FastQCFastQC Report
Fri 27 May 2016
ERR765273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7258503
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA194370.26778248903389584No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT173780.2394157583182097No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG76500.10539363281932927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20100.020.1269511
GTACATA23550.018.7580431
GTATTAG32200.017.7624991
GTACAAG38200.017.65061
CATGGGG159800.017.6336294
TAGTACT26300.017.3267144
GTACATG545450.017.0928421
TACATGG535350.016.6332382
ACATGGG507250.015.6846123
GTATAAT17300.015.3208751
CTAGTAC30400.015.2958033
GTATCAA390900.015.0123431
CCTAGTA30750.014.9704872
GTATAAG24400.014.8648191
ATGGGTA39650.014.7765555
ATTGCGT10450.014.683976
TACATGA33100.014.4695512
CTAACGC7100.014.4082153
GTACTGT42800.014.1235996
GTATAAA75100.014.1172371
Produced by FastQC (version 0.11.5)