861424428
2	13.949742268041238	25.885953608247426	33.569587628865975	26.59471649484536
3	17.8479381443299	31.072809278350515	26.91688144329897	24.162371134020617
4	12.854381443298967	21.45618556701031	32.168170103092784	33.52126288659794
5	12.355025773195877	41.623711340206185	30.13853092783505	15.882731958762886
6	30.15463917525773	38.01546391752577	16.865335051546392	14.964561855670103
7	25.370489690721648	35.196520618556704	20.32860824742268	19.10438144329897
8	26.949097938144327	35.21262886597938	18.975515463917525	18.862757731958762
9	26.61082474226804	19.039948453608247	18.137886597938145	36.21134020618557
10-11	23.195876288659793	30.541237113402065	24.065721649484537	22.197164948453608
12-13	26.200064432989688	27.923646907216494	23.79188144329897	22.084407216494846
14-15	24.589239690721648	27.271262886597935	23.276417525773198	24.863079896907216
16-17	22.108569587628864	29.6875	25.42686855670103	22.7770618556701
18-19	23.44555412371134	28.9867912371134	26.361146907216494	21.206507731958762
20-21	22.6840663766715	28.250362493958438	26.123731271145477	22.941839858224586
22-23	21.781859191235704	29.160625100692766	24.28709521507975	24.770420492991786
24-25	22.32516919110538	28.923622300999035	24.468256525942635	24.282951981952948
26-27	23.38059941991621	26.522719948436997	24.291008701256846	25.805671930389945
28-29	23.074444086368032	26.78859168546568	26.393812439574603	23.743151788591685
30-31	23.469223332259105	27.247824685787947	25.346438930067677	23.93651305188527
32-33	21.17305833064776	27.239767966484045	26.216564614888817	25.370609087979375
34-35	21.86769800982999	26.96801224720006	26.63766014019821	24.526629602771735
36-37	24.041250402835963	25.870125684821137	25.77344505317435	24.315178859168547
38-39	24.22655494682565	25.60425394779246	25.16113438607799	25.0080567193039
40-41	23.348372542700613	26.345472123751208	25.966806316467935	24.339349017080245
42-43	24.830808894618112	25.12890750886239	25.692877860135354	24.347405736384147
44-45	24.645504350628425	25.233644859813083	25.080567193038995	25.040283596519497
46-47	22.48630357718337	26.047373509506926	27.247824685787947	24.218498227521753
48-49	24.43602964872704	25.46728971962617	26.716081211730586	23.38059941991621
50-51	22.36545278762488	26.587173702868196	25.749274895262648	25.29809861424428
52-53	22.0673541733806	26.65968417660329	24.38768933290364	26.885272317112474
54-55	22.59909764743796	26.90138575572027	23.69481147276829	26.804705124073475
56-57	23.477280051563003	25.451176281018366	24.411859490815342	26.65968417660329
58-59	23.145089825183277	24.989929912188835	25.328284862644	26.53669539998389
60-61	24.065420560747665	26.055430228810827	23.992910087012568	25.88623912342894
62-63	21.30830580842665	26.407798276000964	25.811649077579958	26.472246837992426
64-65	21.52581970514783	25.956658342060745	25.811649077579958	26.705872875211472
66-67	23.606187560425397	25.676764421527555	23.888172736061875	26.828875281985177
68-69	23.942640779827602	24.869088858454845	23.902360428582938	27.285909933134615
70-71	22.421849822752176	26.06348694811473	23.493393490170803	28.021269738962296
72-73	24.176266817046642	25.82776121807782	23.93458470957867	26.061387255296864
74-75	24.323235578472445	24.30712213986465	24.782468578794713	26.587173702868196
76-77	23.332259104092813	25.66065098291975	26.35352884305511	24.653561069932323
78-79	23.9668089905744	25.408845565133326	24.50656569725288	26.117779747039393
80-81	22.768288752819853	25.797615211086043	25.153077666774088	26.281018369320012
82-83	22.577527184857026	26.49214659685864	24.00322190898107	26.927104309303264
84-85	22.75034238298558	26.738097156207203	23.572061548376702	26.939498912430516
86-87	23.55594940787884	25.183275598163217	23.894304358334004	27.36647063562394
88-89	22.756565168358307	24.923473497663927	25.08458192363461	27.235379410343164
90-91	23.50382601691502	25.67055980668546	24.252919855014095	26.57269432138542
92-93	22.796842274850977	25.83373610439826	24.32737232157242	27.042049299178345
94-95	21.635118807893676	25.67055980668546	25.428916633105114	27.265404752315746
96-97	23.74546919049537	25.509464357631895	23.890455094643574	26.854611357229157
98-99	22.752899484536083	24.412048969072163	24.887242268041238	27.947809278350515
100	23.147551546391753	25.46713917525773	24.887242268041238	26.498067010309278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	1.5
3	1.0
4	0.5
5	0.5
6	1.5
7	1.0
8	0.5
9	0.5
10	1.0
11	1.5
12	0.5
13	0.0
14	1.0
15	1.5
16	0.5
17	2.0
18	4.0
19	5.0
20	6.0
21	8.5
22	13.0
23	11.5
24	8.0
25	10.0
26	14.5
27	22.0
28	26.5
29	41.0
30	52.5
31	51.0
32	62.5
33	70.0
34	72.0
35	83.0
36	94.5
37	108.5
38	110.0
39	106.5
40	121.5
41	129.5
42	127.0
43	142.0
44	160.0
45	171.0
46	193.5
47	219.5
48	280.0
49	361.0
50	461.0
51	453.0
52	324.5
53	265.0
54	240.5
55	197.0
56	166.0
57	160.0
58	160.5
59	145.5
60	130.5
61	113.0
62	96.0
63	79.5
64	50.0
65	43.5
66	41.5
67	32.0
68	27.0
69	20.0
70	21.0
71	19.0
72	10.0
73	9.5
74	10.0
75	8.5
76	6.5
77	4.5
78	3.5
79	3.5
80	1.5
81	0.5
82	0.5
83	1.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03221649484536082
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.01610824742268041
22-23	0.01610824742268041
24-25	0.03221649484536082
26-27	0.03221649484536082
28-29	0.03221649484536082
30-31	0.03221649484536082
32-33	0.03221649484536082
34-35	0.04027061855670103
36-37	0.03221649484536082
38-39	0.03221649484536082
40-41	0.03221649484536082
42-43	0.03221649484536082
44-45	0.03221649484536082
46-47	0.03221649484536082
48-49	0.03221649484536082
50-51	0.03221649484536082
52-53	0.03221649484536082
54-55	0.03221649484536082
56-57	0.03221649484536082
58-59	0.02416237113402062
60-61	0.03221649484536082
62-63	0.02416237113402062
64-65	0.02416237113402062
66-67	0.03221649484536082
68-69	0.02416237113402062
70-71	0.03221649484536082
72-73	0.02416237113402062
74-75	0.03221649484536082
76-77	0.03221649484536082
78-79	0.02416237113402062
80-81	0.03221649484536082
82-83	0.008054123711340205
84-85	0.02416237113402062
86-87	0.02416237113402062
88-89	0.01610824742268041
90-91	0.008054123711340205
92-93	0.01610824742268041
94-95	0.008054123711340205
96-97	0.008054123711340205
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6208.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.72551546391753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.21740727139185	84.40721649484536
2	2.387073081160485	4.1881443298969065
3	0.4957767168564084	1.3047680412371134
4	0.29379360998898274	1.0309278350515463
5	0.14689680499449137	0.6443298969072164
6	0.07344840249724569	0.3865979381443299
7	0.05508630187293427	0.3382731958762887
8	0.0	0.0
9	0.01836210062431142	0.14497422680412372
>10	0.2754315093646713	4.800257731958763
>50	0.03672420124862284	2.7545103092783503
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	88	1.4175257731958764	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	83	1.3369845360824741	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	35	0.5637886597938144	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	30	0.4832474226804124	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	30	0.4832474226804124	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	29	0.46713917525773196	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	26	0.41881443298969073	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	24	0.3865979381443299	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	17	0.273840206185567	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	15	0.2416237113402062	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	15	0.2416237113402062	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15	0.2416237113402062	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	14	0.22551546391752575	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	13	0.20940721649484537	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	12	0.19329896907216496	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	12	0.19329896907216496	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	11	0.17719072164948454	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.14497422680412372	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	7	0.11275773195876287	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	7	0.11275773195876287	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	7	0.11275773195876287	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.09664948453608248	0.0
30-31	0.0	0.0	0.0	0.19329896907216496	0.0
32-33	0.0	0.0	0.0	0.24162371134020616	0.0
34-35	0.0	0.0	0.0	0.3624355670103093	0.0
36-37	0.0	0.0	0.0	0.41881443298969073	0.0
38-39	0.0	0.0	0.0	0.6604381443298969	0.0
40-41	0.0	0.0	0.0	1.1034149484536082	0.0
42-43	0.0	0.0	0.0	1.288659793814433	0.0
44-45	0.0	0.0	0.0	1.4739046391752577	0.0
46-47	0.0	0.0	0.0	1.8524484536082473	0.0
48-49	0.0	0.0	0.0	2.1182345360824746	0.0
50-51	0.0	0.0	0.0	2.4162371134020617	0.0
52-53	0.0	0.0	0.0	2.6578608247422677	0.0
54-55	0.0	0.0	0.0	2.9075386597938144	0.0
56-57	0.0	0.0	0.0	3.237757731958763	0.0
58-59	0.0	0.0	0.0	3.431056701030928	0.0
60-61	0.0	0.0	0.0	3.6887886597938144	0.0
62-63	0.0	0.0	0.0	3.9787371134020617	0.0
64-65	0.0	0.0	0.0	4.308956185567011	0.0
66-67	0.0	0.0	0.0	4.615012886597938	0.0
68-69	0.0	0.0	0.0	5.066043814432989	0.0
70-71	0.0	0.0	0.0	5.49291237113402	0.0
72-73	0.0	0.0	0.0	5.782860824742268	0.0
74-75	0.0	0.0	0.0	6.346649484536083	0.0
76-77	0.0	0.0	0.0	6.741301546391753	0.0
78-79	0.0	0.0	0.0	7.111791237113402	0.0
80-81	0.0	0.0	0.0	7.498389175257731	0.0
82-83	0.0	0.0	0.0	8.013853092783505	0.0
84-85	0.0	0.0	0.0	8.392396907216494	0.0
86-87	0.0	0.0	0.0	8.96423969072165	0.0
88	0.0	0.0	0.0	9.35889175257732	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE